Herbal utilization, as an antidiabetes agent, is an interesting topic to find acceptable herbal drugs to decrease blood glucose levels. The aim of this review is to evaluate the potency of selected ...herbal medicines to reduce blood glucose levels and to identify the chemical compounds responsible for reducing glucose. The mechanisms of action of different herbal medicines used might be also different. The reduction of blood glucose levels by Aloe vera, Andrographis paniculata, and Trigonella foenum-graecum through minimum 3 mechanisms of action, such as increased GLP-1 secretion and inhibited amylase, glucosidase, and SGLT 2. While Andrographis paniculata has more than 4 mechanisms of action, such as increasing GLP secretion, activating PPAR ɣ-receptor, and also inhibiting amylase, glucosidase, and SGLT 2 but it did not inhibit DPP 4 in diabetic patients.
Ocimum aristatum, commonly known as O. stamineus, has been widely studied for its potential as an herbal medicine candidate. This research aims to compare the efficacy of water and 100% ethanolic ...extracts of O. stamineus as α-glucosidase inhibitors and antioxidants, as well as toxicity against zebrafish embryos. Based on the study findings, water extract of O. stamineus leaves exhibited superior inhibition activity against α-glucosidase, ABTS, and DPPH, with IC50 values of approximately 43.623 ± 0.039 µg/mL, 27.556 ± 0.125 µg/mL, and 95.047 ± 1.587 µg/mL, respectively. The major active compounds identified in the extract include fatty acid groups and their derivates such as linoleic acid, α-eleostearic acid, stearic acid, oleanolic acid, and corchorifatty acid F. Phenolic groups such as caffeic acid, rosmarinic acid, 3,4-Dihydroxybenzaldehyde, norfenefrine, caftaric acid, and 2-hydroxyphenylalanine and flavonoids and their derivates including 5,7-Dihydroxychromone, 5,7-Dihydroxy-2,6-dimethyl-4H-chromen-4-one, eupatorin, and others were also identified in the extract. Carboxylic acid groups and triterpenoids such as azelaic acid and asiatic acid were also present. This study found that the water extract of O. stamineus is non-toxic to zebrafish embryos and does not affect the development of zebrafish larvae at concentrations lower than 500 µg/mL. These findings highlight the potential of the water extract of O. stamineus as a valuable herbal medicine candidate, particularly for its potent α-glucosidase inhibition and antioxidant properties, and affirm its safety in zebrafish embryos at tested concentrations.
Tooth agenesis in the reduction of tooth number which includes hypodontia, oligodontia and anodontia is caused by disturbances and gene mutations that occur during odontogenesis. To date, several ...genetic mutations that unlock the causes of non-syndromic tooth agenesis are being discovered; these have been associated with certain illnesses because tooth development involves the interaction of several genes for tooth epithelium and mesenchyme odontogenesis. Mutation of candidate genes PAX9 and MSX1 have been identified as the main causes of hypodontia and oligodontia; meanwhile, AXIN2 mutation is associated with anodontia. Previous study using animal models reported that PAX9-deficient knockout mice exhibit missing molars due to an arrest of tooth development at the bud stage. PAX9 frameshift, missense and nonsense mutations are reported to be responsible; however, the most severe condition showed by the phenotype is caused by haploinsufficiency. This suggests that PAX9 is dosage-sensitive. Understanding the mechanism of genetic mutations will benefit clinicians and human geneticists in future alternative treatment investigations.
Orthosiphon stamineus Benth (O. stamineus) leaves are herb plant parts that can act as an antioxidant and α-glucosidase inhibitor. Finding the best O. stamineus extract that serves as an antioxidant ...and α-glucosidase inhibitory agent is an essential requirement. Additionally, a clustering analysis based on FTIR spectra should be performed using principal component analysis (PCA) and partial least squares (PLS). Based on this study, the 40% ethanolic extract of O. stamineus leaves is a potent extract as an antioxidant and α-glucosidase inhibitory agent. Whereas 20% ethanolic extract of O. stamineus leaves is only applied as an α-glucosidase agent. Furthermore, discrimination analysis of O. stamineus leaf extracts showed that FTIR-based analysis can discriminate nicely each water, 60%, 80%, and 100% ethanolic extracts. This study reported that the obtained model has the determination coefficient of R
2
X: 0.991, R
2
Y: 0.964, and Q
2
Y: 0.946, which showed a good model and a good prediction. However, the classification method did not distinguish clearly between 20% and 40% ethanolic extracts. 20% ethanolic extract of O. stamineus leaves is always paired with 40% ethanolic extract of O. stamineus. Furthermore, several functional groups from O. stamineus leaf extracts contribute toward both biological activities including alkane groups, carbonyl groups, methylene groups, ester groups, and alkyl di-substitutions. Based on this study, quality control of potent extract as an antioxidant and as an α-Glucosidase Inhibitor should be conducted using a specific marker-based analysis.
Neonatal teeth erupt during the neonatal period and natal teeth are the presence of teeth since birth. While rare, natal teeth and neonatal teeth can have a significant impact on breastfeeding. ...Neonatal teeth are less common, and although its exact etiology is still unknown, it can cause difficulties in breastfeeding to the mother and may eventually lead to discontinuation of breastfeeding. Other associated possible complications include tooth aspiration and sublingual ulceration. This paper was aimed to discuss the clinical features, complications, and management of neonatal tooth, in addition to its impact on breastfeeding and role in sublingual ulcer formation.
We present a baby girl who had a neonatal tooth with sublingual ulceration (Riga-Fede disease), which resulted in a difficulty to breastfeed for the baby and nipple pain to the mother. Following the extraction of the baby's tooth, she immediately continued breastfeeding, and her tongue ulcer healed well.
