Deep Convolutional Neural Networks have been adopted for salient object detection and achieved the state-of-the-art performance. Most of the previous works however focus on region accuracy but not on ...the boundary quality. In this paper, we propose a predict-refine architecture, BASNet, and a new hybrid loss for Boundary-Aware Salient object detection. Specifically, the architecture is composed of a densely supervised Encoder-Decoder network and a residual refinement module, which are respectively in charge of saliency prediction and saliency map refinement. The hybrid loss guides the network to learn the transformation between the input image and the ground truth in a three-level hierarchy -- pixel-, patch- and map- level -- by fusing Binary Cross Entropy (BCE), Structural SIMilarity (SSIM) and Intersection-over-Union (IoU) losses. Equipped with the hybrid loss, the proposed predict-refine architecture is able to effectively segment the salient object regions and accurately predict the fine structures with clear boundaries. Experimental results on six public datasets show that our method outperforms the state-of-the-art methods both in terms of regional and boundary evaluation measures. Our method runs at over 25 fps on a single GPU. The code is available at: https://github.com/NathanUA/BASNet.
Nature provides much inspiration for developing soft millirobots. However, compared with smart and adaptations of lives in nature, these robotic systems still suffer from insufficiency of ...intelligence. Here, a new untethered soft millirobot with magnetic actuation in the head and function in the tail is presented via implementing control, actuation, and sensing directly in the materials, thereby endowing robots with multimodal locomotion and environment‐adaptive functions. Due to the soft and asymmetric structure, the millirobot not only shows robust multimodal locomotion, including controllable and transformable crawling, swinging and rolling, but also achieves an excellent capability of helical propulsion in water. Moreover, the robot also possesses outstanding obstacle‐crossing abilities, including helically propelling over obstacles (>2 body length), crawling within a 2 mm height tunnel and swinging through a 450 µm width channel. Furthermore, the robot can even squeeze its body to crawl through a tube easily via near‐infrared irradiation, which triggers the osmotic shrinking of its body. Notably, the robots also possess extraordinary environment‐adaptive functions, for example, leptocephali‐like optical camouflage in water, octopus‐like controllable delivery and variable appearance via visible color–shifting for interaction with the changing environment. These smart robotic systems would be of benefit in various fields via seamless integration of bioinspired design and smart materials.
The bioinspired hydrogel‐based millirobots not only possess excellent capabilities of controllable and transformable locomotion, but also outstanding obstacle‐crossing abilities. Furthermore, the robots even have extraordinary environment‐adaptive functions, including leptocephali‐like optical camouflage, octopus‐like controllable delivery, and variable appearance via visible color–shifting for interaction with the changing environment, which would benefit a wide range of areas such as biomedical and environmental fields.
Microrobots have great potential for multiple applications, such as targeted drug delivery and micromanipulation. Several kinds of microrobots assembled by nanoparticles have been proposed by ...researchers. However, they are difficult to adapt for complex environments, for example, traversing porous materials or climbing over obstacles. Many such environments require multimodal motion control. In this article, we proposed a needle-like microrobot assembled by ferromagnetic nanoparticles, which enables three types of locomotion, defined as axial motion, lateral motion, and rolling motion. The influence of velocities by input frequencies and the lengths of the microrobots are investigated theoretically and experimentally. Moreover, visual feedback path-following control methods are designed for each motion type, and experimentally verified. The needle-like microrobots enable traversing the nonwoven layer of a clinical mask and a seaweed silk barrier by axial motion, which showed a separation and a reaggregation during the traversing process. The needle-like microrobots with rolling motion are capable of climbing over obstacles, which expands its motion scene from 2-D to 3-D. They can overcome even higher obstacles by assembling themselves to form the longer needle-like microrobots. We further expect that with the proposed multimodal motion control of nanoparticle microrobots, they may achieve complex tasks at the microscale.
