The emergence of recombination-activating genes (RAGs) in jawed vertebrates endowed adaptive immune cells with the ability to assemble a diverse set of antigen receptor genes. In contrast, innate ...lymphocytes, such as natural killer (NK) cells, are not believed to require RAGs. Here, we report that NK cells unable to express RAGs or RAG endonuclease activity during ontogeny exhibit a cell-intrinsic hyperresponsiveness but a diminished capacity to survive following virus-driven proliferation, a reduced expression of DNA damage response mediators, and defects in the repair of DNA breaks. Evidence for this novel function of RAG has also been observed in T cells and innate lymphoid cells (ILCs), revealing an unexpected role for RAG proteins beyond V(D)J recombination. We propose that DNA cleavage events mediated by RAG endow developing adaptive and innate lymphocytes with a cellular “fitness” that safeguards their persistence later in life during episodes of rapid proliferation or cellular stress.
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•RAG expression in NK cell ontogeny defines a cell-intrinsic differentiation program•RAG plays a novel role in the functional specialization of NK cell subsets•RAG-deficient lymphocytes exhibit a reduced DNA damage response and cellular fitness•A physiological role for RAG proteins exists outside of V(D)J recombination
A novel role for the RAG proteins, unrelated to antigen receptor generation but, rather, regulating the cellular fitness of NK cells, adds to a growing body of evidence implicating “controlled” DNA damage in the regulation of gene expression, cell development, and cell fate in eukaryotes.
Abstract
The
Moringa oleifera
leaves are a promising candidate plant protein source for fish feeds because of its high crude protein content, varying from 21% to 32%. However, because of high content ...of crude fiber in moringa, it can reduce the digestibiliy of the nutrients. Therefore, there is a need for intervention to improve the quality of
M. oleifera
leaves. The current research was carried out to assess the nutrient quality of
M. oleifera
leaf meal (MOLM) following fermentation with
Aspergillus niger
at different dosages as alternative feed ingredients for fish. In this study, a completely randomized design with four treatments and three repetitions was used. The treatments were as folllows: F0 = Fermentation of MOLM without
A. niger
(control), F1 = Fermentation of MOLM with
A. niger
at 2% (w/w) of MOLM, F2 = Fermentation of MOLM with
A. niger
at 4% (w/w) of MOLM and F3 = Fermentation of MOLM with
A. niger
at 6% (w/w) of MOLM. Results showed that MOLM fermented with
A. niger
had a significant effect on crude fiber and crude protein. It can be concluded that MOLM supplemented with 6%
A. niger
could improve crude protein and crude fiber quality of
Moringa oleifera
leaves.
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•The Sidakan diabases are mostly low-K basalts with sabalkaline affinity.•The diabases are tholeiitic oceanic island arc basalts and N-MORB related.•The generation age of Sidakan ...diabases ranges between 34.6 ± 0.84 and 39.2 ± 1.81 Ma.•The Oceanic Island Arc (OIA) was formed during the initial stages of obduction.•The Iraqi Zagros Thrust Zone was formed subsequent to the formation of OIA.
This study presents petrography, geochemistry, mineral chemistry and geochronology of diabase dikes from Sidakan area, northeastern Iraq. The area consists of basalts and diabase dikes alternating with sedimentary rocks, hosted by Walash Metavolcanic Group (WMG) as part of the Zagros Fold-Thrust Zone (ZFTZ). These rocks are dark grey to pale brown in color, fine to medium in grain size, and have ophitic and porphyritic textures. They consist of plagioclase and clinopyroxene as essential minerals and minor olivine as well as their alteration and low-grade metamorphism products including amphibole, albite, epidote, chlorite, serpentine, calcite and sericite, with ilmenite as an accessory mineral. The diabase dikes consist (as average wt.%) of 51.41 SiO2, 15.82 Al2O3, 9.95 FeOt, 6.58 MgO, 9.63 CaO, 3.42 Na2O, 0.87 TiO2, 0.17 MnO, 0.35 K2O, 0.09 P2O5, and 1.92 LOI. The Harker diagrams indicate that the diabases were formed from fractional crystallization of tholeiitic parent magma. The classification diagram Nb/Y vs. Zr/TiO2 indicated that the studied diabases have affinities of subalkaline basalts, while the SiO2 vs. K2O diagram showed that they are divided between the low-K basalt and basaltic andesite. The tectonic discriminant diagrams show that the diabases are oceanic island-arc tholeiites with N-MORB geochemical affinities. The K − Ar age of the diabases is Late Eocene (Bartonian-Priabonian) ranging between 34.6 ± 0.84 to 39.2 ± 1.81 Ma representing their intrusion/crystallization age and possibly the age of the formation of Oceanic Island Arc and/or the initial collision stage between the Arabian-Iranian microplates.
