Long noncoding RNAs (lncRNA) participate in carcinogenesis and tumor progression in lung cancer. Here, we report the identification of a lncRNA signature associated with metastasis of non-small cell ...lung cancer (NSCLC). In particular, elevated expression of LINC00963 (MetaLnc9) in human NSCLC specimens correlated with poor prognosis, promoted migration and invasion of NSCLC cells
, and enhanced lung metastasis formation
Mechanistic investigations showed that MetaLnc9 interacted with the glycolytic kinase PGK1 and prevented its ubiquitination in NSCLC cells, leading to activation of the oncogenic AKT/mTOR signaling pathway. MetaLnc9 also interacted with P54nrb/NonO (NONO) to help mediate the activity of CRTC, a coactivator for the transcription factor CREB, reinforcing a positive feedback loop for metastasis. Taken together, our results establish MetaLnc9 as a driver of metastasis and a candidate therapeutic target for treating advanced NSCLC.
.
Nanofiltration (NF) has been used as pretreatment to reverse osmosis (RO) desalination processes for lowering the required pressure. In this work, a novel kind of amphoteric thin-film composite (TFC) ...NF membranes were prepared through a method of coating and cross-linking using sulfated chitosan (SCS), polysulfone (PS) ultrafiltration (UF) membrane, and epichlorohydrin (ECH) as the active layer material, the base membrane, and the cross-linking agent, respectively. At 0.40MPa and ambient temperature, the rejections of the resultant membrane to Na2SO4 and NaCl solutions (1000mgL−1) were 90.8% and 32.5%, while the permeate fluxes were 22.9 and 58.4kgm−2h−1, respectively. The structure and morphology of the resultant SCS/PS composite NF membranes were characterized with attenuated total reflection infrared spectroscopy (ATR-IR), environmental scanning electron microscopy (ESEM), and atomic force microscopy (AFM). The rejection performances suggested that SCS/PS composite NF membranes cross-linked by ECH have a potential for the separation of mono/divalent inorganic electrolytes from low-molecular-weight organics.
► Thin-film amphoteric composite (TFC) NF membranes. ► Cross-linking of sulfated chitosan (SCS) and epichlorohydrin (ECH). ► Structure and morphology of SCS composite with epichlorohydrin (ECH) NF membranes. ► Separation of mono/divalent inorganic electrolytes from low-molecular-weight organics.
Phosphorous doped carbon dots (P-CDs) are prepared by a one-step hydrothermal method. The phenomenon of aggregation induced red shift emission (AIRSE) is observed in the obtained P-CDs. The emission ...wavelength of a P-CD solution can be tuned in the range from 455 nm to 595 nm by changing the solution concentration.
MicroRNAs (miRNAs) are involved in human cancer including non-small cell lung cancer (NSCLC). In this study, we compared miRNA expression microarray of SPC-A-1sci (high metastatic) and SPC-A-1 ...(weakly metastatic) cells. We found that miRNA-10a was up-regulated in NSCLC compared with corresponding normal tissues. High expression of miR-10a was associated with tumor node metastasis and lymph node metastasis. Furthermore, overexpression of miR-10a promoted NSCLC cell proliferation, migration and invasion in vitro. We found that PTEN was a direct target of miR-10a in NSCLC. Also miR-10a activated the PTEN/AKT/ERK pathway. We suggest that miR-10a contributes to NSCLC by targeting PTEN.
Abstract
Background
KH-type splicing regulatory protein (KHSRP) plays an important role in cancer invasion, but the relevant mechanism is not well known. In the present study, we investigated the ...function and potential molecular mechanism of KHSRP in non-small cell lung cancer (NSCLC) metastasis and elucidated its clinical significance.
Methods
Isobaric tags for relative and absolute quantitation and the SWATH™ approach were combined with nanoliquid chromatography-tandem mass spectrometry analysis to identify metastasis-associated nucleoproteins in NSCLC. Real-time PCR and Western blot were used to screen for metastasis-associated candidate molecules. Gene knockdown and overexpression were used to investigate their functions and molecular mechanisms in lung cancer cells. Coimmunoprecipitation (Co-IP) experiments were performed to identify the interactions between candidate molecules and their interacting proteins. Gene expression and its association with multiple clinicopathologic characteristics were analyzed by immunohistochemistry (IHC) and Western blot in human lung cancer specimens.
Results
KHSRP was identified as a metastasis-associated candidate molecule. In NSCLC cell lines, knockdown of KHSRP significantly reduced lung cancer cell proliferation, migration, and invasion in vitro and in vivo, whereas overexpression of KHSRP did the opposite. Mechanistically, the protein heterogeneous nuclear ribonucleoprotein C (C1/C2) (HNRNPC) was identified to interact with KHSRP using Co-IP experiments. In NSCLC cell lines, overexpression of HNRNPC significantly promoted lung cancer cell proliferation, migration, and invasion in vitro and in vivo. KHSRP and HNRNPC may induce human lung cancer cell invasion and metastasis by activating the IFN-α-JAK-STAT1 signaling pathway. Drastically higher expression levels of KHSRP and HNRNPC were observed in lung cancer tissues compared to those in adjacent noncancerous tissues. Increased KHSRP and HNRNPC expression was significantly associated with advanced tumor stages and metastasis (both lymph node and distant). Kaplan-Meier survival analysis showed that patients with high KHSRP and HNRNPC expression levels were predicted to have the shortest survival times and to have a poor prognosis.
