The thermal conditions of soil are important in practical agricultural production. The characteristics of heat flux, moisture content, thermal conductivity, and other soil parameters vary with ...temperature. This study uses VADOSE/W to create a model of heat transmission between soil and atmosphere, simulating daily changes in soil temperature using sand mulching. By using the published data to verify the model, the results show that the fitting effect is good and the reliability of the model is verified. We also used this model to determine the temporal and spatial distributions of soil temperature, temperature differences, and a temperature gradient for sand mulching and bare soil during the growing season of spring wheat. These results indicated that the sand mulching preserved heat at night. The difference in temperature at each depth and the temperature gradient became negative with the gradual increase in solar altitude. The difference in soil temperature at each depth and the temperature gradient tended to become positive by 22:00. These results indicated that the sand mulch effectively maintained the soil temperature in the morning and night during the growing season and impeded the transfer of heat at mid-day. This study provides a new method for determining the transfer of heat in sand-mulched soil, which can guide the effective regulation of soil temperature.
Rift Valley fever virus (RVFV), a Phlebovirus with a genome consisting of three single-stranded RNA segments, is spread by infected mosquitoes and causes large viral outbreaks in Africa. RVFV encodes ...a nucleoprotein (N) that encapsidates the viral RNA. The N protein is the major component of the ribonucleoprotein complex and is also required for genomic RNA replication and transcription by the viral polymerase. Here we present the 1.6 Å crystal structure of the RVFV N protein in hexameric form. The ring-shaped hexamers form a functional RNA binding site, as assessed by mutagenesis experiments. Electron microscopy (EM) demonstrates that N in complex with RNA also forms rings in solution, and a single-particle EM reconstruction of a hexameric N-RNA complex is consistent with the crystallographic N hexamers. The ring-like organization of the hexamers in the crystal is stabilized by circular interactions of the N terminus of RVFV N, which forms an extended arm that binds to a hydrophobic pocket in the core domain of an adjacent subunit. The conformation of the N-terminal arm differs from that seen in a previous crystal structure of RVFV, in which it was bound to the hydrophobic pocket in its own core domain. The switch from an intra- to an inter-molecular interaction mode of the N-terminal arm may be a general principle that underlies multimerization and RNA encapsidation by N proteins from Bunyaviridae. Furthermore, slight structural adjustments of the N-terminal arm would allow RVFV N to form smaller or larger ring-shaped oligomers and potentially even a multimer with a super-helical subunit arrangement. Thus, the interaction mode between subunits seen in the crystal structure would allow the formation of filamentous ribonucleocapsids in vivo. Both the RNA binding cleft and the multimerization site of the N protein are promising targets for the development of antiviral drugs.
The threat of a pandemic outbreak of influenza virus A H5N1 has become a major concern worldwide. The nucleoprotein (NP) of the virus binds the RNA genome and acts as a key adaptor between the virus ...and the host cell. It, therefore, plays an important structural and functional role and represents an attractive drug target. Here, we report the 3.3-Å crystal structure of H5N1 NP, which is composed of a head domain, a body domain, and a tail loop. Our structure resolves the important linker segments (residues 397-401, 429-437) that connect the tail loop with the remainder of the molecule and a flexible, basic loop (residues 73-91) located in an arginine-rich groove surrounding Arg150. Using surface plasmon resonance, we found the basic loop and arginine-rich groove, but mostly a protruding element containing Arg174 and Arg175, to be important in RNA binding by NP. We also used our crystal structure to build a ring-shaped assembly of nine NP subunits to model the miniribonucleoprotein particle previously visualized by electron microscopy. Our study of H5N1 NP provides insight into the oligomerization interface and the RNA-binding groove, which are attractive drug targets, and it identifies the epitopes that might be used for universal vaccine development.--Ng, A. K.-L., Zhang, H., Tan, K., Li, Z., Liu, J.-h., Chan, P. K.-S., Li, S.-M., Chan, W.-Y., Au, S. W.-N., Joachimiak, A., Walz, T., Wang, J.-H., Shaw, P.-C. Structure of the influenza virus A H5N1 nucleoprotein: implications for RNA binding, oligomerization, and vaccine design.
Soil-water characteristic curve (SWCC) and soil hydraulic conductivity are important soil hydrodynamic parameters, which are of great significance in production practice. In 128 soil samples ...collected from 0-20, 20-40 cm layers at sampling scales of 32×32 m, the authors determined the effect of different periods of time on SWCC and soil hydraulic conductivity in fields mulched gravel. The water holding capacity of soil changes dramatically throughout time, with NEW having the maximum water holding capacity. As the planting time increases, the soil water holding capacity decreases. Both van Genuchten (VG) and Gardner models can fit SWCC in different time periods well, but the fitting accuracy of the VG model is higher. Geostatistics and Kriging interpolation are used to study the spatial variability of the VG model parameters of the SWCCs. The parameters θs, α, and n show a strong spatial correlation as a whole and are slightly affected by random factors. The changing trend of soil unsaturated hydraulic conductivity (K(θ)) in gravel-mulched fields is faster than the CHECK, and the NEW has the fastest change trend. The data suggest that fields mulched with gravel retain more water, with the NEW having the most noticeable water retention effect. The study’s findings can be used to investigate the regional variations in soil hydrodynamic characteristics in gravel mulched fields in arid locations.
