A novel Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterial strain, DHC34
, was isolated from forest soil of Dinghushan Biosphere Reserve, Guangdong Province, China ...(112° 31' E 23° 10' N). It grew optimally on R2A medium at 28 °C, at pH 6.0-7.0 and in the presence of 0-1 % (w/v) NaCl. Strain DHC34
was closely related to
LMG 28138
(98.5 % 16S rRNA gene sequence similarity). 16S rRNA gene sequence analysis showed that strain DHC34
formed a clade with
LMG 28138
, which is next to but branched deeply with
ICMP 2807
. The phylogenetic relationships among these three strains were also supported with the phylogram based on concatenated partial
B,
A and
B gene sequences. The phylogenomic tree generated with the UBCG tool showed that strains DHC34
and
ICMP 2807
were in a different clade. The DNA-DNA relatedness values between strain DHC34
and
LMG 28138
and
ICMP 2807
were much lower than 70 %. Strain DHC34
contained ubiquinone 8 as the major respiratory quinone. Its major fatty acids were C
, C
cyclo and C
cyclo ω8
. The DNA G+C content of strain DHC34
was 64.2 mol%. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, three unidentified aminophospholipids, four unidentified phospholipids, one unidentified aminolipid and a polar lipid. The phenotypic, phylogenetic, genotypic and chemotaxonomic data showed that strain DHC34
represents a novel species of a new genus in the family
, for which the name
gen. nov., sp. nov. is proposed. The type strain of
is DHC34
(=KCTC 42628
=LMG 28845
). On the basis of the current data,
is renamed as
comb. nov.
The fast‐growing amount of data that is produced every year creates an urgent need for ultracapacity storage media. However, 2D spatial resolution in the conventional optical data storage media has ...almost reached the limit. Further enlargement of storage capacity may rely on the development of the next‐generation data storage materials containing multiplexed information dimensions. Herein, a series of novel deep‐trap persistent luminescence materials (Sr1‐xBax)Si2O2N2:Eu/Yb,Dy with multicolor emissions in the whole visible region is developed and demonstrated a bit‐by‐bit optical data storage and readout strategy based on photon trapping and detrapping processes in these materials. Optical data can be handily encoded on a flexible film by a commercially available 405 nm laser and decoded by heating or by 980 nm laser scanning. The decoded information contains tunable spectral characteristics, which allows for the emission–intensity–multiplexing or emission–wavelength–multiplexing. The storage and readout strategy not only shows a great promise in the application of multidimensional rewritable optical data storage, but also opens new opportunities for advanced display technology and information security system.
A bit‐by‐bit optical data storage and readout strategy based on photon trapping and detrapping processes in deep‐trap persistent luminescence materials is demonstrated in this study. The readout information contains tunable spectral characteristics, allowing for the emission–intensity–multiplexing or emission–wavelength–multiplexing, which may greatly enlarge the storage capacity in multidimensional data storage systems.
To investigate the effect of different thawing methods on the quality of frozen cuttlefish, six different thawing methods were used: hydrostatic thawing (HT), flowing water thawing (FWT), saline ...solution thawing (SWT), ultrasonic water thawing (UWT), microwave thawing (MT), and 4°C refrigerator thawing (RT). In this study, the water retention (thawing loss rate, centrifugal loss rate, and cooking loss), pH value, malondialdehyde content, TVB-N value, and sulfhydryl content were measured to evaluate the quality after thawing. Protein secondary structure was measured using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, protein tertiary structure was obtained by fluorescence spectroscopy and water migration was determined using low-field NMR spectroscopy. The results showed that the microwave thawing time was the shortest, but the water holding capacity after thawing was the worst, and the TVB-N content was the highest at 14.31 mg/100 g. Ultrasonic thawing led to the best water holding capacity, but ultrasound promoted the oxidation of protein and fat. Microscopic observation showed that the muscle fiber bundles in the saline solution thawing samples were compactly arranged and intact, with minimal gaps. The samples thawed in saline solution had the highest hardness and chewiness. The results of comprehensive analysis showed that saline solution thawing and ultrasonic water thawing are more suitable for thawing cuttlefish, and this study provides some theoretical basis for the selection of thawing methods in the actual production of cuttlefish.
