Bee pollen is recommended as dietary supplement due to immunostimulating functions including antioxidant, anti-inflammatory and anti-carcinogenic properties. Nevertheless, the effectiveness of such ...properties is still not well understood. As diet can be associated with animal performance, microbiota modulation and potentially factor for cancer, this study aimed to analyze if bee pollen could influence growth, gut microbial and skin cutaneous melanoma development in zebrafish. Control diets based on commercial flakes and Artemia were compared with the same diet supplemented with bee pollen. Fish weight gain, increased length, intestinal bacteria metagenomics analysis, serum amyloid A gene expression and cutaneous melanoma transplantation assays were performed. Bee pollen affected microbiota composition and melanoma development. Differential abundance revealed higher abundance in the control group for Aeromonadaceae family, Aeromonas and Pseudomonas genus, A. sobria, A. schubertii, A. jandaei and P. alcaligenes species compared with pollen diet group. Pollen group presented higher abundance for Chromobacterium genus and for Gemmobacter aquaticus, Flavobacterium succinicans and Bifidobacterium breve compared with control group. Unexpectedly, fish fed with bee pollen showed higher tumor growth rate and larger tumor size than control group. This is the first study to report intestinal microbial changes and no protective cancer properties after bee pollen administration.
This study describes the development of acetylcholinesterase (AChE), carboxylesterases (CaE1, CaE2, CaE3), glutathion-S-transferase (GST), alkaline phosphatase (ALP) and catalase (CAT) as enzyme ...biomarkers of exposure to xenobiotics such as thiamethoxam in the honey bee Apis mellifera. Extraction efficiency, stability under freezing and biological variability were studied. The extraction procedure achieved good recovery rates in one extraction step and ranged from 65 percent (AChE) to 97.3 percent (GST). Most of the enzymes were stable at −20°C, except ALP that displayed a slight but progressive decrease in its activity. Modifications of enzyme activities were considered after exposure to thiamethoxam at the lethal dose 50 percent (LD50, 51.16ngbee−1) and two sublethal doses, LD50/10 (5.12ngbee−1) and LD50/20 (2.56ngbee−1). The biomarker responses revealed that, even at the lowest dose used, exposure to thiamethoxam elicited sublethal effects and modified the activity of CaEs, GST, CAT and ALP. Different patterns of biomarker responses were observed: no response for AChE, an increase for GST and CAT, and differential effects for CaEs isoforms with a decrease in CaE1 and CaE3 and an increase in CaE2. ALP and CaE3 displayed contrasting variations but only at 2.56ngbee−1. We consider that this profile of biomarker variation could represent a useful fingerprint to characterise exposure to thiamethoxam in the honey bee A. mellifera. This battery of honey bee biomarkers might be a promising option to biomonitor the health of aerial and terrestrial ecosystems and to generate valuable information on the modes of action of pesticides.
► Development of biomarkers of exposure to thiamethoxam in the honey bee. ► Thiamethoxam modulates the activity of seven enzymes. ► Differential biomarker responses provide a fingerprint of exposure to thiamethoxam.
Bee pollen, a natural resource collected by bees, is rich in many nutrients, therefore it may represent a useful dietary supplement. Different uses of bee pollen are proposed due to its beneficial ...health properties, which includes the capacity to improve animal performance and promote immunostimulation. Animal nutrition can directly affect adults and their offspring, and larval stage is a critical moment for fish due to high mortality related to immune challenges. Thus, the present study attempted to evaluate the effects of adding bee pollen to a zebrafish diet, specifically, analyzing the effects on reproduction and immunity transference to descendants. Zebrafish adults received control diets based on commercial flakes and live food Artemia sp. nauplii or bee pollen-supplemented diets, administered three times a day, at the same time. The animals received the diets over 60 d, and throughout this period, they were tested for: egg production per female, total number of eggs, embryo viability rate, larval survival rate after exposure to spring viremia of carp virus and to Salmonella enterica serovar Typhimurium, and larval neutrophil recruitment after tail wounding. Bee pollen supplementation failed to improve egg production and embryo viability, and was unable to substitute flakes in zebrafish breeders. Instead, the offspring of breeders fed with bee pollen supplemented diets showed longer survival upon virus exposure and higher neutrophil migration to wounds. These results indicate that bee pollen can influence vertical immunity through important mechanisms related to offspring immunity in the early stages, when larval immune system is not fully developed.
•Bee pollen in the diet did not alter zebrafish egg and embryo viability.•Bee pollen influenced the vertical immunity of zebrafish.•Bee pollen improved larval resistance to viral infection and neutrophil migration.•Bee pollen did not alter larval resistance to bacterial infection.
