Geoffroy's cat (Leopardus geoffroyi) is a small Neotropical felid whose social behavior remains poorly understood. We used simultaneous radiotelemetry (4 males and 3 females) and camera trapping to ...examine the spatial structure and dynamics of a population of this species in the Brazilian pampas (part of the Uruguayan Savannah ecoregion), including assessment of interindividual genetic relatedness. Home ranges (HRs) and core areas of males were larger than those of females. Males were significantly heavier than females, and linear regression analyses indicated that body mass of males significantly influenced HR size, whereas the relationship was not significant for females. When we performed a joint analysis comparing our data to those reported previously for other study sites, we observed the same patterns. We detected extensive HR and core area overlap among most of our monitored individuals, with no clear sex-based pattern. Moreover, our molecular data indicated that most of the sampled individuals were unrelated, and that the levels of HR and core area overlap were not significantly influenced by genetic relatedness. These results suggest that some interindividual tolerance and relaxation in territoriality may occur in Geoffroy's cat. We detected only minor changes in HR and core area size and overlap between seasons, indicating that the spatial structure we observed was temporally stable. On a broader perspective, our cross-site comparisons illustrate the usefulness of performing multiple ecological studies employing comparable methods at different locations to better understand the ecology of wild felid populations.
The Neotropical region currently harbors 8 recognized species of small cats, including the margay (Leopardus wiedii) and jaguarundi (Puma yagouaroundi). These similar-sized species have broad ...geographic distributions and commonly occur in sympatry. We constructed species occurrence databases and used the maximum entropy (Maxent) modeling approach to develop models of habitat suitability for each of these species. We identified the environmental predictors with the highest importance for species occurrence and evaluated how they influence habitat suitability for both wild cats. Variables related to temperature and precipitation were good predictors of species occurrence, with both cats responding similarly to them. However, margay showed higher variation in habitat suitability depending on local climatic predictors. The estimated models indicated high niche similarity (> 76%) between these cats, but despite this we identified different areas with the most favorable conditions for each species' occurrence. Approximately 50% and 72% of the entire suitable area were estimated as being moderately to highly suitable for margay and jaguarundi, respectively, with the margay model comprising a higher proportion of forest environments in comparison to that of the jaguarundi. These findings will be relevant in the context of conservation planning when indicating the priority areas for their long-term preservation.
BRAZIL ROAD-KILL Grilo, Clara; Coimbra, Michely R.; Cerqueira, Rafaela C. ...
Ecology (Durham),
11/2018, Volume:
99, Issue:
11
Journal Article
Peer reviewed
Open access
Mortality from collision with vehicles is the most visible impact of road traffic on wildlife. Mortality due to roads (hereafter road-kill) can affect the dynamic of populations of many species and ...can, therefore, increase the risk of local decline or extinction. This is especially true in Brazil, where plans for road network upgrading and expansion overlaps biodiversity hotspot areas, which are of high importance for global conservation. Researchers, conservationists and road planners face the challenge to define a national strategy for road mitigation and wildlife conservation. The main goal of this dataset is a compilation of geo-referenced road-kill data from published and unpublished road surveys. This is the first Data Paper in the BRAZIL series (see ATLANTIC, NEOTROPICAL, and BRAZIL collections of Data Papers published in Ecology), which aims make public road-kill data for species in the Brazilian Regions. The dataset encompasses road-kill records from 45 personal communications and 26 studies published in peer-reviewed journals, theses and reports. The road-kill dataset comprises 21,512 records, 83% of which are identified to the species level (n = 450 species). The dataset includes records of 31 amphibian species, 90 reptile species, 229 bird species, and 99 mammal species. One species is classified as Endangered, eight as Vulnerable and twelve as Near Threatened. The species with the highest number of records are: Didelphis albiventris (n = 1,549), Volatinia jacarina (n = 1,238), Cerdocyon thous (n = 1,135), Helicops infrataeniatus (n = 802), and Rhinella icterica (n = 692). Most of the records came from southern Brazil. However, observations of the road-kill incidence for non-Least Concern species are more spread across the country. This dataset can be used to identify which taxa seems to be vulnerable to traffic, analyze temporal and spatial patterns of road-kill at local, regional and national scales and also used to understand the effects of road-kill on population persistence. It may also contribute to studies that aims to understand the influence of landscape and environmental influences on road-kills, improve our knowledge on road-related strategies on biodiversity conservation and be used as complementary information on large-scale and macroecological studies. No copyright or proprietary restrictions are associated with the use of this data set other than citation of this Data Paper.