Conclusions
KHSRP plays an important role in NSCLC metastasis and may serve as a potential prognostic marker and novel therapeutic target for lung cancer metastasis treatment.
The catalytic activities of three topotactic phase strontium cobaltites (SCO, hexagonal, orthorhombic and tetragonal) for H
2
O
2
decomposition were studied. All they present excellent catalytic ...activity with good stability at cycled test, and the mesoporous tetragonal SCO exhibits the best activity. Detailed kinetic research indicates the zero-order process of H
2
O
2
decomposition. Increasing the temperature will accelerate the reactions greatly with the controlled temperature in the range of 20–45 °C. The calculated active energy (
E
a
) for H-SCO, O-SCO and T-SCO is 93.2, 85.1 and 87.5 (kJ/mol), respectively. The efficient catalytic degradation of Rhodamine B illustrates that SCO is a kind of promising catalysis for the advanced oxidation process.
Growing evidence indicates that miR-200c is involved in carcinogenesis and tumor progression in non-small-cell lung cancer (NSCLC). However, its precise biological role remains largely elusive.
The ...functions of miR-200c and USP25 in migration/invasion and lung metastasis formation were determined by transwell and tail vein injection assays, respectively. The potential regulatory targets of miR-200c were determined by prediction tools, correlation with target protein expression, and luciferase reporter assay. The mRNA expression levels of miR-200c and USP25 were examined in NSCLC cell lines and patient specimens using quantitative reverse transcription-PCR. The protein expression levels of USP25 were examined in NSCLC cell lines and patient specimens using western blot and immunohistochemical staining.
We demonstrated that over-expression of miR-200c inhibited NSCLC cells migration, invasion, epithelial-mesenchymal transition (EMT) in vitro and lung metastasis formation in vivo. Further studies revealed that USP25 was a downstream target of miR-200c in NSCLC cells as miR-200c bound directly to the 3'-untranslated region of USP25, thus reducing both the messenger RNA and protein levels of USP25. Silencing of the USP25 gene recapitulated the effects of miR-200c over-expression. Clinical analysis indicated that miR-200c was negatively correlated with clinical stage, lymph node metastasis in NSCLC patients. Moreover, USP25 protein and mRNA level expressions were higher in NSCLC patients, compared to healthy control, and correlated with clinical stage and lymphatic node metastasis.
These findings indicate that miR-200c exerts tumor-suppressive effects for NSCLC through the suppression of USP25 expression and suggests a new therapeutic application of miR-200c in the treatment of NSCLC.
Layered Co(OH)
2
has been regarded as a potential candidate for pseudocapacitor electrode material, but it is limited by its poor conductivity and low surface area. Herein, carbonated multi-walled ...carbon nanotubes (MWCNTs-COOH) were used to improve the conductivity of α-Co(OH)
2
nanocomposites. α-Co(OH)
2
nanoflowers were successfully grown and tangled with MWCNTs-COOH to form a randomly oriented network structure mediated by ammonia using water-methanol as co-solvent at room temperature. The capacitance performances of the obtained α-Co(OH)
2
@MWCNT-COOH composites were investigated to find that MWCNT-COOH improved the specific capacitance efficiently. Especially, in the presence of 20% in mole of MWCNT-COOH, the composite gave rise to the high specific capacitance of 1027 F/g at the current density of 2 A/g with good cycle stability, whose specific capacitance remained 78% of its original even after 5000 cycles at 30 A/g current. The large surface area and strengthening the conductivity of the composites lead to the improvement of efficiency in charge/discharge process.
Various emission colors and morphologies of organometal halide perovskite (OHMP) nanocrystals were obtained via solvent-induced precipitation. The nanoplatelets with blue emission were produced in ...the low polar solvents, whreas the increase in polarity of poor solvents results in the red shift of emission of obtained NCs. On the other hand, MaPbBr3 NCs dispersing in organic solvents gives rise to three status, including stable dispersion, dissolving or PL quenching depending on the solvent properties.
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The solvent-induced precipitation in a mixture of good and poor solvents was often used to synthesize organometal halide perovskite nanocrystals (OMHP NCs). Here we investigate the formation of OMHP NCs by using HBr based precursors in various solvents. Various emission colors and morphologies of perovskite NCs were obtained depending on the solvents used. The nanoplatelets with blue emission were produced for the low polar solvents, such as toluene, PhCl and EtOAc. The increase in polarity of poor solvents results in the red shift of emission of obtained NCs. The much more polar solvents like acetone and CH3CN lead to formation of CH3NH3PbBr3 NCs (MaPbBr3) with cubic phase. The dispersion behaviors of MaPbBr3 NCs in different solvents was also investigated. MaPbBr3 NCs can exist stably in most unpolar solvents. However, they will be dissolved in polar solvents, like acetone and acetonitrile, to give rise to a clear solution with red emission of Pb2+. By injecting this clear solution to unpolar solvents, OMHP NCs can be reproduced.
Highlights • Tumor acidification aggravates the activation of HCC-infiltrating HSCs. • The activation of HSCs under acidity is dependent on the phosphorylation of ERK. • Acidity-activated HSCs ...contribute to the metastasis of HCC. • Elevated secreted OPN of acidity-activated HSCs promotes the metastasis of HCC. • OPN shows prognosis significance, combination with HSCs activation marker α-SMA. • Acidic tumor microenvironment represents a novel mechanism for HCC metastasis and provides a potential therapeutic strategy.
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