The level of stem cell proliferation must be tightly controlled for proper development and tissue homeostasis. Multiple levels of gene regulation are often employed to regulate stem cell ...proliferation to ensure that the amount of proliferation is aligned with the needs of the tissue. I focus on proteasome-mediated protein degradation as a means of regulating the activities of proteins involved in controlling the stem cell proliferative fate in the C. elegans germline. Previously, RFP-1 (RING finger protein), a putative E3 ubiquitin ligase was identified as being involved in regulating the proliferative fate. I demonstrated that RFP-1 binds to and causes proteasome-mediated degradation of MRG-1 (mortality related gene), a homologue of the mammalian chromodomain-containing protein MRG15 (MORF4L1), which has been implicated in promoting proliferation of neural precursor cells. I have demonstrated that C. elegans with reduced proteasome activity, or that lack RFP-1 expression, have increased levels of MRG-1 and cause a shift towards increased proliferation in sensitized genetic backgrounds. Likewise, reduction of MRG-1 partially suppresses stem cell over-proliferation. Analysis in tissue culture cells further supports that RFP-1 ubiquitinates and directly targets MRG-1 for degradation by the proteasome. I discovered that MRG-1 levels are controlled independently of the spatially regulated GLP-1/Notch signaling pathway, which is the primary signal controlling the extent of stem cell proliferation in the C. elegans germline. Therefore, I have identified MRG-1 as a key player in regulating the proliferation versus differentiation decision and determined that its activity is controlled, at least in part, through proteasomal degradation. Furthermore in a second project, using Stable Isotope Labeling of Amino Acids in C. elegans (SILAC) proteomics, I have identified proteins in the C. elegans germline and quantified the abundance of proteins that are enriched in the stem cell population. I have identified eight putative proliferation-promoting proteins that cause suppression of proliferation when their function is reduced. Stem cells contain the therapeutic power to rejuvenate human life. However, this is possible only if we can clearly understand their behaviour. Through this kind of research we can obtain deeper insight into the functioning of stem cells, necessary to harness their untapped potential.
Weismann-Netter-Stuhl syndrome is a rarely reported cause of bowed legs; hence a thorough clinical and radiological examination is needed for its diagnosis. In view of the paucity of reports guiding ...the treatment of the deformity, we propose a one-level/two-level osteotomy with intramedullary nail fixation as a treatment modality for the tibial bowing.
Telemetry Simulation & Analysis Gupta, Pratyush; Gupta, Harshal; Ushasukhanya, S. ...
2023 International Conference on Networking and Communications (ICNWC),
2023-April-5
Conference Proceeding
The purpose of this project is to analyze telemetry data for a comprehensive analysis of driving conditions, range, electric motor performance, and other characteristics linked to telemetry packets ...based on information transmitted by the car Telematics Control Unit (TCU). The strategy is intended to assist us in eliminating the requirement for sending test vehicles out to verify every tiny modification made by the software team and its impact on the core functionality of the vehicle systems. In the automobile industry, telemetry provides very useful information about a driver's performance and vehicle by collecting telemetry data from sensors within the vehicle TCU. This is done for various reasons varying from, insurance rating and predictive maintenance to staff compliance monitoring. Telemetry analysis has taken over the complete control of the automobile sector. These conclusions are driving the automobile industry toward some unbelievable and never-before results. The automobile industry is on a roll and Telemetry Analysis is its wheels. Telemetry Analysis has helped the automobile industry achieve things that were beyond our imaginations from analyzing the trends to understanding the supply chain management, from taking care of its customers to turning our wildest dream of connected cars into a reality, Vehicle Telemetry Analysis is well and truly driving the automobile industry crazy.
The objective of this work is to discuss the case studies that answer the issues related to Enterprise/warehouse in the medical sector and respond to the anomaly related to health in ...households/medical care organizations. The case studies use industry-perspective Internet of Things hardware, technologies, platforms, and UIs. The work also introduces the three giants of Internet of Things Frameworks, Azure from Microsoft, AWS Internet of Things from Amazon, IBM which provides Internet of Things solutions with the most trusted deployments.
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