Epigallocatechin gallate (EGCG) is associated with various health benefits. In this review, we searched current work about the effects of EGCG and its wound dressings on skin for wound healing. ...Hydrogels, nanoparticles, micro/nanofiber networks and microneedles are the major types of EGCG-containing wound dressings. The beneficial effects of EGCG and its wound dressings at different stages of skin wound healing (hemostasis, inflammation, proliferation and tissue remodeling) were summarized based on the underlying mechanisms of antioxidant, anti-inflammatory, antimicrobial, angiogenesis and antifibrotic properties. This review expatiates on the rationale of using EGCG to promote skin wound healing and prevent scar formation, which provides a future clinical application direction of EGCG.
Selective cleavage and functionalization of C−C bonds have important applications in organic synthesis and biomass utilization. However, functionalization of C−C bonds by controlled cleavage remains ...difficult and challenging because they are inert. Herein, we describe an unprecedented efficient protocol for the breaking of successive C−C bonds in alcohols to form esters with one or multiple carbon atoms less using heterogeneous cobalt nanoparticles as catalyst with dioxygen as the oxidant. A wide range of alcohols including inactive long‐chain alkyl aryl alcohols undergo smoothly successive cleavage of adjacent −(C−C)n− bonds to afford the corresponding esters. The catalyst was used for seven times without any decrease in activity. Characterization and control experiments disclose that cobalt nanoparticles are responsible for the successive cleavage of C−C bonds to achieve excellent catalytic activity, while the presence of Co‐Nx has just the opposite effect. Preliminary mechanistic studies reveal that a tandem sequence reaction is involved in this process.
Cobalt nanoparticles‐catalyzed aerobic oxidative cleavage of adjacent −(C−C)n− bonds in succession in a wide range of alcohols as well as inactive long‐chain alkyl aryl alcohols to afford esters was developed. Control experiments disclose that metallic cobalt nanoparticles are responsible for the outstanding catalytic performance rather than single atom cobalt.
The evolution of oxygenic photosynthesis is a pivotal event in Earth's history because the O
released fundamentally changed the planet's redox state and facilitated the emergence of multicellular ...life. An intriguing hypothesis proposes that hydrogen peroxide (H
O
) once acted as the electron donor prior to the evolution of oxygenic photosynthesis, but its abundance during the Archean would have been limited. Here, we report a previously unrecognized abiotic pathway for Archean H
O
production that involves the abrasion of quartz surfaces and the subsequent generation of surface-bound radicals that can efficiently oxidize H
O to H
O
and O
. We propose that in turbulent subaqueous environments, such as rivers, estuaries and deltas, this process could have provided a sufficient H
O
source that led to the generation of biogenic O
, creating an evolutionary impetus for the origin of oxygenic photosynthesis.
PCBP1 is a multifunctional RNA-binding protein (RBP) expressed in most human cells and is involved in posttranscriptional gene regulation. PCBP1 regulates the alternative splicing, translation and ...RNA stability of many cancer-related genes and has been identified as a potential tumour suppressor gene. PCBP1 inhibits the invasion of hepatocellular carcinoma (HCC) cells, but there are few studies on the specific regulatory target and mechanism of RBPs in HCC, and it is unclear whether PCBP1 plays a role in tumour metastasis as a splicing factor. We analysed the regulation of gene expression by PCBP1 at the transcriptional level. We obtained and analysed PCBP1-knockdown RNA-seq data and eCLIP-seq data of PCBP1 in HepG2 cells and found that PCBP1 widely regulates the alternative splicing and expression of genes enriched in cancer-related pathways, including extracellular matrix, cell adhesion, small molecule metabolic process and apoptosis. We validated five regulated alternative splicing events affected by PCBP1 using RT-qPCR and found that there was a significant difference in the expression of APOC1 and SPHK1 between tumour and normal tissues. In this study, we provided convincing evidence that human PCBP1 profoundly regulates the splicing of genes associated with tumour metastasis. These findings provide new insight into potential markers or therapeutic targets for HCC treatment.
