Paraoxonase 1 (PON1) is the enzyme that removes carcinogenic radicals from lipids. The aim of the study was to investigate the differences in PON1 activity and oxidation stress parameters between ...patients with cervical intraepithelial neoplasia (CIN) and healthy controls.
The study included 65 women with CIN and 109 healthy women. Lipid parameters were determined on Cobas Integra 400 plus (Roche, Mannheim, Germany). Tiols and reduced glutathione (GSH) were determined spectrophotometric using Eliman reagent. Activity of PON1 was assessed with two substrates, paraoxon and phenylacetate by spectrophotometric method. Malondialdehyde (MDA) was determined by high performance liquid chromatography (Shimadzu Corporation, Kyoto, Japan). Mann-Whitney-test, t-test, χ2-test, correlation and logistic regression was used in statistical analysis. P < 0.05 was considered statistically significant.
The basal (P = 0.929) and NaCl-stimulated (P = 0.985) PON1 activity and activities standardised on the concentration of high-density lipoprotein (HDL; P = 0.076; P = 0.065, respectively) and apolipoprotein AI (apo AI; P = 0.444; P = 0.499, respectively) as well as PON1 phenotypes (P = 0.842) did not differ significantly between the groups. The PON1 arylesterase activity (53±19 kU/L vs. 77±17 kU/L; P < 0.001) and HDL-standardized activity (37 (28-44) kU/mmol
. 43 (37-50) kU/mmol; P < 0.001) and apoAI (29±11 kU/g
. 44±11 kU/g; P < 0.001) was significantly reduced in the CIN group. The concentration of the thiol groups was similar (P = 0.519), of MDA was lower (0.39 (0.27-0.55) µmol/L
. 0.76 (0.57-1.15) µmol/L; P < 0.001) and of GSH was higher (112.0 (66.0-129.6) µg/mL
. 53.4 (34.8-134.4) µg/mL; P < 0.001) in the CIN group.
Reduced PON1 arylesterase activity, lower MDA and higher GSH concentration were observed in CIN patients.
Background and purpose: Prunus spinose L. is a traditionally consumed, recently scientifically reexamined plant. Brain bioavailability and functionality of polyphenols (PPH) of blackthorn flower ...extract (PSE) was investigated.Materials and methods: C57BL/6 mice received oral daily repeated doses of 25 mg/kg body weight of total PSE polyphenols for 28 days. Brain concentrations of individual polyphenols from PSE were determined by UPLC/MS on 1st,7th, 14th, 21st, and 28th day. Brain antioxidative defense markers were examined as indicators of functionality after bioaccumulation.Results: A total of 68.7% PPH present in PSE were detected in the brain. Higher (p≤0.05) Cmax/AUClast in the PSE treatment vs. control group was recorded for 59.1% of brain detected compounds, indicating relatively good bioaccumulation in the brain. The highest present compounds in PSE were not necessarily the ones mostly bioapsorbed in the brain. Kaempherols were not significantly distributed, opposite to phenolic acids, quercetins or epicgaloatechin-3-gallate. The compounds with the highest concentrations on 28th day were 4-p-coumaroylquinonic acid, (-)-epicatechin, quercetin-3-O-rutinoside, quercetin–rhamnoside, kaempherol-3-rutinoside and quercetin-3-gucoside. Brain lipid peroxidation (MDA) decreased (p<0.05) on the 21st and 28th day in the PSE group. Increase (p<0.05) in GSH concentration was observed on the 21st and 28th and SOD activity on the 28th day. Catalase activity was unchanged. It could be hypothesized that highest PPH concentration-ratios, caused reduction of lipid peroxidation by radical scavenging and simultaneous induction of glutathione and SOD pathways.Conclusions: Screened compounds could be candidates for examining or creation of brain targeted “neuro-nutriceuticals” polyphenol mixtures.
