The Japan Agricultural Standard (JAS) revised in 2001 requires correct indication of species and origin of marine products. PCR-based DNA analysis have been focused on species identification in ...various marine products. We established methods for identifying three Perciforms species, Japanese sea bass Lateolabrax japonicus. Chinese sea bass Lateolabrax sp. and Nile perch Lates niloticus, by PCR-restriction fragment length polymorphism (RFLP) analysis and multiplex-PCR using species-specific primers. In PCR-RFLP, 377-bp long DNA fragments of the mitochondrial cytochrome b gene were amplified and cleaved by combination of two restriction enzymes Ssp I and Hha I or Ssp I and Mse I, indicating species-specific restriction fragment patterns for these species. In addition, species-specific primer sets were also designed by based on unique nucleotide substitution in each species, which showed different size of PCR-products among these species.
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