Felids are among the species most threatened by habitat fragmentation resulting from land-use change. In the Uruguayan Savanna ecoregion, about 30% of natural habitats have been lost, large felids ...have been eradicated from most of the region, and the impact of anthropogenic threats over the smaller species that remain is unknown. To develop management strategies, it is important to enhance knowledge about species population structure and landscape connectivity, particularly when land-use change will continue and intensify in the next years. In this study, we evaluate the population structure and gene flow of Geoffroy's cat in the Uruguayan Savanna ecoregion. We generated a matrix of 11 microsatellite loci for 70 individuals. Based on Bayesian approaches we found that within the Uruguayan Savanna, Geoffroy's cat shows high levels of genetic variability and no population structure. However, we observed genetic differences between individuals from the Uruguayan Savanna and those from the contiguous ecoregion, the Argentinian Humid Pampa. Four first-generation migrants from Humid Pampa were identified in the Uruguayan Savanna, suggesting a stronger gene flow in the west-east direction. We detected a past bottleneck followed by a subsequent recovery in Geoffroy's cat populations in both ecoregions. These results lay the groundwork to understand the population dynamics and conservation status of Geoffroy's cat in the Uruguayan Savanna ecoregion, and provide baseline data to establish population monitoring.
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•Higher habitat suitability in the central area of the species’ distribution.•Demographic estimates indicated small population & threatened conservation status.•Very small percentage ...of highly suitable habitat is protected.•Most suitable patches are poorly connected.•Crucial connectivity paths are likely to be lost in future.
Muñoa’s pampas cat (recently proposed to be a distinct species, Leopardus munoai) is a small felid that is endemic to the Uruguayan Savanna ecoregion (encompassing southern Brazil, north-eastern Argentina and Uruguay). Previous studies have suggested that it is threatened, but its conservation assessment has been hampered by the scarcity of data on its ecology, including spatial distribution, population size, and connectivity. To address these issues, we developed current spatial distribution models and used them to: (i) identify the environmental variables affecting L. munoai habitat suitability; (ii) generate estimates of population size to assess its conservation status based on IUCN criteria; (iii) estimate habitat suitability in protected areas; (iv) identify potential paths of connectivity among protected areas and sites of confirmed occurrence; and (v) assess the proportion of the estimated connectivity paths that overlap with threatened areas (based on future threat projections). Our results indicated higher habitat suitability in the central area of the species’ distribution. All estimates (based on different demographic assumptions) indicated that L. munoai should be categorized in one of the IUCN threatened categories. Worryingly, several estimates indicated that it may be Critically Endangered. Only 0.73 % of its high-suitability landscape is presently protected, and connectivity among most protected areas and occurrence records was low. Additionally, areas with estimated connectivity among occurrence records mostly overlapped with regions with a high level of future habitat loss threat (92.46 %), highlighting the urgent need for an international approach to ensure the long-term survival of this elusive felid.
This work has as objective to analyze the recycling possible uses of the slag of welding flux (SWF) in the civil construction. It was searched the liability of SWF as substitute of sand in the ...production of multiple-use mortars and clay for the production of ceramic bricks. SWF has origin in the submerged-arc welding (SAW), and have been used slags of the acid, neutral and basic types, according to its neutrality. Mortars have been made containing the three types of SWF and in addition a reference mortar made with sand. Physical and mineralogical characteristics of each SWF were studied, and the physical properties (consistency and density) and mechanical properties (compressive and tensile strengths) of the mortars were analyzed. Ceramic pieces have been made containing kaolinitic sedimentary clay up to 10 wt. % of SWF. The pieces were prepared by uniaxial pressing and fired at 950ºC. The following properties after firing were determined: linear shrinkage, water absorption, apparent porosity, apparent density, and flexural strength. The results show that for multiple-use mortars, SWF reached excellent results, being an overall substitute for sand as fine aggregate. For ceramic bricks, SWF can also be used as partial substitute for red clay. Both applications of SWF significantly contributes for the reduction of the ambient impact: on one hand, reducing the extraction of natural sand and clay, on other hand, contributing for the reduction of the risk of ground water contamination due to the inadequate disposal of the SWF.
