Zingiberaceae plants are well known for their use in ethnomedicine. Curcuma mutabilis Škorničk., M. Sabu & Prasanthk., is an endemic Zingiberaceae species from Western Ghats of Kerala, India. Here, ...we report for the first time, the anticancer potential of petroleum ether extract from C. mutabilis rhizome (CMRP) and a novel labdane diterpenoid, (E)-14, 15-epoxylabda-8(17), 12-dien-16-al (Cm epoxide) isolated from it. CMRP was found to be a mixture of potent bioactive compounds including Cm epoxide. Both the extract and the compound displayed superior antiproliferative activity against several human cancer cell lines, without any display of cytotoxicity towards normal human cells such as peripheral blood derived lymphocytes and erythrocytes. CMRP treatment resulted in phosphatidylserine externalization, increase in the levels of intracellular ROS, Ca
, loss of mitochondrial membrane potential as well as fragmentation of genomic DNA. Analyses of transcript profiling and immunostained western blots of extract-treated cancer cells confirmed induction of apoptosis by both intrinsic and extrinsic pathways. The purified compound, Cm epoxide, was also found to induce apoptosis in many human cancer cell types tested. Both CMRP and the Cm epoxide were found to be pharmacologically safe in terms of acute toxicity assessment using Swiss albino mice model. Further, molecular docking interactions of Cm epoxide with selected proteins involved in cell survival and death were also indicative of its druggability. Overall, our findings reveal that the endemic C. mutabilis rhizome extract and the compound Cm epoxide isolated from it are potential candidates for development of future cancer chemotherapeutics.
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•Valorization of agro-industrial wastes has been comprehensively discussed.•Agro-industrial wastes are potential source for value creation.•Methodologies for obtaining products from ...agro-industrial wastes have been appraised.•Applications of LCA in agro-industrial circular bioeconomy have been uncovered.
Energy recovery from waste resources is a promising approach towards environmental consequences. In the prospect of environmental sustainability, utilization of agro-industrial waste residues as feedstock for biorefinery processes have gained widespread attention. In the agro-industry, various biomasses are exposed to different unit processes for offering value to various agro-industrial waste materials. Agro-industrial wastes can generate a substantial amount of valuable products such as fuels, chemicals, energy, electricity, and by-products. This paper reviews the methodologies for valorization of agro-industrial wastes and their exploitation for generation of renewable energy products. In addition, management of agro-industrial wastes and products from agro-industrial wastes have been elaborated. The waste biorefinery process using agro-industrial wastes does not only offer energy, it also offers environmentally sustainable modes, which address effective management of waste streams. This review aims to highlight the cascading use of biomass from agro-industrial wastes into the systemic approach for economic development.
The coming decades may see the large scale deployment of networked cyber-physical systems to address global needs in areas such as energy, water, health care, and transportation. However, as recent ...events have shown, such systems are vulnerable to cyber attacks. Being safety critical, their disruption or misbehavior can cause economic losses or injuries and loss of life. It is therefore important to secure such networked cyber-physical systems against attacks. In the absence of credible security guarantees, there will be resistance to the proliferation of cyber-physical systems, which are much needed to meet global needs in critical infrastructures and services. This paper addresses the problem of secure control of networked cyber-physical systems. This problem is different from the problem of securing the communication network, since cyber-physical systems at their very essence need sensors and actuators that interface with the physical plant, and malicious agents may tamper with sensors or actuators, as recent attacks have shown. We consider physical plants that are being controlled by multiple actuators and sensors communicating over a network, where some sensors could be "malicious," meaning that they may not report the measurements that they observe. We address a general technique by which the actuators can detect the actions of malicious sensors in the system and disable closed-loop control based on their information. This technique, called "watermarking," employs the technique of actuators injecting private excitation into the system, which will reveal malicious tampering with signals. We show how such an active defense can be used to secure networked systems of sensors and actuators.
