Dye and nitro-compound pollution has become a significant issue worldwide. The adsorption and degradation of dyes and nitro-compounds have recently become important areas of study. Different methods, ...such as precipitation, flocculation, ultra-filtration, ion exchange, coagulation, and electro-catalytic degradation have been adopted for the adsorption and degradation of these organic pollutants. Apart from these methods, adsorption, photocatalytic degradation, and chemical degradation are considered the most economical and efficient to control water pollution from dyes and nitro-compounds. In this review, different kinds of dyes and nitro-compounds, and their adverse effects on aquatic organisms and human beings, were summarized in depth. This review article covers the comprehensive analysis of the adsorption of dyes over different materials (porous polymer, carbon-based materials, clay-based materials, layer double hydroxides, metal-organic frameworks, and biosorbents). The mechanism and kinetics of dye adsorption were the central parts of this study. The structures of all the materials mentioned above were discussed, along with their main functional groups responsible for dye adsorption. Removal and degradation methods, such as adsorption, photocatalytic degradation, and chemical degradation of dyes and nitro-compounds were also the main aim of this review article, as well as the materials used for such degradation. The mechanisms of photocatalytic and chemical degradation were also explained comprehensively. Different factors responsible for adsorption, photocatalytic degradation, and chemical degradation were also highlighted. Advantages and disadvantages, as well as economic cost, were also discussed briefly. This review will be beneficial for the reader as it covers all aspects of dye adsorption and the degradation of dyes and nitro-compounds. Future aspects and shortcomings were also part of this review article. There are several review articles on all these topics, but such a comprehensive study has not been performed so far in the literature.
Cadmium is a common environmental heavy metal pollutant that can accumulate over long periods of time and cause disease. Thus, analysis of the molecular mechanisms affected by cadmium in the body ...could be of great significance for the prevention and treatment of cadmium-related diseases. In this study, flow cytometry, immunofluorescence, transmission electron microscopy, H&E (Hematoxylin Eosin) staining and TUNEL (TdT-mediated dUTP Nick-End Labeling) assays were used to verify that cadmium induced apoptosis and immune responses in bovine mammary epithelial cells (BMECs) and in mouse mammary gland. Isolated BMECs cultured with or without cadmium were collected to screen miRNA (microRNA) using high-throughput sequencing. There were 42 differentially-expressed miRNAs among which 27 were upregulated and 15 downregulated including bta-miR-133a, bta-miR-23b-5p, bta-miR-29e, bta-miR-365–5p, bta-miR-615, bta-miR-7, bta-miR-11975, bta-miR-127, and bta-miR-411a. Among those, miR-133a (which can specifically target TGFB2 (Recombinant Transforming Growth Factor Beta 2) was the most significantly downregulated with a fold-change of 5.27 in BMECs cultured with cadmium. Application of the double luciferase reporter system, western blotting, and qRT-PCR (Quantitative Real-time PCR) revealed that circ08409 can directly bind to miR-133a. Experiments demonstrated that circRNA-08409 could adsorb bta-miR-133a. Both circ08409 and TGFB2 significantly increased apoptosis and altered expression level of a series of inflammatory factors in BMECs. In contrast, miR-133a decreased significantly apoptosis and inflammation in the cells. Compared with cultures receiving only cadmium, the miR-133a+cadmium cultures exhibited significant reductions in the occurrence of late apoptosis. Overall, results indicated that circ08409 could relieve the inhibitory effect of miR-133a on TGFB2 expression by combining with miR-133a and subsequently modulating cell proliferation, apoptosis and inflammation. Overall, the data suggested that the circ08409/miR-133a/TGFB2 axis might play a role in mediating the effect of cadmium on BMECs. As such, data provide novel insights into controlling hazards that cadmium could induce in the mammary gland.
Cadmium promotes apoptosis and inflammation via the circ08409/miR-133a/TGFB2 Display omitted
•Cadmium can induce apoptosis and can interfere with the cell cycle at a certain dose.•High-throughput sequencing of miRNA in blank group and Cadmium treatment group.•Cadmium leads to apoptosis and inflammation in BMECs via the circ08409/miR-133a/TGFB2 axis.