The Interconnected River System Network (IRSN) plays a crucial role in water resource allocation, water ecological restoration and water quality improvement. It has become a key part of the urban ...lake management. An evaluation methodology system for IRSN project can provide important guidance for the selection of different water diversion schemes. However, few if any comprehensive evaluation systems have been developed to evaluate the hydrodynamics and water quality of connected lakes. This study developed a comprehensive evaluation system based on multi-indexes including aspects of water hydrodynamics, water quality and socioeconomics. A two-dimensional (2-D) mathematical hydrodynamics and water quality model was built, using NH
3
-N, TN and TP as water quality index. The IRSN project in Tangxun Lake group was used as a testbed here, and five water diversion schemes were simulated and evaluated. Results showed that the IRSN project can improve the water fluidity and the water quality obviously after a short time of water diversion, while the improvement rates decreased gradually as the water diversion went on. Among these five schemes, Scheme V showed the most noticeable improvement in hydrodynamics and water quality, and brought the most economic benefits. This comprehensive evaluation method can provide useful reference for the implementation of other similar IRSN projects.
Drying could change the microstructure of cement-based materials and inevitably affect their mechanical properties. The isothermal drying process of concrete at three scales and its effect on ...compressive behavior and microstructure were investigated. The deformations of cement paste, mortar, and concrete in the drying process all exhibit the characteristics of expansion first and then shrinkage. The porosity and average pore diameter increase after drying, which is mainly attributed to the increase of pores less than 100 nm diameter for paste and to the pores within 100~1000 nm for mortar. Drying makes paste denser, while the bonding between paste and aggregate is weakened. Microstructural studies indicate that the increase in compressive strength of concrete caused by isothermal drying is the competition result between the strengthening effect and the weakening effect, and is related to the paste content.
Characterization of the variability of soil moisture, salt content and organic matter content (SOM) is of great significance in agricultural production management and sustainable soil utilization. We ...present a case study of the variability and modelling with the depth of soil moisture, salt and SOM in a gravel-sand mulched jujube orchard, using Geostatistics and Kriging interpolation. Soil moisture, salt and SOM were measured in 256 samples collected from a gravel-sand mulched jujube orchard in the 0-10, 10-20, 20-30 and 30-50 cm. Soil moisture, salt and SOM were more variable in the surface soil, due to several environmental factors, the coefficients of variation (CV) of soil were lower than 23%, indicating weak to moderate variation. The coefficient of variation of moisture and organic matter decreased with the depth and the salinity increased with the depth. There is a significant correlation between each soil layer, which decreases with the increase of the soil layer. The accuracy of the function model with depth as an independent variable and soil properties as a dependent variable is higher than 0.88. To master the relationship among soil depth, salinity, soil moisture and organic matter content can provide theoretical value for agricultural comprehensive management.
TRPC4 ion channel was reported to be regulated by small molecular inhibitors and calmodulin. We discuss these findings in the context of other members of TRPC subfamily modulated by different ...stimulants.
Based on the meteorological data from 46 stations in the Hai River Basin (HRB) from 1961-2010, the annual and seasonal variation of reference evapotranspiration was analyzed. The sensitivity ...coefficients combined with the detrend method were used to discuss the dominant factor affecting the reference evapotranspiration (ETo). The obtained results indicate that the annual reference evapotranspiration is dominated by the decreasing trends at the confidence level of 95% in the southern and eastern parts of the HRB. The sensitivity order of climatic variables to ETo from strong to weak is: relativity humidity, temperature, shortwave radiation and wind speed, respectively. However, comprehensively considering the sensitivity and its variation strength, the detrend analysis indicates that the decreasing trends of ETo in eastern and southern HRB may be caused mainly by the decreasing wind speed and shortwave radiation. As for the relationship between human activity and the trend of ETo, we found that ETo decreased more significantly on the plains than in the mountains. By contrast, the population density increased more considerably from 2000 to 2010 on the plains than in the mountains. Therefore, in this paper, the correlation of the spatial variation pattern between ETo and population was further analyzed. The spatial correlation coefficient between population and the trend of ETo is -0.132, while the spatial correlation coefficient between the trend of ETo and elevation, temperature, shortwave radiation and wind speed is 0.667, 0.668, 0.749 and 0.416, respectively. This suggests that human activity has a certain influence on the spatial variation of ETo, while natural factors play a decisive role in the spatial variation of reference evapotranspiration in this area.
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