The mammalian immune system has been traditionally subdivided into two compartments known as the innate and the adaptive. T cells and B cells, which rearrange their antigen‐receptor genes using the ...RAG recombinase, comprise the adaptive arm of immunity. Meanwhile, every other white blood cell has been grouped together under the broad umbrella of innate immunity, including NK cells. NK cells are considered innate lymphocytes because of their rapid responses to stressed cells and their ability to develop without receptor gene rearrangement (i.e. in RAG‐deficient mice). However, new findings implicate a critical function for RAG proteins during NK‐cell ontogeny, and suggest a novel mechanism by which controlled DNA breaks during NK‐cell development dictate the fitness, function, and longevity of these cells. This review highlights recent work describing how DNA break events can impact cellular differentiation and fitness in a variety of cell types and settings.
NK Cell Responses Redefine Immunological Memory Adams, Nicholas M; O'Sullivan, Timothy E; Geary, Clair D ...
The Journal of immunology (1950),
10/2016, Volume:
197, Issue:
8
Journal Article
Peer reviewed
Open access
Immunological memory has traditionally been regarded as a unique trait of the adaptive immune system. Nevertheless, there is evidence of immunological memory in lower organisms and invertebrates, ...which lack an adaptive immune system. Despite their innate ability to rapidly produce effector cytokines and kill virally infected or transformed cells, NK cells also exhibit adaptive characteristics such as clonal expansion, longevity, self-renewal, and robust recall responses to antigenic or nonantigenic stimuli. In this review, we highlight the intracellular and extracellular requirements for memory NK cell generation and describe the emerging evidence for memory precursor NK cells and their derivation.
The Northern Zagros Suture Zone (NZSZ), formed as a result of the collision between Arabian and Sanandaj-Sirjan microplate, is considered as part of the Zagros orogenic belt. NZSZ is marked by two ...allochthonous thrust sheets in upward stacking order: lower and upper allochthon. The Bulfat complex is a part of the upper allochthon or “Ophiolite-bearing terrane” of Albian-Cenomenion age (97–105 Ma). Voluminous highly sheared serpentinites associated with ophiolites occur within this upper allochthon. In addition, the Gemo-Qandil Group is characterized by gabbroic to dioritic Bulfat intrusion with a crystallization age spanning from ~45 to ~ 40 Ma, as well as extensive metapelites with contact to the Walash-Naupurdam metavolcanic rocks. Due to the deformation in the Sanandaj-Sirjan Zone along the eastern side of the Iraqi segment of NZSZ, the Gemo-Qandil Group was regionally metamorphosed during late Cretaceous (~ 80 Ma). This tectono-compressional dynamics ultimately caused an oscillatory deformation against Arabian continental margin deposits as well. During these events, gabbro-diorite intrusion with high-grade contact metamorphic aureoles occurred near Bulfat. Thus, there is an overlap between regional and contact metamorphic conditions in the area. The earlier metamorphic characteristic can be seen only in places where the latter contact influence was insignificant. Generally, this can only observed at a distance of more than 2.5 km from the contact. According to petrographic details and field observations, the thermally metamorphosed metapelitic units of the metasediment have been completely assimilated, with only some streaks of biotite and relicts of initial foliation. They strongly resemble amphibolite-grade slices from the regional metamorphic rocks in the region. Metapelitic samples far from the intrusion give similar biotite cooling ages as the intrusive rocks. Thus, they may be affected by the same thermal event.
