The aim of this study was to evaluate
the antibacterial effect of ozone on suspension of three different bacteria inoculated in prepared canals of extracted human teeth.
Ozone was produced by special ...KP syringe of high frequency ozone generator Ozonytron (Biozonix, München, Germany) from aspirated atmospheric air by dielectric barrier discharge and applied through the tip of the syringe to the prepared root canal. The microorganisms used were
,
and
.
However, none of the methods was 100% effective against the three bacterial types in suspension. Application of ozone significantly decreased the absolute count of microorganisms (89.3%), as well as the count of each type of bacteria separately (
94.0%;
88.6% and
79.7%). Ozone generated by KP syringe was statistically more effective compared to NaOCl as positive control, for
and
.
The absolute count of
was statistically decreased without a statistically significant difference between the tested group and positive control, respectively. Among the three types of bacteria in suspension, KP probe had the lowest antimicrobial effect against
.
Aim: The aim of this study was to evaluate in vitro the antibacterial effect of ozone on suspension of three different bacteria inoculated in prepared canals of extracted human teeth. Material and ...methods: Ozone was produced by special KP syringe of high frequency ozone generator Ozonytron (Biozonix, Munchen, Germany) from aspirated atmospheric air by dielectric barrier discharge and applied through the tip of the syringe to the prepared root canal. The microorganisms used were Enterococcus faecalis, Staphylococcus aureus and Staphylococcus epidermidis. Results: However, none of the methods was 100% effective against the three bacterial types in suspension. Application of ozone significantly decreased the absolute count of microorganisms (89.3%), as well as the count of each type of bacteria separately (Staphylococcus aureus 94.0 %; Staphylococcus epidermidis 88.6% and Enterococcus faecalis 79.7%). Ozone generated by KP syringe was statistically more effective compared to NaOCl as positive control, for Staphylococcus aureus and Staphylococcus epidermidis. Conclusion: The absolute count of Enterococcus faecalis was statistically decreased without a statistically significant difference between the tested group and positive control, respectively. Among the three types of bacteria in suspension, KP probe had the lowest antimicrobial effect against Enterococcus faecalis. Key words Dental Pulp Cavity; Root Canal Therapy; Anti-Bacterial Agents; Ozone; Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis Svrha: Svrha ovog istrazivanja bila je procijeniti in vitro ucinkovitost ozona na suspenziju triju razlicitih vrsta bakterija inokluiranih u obradene korijenske kanale ekstrahiranih ljudskih zubi. Materijal i metode: Ozon je proizveden od aspiriranog zraka uporabom specijalne KP brizgalice visokofre-kvencijskoga generatora ozona Ozonytron (Biozonix, MUnchen, Njemacka) S pomocu dielektricnog barijernog izboja, te nastavka na strcaljki postavljenog u pripremljeni korijenski kanal. Ispitivani su mikroorganizmi Enterococcus faecalis, Staphylococcus aureus i Staphyloccocus epidermidis. Rezultati: Ni jedna metoda nije pokazala 100-postotnu ucinkovitost u eliminaciji navedenih bakterija u suspenziji. Primjena ozona znacajno je smanjila ukupan broj mikroorganizama (89,3 %), te broj svake vrste bakterija posebno (Staphyloccocus aureus--94,0 %; Staphyloccocus epidermidis--88,6% i Enterococcus faecalis--79,7 %). Ozon proizveden KP brizgalicom bio je statisticki znatno ucinkovitiji u eliminaciji Staphyloccocus aureus i Staphyloccocus epidermidis u usporedbi S NaOCl-om kao pozitivnom kontrolom. Zakljucak: Ukupan broj Enterococcus faecalis bio je statisticki manji, no bez znacajne statisticke razlike izmedu ispitivane i pozitivne kontrolne skupine. Izmedu triju vrsta bakterija u suspenziji, primjena KP strcaljke pokazala je najmanju ucinkovitost u eliminaciji Enterococcus faecalis. Kljucne rijeci zubna pulpa, komora; korijenski kanal, lijecenje; antibakterijska sredstva; ozon; Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis
Provider: - Institution: University of Zagreb. School of Dental Medicine. Department of Endodontics and Restorative Dentistry. - Data provided by Europeana Collections- Svrha je istraživanja bila ...odrediti fizičke i kemijske karakteristike terapijskog polja
visokofrekvencijskog generatora ozona.
