Vibrio harveyi
, which belongs to family
Vibrionaceae
of class
Gammaproteobacteria
, includes the species
V. carchariae
and
V. trachuri
as its junior synonyms. The organism is a well-recognized and ...serious bacterial pathogen of marine fish and invertebrates, including penaeid shrimp, in aquaculture. Diseased fish may exhibit a range of lesions, including eye lesions/blindness, gastro-enteritis, muscle necrosis, skin ulcers, and tail rot disease. In shrimp,
V. harveyi
is regarded as the etiological agent of luminous vibriosis in which affected animals glow in the dark. There is a second condition of shrimp known as
Bolitas negricans
where the digestive tract is filled with spheres of sloughed-off tissue. It is recognized that the pathogenicity mechanisms of
V. harveyi
may be different in fish and penaeid shrimp. In shrimp, the pathogenicity mechanisms involved the endotoxin lipopolysaccharide, and extracellular proteases, and interaction with bacteriophages. In fish, the pathogenicity mechanisms involved extracellular hemolysin (encoded by duplicate hemolysin genes), which was identified as a phospholipase B and could inactivate fish cells by apoptosis, via the caspase activation pathway.
V. harveyi
may enter the so-called viable but nonculturable (VBNC) state, and resuscitation of the VBNC cells may be an important reason for vibriosis outbreaks in aquaculture. Disease control measures center on dietary supplements (including probiotics), nonspecific immunostimulants, and vaccines and to a lesser extent antibiotics and other antimicrobial compounds.
Circulating tumor cells (CTCs) have been a non-invasive technique which allows investigation of tumor characteristics. The purpose of this study was to investigate the relationship between ...circulating tumor cells and colorectal cancer.
The clinical data of 617 patients with colorectal cancer from October 2019 to March 2022 were retrospectively collected to analyze the correlation between CTCs and clinicopathologic characteristics.
The CTCs value increased with the progression of Tumor(T) stage,Metastasis(M) stage and Tumor Node Metastasis(TNM) stage (P < 0.05), but not with Node (N) stage (P > 0.05). Binary logistic regression analysis showed that CTCs, CEA, CA125 and CA199 were independent risk factors for CRC metastasis. Compared with CTCs, CEA, CA125 and CA199, the Logistic model had the highest AUC (AUC = 0.778,95%CI: 0.732-0.824), and the specificity and sensitivity were 82.9% and 63.2%, respectively. After operation, chemo-radiotherapy and other treatment for CRC, CTCs and CEA were significantly decreased compared with before treatment (P < 0.05). In addition, Spearman Correlation showed significant correlation between CTCs and IgG (P = 0.000).
CTCs, CEA, CA125 and CA199 were independent risk factors for CRC metastasis.CTCs can be used for the prediction of tumur metastasis, and the evaluation of therapeutic effect.
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•ZnSe QDs were synthesized and used to assemble ECL sensor.•A novel amplification strategy was designed for detection of hydroquinone in water.•The ECL energy transfer between the ...excited ZnSe QDs and benzoquinone was observed.•The proposed method is sensitive and selective.
We proposed a signal amplification strategy designed for sensitive electrochemiluminescence (ECL) detection of hydroquinone (HQE) in water. The detection mechanism is based on the ECL energy transfer (ET) can generate between the excited ZnSe quantum dots (QDs) and the benzoquinone (BQE) obtained by oxidation of hydroquinone. The ECL-ET resulted in the quenching effect of the cathodic ECL of ZnSe QDs using S2O82− as co-reactant. Because the graphene of outstanding electronic conductivity can accelerate the electron transfer rate between the ZnSe QDs on electrode and S2O82− in the electrolyte solution, so that larger than 3-fold enhance in ECL intensity was obtained after ZnSe QDs decorated on graphene. Under the optimal conditions, the linear range for hydroquinone was 1 × 10−9–1 × 10−6 mol/L with a detection limit of 3.4 × 10−10 mol/L (S/N = 3). The proposed ECL sensor showed the performance advantages of fine selectivity, high sensitivity, low detection limit, wide linear range and good reproducibility. It will hold an enormous potential in the field of environmental analysis.