An important area of cyber-physical systems research is the development of smart ground transportation systems due to their potentially significant impact on safety, the economy, and the environment. ...We propose an approach based on model predictive control (MPC) for the development of provably collision free autonomous ground transportation systems, and present an autonomous intersection management framework. The MPC approach enables a vehicle to generate its own motion locally in time based on an optimization framework, incorporating constraints based on the states of other vehicles in the neighborhood, the speed limit of a road, the maximum values of acceleration and deceleration, etc. Safety and liveness of the traffic are however system-wide properties, not merely neighborhood properties, and the challenge is to augment this distributed optimization with coordination rules that guarantee overall system-wide safety as well as liveness of the traffic. We design two vehicle-to-vehicle (V2V) coordination rules, along with a vehicle-to-infrastructure rule, and establish the system-wide safety and liveness of the autonomous traffic based on each vehicle's MPC motion planner, operating in conjunction with an algorithm that orders vehicles according to their runtime properties. We also conduct a comparative simulation study of the throughput performance at an intersection of the above approach against another popular algorithm, the All-Way STOP.
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•Production of biochar produced through pyrolysis of lignocellulosic biomass has been narrated.•The factors influencing pyrolytic conditions and pyrolytic conversion have been ...discussed.•Reviewed the modification of biochar and mechanism of pollutant adsorption using biochar.•Potential positive aspects of biochar applications towards pollutant removal are also discussed.
Thermochemical processing of biomass results in a producing char, a typical by-product. The char can be termed as biochar when specifically applied as a soil fertility enhancement. Biochar, when utilized efficiently, is basic for enhancing financial viability and also to maintain ecosystem. The properties of carbonized biomass rely upon raw materials (feedstock) and procedure conditions. Biochar shows an incredible potential to effectively handle water contaminants taking into consideration the wide accessibility of feedstock, suitable physical/chemical surface properties and low-cost. Pyrolysis technology for converting lignocellulosic biomass into biochar has emerged as a frontier research domain for the removal of pollutants. This review focused on production of biochar from various sources of lignocellulosic biomass (cellulose, hemicellulose and lignin) and its application in various fields such as agriculture, wastewater treatment process. Biochar is a significant resource however, its application require further examination of its properties and structure and techniques to alter those factors.
Release of pollutants due to inflating anthropogenic activities has a conspicuous effect on the environment. As water is uniquely vulnerable to pollution, water pollution control has received a ...considerable attention among the most critical environmental challenges. Diverse sources such as heavy metals, dyes, pathogenic and organic compounds lead to deterioration in water quality. Demand for the pollutant free water has created a greater concern in water treatment technologies. The pollutants can be mitigated through physical, chemical and biological methodologies thereby alleviating the health and environmental effects caused. Diverse technologies for wastewater treatment with an accentuation on pre-treatment of feedstock and post treatment are concisely summed up. Pollutants present in the water can be removed by processes some of which include filtration, reverse osmosis, degasification, sedimentation, flocculation, precipitation and adsorption. Membrane separation and adsorption methodologies utilized to control water pollution and are found to be more effective than conventional methods and established recovery processes. This audit relatively features different methodologies that show remarkable power of eliminating pollutants from wastewater. This review describes recent research development on wastewater treatment and its respective benefits/applications in field scale were discussed. Finally, the difficulties in the enhancement of treatment methodologies for pragmatic commercial application are recognized and the future viewpoints are introduced.
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•Effects of water pollution are highlighted.•Major toxic pollutants and their sources and effects have been discussed.•The various pollution control technologies have been explained.•Membrane separation and adsorption processes have been highlighted.•The research gap in the treatment methods are highlighted.