Fipronil is a neurotoxic insecticide that inhibits the gamma-aminobutyric acid receptor and can affect gustative perception, olfactory learning, and motor activity of the honeybee
Apis mellifera
. ...This study determined the lethal dose (LD
50
) and the lethal concentration (LC
50
) for Africanized honeybee and evaluated the toxicity of a sublethal dose of fipronil on neuron metabolic activity by way of histochemical analysis using cytochrome oxidase detection in brains from worker bees of different ages. In addition, the present study investigated the recovery mechanism by discontinuing the oral exposure to fipronil. The results showed that mushroom bodies of aged Africanized honeybees are affected by fipronil, which causes changes in metabolism by increasing the respiratory activity of mitochondria. In antennal lobes, the sublethal dose of fipronil did not cause an increase in metabolic activity. The recovery experiments showed that discontinued exposure to a diet contaminated with fipronil did not lead to recovery of neural activity. Our results show that even at very low concentrations, fipronil is harmful to honeybees and can induce several types of injuries to honeybee physiology.
ABSTRACT The use of insecticides is common in pest management in wheat crops but may lead to higher production costs and environmental contamination. Therefore, a more comprehensive and uniform ...distribution of plant protection products in the desired target is recommended. The objective of this study is to evaluate the effect of different spray nozzles, application rates, and adjuvants on droplet spectrum and spray deposition in wheat crops. The physicochemical characteristics of the spray mixture, droplet spectrum, and spray deposition in wheat plants were evaluated. The lower application rate (75 L ha−1) combined with the use of the twin flat fan tip AS7030 and the adjuvant sodium lauryl ether sulfate (SLES) yielded droplets with a larger diameter. However, insecticide spraying using hollow cone tip MGA 015 without adjuvants provided the highest percentage of droplets susceptible to drift. SLES significantly decreased the percentage of drift by increasing droplet size, and the adjuvant silicone, d-limonene, and polyester copolymer increased spray deposition. The evaluated adjuvants increased the electrical conductivity and reduced the surface tension of the spray mixtures. These results indicate that it is possible to reduce the application rate from 150 to 75 L ha−1 without affecting spray deposition on wheat leaves.
ABSTRACT We analyze the distribution of arrival directions of ultra-high-energy cosmic rays recorded at the Pierre Auger Observatory in 10 years of operation. The data set, about three times larger ...than that used in earlier studies, includes arrival directions with zenith angles up to 80°, thus covering from to in declination. After updating the fraction of events correlating with the active galactic nuclei (AGNs) in the Véron-Cetty and Véron catalog, we subject the arrival directions of the data with energies in excess of 40 EeV to different tests for anisotropy. We search for localized excess fluxes, self-clustering of event directions at angular scales up to 30°, and different threshold energies between 40 and 80 EeV. We then look for correlations of cosmic rays with celestial structures both in the Galaxy (the Galactic Center and Galactic Plane) and in the local universe (the Super-Galactic Plane). We also examine their correlation with different populations of nearby extragalactic objects: galaxies in the 2MRS catalog, AGNs detected by Swift-BAT, radio galaxies with jets, and the Centaurus A (Cen A) galaxy. None of the tests show statistically significant evidence of anisotropy. The strongest departures from isotropy (post-trial probability %) are obtained for cosmic rays with EeV in rather large windows around Swift AGNs closer than 130 Mpc and brighter than 1044 erg s−1 (18° radius), and around the direction of Cen A (15° radius).
When houseflies find optimal conditions to develop, they rapidly increase their population size negatively impacting both humans and animals through nuisance and the transmission of pathogens. Musca ...domestica Linnaeus, 1758 (Diptera: Muscidae) shows a preference for animal faeces and for this reason it is a serious pest in animal breeding facilities. To prevent proliferation of houseflies and to evaluate the efficiency of the control methods, it is important to routinely monitor the activity of this dipteran in such facilities. There are several types of traps for sampling houseflies in breeding sites, here we used colored pan-traps to evaluate the efficiency of the trap and its color in sampling M. domestica in a cage poultry facility located in the city of Nepomuceno, Minas Gerais, Brazil. To do so, we set up yellow, white, blue and red pan-traps next to the cages of poultries and collected flies once a week for a period of a year. Although the density of M. domestica was high during the entire period of sampling, more flies were collected in white (monthly average of 470.83 flies) than in yellow (327.55), blue (267.14) and red traps (199.63). Hence, we argue that this poultry farm needs to improve its housefly monitoring program and we suggest a continuous use of white pan-traps to monitor the efficiency of the pest control methods implemented in the facility.
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