Mammalian axon growth has mechanistic similarities with axon regeneration. The growth cone is an important structure that is involved in both processes, and GAP-43 (growth associated protein-43 kDa) ...is believed to be the classical molecular marker. Previously, we used growth cone phosphoproteomics to demonstrate that S96 and T172 of GAP-43 in rodents are highly phosphorylated sites that are phosphorylated by c-jun
N
-terminal protein kinase (JNK). We also revealed that phosphorylated (p)S96 and pT172 antibodies recognize growing axons in the developing brain and regenerating axons in adult peripheral nerves. In rodents, S142 is another putative JNK-dependent phosphorylation site that is modified at a lower frequency than S96 and T172. Here, we characterized this site using a pS142-specific antibody. We confirmed that pS142 was detected by co-expressing mouse GAP-43 and JNK1. pS142 antibody labeled growth cones and growing axons in developing mouse neurons. pS142 was sustained until at least nine weeks after birth in mouse brains. The pS142 antibody could detect regenerating axons following sciatic nerve injury in adult mice. Comparison of amino acid sequences indicated that rodent S142 corresponds to human S151, which is predicted to be a substrate of the MAPK family, which includes JNK. Thus, we confirmed that the pS142 antibody recognized human phospho-GAP-43 using activated JNK1, and also that its immunostaining pattern in neurons differentiated from human induced pluripotent cells was similar to those observed in mice. These results indicate that the S142 residue is phosphorylated by JNK1 and that the pS142 antibody is a new candidate molecular marker for axonal growth in both rodents and human.
海洋再解析データを用いたアカイカ好適生息域モデルの構築とその利用 五十嵐, 弘道; 淡路, 敏之; 石川, 洋一 ...
JAMSTEC Report of Research and Development,
2014, Volume:
18
Journal Article
Open access
文部科学省「気候変動適応研究推進プログラム」(RECCA: Research Program on Climate Change ...Adaptation)の研究課題「気候変動に伴う水産資源・海況変動予測技術の革新と実利用化」プロジェクトで開発した,海洋再解析データを用いたアカイカ好適生息域モデルにより,夏季における北太平洋日付変更線付近のアカイカ漁場において日々変動するアカイカ好適生息域を精度よく推定することが可能となった.さらに,この成果を,インターネットと衛星通信を介して配信するウェブシステムを構築し,操業中のイカ釣り漁船にリアルタイム配信する実証試験を行った.その結果,イカ釣り漁船が,本プロジェクトで作成したアカイカ漁海況情報を利用することにより,漁場探査の効率化や燃油消費ひいては二酸化炭素排出量削減につながったことが,聞き取り調査から明らかとなり,本プロジェクトの成果が実利用上も有益であることが示された.