40
Ar/
39
Ar dating of biotite in metapelite rocks of Bulfat by step-wise heating with laser gave average weighted isotopic ages of 34.78 ± 0.06 Ma. This is interpreted as crystallization/recrystallization age of biotite possibly representing the time of cooling and uplift history of the Bulfat intrusion. Cooling and exhumation rates for the Bulfat gabbro-diorite rocks were estimated as ~ 400 °C/Ma and ~ 3.3 mm/year respectively. According to petrographic details, field observations and Ar/Ar dating concerning the contact metamorphism near Bulfat due to the gabbro-diorite intrusion, no significant deformation is visible during exhumation processes after the Paleogene tectono-thermal event, indicating that isotopic ages of 34.78 ± 0.06 Ma could mark the timing of termination of the island arc activity in the Ophiolite-bearing terrane (upper allochthon).
Penjween ophiolite is one of the ophiolitic complexes of northeastern Iraq. The serpentinites within the Penjween Ophiolite hosts many pods of hornblendite and amphibolite, and dikes of diorite among ...many other igneous bodies. These pods have very sharp contacts with the surrounding mantle serpentinized harzburgites. The hornblendites and amphibolites are usually intimately intergrown together as extremely hard, dark green to black colored, fine-to medium-grained pods with ∼2 x ∼2 x ∼(0.5–1) m dimensions. This study presents petrography, mineral chemistry, whole-rock major and trace element geochemistry, and electron probe microanalyses (EPMA) for the major minerals in the studied rocks. The hornblendite is composed entirely of amphiboles (>99% vol.), meanwhile the amphibolite consists of comparable amounts of amphibole and plagioclase which occasionally occurs as layered rocks with banded texture. The diorite dikes are white in color and consist dominantly of coarse-grained plagioclase and less amphiboles. The amphiboles of these rocks belong to pargasite (Mg# 0.69–0.77) ─ edenite (Mg# 0.74–0.79) endmembers where pargasite is by far the predominant mineral; meanwhile the plagioclase is albite (Ab93.4An6.4Or0.2). The amphiboles are replacement products of pyroxenes indicated from the relict pyroxene within their crystals. The amphiboles are abnormally rich in various dust-like inclusions of transparent minerals like REE-rich epidote, rutile, zircon, apatite, titanite, and ore minerals like ilmenite, and pyrrhotite, oriented along the crystallographic axes and form distinct zones in the core of amphibole crystals. The geochemical characteristics of the studied hornblendite (MgO = 13.07%, Ni = 260 ppm, Mg# = 66.57) as well as the high Sc (33 ppm) and V (254 ppm) concentrations are collectively consistent with a mantle-derived, igneous origin. The primitive-mantle normalized trace elements spidergram showed enrichment (hump) in Ba, Th, U, La, Ce, Pb, and Sr, and depletion (trough) in Nb, Ta, K, and Ti. The chondrite-normalized REE diagram showed enrichment of LREE relative to HREE, indicated from the smooth and steady decrease in the negative slope from LREE towards HREE with a negligible Eu-anomaly. Various tectonic discriminating diagrams showed that the studied hornblendite, amphibolite pods and diorite dikes are of igneous fore-arc origin, formed from calc-alkaline and/or tholeiitic magma within an active continental margin setting. The 40Ar/39Ar laser age of hornblendite is late Paleocene (Thanetian) (57.8 ± 5.1 Ma) which might represent an event during the obduction between the oceanic fore-arc Island and the Arabian Plate during the Late Cretaceous/Paleocene period.
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•The studied rocks are hornblendite, amphibolite and diorite rocks.•The hornblendite is monomineralic and consists of amphiboles.•The amphibolite and diorite consist of comparable amounts of amphibole and plagioclase.•These rocks are calc-alkaline island arc basalts,•The 40Ar/39Ar laser age of the hornblendite is late Paleocene (Thanetian) (57.8 ± 5.1 Ma).