This study evaluates the effects of phyto-derived zinc oxide nanoparticles (ZnONPs) on human cancer cells, colon carcinoma HCT 116, and chronic myelogenous leukemic K562, along with normal ...lymphocytes/erythrocytes. The commercial, chemically synthesized ZnONPs (cZnONPs) were also assessed in parallel. Using an eco-friendly approach devoid of harmful chemicals, biogenic nanoparticles were synthesized from aqueous leaf extract of
Spondias pinnata
(SpLZnONPs) by a sol-gel method. Optical, structural, and elemental characterization of both particle types were carried out deploying UV–Vis/photoluminescence spectroscopy, FTIR, XRD, FESEM, HRTEM, and EDX. Both SpLZnONPs and cZnONPs displayed hexagonal wurtzite structure with particle sizes averaging 30 and 48.5 nm, respectively. SpLZnONPs were found to be cytotoxic to both cancer cell types while cZnONPs exhibited toxicity only against HCT 116 cells. Interestingly, the cytomorphological changes and analysis of DNA laddering pattern observed in these treated cells were indicative of simultaneous induction of dual modes of death involving apoptosis and necrosis. Flow cytometric analysis of cell-cycle distribution, clonogenic, wound healing, and comet assays provided evidences of the antiproliferative potential of the tested nanoparticles. Apoptosis induction via oxidative stress-mediated Ca
2+
release, ROS generation, loss of mitochondrial membrane potential, and externalization of phosphatidylserine was also determined biochemically. Relative expression of apoptotic genes was quantified using RT-qPCR and Western blot analysis. Mitotic index analysis, MTT, and hemolytic assays on lymphocytes and erythrocytes clearly revealed the absence of any deleterious effect(s) of SpLZnONPs in these cells compared with the toxicity of the chemically derived cZnONPs, thereby attesting to the biocompatibility and selective action of the biogenic nanoparticles.
Nowadays, the accumulation of toxic heavy metals in soil and water streams is considered a serious environmental problem that causes various harmful effects on plants and animals. Phytoremediation is ...an effective, green, and economical bioremediation approach by which the harmful heavy metals in the contaminated ecosystem can be detoxified and accumulated in the plant. Hyperaccumulators exude molecules called transporters that carry and translocate the heavy metals present in the soil to different plant parts. The hyperaccumulator plant genes can confine higher concentrations of toxic heavy metals in their tissues. The efficiency of phytoremediation relies on various parameters such as soil properties (pH and soil type), organic matters in soil, heavy metal type, nature of rhizosphere, characteristics of rhizosphere microflora, etc. The present review comprehensively discusses the toxicity effect of heavy metals on the environment and different phytoremediation mechanisms for the transport and accumulation of heavy metals from polluted soil. This review gave comprehensive insights into plants tolerance for the higher heavy metal concentration their responses for heavy metal accumulation and the different mechanisms involved for heavy metal tolerance. The current status and the characteristic features that need to be improved in the phytoremediation process are also reviewed in detail.
Three-dimensional bioprinting has emerged as a promising technique in tissue engineering applications through the precise deposition of cells and biomaterials in a layer-by-layer fashion. However, ...the limited availability of hydrogel bioinks is frequently cited as a major issue for the advancement of cell-based extrusion bioprinting technologies. It is well known that highly viscous materials maintain their structure better, but also have decreased cell viability due to the higher forces which are required for extrusion. However, little is known about the effect of the two distinct components of dynamic modulus of viscoelastic materials, storage modulus (G') and loss modulus (G″), on the printability of hydrogel-based bioinks. Additionally, 'printability' has been poorly defined in the literature, mostly consisting of gross qualitative measures which do not allow for direct comparison of bioinks. This study developed a framework for evaluating printability and investigated the effect of dynamic modulus, including storage modulus (G'), loss modulus (G″), and loss tangent (G″/G') on the printing outcome. Gelatin and alginate as model hydrogels were mixed at various concentrations to obtain hydrogel formulations with a wide range of storage and loss moduli. These formulations were then evaluated for the quantitatively defined values of extrudability, extrusion uniformity, and structural integrity. For extrudability, increasing either the loss or storage modulus increased the pressure required to extrude the bioink. A mathematical model relating the G' and G″ to the required extrusion pressure was derived based on the data. A lower loss tangent was correlated with increased structural integrity while a higher loss tangent correlated with increased extrusion uniformity. Gelatin-alginate composite hydrogels with a loss tangent in the range of 0.25-0.45 exhibited an excellent compromise between structural integrity and extrusion uniformity. In addition to the characterization of a common bioink, the methodology introduced in this paper could also be used to evaluate the printability of other bioinks in the future.
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