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•Whole crop corn was inoculated with various exogenous epiphytic microbiota.•Napier grass microbiota caused high pH and acetic acid concentrations.•Final silage of whole crop corn ...microbiota had a broad microbial diversity.•Terminal silage of Sudan grass microbiota develop undesirable bacterial genera.•Forage sorghum microbiota adopted well and improved silage fermentation quality.
This study analyzed the feasibility and effects of exogenous epiphytic microbiota on the fermentation quality and microbial community of whole crop corn. Gamma irradiated whole crop corn was ensiled with distilled water (STR), extracted microbiota of whole crop corn (WCC), Napier grass (NAP), forage sorghum (FS) or Sudan grass (SUD). WCC significantly increased LA concentration and decreased the pH during early ensiling. FS had significantly higher LA and lower pH during terminal ensiling. NAP caused higher pH and AA followed by the SUD silage. During 3 d of ensiling WCC and FS silage was dominated by Lactobacillus and Lactococcus while Weissella dominated NAP and SUD silage. Terminal silage was dominated by Lactobacillus in FS and Acinetobacter in NAP while Lactobacillus, Acetobacter, Acinetobacter and Sphingobium dominated WCC and SUD silage. The study demonstrated that FS microbiota transplantation positively influenced the microbial community and fermentation quality of whole crop corn silage as compared to other microbiota.
The 'war on terror' has not only increased the operation and significance of Islamic parties but also intensified suspicion against them. The lack of comprehensive theoretical research about Islamic ...parties has further contributed to misunderstanding of their nature and politics. This article theorizes and classifies Islamic parties, especially of Pakistan, and explores their origin, development and nature. The conventional theories of party origin and traditional classifications of political parties are shown to have limited validity in the case of Islamic parties. Rather, the origin and development of Islamic parties is explored through the theoretical construct of historical crisis situation theory, consisting of four crisis situations. Moreover, a new typology of Islamic parties is developed and the Islamic parties are classified on the basis of six variables into three parties: clerical, conservative and Islamist. The exploration of the role of Islamic parties in Pakistan shows that party variables are not only essential to understand their proper nature but also critical to comprehend their politics. Though unanimously categorized as 'Islamic', their politics significantly differ and Pakistan provides a typical case to show the heterogeneous Islamic politics of Islamic parties.
Napier grass (
Pennisetum purpureum
) is well-known due to its high biomass production. The epiphytic microbiota was prepared from Napier, alfalfa, and red clover grass and served as an inoculum. The ...chopped sterilized Napier grass was inoculated with reconstituted epiphytic microbiota, and treatments were designed as: distilled water (N0); Napier grass epiphytic microbiota (NP); alfalfa epiphytic microbiota (AL); and red clover epiphytic microbiota (RC). The results reveal that the reconstituted epiphytic microbiota bacteria efficiently adapted in Napier grass silage, improved fermentation, and produced lactic acid. The alfalfa-grass inoculum rapidly dropped pH and enhanced the lactic acid (LA) and the ratio of lactic acid-to-acetic acid (LA/AA) during the entire ensiling process. However, red clover attains high lactic acid, while Napier grass produces high acetic acid-type fermentation at terminal silage. After day 60 of ensiling,
Lactobacillus
proportion was higher in AL (85.45%), and RC (59.44%), inocula as compared with NP (36.41%), inoculum. The NP inoculum terminal silage was diverse than AL and RC inocula and dominated by
Enterobacter
(16.32%) and
Enterobacteriacea
e (10.16%) and also significantly (
p
< 0.05) higher in acetic acid
.
The present study concluded that AL and RC epiphytic microbiota successfully develop and more efficient than Napier grass microbiota. It is suggested that abundant microbiota isolate from alfala and red clover and develop more economical and efficient inocula for quality fermentation of Napier grass silage in practice.