Este trabalho tem como objetivo avaliar possíveis usos para a reciclagem da escória de fluxo de soldagem (EFS) na construção civil. Foi pesquisada a possibilidade do uso da EFS como substituto da areia na produção de argamassas de múltiplo-uso e substituição da argila na produção de tijolos. A EFS tem origem na soldagem a arco submerso (SAS), e foram usadas escórias dos tipos ácida, neutra e básica, conforme sua neutralidade. As argamassas foram preparadas usando os três tipos de EFS e ainda uma argamassa de referência feita com areia. As características físicas e mineralógicas de cada EFS foram estudadas, e as propriedades físicas (consistência e densidade) e propriedades mecânicas (resistência à compressão e à tração) das argamassas foram analisadas. Corpos de prova cerâmicos foram preparados contendo argila caulinítica sedimentar com até 10% em peso de EFS. Estes foram confeccionados por pressão uniaxial e calcinados a 950ºC. As seguintes propriedades após a queima foram determinadas: deformação linear, absorção de água, densidade aparente e resistência à flexão. Os resultados mostraram que para as argamassas de múltiplo-uso, a EFS atingiu resultados excelentes, podendo substituir totalmente a areia como agregado miúdo. Para os tijolos cerâmicos, a EFS também pode ser usada como substituto parcial da argila vermelha. Ambas as aplicações da EFS contribuem significativamente para a redução de impacto ambiental: por um lado, reduzindo a extração de areia natural e da argila e, por outro lado, contribuindo para a redução do risco de contaminação do subsolo devido ao despejo inadequado da EFS.
BRAZIL ROAD-KILL Grilo, Clara; Coimbra, Michely R.; Cerqueira, Rafaela C. ...
Bulletin of the Ecological Society of America,
01/2019, Volume:
100, Issue:
1
Journal Article
Ro52 is a member of the TRIM family of single-protein E3 ligases and is also a target for autoantibody production in systemic lupus erythematosus and Sjögren's syndrome. We previously demonstrated a ...novel function of Ro52 in the ubiquitination and proteasomal degradation of IRF3 following TLR3/4 stimulation. We now present evidence that Ro52 has a similar role in regulating the stability and activity of IRF7. Endogenous immunoprecipitation of Ro52-bound proteins revealed that IRF7 associates with Ro52, an effect which increases following TLR7 and TLR9 stimulation, suggesting that Ro52 interacts with IRF7 post-pathogen recognition. Furthermore, we show that Ro52 ubiquitinates IRF7 in a dose-dependent manner, resulting in a decrease in total IRF7 expression and a subsequent decrease in IFN-alpha production. IRF7 stability was increased in bone marrow-derived macrophages from Ro52-deficient mice stimulated with imiquimod or CpG-B, consistent with a role for Ro52 in the negative regulation of IRF7 signalling. Taken together, these results suggest that Ro52-mediated ubiquitination promotes the degradation of IRF7 following TLR7 and TLR9 stimulation. As Ro52 is known to be IFN-inducible, this system constitutes a negative-feedback loop that acts to protect the host from the prolonged activation of the immune response.
The reduction in pH from atmospheric inputs of CO2 (ocean acidification, OA) threatens marine calcifiers, including the eastern oyster (Crassostrea virginica), that precipitate biogenic CaCO3 for ...shell formation. Recent investigations have demonstrated that alterations in gene expression enable bivalves to respond to episodic low pH. Evidence generated from several studies highlighted the importance of upregulating genes related to biomineralization, ion transport, and acid-base balance such as carbonic anhydrase (CA) genes. Two experiments were designed to evaluate the effect of acidification on calcification processes and to probe the specific role of CA in oyster resilience to low pH. First, adult oysters were exposed to eight months of chronic acidification stress (pH ∼7.3, pCO2 ∼3300 ppm) or control conditions (pH ∼7.9, pCO2 ∼500 ppm) before shells were artificially damaged and shell repair monitored. Results showed a dramatic decrease in shell regeneration after chronic high pCO2 exposure (only 30% of oysters regrew any shell) suggesting that mechanisms that promote calcification under high pCO2 conditions may not be sustainable for extended periods of time. To further explore these mechanisms, a second experiment was designed by focusing on the role of CA in mitigating acidification stress. Here, adult oysters received an injection of acetazolamide in dimethyl sulfoxide (DMSO) to inhibit CA or DMSO (control) before rearing in control (pH ∼8.1, pCO2 ∼340 ppm) or acidified (pH ∼7.3, pCO2 ∼3300 ppm) conditions. After three weeks, oyster shells were damaged and shell repair monitored. Oysters incubated at low pH seawater with CA inhibition had the least amount of shell regeneration at the end of 21-day regrowth period. Interestingly, oysters were able to increase intracellular pH (pHi) of hemocytes under low pH conditions; however, this ability was significantly diminished with CA inhibition. Results highlight the role of CA in maintaining calcification under low pH conditions by establishing an intracellular environment favorable to calcium carbonate precipitation.
•Shell repair dramatically decreased in oysters after chronic high pCO2 exposure.•Carbonic anhydrase (CA) inhibition further reduces shell repair.•Oysters increased hemocyte intracellular pH under low pH seawater.•Ability to increase intracellular pH of hemocytes was lost when CA was inhibited.•CA plays a role in maintaining calcification under low pH.
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