Extraction of the neonatal tooth promoted rapid healing of oral ulcers and the reestablishment of breastfeeding.
Tooth agenenesis is reduction of tooth number caused by congenitally failure of tooth development causing hypodontia and oligodontia. The prevalence, pattern and distribution are varies by ...populations. The aim of this study was to identify the pattern and distribution of tooth agenesis in permanent dentition among Malaysian population. Patients were in the range of 12 until 60 years old has missing average of 2 teeth missing. Excluding third molar, the prevalence of population under study was 1.005%. Mandibular and/or lateral incisors (28%) were identified as the most common missing tooth. There were no significant difference between missing tooth between male and female (p=0.265). More missing teeth were observed at maxilla jaw (54.67%), right side (54.67%), posterior region (53.33%) and missing unilaterally (71.42%). Some tooth anomalies such as peg shaped tooth (8.57%) and microdontia (5.71%) also presented. In conclusion, the prevalence studied population was lower than normal range and previous studies conducted in Malaysian but still may presumably represent group of population under study, while mild hypodontia is considered as the most common type of tooth agenesis. The most common missing tooth is lateral incisors whereas mild hypondontia is identified the most common tooth agenesis.
Hypodontia is characterized by the absence of one to six teeth. Malaysia has a high prevalence of hypodontia (2.8%). This study aimed to investigate the MSX1 mutation with clinical variability in ...Malaysian hypodontia families and to correlate the findings a previous study of the PAX9 mutation. Materials and methods: We re-examined seven individuals from two families of the previous PAX9 study. Orthophantomogram (OPG) and intraoral photos were re-assessed. Saliva was collected for genetic analysis. Direct sequencing was done on exons 1 and 2 of MSX1 and exons 2 and 3 of PAX9. Results: In family 1, three out of five members are affected. The mother has posterior hypodontia, while the daughters have anterior hypodontia. Point mutations on exon 1 of MSX1 (c.599C＞T, c.732G＞A) and on exon 3 of PAX9 (c.477delG, c.480delT) were identified on 1B (mother) and 1D (second daughter). Her carrier-son (1E) exhibited c.597C＞T, c.730G＞A on exon 1 of MSX1 and c.273T＞G on exon3 of PAX9. In family 2, the daughter has a missing lower premolar with a point mutation on exon 1 of MSX1 (c.730G＞A). A similar point mutation in her nonhypodontia father on exon 2 of PAX9 (c.628C＞T) was observed. Conclusion: Mutation of MSX1 is observed in familial hypodontia; both genes MSX1 and PAX9 are needed to manifest hypodontia whereby PAX9 is the predominant gene mutation.
To analyze saliva and tongue coating pH in healthy patients with physiological halitosis before and after usage of 5 different types of mouthwash available in Malaysia. A quasi-experimental study was ...conducted among preclinical IIUM dental students aged 18 to 21 years old. Organoleptic evaluation was conducted by the operator to rank their organoleptic score followed by self-assessment by the participants. Saliva and tongue coating pH we measured respectively beforehand, immediately after and 30 minutes after rinsing with 5 different mouthwashes using a digital pH meter and pH strip indicator respectively; 100 participants were assigned into 5 groups, with 20 samples for each brand. Intraclass correlation coefficient between examiners ranged from 0.76-1.0. Data were analyzed statistically by repeated measure ANOVA test for each brand of mouthwash. Multivariate Analysis of Covariance (MANCOVA) + test was also used to statistically analyze different pH trends among the five brands of mouthwashes. Significant increase of salivary pH was observed after using MW2 (p<0.001) and MW4 (p=0.015). Significant reduction in salivary pH was observed when using MW5 (p=0.001). For tongue coating pH, significant reduction was observed after using MW2 (p<0.0010), MW3 (p=0.012), and MW4 (p=0.001). However, there was a significant increase in tongue coating pH after using MW5 (p=0.0125). Rinsing with mouthwashes MW2, MW3 and MW4 decreased tongue coating pH, which leads to reduced physiological halitosis
Dens evaginatus (DE) is a developmental anomaly. It is an extra cusp or tubercle that protrudes from the occlusal surface of posterior teeth, as well as the lingual surface of anterior teeth. ...Tubercles are susceptible to pulp exposure from wear or fracture because of malocclusion;leading to pulpal complications early after eruption.DE may also complicate the process of daily routine oral health care. A 13-year-old girl presented to our polyclinic with sinus track at the posterior lower left buccal gingiva forthe past1 year. All 35 teeth were sound. At the lingual side, there was an accessory tooth in close relation to tooth 35 covered with supra-gingival inflammation. Electric pulp test (EPT) showed that tooth 35 was already non-vital. Intraoral periapical (IOPA) radiograph and cone beam computed tomography (CBCT) scan showed an abnormalappearing root which shows DE with radiolucency at the periapical area. Root canal treatment was performed using crown down pressureless (CDP) technique and obturation was done using lateral condensation technique. The tooth was restored using composite restoration. DE is a rare condition. It is quite a challenge for dental practitioner since the diagnosis is difficult and the treatment options are limited. In the case of DE with necrotic pulp and periapical abscess, root canal treatment is a treatment of choice. We have to accept and appreciate any gift from Allah SWT whether it is good or bad, no equal divine creation except from Allah SWT.Therefore we need to take care of our oral hygiene to prevent diseases. In every disease, there is a cure; we thus need to try to do the best to find the cure and to not easily get rid of the tooth and replace it with a denture. Early diagnosis of DE can lead to proper treatment which can result in high success rate if it is followed by adequate restoration.
PDF