The 20-year experience with large loop excision of the transformation zone (LLETZ) at Gynecologic Oncology Unit, Department of Gynecology and Obstetrics, Sestre milosrdnice University Hospital Centre ...from Zagreb is presented. This retrospective observational study included 1407 women with cervical dysplasia treated by LLETZ technique during the 1995-2016 period. LLETZ was most commonly performed in the 25-35 age group (51%), followed by the 36-45 age group (22%), and least frequently in the >65 age group (2%). Histopathologic results lower than high-grade squamous intraepithelial lesion were found in 23% and high grade squamous intraepithelial lesion or worse findings in 77% of patients. Positive margin as a sign of possible residual dysplasia was found in 25% of cones, 80% of which included endocervical positive margin. Cervical canal biopsy result was positive in 18% of cases. Accurate colposcopy and its findings can help avoid overtreatment, the rate of which was higher than expected in our retrospective study. Long-term follow up is an imperative for proper assessment of the procedure success. This method is the best choice for complete disease removal without unnecessary overtreatment, but it requires continuous education and training of the whole team.
Hemiperitoneum caused by venous bleeding from the hilum of the spleen is a rare cause of acute abdomen in the last trimester of pregnancy.
We are presenting a case of a twenty-nine-year-old primipara ...with the clinical picture of acute abdomen.
Primipara in the 36th week of pregnancy presented with the clinical picture of acute abdomen with the ultrasound finding of free fluid in the abdominal cavity and foetal bradycardia. Considering the clinical picture of the mother and the risks for the foetus, it was decided to complete the pregnancy with an emergency caesarean section. During the procedure, exploration of the abdominal cavity found the bleeding site from the venae gastricae breves, and a surgeon stopped active bleeding.
Non-obstetric bleedings are not common in pregnancy, but they are life-threatening both to the mother and the foetus. The exact cause is usually found during the surgical procedure. If a pregnant woman presents with a clinical picture of abdominal pain, and the signs of foetal distress or clinical instability of the mother are also present, an emergency surgical procedure is indicated. In this case, the indication was foetal bradycardia, and the child was born alive by a caesarean section, while an extended surgical procedure saved the mother's life.
SAŽETAK
UvodPrimarna prevencija raka vrata maternice uključuje informiranje i poticanje svijesti o odgovornom spolnom ponašanju i metodama prevencije spolno prenosivih infekcija i kontracepcije. U ...okviru mjera primarne prevencije, cijepljenje protiv humanog papilomavirusa (HPV) jest dostupna i učinkovita dopuna mjerama zaštite od premalignih i malignih lezija anogenitalne regije. Cilj rada bio je istražiti znanje, mišljenja, stavove o cijepljenju i status cijepljenja liječnika protiv HPV-a u Hrvatskoj.
MetodeDeskriptivna, opažajna, presječna studija provedena je među članovima Hrvatske liječničke komore koristeći elektronički upitnik. Analizirana su sociodemografska obilježja ispitanika, znanja, mišljenja i stavovi o cijepljenju, status cijepljenja i spremnost na cijepljenje ispitanika i njihove djece. Analiza rezultata provedena je hi-kvadrat testom.
RezultatiU analiziranoj skupini (N=1,688 od 18,421) ispitanika, zagovarateljima/cama cijepljenja smatra se 91,59% (N=1546), protivnicima 3,14% (N=53), a 5,27% (N=89) nema stav prema cijepljenju. Udio liječnika cijepljenih protiv HPV-a iznosi 12,32% (N=208), 19,73% (N=333) je cijepilo dijete, 46,64% (N=632 od 1355) onih koji nisu cijepili ili nemaju djecu, planira to učiniti. Najveću skupinu cijepljenih liječnika čine liječnici bez specijalizacije (n=112 od 371, 30,19%), mlađe dobne skupine. Većina liječnika (99,17%, N=1674) smatra da je cjepivo protiv HPV-a dostupno u Republici Hrvatskoj, 86,85% (N=1466) smatra da je cijepljenje namijenjeno osobama oba spola starijima od 9 godina, 60,55% ispitanika (N=1022) smatra da cervikalna displazija ne predstavlja kontraindikaciju za cijepljenje. Kao najčešći razlozi protivljenja cijepljenju protiv HPV-a navedene su nuspojave i mišljenje da je cijepljenje nepotrebno.
ZaključakVećina ispitanika ovog istraživanja ima pozitivan stav o cijepljenju protiv HPV-a. Iako je stopa liječnika i njihove djece cijepljenih protiv HPV-a razmjerno niska, većina ispitanika spremna je cijepiti svoje dijete. Niska stopa cijepljenih liječnika vjerojatno je posljedica manjeg broja ispitanika mlađih dobnih skupina koji su imali priliku biti cijepljeni. Ipak, spremnost na cijepljenje djece važan je pokazatelj stava o cijepljenju.
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