The use of trisubstituted selenonium salts as organic Lewis acids in electrophilic halogenation and aldol‐type reactions has been developed. The substrate scope is broad. The reaction conditions are ...mild and compatible with various functionalities. This study opens a new avenue for the development of nonmetallic Lewis acid catalysis.
Se salt: The use of trisubstituted selenonium salts as organic Lewis acids in electrophilic halogenation and aldol‐type reactions has been developed. The substrate scope is broad. The reaction conditions are mild and compatible with various functionalities. This study opens a new avenue for the development of non‐metallic Lewis acid catalysis.
The perennial herb Aconitum sinomontanum Nakai (Ranunculaceae) has been utilized as a traditional oriental medicine in China for numerous years. The principal pharmacological constituent of A. ...sinomontanum, lappaconitine (LA), exhibits analgesic, anti-inflammatory, anti-tumor, anti-arrhythmic, and anti-epileptic activities. Due to its potent efficacy and non-addictive nature, LA is widely utilized in the management of cancer pain and postoperative analgesia. This review encompasses the research advancements pertaining to LA including extraction methods, separation techniques, pharmacological properties, chemical modifications, and clinical applications. Additionally, it offers insights into the potential applications and current challenges associated with LA to facilitate future research endeavors.
Wireless Power Transfer (WPT) technology can provide real-time power for many terminal devices in Internet of Things (IoT) through millimeter Wave (mmWave) to support applications with large capacity ...and low latency. Although the intelligent reflecting surface (IRS) can be adopted to create effective virtual links to address the mmWave blockage problem, the conventional solutions only adopt IRS in the downlink from the Base Station (BS) to the users to enhance the received signal strength. In practice, the reflection of IRS is also applicable to the uplink to improve the spectral efficiency. It is a challenging to jointly optimize IRS beamforming and system resource allocation for wireless energy acquisition and information transmission. In this paper, we first design a Low-Energy Adaptive Clustering Hierarchy (LEACH) clustering protocol for clustering and data collection. Then, the problem of maximizing the minimum system spectral efficiency is constructed by jointly optimizing the transmit power of sensor devices, the uplink and downlink transmission times, the active beamforming at the BS, and the IRS dynamic beamforming. To solve this non-convex optimization problem, we propose an alternating optimization (AO)-based joint solution algorithm. Simulation results show that the use of IRS dynamic beamforming can significantly improve the spectral efficiency of the system, and ensure the reliability of equipment communication and the sustainability of energy supply under NLOS link.
In the sixth generation (6G) era, intelligent machine network (IMN) applications, such as intelligent transportation, require collaborative machines with communication, sensing, and computation (CSC) ...capabilities. This article proposes an integrated communication, sensing, and computation (ICSAC) framework for 6G to achieve the reciprocity among CSC functions to enhance the reliability and latency of communication, accuracy and timeliness of sensing information acquisition, and privacy and security of computing to realize the IMN applications. Specifically, the sensing and communication functions can merge into unified platforms using the same transmit signals, and the acquired real-time sensing information can be exploited as prior information for intelligent algorithms to enhance the performance of communication networks. This is called the computing-empowered integrated sensing and communications (ISAC) reciprocity. Such reciprocity can further improve the performance of distributed computation with the assistance of networked sensing capability, which is named the sensing-empowered integrated communications and computation (ICAC) reciprocity. The above ISAC and ICAC reciprocities can enhance each other iteratively and finally lead to the ICSAC reciprocity. To achieve these reciprocities, we explore the potential enabling technologies for the ICSAC framework. Finally, we present the evaluation results of crucial enabling technologies to show the feasibility of the ICSAC framework.