This study aimed to evaluate the effects of exogenous epiphytic microbiota inoculation on the fermentation quality and microbial community of sudan grass silage. Gamma irradiated sudan grass was ...ensiled with distilled water (STR), epiphytic microbiota of sudan grass (SUDm), forage sorghum (FSm), napier grass (NAPm) and whole crop corn (WCCm). The FSm inoculated silage have significantly lower lactic acid (LA) concentration and higher pH during early ensiling, while LA concentration gradually and significantly increased with the progression of ensiling and have lower pH in relation to other treatments for terminal silage. Inoculation of NAPm resulted in lower LA and higher acetic acid (AA) concentrations, higher pH, ammonia-N and dry matter losses for terminal silage, followed by SUDm silage. Inoculations of WCCm significantly increased LA production and pH decline during early ensiling and have higher LA and pH then NAPm and SUDm silages during final ensiling. The early fermentation of SUDm silage was dominated by genus of
Pediococcus.
The genera of
Lactobacillus
were predominant in WCCm and NAPm silages during 3 days of ensiling, while
Weissella
dominated initial microbial community of FS silage
.
The terminal silage of NAPm was dominated by
Enterobacter
and
Rosenbergiella,
while
Enterobacter
and
Lactobacillus
dominated terminal SUDm silage. The final silage of FSm was dominated by
Lactobacillus, Weissella
and
Pediococcus,
while
Lactobacillus
and
Acetobacter
dominated terminal WCCm silages
.
The results demonstrated that among the four forages the epiphytic microbiota from forage sorghum positively influenced the microbial community and fermentability of sudan grass silage.
Nutrient metabolism is required to maintain energy balance in animal organisms, and fatty acids play an irreplaceable role in fat metabolism. In this study, microRNA sequencing was performed on ...mammary gland tissues collected from cows during early, peak, and late lactation to determine miRNA expression profiles. Differentially expressed miRNA (miR-497) was selected for functional studies of fatty acid substitution. Simulants of miR-497 impaired fat metabolism triacylglycerol (TAG) and cholesterol, whereas knockdown of miR-497 promoted fat metabolism in bovine mammary epithelial cells (BMECs) in vitro. In addition, in vitro experiments on BMECs showed that miR-497 could down-regulate C16:1, C17:1, C18:1, and C20:1 as well as long-chain polyunsaturated fats. Thus, these data expand the discovery of a critical role for miR-497 in mediating adipocyte differentiation. Through bioinformatics analysis and further validation, we identified large tumor suppressor kinase 1 (
) as a target of miR-497. siRNA-
increased concentrations of fatty acids, TAG, and cholesterol in cells, indicating an active role of
in milk fat metabolism. In summary, miR-497/
can regulate the biological processes associated with TAG, cholesterol, and unsaturated fatty acid synthesis in cells, providing an experimental basis for further elucidating the mechanistic regulation of lipid metabolism in BMECs.
The aim of the experiment was to evaluate the potential of promising summer maize genotypes and optimal stage of harvesting these genotypes for ensiling in terms of dry matter (DM), starch, and crude ...protein (CP) yields, silage fermentation quality, nutrients profile, total digestible nutrients, metabolizable energy (ME) content, Cornell Net Carbohydrate and Protein System (CNCPS) carbohydrate (CHO) subfractions composition, in vitro DM digestibility (DMD) and in situ starch degradation characteristics. Six maize genotypes were chosen for the study: DK9108 from Monsanto, P30Y87, P3939 from Pioneer, QPM-300 (quality protein maize) and W94 from the International Maize and Wheat Improvement Center (CIMMYT), and a local cultivar, Afgoii, from the Cereal Research Institute (Persabaq, KP). A total of 72 plots (8 m × 10 m) were blocked in three replicate fields, and within each field, each genotype was sown in four replicate plots according to a randomized complete block design. For the data analysis, the Proc-Mixed procedure of Statistical Analysis System with repeated measure analysis of variance was used. The DM yield was strongly influenced (P < 0.001) by maize genotypes, varying from 12.6 to 17.0 tons/ha. Except for total CHO and ammonia nitrogen (NH
