The use of fertilizer results in tall rice plants that are susceptible to lodging and results in reduced plant yields. In this study, using chromosome segment substitution lines, we identified an ...effective quantitative trait loci (QTL) for culm strength, which was designated STRONG CULM2 (SCM2). Positional cloning of the gene revealed that SCM2 was identical to ABERRANT PANICLE ORGANIZATION1 (APO1), a gene previously reported to control panicle structure. A near-isogenic line carrying SCM2 showed enhanced culm strength and increased spikelet number because of the pleiotropic effects of the gene. Although SCM2 is a gain-of-function mutant of APO1, it does not have the negative effects reported for APO1 overexpression mutants, such as decreased panicle number and abnormal spikelet morphology. The identification of lodging-resistance genes by QTL analysis combined with positional cloning is a useful approach for improving lodging resistance and overall productivity in rice.
Sheath blight, caused by Rhizoctonia solani, is one of the most serious diseases of rice. Among 33 rice accessions, mainly from National Institute of Agrobiological Sciences (NIAS) Core Collection, ...we found three landraces from the Himalayas—Jarjan, Nepal 555 and Nepal 8—with resistance to sheath blight in 3 years’ field testing. Backcrossed inbred lines (BILs) derived from a cross between Jarjan and the leading Japanese cultivar Koshihikari were used in QTL analyses. Since later-heading lines show fewer lesions, we used only earlier-heading BILs to avoid association with heading date. We detected eight QTLs; the Jarjan allele of three of these increased resistance. Only one QTL, on chromosome 9 (between markers Nag08KK18184 and Nag08KK18871), was detected in all 3 years. Chromosome segment substitution lines (CSSLs) carrying it showed resistance in field tests. Thirty F2 lines derived from a cross between Koshihikari and one CSSL supported the QTL.
Rice (Oryza sativa L.) can produce black grains as well as white. In black rice, the pericarp of the grain accumulates anthocyanin, which has antioxidant activity and is beneficial to human health. ...We developed a black rice introgression line in the genetic background of Oryza sativa L. ‘Koshihikari’, which is a leading variety in Japan. We used Oryza sativa L. ‘Hong Xie Nuo’ as the donor parent and backcrossed with ‘Koshihikari’ four times, resulting in a near isogenic line (NIL) for black grains. A whole genome survey of the introgression line using DNA markers suggested that three regions, on chromosomes 1, 3 and 4 are associated with black pigmentation. The locus on chromosome 3 has not been identified previously. A mapping analysis with 546 F2 plants derived from a cross between the black rice NIL and ‘Koshihikari’ was evaluated. The results indicated that all three loci are essential for black pigmentation. We named these loci Kala1, Kala3 and Kala4. The black rice NIL was evaluated for eating quality and general agronomic traits. The eating quality was greatly superior to that of ‘Okunomurasaki’, an existing black rice variety. The isogenicity of the black rice NIL to ‘Koshihikari’ was very high.
The appearance of brown rice grown under high temperature conditions is an important characteristic for improvement in Japanese rice breeding programs. We performed a QTL analysis of the appearance ...quality of brown rice using chromosome segment substitution lines of the indica cultivar ‘Habataki’ in the ‘Koshihikari’ genetic background. A line carrying a ‘Habataki’ segment on chromosome 7 showed a high percentage of perfect grains produced under high temperature conditions during the ripening period. To verify the role of this segment, and to narrow down the region containing the useful allele, substitution mapping was performed using multiple paired lines. As a result, the chromosomal location of a gene that we named Appearance quality of brown rice 1 (Apq1) was delimited to a 48-kb region. In addition, we developed an Apq1-near isogenic line (NIL) to evaluate the effect of Apq1 on various agronomic traits. Under high temperature conditions during the ripening period, the Apq1-NIL produced significantly higher percentages of perfect grains than ‘Koshihikari’. Other agronomic traits, including yield and palatability, were similar between the Apq1-NIL and ‘Koshihikari’. Therefore, the ‘Habataki’ allele of Apq1 will be useful in breeding programs aimed at improving the quality of grains ripened under high temperature conditions.
The tolerance to high temperature during the treatment for disinfection in rice seeds is strengthened by reducing their water contents to below 10% before the hot water disinfection treatment. ...Pre-drying treatment prevents the reduction in germinability of the seeds disinfected with hot water at 65ºC for 10 minutes, which is a method used to effectively control several seed-borne diseases. We examined the conditions for pre-drying of seeds using a heating dryer for improving the germinability after the disinfection treatment. The seeds of Nipponbare were dried at 40 – 60ºC for 2 – 72 hours. A higher temperature was more effective for drying. When the seeds were dried at 40ºC, 12 hours of treatment was required to reduce the water content to below 10%. Thereafter, the water content rapidly decreased to around 8% by further heating, but the speed of decrease dropped and the values remained above 7% in the seeds dried for 24 hours at 50ºC. The germinability was not changed by further decrease in water content to less than 7%. In some cases, the seeds dried at 60ºC for 72 hours had lower germinability. Moreover, experimental results using Koshihikari showed that the seeds with a water content reduced to below 9.5% by pre-drying had a germination rate of more than 90%, even after the seeds were disinfected with hot water at 72ºC for 10 min. Taken together, we concluded that pre-drying to a water content of 7 – 9.5% in seeds at 40 – 50ºC for 12 – 24 hours is the most useful method for pre-drying the seeds of Nipponbare and Koshihikari before the hot-water disinfection treatment.
Characterization of resistance genes is essential for the development of a new variety resistant to blast (Magnaporthe grisea) in rice (Oryza sativa L.). In this study, we found that an indica ...variety, Kasalath, harbored a resistance gene that showed a broad spectrum of resistance reactions against standard differential blast isolates from the Philippines and Japan. Quantitative trait locus (QTL) analysis was conducted using backcross inbred lines derived from crosses between Kasalath and the japonica cultivar, Koshihikari, to detect the QTL for blast resistance. QTLs were detected on chromosomes 6 and 8. Further, QTL analysis of chromosome segment substitution lines of Kasalath in the Koshihikari genetic background revealed that the Kasalath allele at the QTL on chromosome 6 strongly contributed to blast resistance. These results suggested that the gene locus might be identical to Pi13(t) that had already been reported. By marker-assisted breeding, a near-isogenic line, Koshihikari Toyama BL7, with a Kasalath chromosome segment containing Pi13(t) introduced into Koshihikari genetic background, was developed. The reaction of Koshihikari Toyama BL7 to the differential blast isolates indicated that Pi13(t) was a broad-spectrum and moderate resistance gene. Since Pi13(t) showed single-gene segregation and was characterized, the gene was re-designated as Pi13.