U terapijskom polju je izmjeren spektar plazma elektrode u području 540-940 nm (atomske
linije Ne I i O I) dok se kod izboja kroz zrak bilježi i spektar mikro-izboja u području
290-440 nm (molekularne linije N2 i N2+). Na uzorku tkiva su izmjerene vrijednosti
efektivnog napona (22-95 mV), i efektivne struje (0,22-0,95 mA) na osnovu kojih su
procijenjene vrijednosti gustoće struje (4-19 μA/mm2) kao i snage (5-65 μW) te predane
energije u ovisnosti o vremenu izlaganja (0,14-10,75 mJ kod trajanja zahvata 30-
120 sekundi). Kod oba modaliteta primjene, u frekvencijskom području 33 kHz, zabilježena je
jakost magnetskog polja (1-3,07 A/m) i gustoće magnetskog toka (1,25-3,86 μT). Prilikom
pojave mikro-izboja u zračnom međuprostoru, u frekvencijskom području iznad 40 MHz, se
bilježi magnetsko polje jakosti (5-49 mA/m) te gustoće magnetskog toka od (6,5- 62,7 nT).
Jakost električnog polja na udaljenosti od 10 mm od elektrode iznosi 248-586 V/m. Tijekom
provedbe zahvata zabilježene su značajne promjene u temperaturi površine tkiva te one
ovisno o intenzitetu i trajanju zahvata (30-120 sek) iznose od 2.6 do 12,2°C. Statistička
analiza rezultata ukazala je na značajnu razliku u vrijednostima fizičkih veličinama s obzirom
na dva modaliteta primjene.
Na osnovu navedenih dokaza se zaključuje da djelovanje ispitivanog uređaja ne bi trebalo
pripisivati isključivo ozonu, kao što je to dosad bio slučaj, već kumulativnom učinku svih,
ovim istraživanjem dokazanih, fizičkih i kemijskih čimbenika koji su tijekom zahvata prisutni
u terapijskom polju.- A high-frequency ozone generator Ozonix (Biozonix GmbH, Germany) is classified as a
medical ozone generator, thus its therapeutic effects have, so far, been attributed exclusively
to ozone effect. The operative principle of the device and ozone production through a
dielectric barrier discharge using glass electrodes filled with a noble gas requires presence of
additional physical and chemical factors, besides ozone, in the treatment field during the
procedure. The purpose of the study was to determine the physical and chemical properties in
the treatment field of the said device. For that purpose, measuring of emission spectra, electric
and magnetic values as well as changes in surface temperature on tissue samples, as functions
of the selected intensity and exposure time, was conducted, and two basic application
modalities (contact discharge and air gap discharge) were used.
Materials and Methods: Optical emission spectroscopy was used to record the spectrum of a
plasma electrode’s glow discharge, a micro discharge occurring in the aerial interspace (air
gap) between the electrode and the targeted surface and the spectrum of the discharge through
a liquid medium on the surface of the tissue sample. By measuring electromagnetic values
present in the treatment field, physical values of electric and magnetic fields were recorded, as
well as currents passing through the treatment field. Thermographic measurements were used
to record temperature changes at different intensities (10-100%) and exposure durations (30,
60, 90 and 120 seconds).
Results: In the treatment field, two main emission spectra were recorded. Spectral lines of
plasma electrode’s glow discharge were recorded in the optical range between 540 and
940 nm, which were identified as atomic lines of neon (Ne I) and oxygen atoms (O I). This
spectrum is observed in all treatment modalities. During the formation of micro-discharges in
the air gap between the electrode and the treated surface, spectral lines were recorded in the
optical range between 290 and 440 nm, which were identified as molecular lines of N2 and N2+.
On the tissue samples (pork skin), as a function of output intensity (10-100%), electrical
values were recorded by use of oscilloscope and low impedance (Uin = 100) probe: RMS
voltage (22-95 mV), RMS current (0,22- 0.95 mA). These values were used to calculate
current density (4-19 μA/mm2) as well as power (5-65 μW). Using the same duration periods
as the ones used in the examination of temperature change at the tissue surface (30-120 sec),
the values of committed (delivered) energy were estimated (0,14-10,75 mJ).
Magnetic fields were recorded at two frequency ranges. In the frequency range of 33 kHz,
magnetic field was recorded in both treatment modalities as a function of intensity (10-100%)
for direct contact discharge H(1,93 ± 0,59 A/m), B (2,43 ± 0,75 μT), f (32151 ± 985Hz) and
air gap discharge H (2,5 ± 0,52 A/m), B (3,14 ± 0,65 μT), f (34526 ± 833 Hz). Only in the
case of micro-discharge formation in the air gap were additional peaks of magnetic field
observed in a wide frequency range from 40 to above 400 MHz. Due to the low intensity,
magnetic field values were calculated only for 41.5 MHz peak, where H field was
0,032 ± 0,014 A/m and B field was 40 ± 17,4 nT. At 10 mm distance from plasma electrode
surface, electric field strength was recorded in the range from 248 to 586 V/m depending on
the selected intensity (10-100%). During the treatment, a mild increase of temperature on the
surface of the tissue samples was recorded (2,6-12,2°C) as a function of intensity (20-100%)
and exposure time (30-120 seconds).
Conclusion: The results of the conducted research confirm the presence of mildly ionized
cold atmospheric plasma in the treatment field during air gap discharge. In both treatment
modalities, the presence of light in visible red and near infrared spectra, electric field,
magnetic fields, the currents flowing through the tissue and mild heat generated on the surface
of the tissue sample were confirmed in the treatment field. Furthermore, a significant
difference was determined in the values of physical properties with respect to the application
modality, where the values of temperature increase and magnetic field strengths are
significantly higher in the air gap modality whilst the values of electric current (and other
derived values) are lower compared to direct contact modality.
According to the results of this research, it can be concluded that treatment effects with the
tested devices must be attributed not only to ozone, but to cumulative effects of all physical
and chemical factors, the presence of which was recorded in the treatment field during this
research.- All metadata published by Europeana are available free of restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana
The cold plasma generator is a complex system in which many interrelated physical processes occur. The therapeutic device producing cold plasma is based on high-voltage high-frequency Tesla coil ...coupled with gas-filled glass plasma electrodes. The device is used for therapeutic purpose and thus highest level of safety is required. Simulations are a fast and efficient way to verify the measurements and to provide deeper insight into the problem. The aim of this study was to perform simulation and analysis of such system by finite element method using COMSOL Multiphysics. Key values of high importance were obtained and evaluated. 2-D axisymmetric and 3-D models of Tesla coil were made. Time dependent study was performed, values of magnetic and electric field were observed. Stationary analysis was performed for 3-D model, inductance and resistance of the coil were calculated.
Svrha: Svrha ovog istraživanja bila je procijeniti in vitro učinkovitost ozona na suspenziju triju različitih vrsta bakterija inokluiranih u obrađene korijenske kanale ekstrahiranih ljudskih zubi. ...Materijal i metode: Ozon je proizveden od aspiriranog zraka uporabom specijalne KP brizgalice visokofrekvencijskoga generatora ozona Ozonytron (Biozonix, München, Njemačka) s pomoću dielektričnog barijernog izboja, te nastavka na štrcaljki postavljenog u pripremljeni korijenski kanal. Ispitivani su mikroorganizmi Enterococcus faecalis, Staphylococcus aureus i Staphyloccocus epidermidis. Rezultati: Ni jedna metoda nije pokazala 100-postotnu učinkovitost u eliminaciji navedenih bakterija u suspenziji. Primjena ozona značajno je smanjila ukupan broj mikroorganizama (89,3 %), te broj svake vrste bakterija posebno (Staphyloccocus aureus – 94,0 %; Staphyloccocus epidermidis – 88,6 % i Enterococcus faecalis – 79,7 %). Ozon proizveden KP brizgalicom bio je statistički znatno učinkovitiji u eliminaciji Staphyloccocus aureus i Staphyloccocus epidermidis u usporedbi s NaOCl-om kao pozitivnom kontrolom. Zaključak: Ukupan broj Enterococcus faecalis bio je statistički manji, no bez značajne statističke razlike između ispitivane i pozitivne kontrolne skupine. Između triju vrsta bakterija u suspenziji, primjena KP štrcaljke pokazala je najmanju učinkovitost u eliminaciji Enterococcus faecalis.
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