-N), the contents of all measured chemical components varied (P < 0.001) among the genotypes. Further comparison revealed that, genotype P3939 had a higher (P < 0.05) content of CP (7.27 vs. 6.92%), starch (36.7 vs. 27.9%), DMD (65.4 vs. 60.0%), ME (2.51 vs. 2.30 Mcal/kg) and lactic acid (5.32 vs. 4.83%) and lowest content of NDF (37.3 vs. 43.1%), pH (3.7 vs. 4.10) compared to the local cultivar (Afgoii). Advancement of post-flowering maturity from 25 to 35% DM (23 to 41 days after flowering (DAF)) increased (P < 0.05) the DM yield (10.4 to 17.8 tons/ha), starch content (29.1 to 35.0%), DMD (65.3 to 67.3%) and ME (2.34 to 2.47 Mcal/kg), and decreased (P < 0.001) the contents of CP (7.42-6.73%), NDF (48.8-38.5%), pH (4.10 to 3.60), NH
-N (8.93-7.80%N) and effective degradability of starch (95.4 to 89.4). Results showed that for higher yields and silage nutritional and fermentation quality, maize crops should be harvested at whole crop DM content of 30-35% (34 to 41 DAF). It was further concluded that genotype P3939 is the most suitable summer maize genotype for silage production in terms of yields and silage nutritional and fermentation quality under the hot environmental conditions of the tropics.
Reproduction is an important production activity for dairy cows, and their reproductive performance can directly affect the level of farmers' income. To better understand the genomic regions and ...biological pathways of reproduction-related traits of dairy cows, in the present study, three body shape traits-Loin Strength (LS), Rump Angle (RA), and Pin Width (PW)-were selected as indicators of the reproductive ability of cows, and we conducted genome-wide association analyses on them. The heritability of these three traits was medium, ranging from 0.20 to 0.38. A total of 11 significant single-nucleotide polymorphisms (SNPs) were detected associated with these three traits. Bioinformatics analysis was performed on genes close to the significant SNPs (within 200 Kb) of LS, RA, and PW, and we found that these genes were totally enriched in 20 gene ontology terms and six KEGG signaling pathways. Finally, the five genes
,
,
,
, and
were selected as candidate genes that might affect LS. The six genes
,
,
,
,
, and
were selected as candidate genes that might affect RA. The five genes
,
,
,
, and
were selected as candidate genes that might affect the PW of Chinese Holstein cows. Our results could provide useful biological information for the improvement of body shape traits and contribute to the genomic selection of Chinese Holstein cows.
Udder conformation traits are one of the most economic traits in dairy cows, greatly affecting animal health, milk production, and producer profitability in the dairy industry. Genetic analysis of ...udder structure and scores have been developed in Holstein cattle. In our research, we conducted a genome-wide association study for five udder traits, including anterior udder attachment (AUA), central suspensory ligament (CSL), posterior udder attachment height (PUAH), posterior udder attachment width (PUAW), and udder depth (UD), in which the fixed and random model circulating probability unification (FarmCPU) model was applied for the association analysis. The heritability and the standard errors of these five udder traits ranged from 0.04 ± 0.00 to 0.49 ± 0.03. Phenotype data were measured from 1000 Holstein cows, and the GeneSeek Genomic Profiler (GGP) Bovine 100 K SNP chip was used to analyze genotypic data in Holstein cattle. For GWAS analysis, 984 individual cows and 84,407 single-nucleotide polymorphisms (SNPs) remained after quality control; a total of 18 SNPs were found at the GW significant threshold (p < 5.90 × 10−7). Many candidate genes were identified within 200kb upstream or downstream of the significant SNPs, which include MGST1, MGST2, MTUS1, PRKN, STXBP6, GRID2, E2F8, CDH11, FOXP1, SLF1, TMEM117, SBF2, GC, ADGRB3, and GCLC. Pathway analysis revealed that 58 Gene Ontology (GO) terms and 18 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were enriched with adjusted p values, and these GO terms and the KEGG pathway analysis were associated with biological information, metabolism, hormonal growth, and development processes. These results could give valuable biological information for the genetic architecture of udder conformation traits in dairy Holstein cattle.
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