The amphibolite and hornblendite dikes occur as part of the igneous complexes of Bulfat Ophiolite within the Zagros Suture Zone, NE Iraq. The igneous complex is part of the upper allochthonous ...ophiolite terrane within the second unit of the Penjween-Walash Subzone. This article discusses the petrogenetic characteristics and tectonic setting of the amphibolite rocks based on their petrography, mineral chemistry, and whole-rock geochemistry. Petrographically, both banded and massive amphibolites reveal that the main mineral constituents are amphibole and plagioclase with accessory clinopyroxene, opaques, sphene, apatite, rutile, zircon, sericite, epidote and chlorite; meanwhile the hornblendites consist of >90% amphibole with minor amounts of pyroxene, opaques, plagioclase, titanite, quartz, apatite, zircon, and chlorite. The amphibolites are divided into two types, banded (foliated) and massive, (non-foliated). The dominat textures of amphibolites are porphyroblastic, granoblastic, poikiloblastic, nematoblastic, and blasto-ophitic; meanwhile those of hornblendites are equigranular, poikilitic, cataclastic, and intergranular. The electron microprobe analysis (EPMA) showed that the amphiboles of the banded amphibolite range between tschermakite, magnesio-hornblende and pargasite; and those of the massive amphibolites range between magnesio-hornblende and actinolite; meanwhile those of hornblendites are dominantly tschermakite with minor magnesio-hornblende and pargasite. The plagioclase of the banded amphibolites is mainly oligoclase and andesine; and those of the massive amphibolites are mostly anorthite. Chemical classification diagrams indicated that the studied rocks are had low alkalies (Na2O+K2O), tholeiitic, ultrabasic to basic, alkaline to subalkaline and divided between foidite, picrobasalt and basalt. Various tectonic discriminating diagrams showed that most of studied rocks are mid-ocean ridge basalt (MORB). The studied amphibolites have been subjected to amphibolite facies metamorphism.
Cancer is the second cause of death after cardiovascular diseases. With due attention to rapid progress in the phytochemical study of plants, they are becoming popular because of their anticancer ...effects. The aim of this study was to investigate the effective medicinal plants in the treatment of cancer and study their mechanism of action. In order to gather information the keywords “traditional medicine,” “plant compounds,” “medicinal plant,” “medicinal herb,” “toxicity,” “anticancer effect,” “cell line,” and “treatment” were searched in international databases such as ScienceDirect, PubMed, and Scopus and national databases such as Magiran, Sid, and Iranmedex, and a total of 228 articles were collected. In this phase, 49 nonrelevant articles were excluded. Enhancement P53 protein expression, reducing the expression of proteins P27, P21, NFκB expression and induction of apoptosis, inhibition of the PI3K/Akt pathway, and reduction of the level of acid phosphatase and lipid peroxidation are the most effective mechanisms of herbal plants that can inhibit cell cycle and proliferation. Common treatments such as radiotherapy and chemotherapy can cause some complications. According to results of this study, herbal extracts have antioxidant compounds that can induce apoptosis and inhibit cell proliferation by the investigated mechanisms.
Abstract
Student scholarship through Indonesian smart cards (ISC) is a cash donation scholarship for all student in range 6-21 years old as a solution of destitute child or potential dropouts. ...Currently the large student data stored in educational database. The data was used to determine feasible receiver ISC. Manual prediction by human take long time and potentially human error. This paper aim to predict feasible receiver ISC by using educational data mining. Source of data come from a senior high school database in Riau Province, Indonesia. In this paper we compared two algorithms (Naïve Bayes and ID3) to predict receiver ISC. Eighty percent process in this paper is data pre-processing. Information of other scholarship is the most influences variable to predict student scholarship. ID3 algorithm classification has accuracy 83 percent and f1 score 71 percent. While Naive Bayes Classification has accuracy 89 percent and f1 score 72 percent. In this case Naïve Bayes algorithm is better in ID3 algorithm.
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