Abdominal fat (AF) is one of the most important economic traits in chickens. Excessive AF in chickens will reduce feed utilization efficiency and negatively affect reproductive performance and ...disease resistance. However, the regulatory network of AF deposition needs to be further elucidated. In the present study, 300 one-day-old female Wannan chickens were reared to 17 wk of age, and 200 Wannan hens were selected to determine the abdominal fat percentage (AFP). Twenty AF tissue samples with the lowest AFP were selected as the low abdominal fat group (L-AFG), and 20 AF tissue samples with the highest AFP were selected as the high abdominal fat group (H-AFG). Eleven samples from L-AFG and 14 samples from H-AFG were selected for RNA-seq and used for weighted gene co-expression network analysis (WGCNA). Among the 25 RNA-seq samples, 5 samples with the lowest and highest AFP values were selected for differential expression gene analysis. Compared with the L-AFG, 225 and 101 genes were upregulated and downregulated in the H-AFG, respectively. A total of 20,503 genes were used to construct the WGCNA, and 44 co-expression gene modules were identified. Among these modules, 3 modules including turquoise, darkorange2, and floralwhite were identified as significantly associated with AFP traits. Furthermore, several genes including acyl-CoA oxidase 1 (ACOX1), stearoyl-CoA desaturase (SCD), aldehyde dehydrogenase 6 family member A1 (ALDH6A1), jun proto-oncogene, AP-1 transcription factor subunit (JUN), and fos proto-oncogene, AP-1 transcription factor subunit (FOS) involved in the “PPAR signaling pathway,” “fatty acid metabolism,” and “MAPK signaling pathway” were identified as central regulators that contribute to AF deposition. These results provide valuable information for further understanding of the gene expression and regulation of AF traits and contribute to future molecular breeding for AF in chickens.
Diabetic nephropathy (DN) is a severe complication of diabetes and the leading cause of end-stage renal disease and death. Germacrone (Ger) possesses anti-inflammatory, antioxidant and anti-DN ...properties. However, it is unclear whether the improvement in kidney damage caused by Ger in DN mice is related to abnormal compositions and metabolites of the gut microbiota. This study generates a mouse model of DN to explore the potent therapeutic ability and mechanism of Ger in renal function by 16S rRNA sequencing and untargeted fecal metabolomics. Although there is no significant change in microbiota diversity, the structure of the gut microbiota in the DN group is quite different.
and
are elevated in the model group but significantly decreased after Ger intervention (
<0.05). Under the treatment of Ger, no significant differences in the diversity and richness of the gut microbiota are observed. An imbalance in the intestinal flora leads to the dysregulation of metabolites, and non-targeted metabolomics data indicate high expression of stearic acid in the DN group, and oleic acid could serve as a potential marker of the therapeutic role of Ger in the DN model. Overall, Ger improves kidney injury in diabetic mice, in part potentially by reducing the abundance of
and
, as well as regulating the associated increase in metabolites such as oleic acid, lithocholic acid and the decrease in stearic acid. Our research expands the understanding of the relationship between the gut microbiota and metabolites in Ger-treated DN. This contributes to the usage of natural products as a therapeutic approach for the treatment of DN via microbiota regulation.
Purpose
The aim of the current study was to identify the relationship between body composition changes during neoadjuvant therapy (NAT) and the treatment efficiency of NAT in gastrointestinal cancer ...(GC) patients.
Methods
From January 2015 to July 2020, 277 GC patients treated with NAT had included for retrospective analysis. The body mass index (BMI) and computed tomography (CT) imaging before and after NAT were recorded. The BMI change optimal cut-off value were calculated by ROC curve. Balancing essential characteristic variables using propensity score matching (PSM) method. Exploring the association between BMI changes and tumor response to NAT using logistic regression analysis. The survival outcome of matched patients between different BMI change groups was compared.
Results
A cutoff point of BMI change >2% during NAT was defined as BMI loss. Among the 277 patients, 110 (39.7%) patients showed BMI change with a loss after NAT. In total, 71 pairs of patients were selected for further analysis. The median follow-up time was 22 months (range 3 to 63 months). Univariate and multivariate logistic regression analyses in matched cohort showed that BMI change was a prognostic factor for tumor response after NAT in GC patients (odds ratio (OR), .471; 95% confidence interval (CI), .233-.953; P = .036). In addition, patients who experienced BMI loss after NAT showed worse overall survival than those who had BMI gain or stable.
Conclusion
BMI loss during NAT probably may has negative effects on NAT efficiency and survival for gastrointestinal cancer patients. It is necessary to monitor and maintain weight for patients during treatment.
Graphical Abstract
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