CsPbBr3 perovskite quantum dots (PQDs)/ethylene vinyl acetate (EVA) composite films were prepared via a one-step method; on the basis of this, both supersaturated recrystallization of CsPbBr3 PQDs ...and dissolution of EVA were realized in toluene. The prepared films display outstanding green-emitting performance with high color purity of 92% and photoluminescence (PL) quantum yield of 40.5% at appropriate CsPbBr3 PQD loading. They possess long-term stable luminescent properties in the air and in water, benefiting from the effective protection of CsPbBr3 PQDs by the EVA matrix. Besides, the prepared CsPbBr3 PQDs/EVA films are flexible enough to be repeatedly bent for 1000 cycles while keeping unchanged the PL intensity. The optical properties of the CsPbBr3 PQDs/EVA films in white light-emitting diodes were also studied by experiments and theoretical simulation. Overall, facile preparation process, good long-term stability, and high flexibility allow our green-emitting CsPbBr3 PQDs/EVA films to be applied in lighting applications and flexible displays.
In this work, Pt/reduced graphene oxide nanocomposite (Pt/RGO) was prepared by in situ chemical reduction. The Pt/RGO modified glassy carbon electrode exhibited excellent catalytic activity towards ...the oxidation of dopamine (DA) and uric acid (UA) in the presence of ascorbic acid (AA). Differential pulse voltammetry (DPV) was used to simultaneous determination of DA and UA with high sensitivity.
•Pt/reduced graphene oxide nanocomposite was prepared by in situ chemical reduction.•Pt nanoparticles were well-dispersed on reduced graphene oxide sheets.•The as-prepared nanocomposite displayed high electrocatalytic activity.•It was used to simultaneous determination of dopamine and uric acid in the presence of ascorbic acid with high sensitivity.
In this paper, a Pt/reduced graphene oxide (Pt/RGO) modified glassy carbon electrode was prepared for the detection of dopamine (DA) and uric acid (UA) in the presence of high concentration of ascorbic acid (AA). The electrochemical behavior of the Pt/RGO modified electrode was characterized by cyclic voltammetry and differential pulse voltammetry, which showed good performance toward individual detection of DA and UA and even their simultaneous detection in the presence of 1.0mM AA. Evidently, the electro-oxidation peak currents displayed linear relationship with the associated DA and UA concentrations in the range of 10.0-170.0 μM and 10.0-130.0 μM, respectively, with the detection limits of 0.25 μM for DA and 0.45 μM for UA at three folds of the signal-to-noise ratio. The good performance of the Pt/RGO modified electrode provided a promising alternative in routine sensing applications.
Synergistic regulations among multiple microRNAs (miRNAs) are important to understand the mechanisms of complex post-transcriptional regulations in humans. Complex diseases are affected by several ...miRNAs rather than a single miRNA. So, it is a challenge to identify miRNA synergism and thereby further determine miRNA functions at a system-wide level and investigate disease miRNA features in the miRNA-miRNA synergistic network from a new view. Here, we constructed a miRNA-miRNA functional synergistic network (MFSN) via co-regulating functional modules that have three features: common targets of corresponding miRNA pairs, enriched in the same gene ontology category and close proximity in the protein interaction network. Predicted miRNA synergism is validated by significantly high co-expression of functional modules and significantly negative regulation to functional modules. We found that the MFSN exhibits a scale free, small world and modular architecture. Furthermore, the topological features of disease miRNAs in the MFSN are distinct from non-disease miRNAs. They have more synergism, indicating their higher complexity of functions and are the global central cores of the MFSN. In addition, miRNAs associated with the same disease are close to each other. The structure of the MFSN and the features of disease miRNAs are validated to be robust using different miRNA target data sets.
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