Untethered microrobots have attracted extensive attention due to their potential for biomedical applications and micromanipulation at the small scale. Soft microrobots are of great research ...importance because of their highly deformable ability to achieve not only multiple locomotion mechanisms but also minimal invasion to the environment. However, the existing microrobots are still limited in their ability to locomote and cross obstacles in unstructured environments compared to conventional legged robots. Nature provides much inspiration for developing miniature robots. Here, we propose a bionic quadruped soft thin-film microrobot with a nonmagnetic soft body and 4 magnetic flexible legs. The quadruped soft microrobot can achieve multiple controllable locomotion modes in the external magnetic field. The experiment demonstrated the robot's excellent obstacle-crossing ability by walking on the surface with steps and moving in the bottom of a stomach model with gullies. In particular, by controlling the conical angle of the external conical magnetic field, microbeads gripping, transportation, and release of the microrobot were demonstrated. In the future, the quadruped microrobot with excellent obstacle-crossing and gripping capabilities will be relevant for biomedical applications and micromanipulation.
•Dual-frequency ultrasound combined with PMS had a synergistic effect.•Three new degradation pathways for tetracycline degradation were proposed.•O2∙-, •OH and SO4∙- were the main contributors to the ...oxidation process.•Dual-frequency ultrasound provided a higher number of active bubbles.
Tetracycline has received a great deal of interest for the harmful effects of substance abuse on ecosystems and humanity. The effects of different processes on the degradation of tetracycline were compared, with dual-frequency ultrasound (DFUS) in combination with peroxymonosulfate (PMS) being the most effective for the tetracycline degradation. Free radical scavenging experiments showed that O2∙-,SO4∙- and •OH were the main reactive radicals in the degradation of tetracycline. According to the major intermediates of tetracycline degradation identified, three possible degradation pathways were proposed, which are of significance for translational studies of tetracycline degradation. Notably, these intermediates were found to be significantly less toxicity. The number of active bubbles in the degradation vessel was calculated using a semi-empirical formula, and a higher value of 1.44 × 108 L-1s−1 of bubbles was obtained when using dual-frequency ultrasound at 20 kHz (210 W/L) and 80 kHz (85.4 W/L). Therefore, compared to 20 kHz, although the yield of strong oxidizing substances from individual active bubbles decreased slightly, a significant increment of the number of active bubbles still resulted in a higher synergistic effect, and the combination of DFUS and PMS should be effective in promoting the generation of reactive free radicals and mass transfer processes within the degradation vessel, which provides a method for efficient removal of tetracycline from wastewater.
Sepsis‐induced liver injury is recognized as a key problem in intensive care units. The gut microbiota has been touted as an important mediator of liver disease development; however, the precise ...roles of gut microbiota in regulating sepsis‐induced liver injury are unknown. Here, we aimed to investigate the role of the gut microbiota in sepsis‐induced liver injury and the underlying mechanism. Cecal ligation and puncture (CLP) was used to induce polymicrobial sepsis and related liver injury. Fecal microbiota transplantation (FMT) was used to validate the roles of gut microbiota in these pathologies. Metabolomics analysis was performed to characterize the metabolic profile differences between sepsis‐resistant (Res; survived to 7 days after CLP) and sepsis‐sensitive (Sen; moribund before or approximately 24 hours after CLP) mice. Mice gavaged with feces from Sen mice displayed more‐severe liver damage than did mice gavaged with feces from Res mice. The gut microbial metabolic profile between Sen and Res mice was different. In particular, the microbiota from Res mice generated more granisetron, a 5‐hydroxytryptamine 3 (5‐HT3) receptor antagonist, than the microbiota from Sen mice. Granisetron protected mice against CLP‐induced death and liver injury. Moreover, proinflammatory cytokine expression by macrophages after lipopolysaccharide (LPS) challenge was markedly reduced in the presence of granisetron. Both treatment with granisetron and genetic knockdown of the 5‐HT3A receptor in cells suppressed nuclear factor kappa B (NF‐кB) transactivation and phosphorylated p38 (p‐p38) accumulation in macrophages. Gut microbial granisetron levels showed a significantly negative correlation with plasma alanine aminotransferase (ALT)/aspartate aminotransferase (AST) levels in septic patients. Conclusion: Our study indicated that gut microbiota plays a key role in the sensitization of sepsis‐induced liver injury and associates granisetron as a hepatoprotective compound during sepsis development.
