Institute of Oncology, Lj. (OILJ)
-
Imunokemično odkrivanje antigena PCN in Ki-67 na celičnih vzorcih raka dojke : magistrska nalogaSrebotnik-Kirbiš, IrenaBackground: Assessment of breast cancer proliferative activity before surgery could help in planning effective individual treatment protocols, particulary in planning neo-adjuvant chemoterapy. In the ... present study, we are aiming to find out whether it is possible to immunochemically assess the proliferative activity of tumor on fine needle aspirates before operation. The preparation and fixation of the cell samples of immunochemical methods have not been standarized and, in addition to that, they differ from the preparation of tissue samplesection of two most important proliferative antigens, PCNA and Ki-67, in breast cancer cell samples. Out particular attention has been paid to detection of these antigens in diagnostic Papanicolaou stained fine needle aspirates. Materials and Methods: The cytospared. Part of them were fixed in Delaunayćs fixative or its modification and stained according to Papanicolaou.Others were fixed by Mercofix, methanol, acetone or glutaraldehyde and kept in methanol. Immunochemical detection of PCNA and Ki-67 antigens was carried out by avidin-biotin peroxidase method with PC10 and MIB-1 monoclonal antibodies, respectively. The percentage oIB-1 positive cells was evaluated semiquantitatively and was compared to the S phase fraction calculated from MCF-7 flow cytometric DNA histogram. Results; On the samples stained by Papanicolaoućs procedure antigen Ki-67 could be immunochemically detected onrowaves was not required for samples fixed in methanol. All other fixation methods had a negative effect on the Ki-67 antigen detection.(Abstract truncated at 2000 characteres.)Type of material - master's thesisPublication and manufacture - Ljubljana : [I. Srebotnik Kirbiš], 1998Language - slovenianCOBISS.SI-ID - 1861140
Author
Srebotnik-Kirbiš, Irena
Other authors
Us-Krašovec, Marija
Topics
Breast neoplasms |
Immunology |
Antigens, neoplasm |
Poliferating cell nuclear antigen |
Ki-67 antigen |
Immunochemistry |
Dojka, novotvorbe |
Imunologija |
Antigeni, novotvorbe |
Proliferajoče celice, jedrni antigen |
Ki-67 antigen |
Imunokemija
Call number – location, accession no. ... |
Copy status | Reservation |
---|---|---|
Knjižnica N MGR SREBOTNIK-Kirbiš Imunokemično 0000000047 IN: 0011345 Knjižnica N MGR SREBOTNIK-Kirbiš Imunokemično 47 IN: 0011345 |
available - outside loan, loan period: 7 days
|
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Srebotnik-Kirbiš, Irena | 19141 |
Source: Personal bibliographies
and: SICRIS
Select pickup location:
Material pickup by post
Delivery address:
Address is missing from the member's data.
The address retrieval service is currently unavailable, please try again.
By clicking the "OK" button, you will confirm the pickup location selected above and complete the reservation process.
By clicking the "OK" button, you will confirm the above pickup location and delivery address, and complete the reservation process.
By clicking the "OK" button, you will confirm the address selected above and complete the reservation process.
Notification
Automatic login and reservation service currently not available. You can reserve the material on the Biblos portal or try again here later.
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
Pickup location | Material status | Reservation |
---|
Reservation in progress
Please wait a moment.
Reservation was successful.
Reservation failed.
Reservation...
Membership card:
Pickup location: