Customer requirement preference is an important part of customer satisfaction. In view of similar case retrieval technology for existing product level, in the process of solving similar cases, there ...is no consideration for customer requirement preference. This article proposes a similar case solution method considering customer requirement preference. First, we deal with the expression of customer requirements and transform them into operable parameter forms according to the mapping model. Second, the preference graph is used to analyze the customer’s requirement preference, to determine the preference weight, and to weigh the final weight of the requirement node with the initial weight determined by the fuzzy analytic hierarchy process. Finally, the similarity degree solving model of requirement node and product case attribute parameters is established. By integrating the weights of the above-mentioned nodes, the similarity of the product case is obtained, and a more satisfied case of the customer is obtained. Taking the automated guided vehicle car product as an example, the effectiveness of the proposed method is verified.
In view of the dynamic change of customer requirements (CRs) during the process of product use, in this paper we propose a Bayesian Nash equilibrium configuration model for product variant design ...driven by CRs. By analyzing CRs, the complete variant requirements of the products can be obtained. Combined with modularization and parameterization variant design methods, a parametric variant instance is proposed. Since cost and delivery time are affected by the product variant design, firms and customers are established as two decision-making bodies, and Bayesian Nash theory is introduced to the product configuration. The theory takes the product cost and customer satisfaction as the payoff function of the game, and based on the threshold value search of the customer satisfaction it determines the strategy set of the two parties. The Nash equilibrium solution equation is established and solved by a simulated annealing algorithm. The optimal product configuration scheme satisfying the interests of both sides of the game is obtained. Finally, the automatic guided vehicle (AGV) is taken as an example to illustrate the effectiveness and practicability of the method.
Bacterial pathogens and antibiotic resistance genes (ARGs) are extensively disseminated into the environment via hospital wastewater (HWW), as it contains large quantities of feces from resident ...patients. However, studies on the antibiotic resistome and pathogenic bacteria from the gut of resident patients within the hospital wastewater treatment plant (hWWTP) are limited. Here, we examined and compared the occurrence and abundance of ARGs, mobile genetic elements (MGEs), metals, and bacterial communities from the feces of patients in a typical hWWTP system and determined the pathogenic hosts responsible for transferring ARGs. There were 176 ARGs and 43 MGEs detected in the feces of hospitalized patients, 129 genes were persistent, and 88 genes were enriched after HWW treatment, particularly for the blaVEB, blaNDM, and class 1 integron (intI1), with an average of 659-fold, 202-fold, and seven-fold enrichment, respectively. MGEs, especially Is613, in the feces of hospitalized patients were exceptionally abundant and even surpassed the abundance of total ARGs, which explained the persistence of ARGs in hWWTPs due to possible gene mobilization events. Bacteroidetes and Firmicutes were the most abundant phyla in these feces, accounting for 81 % of the total gut microbiota, while Epsilonbacteraeota and Proteobacteria dominated the hWWTPs. Additionally, 54 possible bacterial pathogens were found in the hospital environment, including four “ESKAPE” pathogens and 14 cancer-related pathogens. Many of them were strongly associated with different types of ARGs. Notably, Bacteroides was the major potential ARG-harboring pathogenic genus, as determined by the network analysis, and was highly abundant after the treatment. The altered microbial community was the major contributing factor shaping antibiotic resistome. This study might provide a comprehensive insight into the distribution profiles of ARGs and pathogens from the gut of inpatients throughout the HWW treatment system, which could be used as a reference for optimizing HWW treatment and monitoring public risk.
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•Dynamics of antibiotic resistance and pathogenic bacteria were assessed in the hWWTP system.•High prevalence of ARGs and bacterial pathogens from the feces of patients was found across hWWTP.•Bacterial community contributed the most to shape the ARG dynamics in hospital environment.•Bacteroides was the major potential ARG-harboring pathogenic taxon in the hWWTP system.
The rapid development of portable and wearable electronic devices is responding to the urgent demand for high-efficiency flexible energy storage devices. Flexible supercapacitors, showing long cycle ...life, high power density, and good safety, are considered ideal candidates. Nevertheless, the relatively low energy density restricts their practical applications. With a large dielectric constant of 18–46, Ta
2
O
5
-based materials typically exhibit excellent electron-binding ability, which is critical for enhancing the energy density of supercapacitors. In this work, the free-standing β-Ta
2
O
5
/single-walled carbon nanotubes (SWCNTs) composite film was prepared, with a high β-Ta
2
O
5
loading of over 70%. By anchoring β-Ta
2
O
5
nanoparticles onto the surface of SWCNTs, the system’s flexibility and conductivity were significantly enhanced, which also facilitated the intercalation electrodynamics of metal cations. As a result, the flexible β-Ta
2
O
5
/SWCNTs film exhibits excellent Li-ion storage performance, with a high volumetric specific capacitance of 392.3 F cm
−3
at the scan rate of 10 mV s
−1
and 198.9 F cm
−3
at 500 mV s
−1
. In addition, the asymmetric device, assembled by the β-Ta
2
O
5
/SWCNTs and activated carbon films, shows a high energy density of 45.5 Wh kg
−1
at the power density of 10.8 kW kg
−1
. This technique opens up a new avenue for improving the energy density and rate performance of flexible supercapacitors.
•The rapid degradation of methylene blue within 150 min kept the material clean.•Enhanced filtration efficiency by forming an uneven nanofiber structure.•Exceptional antibacterial properties against ...E. coli and S. aureus.•Nanofibrous membranes were found to affect the replication and transcription of SARS-CoV-2 virus by molecular docking.•Nanofiber membranes exhibited antiviral activity against H3N2 virus.
Public health safety issues have been plaguing the world since the pandemic outbreak of coronavirus disease (COVID-19). However, most personal protective equipments (PPE) do not have antibacterial and anti- toxicity effects. In this work, we designed and prepared a reusable, antibacterial and anti-toxicity Polyacrylonitrile (PAN) based nanofibrous membrane cooperatedwith Ag/g-C3N4 (Ag-CN), Myoporum.bontioides (M. bontioides) plant extracts and Ag nanoparticles (NPs) by an electrospinning-process. The SEM and TEM characterization revealed the formation of raised, creased or wrinkled areas on the fiber surface caused by the Ag nanoparticles, the rough surface prevented the aerosol particles on the fiber surface from sliding and stagnating, thus providing excellent filtration performance. The PAN/M. bontioides/Ag-CN/Ag nanofibrous membrane could be employed as a photocatalytic bactericidal material, which not only degraded 96.37% of methylene blue within 150 min, but also exhibited the superior bactericidal effect of 98.65 ± 1.49% and 97.8 ± 1.27% against E. coli and S. aureus, respectively, under 3 hs of light exposure. After 3 cycles of sterilization experiments, the PAN/M. bontioides/Ag-CN/Ag nanofibrous membrane maintained an efficient sterilization effect. Molecular docking revealed that the compounds in M. bontioides extracts interacted with neo-coronavirus targets mainly on Mpro and RdRp proteins, and these compounds had the strongest docking energy with Mpro protein, the shortest docking radius, and more binding sites for key amino acids around the viral protein targets, which influenced the replication and transcription process of neo-coronavirus. The PAN/M.bontioides/Ag-CN/Ag nanofibrous membrane also performed significant inhibition of influenza A virus H3N2. The novel nanofiber membrane is expected to be applied to medical masks, which will improve human isolation and protection against viruses.
Electrocardiography (ECG) is vital in diagnosing and preventing cardiovascular diseases (CVDs), which is the third leading cause of global mortality. Real-time ECG monitoring helps understand ...athletes' physical conditions during training, thereby optimizing their performance. However, the accuracy of ECG signals from traditional ECG devices can be influenced by various factors, notably body movement, which can significantly disturb the electrode-skin impedance and the signal quality. Consequently, there is a pressing demand for portable, compact, and biocompatible real-time ECG monitoring devices with low noise level. The creation of flexible, skin-adhesive, and anti-motion interference electrodes, coupled with advancements in post noise reduction algorithms, has emerged as effective strategies. This review commences with an overview of typical ECG electrodes, followed by an exploration of recent developments in dry electrodes, which demonstrate considerable potential for anti-motion interference monitoring. Then, the prevalent circuits and modules used for signal acquisition and processing are discussed. Additionally, we delve into the causes of signal noise and effective methods of noise reduction. Lastly, we address future challenges and research directions in the field of anti-motion interference ECG monitoring.
Smart electromagnetic interference (EMI) shielding materials with dynamic adjustable shielding performances are attractive, which however still suffer from complicated preparation, large thickness, ...inconvenient trigger mode, and relatively narrow adjustment range. Here, the off/on switchable EMI shielding elastomers are developed
via
precisely dispersing the spiked Ni microparticles (diameter: 2–3 µm) into polydimethylsiloxane matrix. By the gentle stir under low speed (300 r min
−1
) for a short time (3 min), the Ni particles could form irregular clusters as short chains, which approach being linked together but still maintain tiny gaps (<3 µm) among them, showing a unique pre-linked-chains arrangement. The original elastomers show good impedance matching and low dielectric loss, allowing most EM waves to pass through. The stretching force presses the short pre-linked Ni chains to form massive microscale conductive paths inside (enlarge ∼6 orders of the original conductivity at 20% strain), which significantly enhance the capability of conduction loss and therefore trigger strong EMI shielding ability. The elastomer exhibits continuously and reversibly variable EMI shielding performances (∼35 dB for a 0.3 mm single film, ∼55 dB for the sandwich design) during mechanical stretching and releasing by 0–20% strain. In addition, the applicability of the smart EMI shielding elastomer is demonstrated by a wireless strain sensing system, which shows the possibility for the wearable smart EMI shielding to monitor human body motion precisely.
Clostridioides difficile
sequence type 2 (ST2) has been increasingly recognized as one of the major genotypes in China, while the genomic characteristics and biological phenotypes of Chinese ST2 ...strains remain to be determined. We used whole-genome sequencing and phylogenetic analysis to investigate the genomic features of 182 ST2 strains, isolated between 2011 and 2017. PCR ribotyping (RT) was performed, and antibiotic resistance, toxin concentration, and sporulation capacity were measured. The core genome Maximum-likelihood phylogenetic analysis showed that ST2 strains were distinctly segregated into two genetically diverse lineages L1 (67.0% from Northern America) and L2, while L2 further divided into two sub-lineages, SL2a and SL2b (73.5% from China). The 36 virulence-related genes were widely distributed in ST2 genomes, but in which only 11 antibiotic resistance-associated genes were dispersedly found. Among the 25 SL2b sequenced isolates, RT014 (40.0%,
n
= 10) and RT020 (28.0%,
n
= 7) were two main genotypes with no significant difference on antibiotic resistance (χ
2
= 0.024–2.667,
P
> 0.05). A non-synonymous amino acid substitution was found in
tcdB
(Y1975D) which was specific to SL2b. Although there was no significant difference in sporulation capacity between the two lineages, the average toxin B concentration (5.11 ± 3.20 ng/μL) in SL2b was significantly lower in comparison to those in L1 (10.49 ± 15.82 ng/μL) and SL2a (13.92 ± 2.39 ng/μL) (χ
2
= 12.30,
P
< 0.05). This study described the genomic characteristics of
C. difficile
ST2, with many virulence loci and few antibiotic resistance elements. The Chinese ST2 strains with the mutation in codon 1975 of the
tcdB
gene clustering in SL2b circulating in China express low toxin B, which may be associated with mild or moderate
C. difficile
infection.
Severe fever with thrombocytopenia syndrome bunyavirus is a newly discovered bunyavirus with high pathogenicity to human. The transmission model has been largely uncharacterized. Investigation on a ...cluster of severe fever with thrombocytopenia syndrome cases provided evidence of person-to-person transmission through blood contact to the index patient with high serum virus load.
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•The multi-level superimposed strategy is proposed to enhance EMI SE of Ti3C2 film.•The increment in the gap distance results in the enhancement of EMI SE.•The resonance induces a ...multiple-wave interference in a certain gap distance.•Ti3C2 coating has the commercial advantages in blocking the EM radiation.
The progressive development of next-generation flexible and portable electronics highly requires electromagnetic interference (EMI) shielding layers with a trade-off among high shielding effectiveness (SE), lightweight and flexible nature. Herein, a multiscale layered structure optimization strategy is proposed to enhance EMI shielding performance through orderly superimposing individual free-standing Ti3C2 (F-Ti3C2) film with an ultrathin thickness of ∼ 4 μm. Compared to a bulk F-Ti3C2 film (64.6 dB) with the same weight of 150 mg, the multi-level superimposed Ti3C2 films with six individual layers (S-Ti3C2-6) show much higher EMI shielding performance (84.7 dB). Furthermore, the increment in the gap distance between the adjacent F-Ti3C2 films can also increase the EMI SE from 51.1 dB (d = 0 mm) to 74.9 dB (d = 1.5 mm). The underlying mechanism can be attributed to the synergistic effect of multiple internal reflections between Ti3C2 nanosheets and multiple-wave interference between adjacent F-Ti3C2 films. Furthermore, the multi-layered design strategy can be extended to other EMI materials including polypyrrole and graphite, etc. The demonstration of Ti3C2 coatings to block EM waves proves their considerable advantages over commercial shielding packages. This work provides a novel methodology to design EMI shielding layers for the practical applications.
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