The development, optimization, and assessment of new methods for the preparation of conjugated materials is key to the continued progress of organic electronics. Direct C–H activation methods have ...emerged and developed over the last 10 years to become an invaluable synthetic tool for the preparation of conjugated polymers for both redox-active and solid-state applications. Here, we evaluate direct (hetero)arylation polymerization (DHAP) methods for the synthesis of indaceno1,2-b:5,6-b′dithiophene-based polymers. We demonstrate, using a range of techniques, including direct visualization of individual polymer chains via high-resolution scanning tunneling microscopy, that DHAP can produce polymers with a high degree of regularity and purity that subsequently perform in organic thin-film transistors comparably to those made by other cross-coupling polymerizations that require increased synthetic complexity. Ultimately, this work results in an improved atom economy by reducing the number of synthetic steps to access high-performance molecular and polymeric materials.
In the central nervous system (CNS), the crosstalk between neural cells is mediated by extracellular mechanisms, including brain-derived extracellular vesicles (bdEVs). To study endogenous ...communication across the brain and periphery, we explored Cre-mediated DNA recombination to permanently record the functional uptake of bdEVs cargo over time. To elucidate functional cargo transfer within the brain at physiological levels, we promoted the continuous secretion of physiological levels of neural bdEVs containing Cre mRNA from a localized region in the brain by in situ lentiviral transduction of the striatum of Flox-tdTomato Ai9 mice reporter of Cre activity. Our approach efficiently detected in vivo transfer of functional events mediated by physiological levels of endogenous bdEVs throughout the brain. Remarkably, a spatial gradient of persistent tdTomato expression was observed along the whole brain, exhibiting an increment of more than 10-fold over 4 months. Moreover, bdEVs containing Cre mRNA were detected in the bloodstream and extracted from brain tissue to further confirm their functional delivery of Cre mRNA in a novel and highly sensitive Nanoluc reporter system. Overall, we report a sensitive method to track bdEV transfer at physiological levels, which will shed light on the role of bdEVs in neural communication within the brain and beyond.
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Breyne, de Almeida and colleagues report a sensitive strategy to detect functional transfer of EVs in the brain. A striatal source of EVs carrying Cre mRNA was generated to monitor the physiological distribution of EVs within the brain. The approach achieved high sensitivity by evaluating permanent DNA recombination throughout the whole brain.
Graphene Formation by Decomposition of C 60 Perdigão, Luís M.A.; Sabki, Syarifah N.; Garfitt, Jason M. ...
Journal of physical chemistry. C,
04/2011, Volume:
115, Issue:
15
Journal Article
The paper addresses the seismic vulnerability assessment of a multi-span footbridge, prone to span unseating due to shorter seat lengths. The structure is representative of a series of pedestrian ...crossings located in the Southern part of Portugal, a region with a relevant seismicity. A probabilistic approach allows considering the variability of the seismic action and uncertainties in the definition of the material properties and/or structural behavior. Based on incremental dynamic analyses and corresponding fragility curves, it is shown that, for code compliance design acceleration, there is a significant probability that the structure will only suffer minor damage.
The adsorption of perylene-3,4,9,10-tetracarboxylic-3,4,9,10-diimide (PTCDI) on Au(1
1
1) has been studied using synchrotron-based X-ray photoelectron spectroscopy and in situ scanning tunnelling ...microscopy. Direct topographic and surface coverage information provided by the scanning probe measurements have enabled us to correlate peaks in the relatively complex carbon core-level photoemission to interactions of the surface with different parts of the PTCDI molecule. A strong interaction between the imide ends of the molecule with the underlying gold substrate is evidenced by a large chemical shift in the imide carbon peaks, which is observed only for the first adsorbed layer.
Graphene Formation by Decomposition of C60 Perdigão, Luís M.A; Sabki, Syarifah N; Garfitt, Jason M ...
Journal of physical chemistry. C,
04/2011, Volume:
115, Issue:
15
Journal Article
Peer reviewed
Graphene is formed through the thermally induced decomposition of C60 in combination with a Ni thin film. After transfer to a SiO2 substrate, optical microscopy and Raman spectroscopy confirm the ...presence of graphene for films of C60 buried under nickel or alternatively for C60 adsorbed on a nickel surface. For buried films the graphene thickness is shown to depend on the fullerene dosage, with evidence of domain growth from nucleation sites separated by tens of micrometers. The adsorption of C60 and the changes arising from annealing are also monitored using scanning tunneling microscopy. We relate our results to previous studies of the decomposition of C60 on nickel and other transition metals.
Antimicrobial resistance (AMR) represents one of the most crucial threats to public health and modern health care. Previous studies have identified challenges with estimating the magnitude of the ...problem and its downstream effect on human health and mortality. To our knowledge, this study presents the most comprehensive set of regional and country-level estimates of AMR burden in the WHO European region to date.
We estimated deaths and disability-adjusted life-years attributable to and associated with AMR for 23 bacterial pathogens and 88 pathogen–drug combinations for the WHO European region and its countries in 2019. Our methodological approach consisted of five broad components: the number of deaths in which infection had a role, the proportion of infectious deaths attributable to a given infectious syndrome, the proportion of infectious syndrome deaths attributable to a given pathogen, the percentage of a given pathogen resistant to an antimicrobial drug of interest, and the excess risk of mortality (or duration of an infection) associated with this resistance. These components were then used to estimate the disease burden by using two counterfactual scenarios: deaths attributable to AMR (considering an alternative scenario where infections with resistant pathogens are replaced with susceptible ones) and deaths associated with AMR (considering an alternative scenario where drug-resistant infections would not occur at all). Data were solicited from a wide array of international stakeholders; these included research hospitals, surveillance networks, and infection databases maintained by private laboratories and medical technology companies. We generated 95% uncertainty intervals (UIs) for final estimates as the 25th and 975th ordered values across 1000 posterior draws, and models were cross-validated for out-of-sample predictive validity.
We estimated 541 000 deaths (95% UI 370 000–763 000) associated with bacterial AMR and 133 000 deaths (90 100–188 000) attributable to bacterial AMR in the whole WHO European region in 2019. The largest fatal burden of AMR in the region came from bloodstream infections, with 195 000 deaths (104 000–333 000) associated with resistance, followed by intra-abdominal infections (127 000 deaths 81 900–185 000) and respiratory infections (120 000 deaths 94 500–154 000). Seven leading pathogens were responsible for about 457 000 deaths associated with resistance in 53 countries of this region; these pathogens were, in descending order of mortality, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecium, Streptococcus pneumoniae, and Acinetobacter baumannii. Methicillin-resistant S aureus was shown to be the leading pathogen–drug combination in 27 countries for deaths attributable to AMR, while aminopenicillin-resistant E coli predominated in 47 countries for deaths associated with AMR.
The high levels of resistance for several important bacterial pathogens and pathogen–drug combinations, together with the high mortality rates associated with these pathogens, show that AMR is a serious threat to public health in the WHO European region. Our regional and cross-country analyses open the door for strategies that can be tailored to leading pathogen–drug combinations and the available resources in a specific location. These results underscore that the most effective way to tackle AMR in this region will require targeted efforts and investments in conjunction with continuous outcome-based research endeavours.
Bill & Melinda Gates Foundation, Wellcome Trust, and Department of Health and Social Care using UK aid funding managed by the Fleming Fund.
Abstract
Our objective was to evaluate the effects of mineral source supplementation on performance of finishing cattle when fed a high-concentrate diet for 89 days. Angus-Nellore bulls (iBW, n = 32; ...472 ± 4.93 kg; age, 18 months) were randomly assigned to two treatments in a completely randomized design, with sixteen replicates per treatment (animal as experimental unit). The control group received a diet containing inorganic minerals (i.e. sulphates) and the other group were supplemented with carbo-amino-phospho-chelates (TM, Tortuga Minerals) as a source of Cr, Se, Zn, Mn, S, Cu and Co. The DM intake and weight of the animals variables were assessed individually using the respective digital devices, Intergado electronic feeders and the Bosch Precision Livestock platform placed in the feedlot pen. Data were analyzed using the MIXED procedure of SAS and means were separated using PDIFF statement, adopting P< 0.05, and the initial BW as a covariate when significant. Supplementation with TM increased the ADG (1.74 vs. 1.55 kg/d, P< 0.01) and final BW (626.96 vs. 609.91kg, P< 0.01) compared with animals supplemented with inorganic sources. Likewise, animals supplemented with TM had better G:F (0.133 vs. 0.123 kg/kg, P< 0.05) and biological efficiency (146.41 vs 134,34 kg DMI/15 kg de carcass; P< 0.05), with no effect on DM intake (~12.94 kg, P=0.43). In addition, supplementation with TM increased final HCW in 13.44 kg (366.57 vs. 353.13 kg; P< 0.01) and carcass ADG in 0.15 kg/d (1.47 vs. 1.32 kg/d; P< 0.01). We conclude that supplementation with carbo-amino-phospho-chelates can be used as a tool to increase productive performance and efficiency by feedlot cattle.
Abstract
Our objective was to evaluate the effects of feed additives on ruminal pH of finishing cattle fed a 90%-concentrate diet. Twenty four 18-mo Angus-Nellore crossbred bulls (IBW, 456 ± 6,5 kg) ...were allocated in a completely randomized design to three treatments with eight replicates each, as follows: 1) Control (MON) - Sodium Monensin (26 mg/kg DM, Rumensin, Elanco Animal Health); 2) Crina® RumistarTM (CR) - a blend of essential oils, 90 mg/kg DM + exogenous α-amylase, 560 mg/kg DM) and 3) CR + HyD® (25-hydroxy-vitamin-D3 at 1 mg/animal/d, CRD, DSM Nutritional Products). The ruminal pH and temperature was monitored individually for 98 days, using a wireless bolus, (SmaXtec Animal Care, Austria). Data were analyzed using the Mixed procedure of SAS and means comparison evaluated by Tukey test at P< 0.05. During adaptation period (i.e. first 14 days), bulls fed CR and CRD had increased rumen mean pH (6.40 and 6.36 vs. 6.16; P < 0.01) and minimum pH (5.89 and 5.87 vs. 5.57; P < 0.01) than bulls fed MON. In addition, rumen pH from bulls fed CR spent less time below 6.0 than bulls fed MON (256.07 vs. 452.62 min/d; P = 0.05). Regarding the total period, bulls fed MON had lower mean (6.22 vs. 6.51 and 6.42, P < 0.01) and minimum rumen pH (5.60 vs. 5.92 and 5.85, P < 0.01) than bulls fed CR and CRD. Additionally, feeding MON increased time duration of rumen pH (390.79 min/day, P < 0.01) and had a larger area below 6.0 (81.52 min x pH units/day; P < 0.01). Moreover, the addition of monensin increased pH time duration below 5.8 (161.10 vs. 121.13 and 122.56 min/day; P = 0.02) compared with CR and CRD, and increased ruminal temperature (39.60 vs. 39.51 and 39.5 °C; P < 0.01). We conclude that feeding Crina® RumistarTM and Crina® RumistarTM HyD® increased the rumen pH of bulls.
Abstract
Our objective was to evaluate the effects of 3-Nitrooxypropanol (3-NOP) associated with different feed additives on performance, carcass characteristics and enteric methane (CH4) emissions. ...Twenty-seven Nellore bulls (initial BW, 410 ± 6 kg) were randomly allocated to three treatments with nine replicates per treatment (animal as experimental unit). The animals were fed a high-concentrate feedlot diet (R:C, 10:90) for 102 days, according with the following three treatments: 1) Control (CTRL): Sodium Monensin (26 mg/kg DM); 2) BOV: Control + 3-NOP (Bovaer;100 mg/kg DM); 3) BOVPERF (Bovaer Performance pack): Control + 3-NOP (100 mg/kg DM) + Essential oils (Crina Ruminants, 100 mg/kg DM) + 25-OH-Vitamin D3 (Hy-D, 0.10 mg/kg DM) + carbo-amino-phospho-chelate of chromium and zinc (0.4 and 60 mg/kg of DM, respectively). The gases emissions (CH4 and H2) and DMI variables were assessed individually, by using the GreenFeed and Intergado electronic system placed in the feedlot pen, respectively. The BW of Yearling bulls were recorded at the beginning and final of the experiment after 16-h fasting (Table). Data were analyzed by ANOVA using the PROC MIXED of SAS 9.4, in which means comparison were evaluated by Tukey test at P < 0.05. Feeding BOVPERF increased DM intake during adaptation period (P = 0.04), and over the total period (P < 0.01) compared with control with no difference on feed efficiency (P =0.61). There was no difference (P > 0.05) on DMI between CTRL and BOV. Animals fed BOVPERF had greater HCW (+13 kg, P = 0.04) and greater carcass ADG (+120 g/d, P = 0.04) compared with CTRL. Animals fed BOV and BOVPERF emitted 39% less CH4 (P < 0.01) and produced 292% more H2 (P < 0.01) compared with CTRL. In addition, there were significant reductions in methane intensity, as measured by the ratios CH4/kg DM (-42%; P < 0.01), and CH4/kg carcass ADG (-41.13%; P < 0.01) for animals fed with BOV and BOVPERF compared with CTRL. We conclude that feeding Bovaer performance pack reduces methane emissions and increases carcass production in feedlot beef cattle.