Trichoderma asperellum is one of the species which can be isolated from contaminated Pleurotus ostreatus cultivation substrate with green mold disease. This study focused on the relationship between ...high temperature and infectivity of T. asperellum to P. ostreatus. Antagonism experiments between T. asperellum and P. ostreatus mycelia revealed that high temperature-treated P. ostreatus mycelia were more easily infected by T. asperellum and covered by conidia. Microscopic observation also showed that P. ostreatus mycelia treated with high temperature could adsorb more T. asperellum conidia. Furthermore, conidia obtained from T. asperellum mycelia grown at 36°C featured higher germination rate compared with that incubated at 28°C. High temperature-treated T. asperellum mycelia can produce conidia in shorter periods, and T. asperellum mycelia were less sensitive to high temperature than P. ostreatus. Deactivated P. ostreatus mycelia can induce T. asperellum cell wall-degrading enzymes (CWDEs) and P. ostreatus mycelia subjected to high temperature showed induced CWDEs more effective than those incubated at 28°C. Moreover, T. asperellum showed higher CWDEs activity at high temperature. In dual cultures, hydrogen peroxide (H2O2) increased after 36°C, and high concentration of H2O2 could significantly inhibit the growth of P. ostreatus mycelia. In summary, our findings indicated for the first time that high temperature can induce a series of mechanisms to enhance infection abilities of T. asperellum to P. ostreatus mycelia and to cause Pleurotus green mold disease.
An integrated modeling framework coupling the discrete element method for powder spreading, finite volume method for powder bed melting, and an extended cellular automaton method for grain structure ...evolution during solidification is proposed. In this framework, the initial grain structure of both the substrate and metal powders can be taken into account and used to capture epitaxial and competitive grain growth. The framework is used to provide an in-depth understanding of microstructure development in Ti-6Al-4V during the selective electron beam melting process. The complex process of grain growth during deposition of multiple tracks and multiple layers is modeled through an analysis restarting scheme. The epitaxial growth of grains from pre-existing grains, in particular the grains of partially melted powders, is reproduced. The mechanism of microstructure development within the overlap region of consecutive tracks and layers for various scan strategies is revealed. The simulation results are in qualitative agreement with experimental observation in the literature. The proposed modeling framework is a powerful tool to guide optimal process parameters that lead to designed, site-specific microstructure control and therefore to tailored mechanical properties of parts fabricated by the powder bed fusion additive manufacturing process.
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•A powder-scale integrated modeling framework is developed for microstructure prediction.•The effect of initial microstructure on solidification microstructure is identified•Varying the scan strategy effects the texture.•A “skin-layer” is shown to epitaxially grow from partially melted powder.
Phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) is the first key enzyme in the phenylpropanoid pathway. The pal gene has been widely studied in plants and participates in plant growth, development and ...defense systems. However, in Pleurotus ostreatus, the biological functions of pal during organismal development and exposure to abiotic stress have not been reported.
In this study, we cloned and characterized the pal1 (2232 bp) and pal2 (2244 bp) genes from the basidiomycete P. ostreatus CCMSSC 00389. The pal1 and pal2 genes are interrupted by 6 and 10 introns, respectively, and encode proteins of 743 and 747 amino acids, respectively. Furthermore, prokaryotic expression experiments showed that PAL enzymes catalyzed the conversion of L-phenylalanine to trans-cinnamic acid. The function of pal1 and pal2 was determined by constructing overexpression (OE) and RNA interference (RNAi) strains. The results showed that the two pal genes had similar expression patterns during different developmental stages. The expression of pal genes was higher in the reproductive growth stage than in the vegetative growth stage. And the interference of pal1 and pal2 delayed the formation of primordia. The results of heat stress assays showed that the RNAi-pal1 strains had enhanced mycelial tolerance to high temperature, while the RNAi-pal2 strains had enhanced mycelial resistance to H
O
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These results indicate that two pal genes may play a similar role in the development of P. ostreatus fruiting bodies, but may alleviate stress through different regulatory pathways under heat stress.
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