Nanotechnology refers to the fabrication, characterization, and application of substances in nanometer scale dimensions for various ends. The influence of nanotechnology on the healthcare industry is ...substantial, particularly in the areas of disease diagnosis and treatment. Recent investigations in nanotechnology for drug delivery and tissue engineering have delivered high-impact contributions in translational research, with associated pharmaceutical products and applications. Over the past decade, the synthesis of nanofibers or nanoparticles via electrostatic spinning or spraying, respectively, has emerged as an important nanostructuring methodology. This is due to both the versatility of the electrospinning/electrospraying process and the ensuing control of nanofiber/nanoparticle surface parameters. Electrosprayed nanoparticles and electrospun nanofibers are both employed as natural or synthetic carriers for the delivery of entrapped drugs, growth factors, health supplements, vitamins, and so on. The role of nanofiber/nanoparticle carriers is substantiated by the programmed, tailored, or targeted release of their contents in the guise of tissue engineering scaffolds or medical devices for drug delivery. This review focuses on the nanoformulation of natural materials via the electrospraying or electrospinning of nanoparticles or nanofibers for tissue engineering or drug delivery/pharmaceutical purposes. Here, we classify the natural materials with respect to their animal/plant origin and macrocyclic, small molecule or herbal active constituents, and further categorize the materials according to their proteinaceous or saccharide nature.
Obesity is a global phenomenon and is associated with various types of cancer, including colon cancer. There is a growing interest for safe and effective bioactive compounds that suppress the risk ...for obesity-promoted colon cancer. Resveratrol (trans-3, 4', 5,-trihydroxystilbene), a stilbenoid found in the skin of red grapes and peanuts suppresses many types of cancers by regulating cell proliferation and apoptosis through a variety of mechanisms, however, resveratrol effects on obesity-promoted colon cancer are not clearly established.
We investigated the anti-proliferative effects of resveratrol on HT-29 and SW480 human colon cancer cells in the presence and absence of insulin like growth factor-1 (IGF-1; elevated during obesity) and elucidated the mechanisms of action using IGF-1R siRNA in HT-29 cells which represents advanced colon carcinogenesis.
Resveratrol (100-150 microM) exhibited anti-proliferative properties in HT-29 cells even after IGF-1 exposure by arresting G0/G1-S phase cell cycle progression through p27 stimulation and cyclin D1 suppression. Treatment with resveratrol suppressed IGF-1R protein levels and concurrently attenuated the downstream Akt/Wnt signaling pathways that play a critical role in cell proliferation. Targeted suppression of IGF-1R using IGF-1R siRNA also affected these signaling pathways in a similar manner. Resveratrol treatment induced apoptosis by activating tumor suppressor p53 protein, whereas IGF-1R siRNA treatment did not affect apoptosis. Our data suggests that resveratrol not only suppresses cell proliferation by inhibiting IGF-1R and its downstream signaling pathways similar to that of IGF-1R siRNA but also enhances apoptosis via activation of the p53 pathway.
For the first time, we report that resveratrol suppresses colon cancer cell proliferation and elevates apoptosis even in the presence of IGF-1 via suppression of IGF-1R/Akt/Wnt signaling pathways and activation of p53, suggesting its potential role as a chemotherapeutic agent.
Electrospun nanofibrous membranes (ENMs) are used in a variety of applications, including sensors, tissue engineering, air filtration, energy, and reinforcement in composite materials. Recently, they ...have gained an interest in the field of liquid filtration. The membranes, surface, bulk, and overall architecture play an important role in the filtration properties and hence the right characterization technique needs to be established, which will pave the way for future developments in the field of filtration. In this article, we have reviewed the recent advances in ENMs for liquid separation application.
Heart disease is the leading cause of mortality in many industrialized nations and is often related to irregularities in electrical function that can radically damage cardiac functioning. The aim of ...this study is to develop a novel therapeutic hybrid scaffold that can couple electrical, mechanical, and biological properties, desirable for cardiac tissue regeneration. BSA/PVA scaffolds are fabricated in the ratio 2:1 and gold nanoparticles (AuNPs) embedded scaffolds in the ratios BSA/PVA/Au of 2:1:0.1 (lower concentration) and BSA/PVA/Au of 2:1:0.4 (higher concentration) by electrospinning. The scaffolds are characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), contact angle, Fourier transform infrared (FTIR) spectroscopy, and tensile testing to analyze the fiber morphology, AuNP distribution, hydrophilicity, surface functional groups, and mechanical properties of the scaffolds, respectively. Results show that ex vivo pretreatment of MSCs using 5‐aza and AuNPs loaded conductive nanofibrous construct could lead to enhanced cardiomyogenic differentiation and result in superior biological and functional effects on infarcted myocardium regeneration.
A novel therapeutic Au nanoparticles loaded PVA/BSA nanofibers is discussed in the present study for infarcted myocardium regeneration. These scaffolds can be used to improve the functional activity of differentiated MSCs by increasing the gap junction protein, connexin 43 (Cx43). This approach opens the frontiers for creating functional cardiac patch with desirable properties for enhanced cardiac regeneration.
3,4,5-Trifluorobenzeneboronic acid catalysed, ionic liquid mediated facile synthesis of 4-pyrazolyl 1,4-dihydropyridines at room temperature by the cyclocondensation of ethyl 3-aminocrotonate, ...pyrazole aldehyde and a β-keto ester is reported. The procedure adopted was found to be eco-benign, facile at room temperature and better than the conventional, bmimCl mediated and InCl
3 catalysed, bmimCl mediated 1,4-dihydropyridine syntheses.
Graphical Abstract
Abstract
Disinformation campaigns are prevalent, affecting vaccination coverage, creating uncertainty in election results, and causing supply chain disruptions, among others. Unfortunately, the ...problems of misinformation and disinformation are exacerbated due to the wide availability of online platforms and social networks. Naturally, these emerging disinformation networks could lead users to engage with critical infrastructure systems in harmful ways, leading to broader adverse impacts. One such example involves the spread of false pricing information, which causes drastic and sudden changes in user commodity consumption behavior, leading to shortages. Given this, it is critical to address the following related questions: (i) How can we monitor the evolution of disinformation dissemination and its projected impacts on commodity consumption? (ii) What effects do the mitigation efforts of human intermediaries have on the performance of the infrastructure network subject to disinformation campaigns? (iii) How can we manage infrastructure network operations and counter disinformation in concert to avoid shortages and satisfy user demands? To answer these questions, we develop a hybrid approach that integrates an epidemiological model of disinformation spread (based on a susceptible-infectious-recovered model, or SIR) with an efficient mixed-integer programming optimization model for infrastructure network performance. The goal of the optimization model is to determine the best protection and response actions against disinformation to minimize the general shortage of commodities at different nodes over time. The proposed model is illustrated with a case study involving a subset of the western US interconnection grid located in Los Angeles County in California.
Bivalent proximity-inducing compounds represent a novel class of small molecule therapeutics with exciting potential and new challenges. The most prominent examples of such compounds are utilized in ...targeted protein degradation where E3 ligases are hijacked to recruit a substrate protein to the proteasome via ubiquitination. In this review we provide an overview of the current state of E3 ligases used in targeted protein degradation, their respective ligands as well as challenges and opportunities that present themselves with these compounds.
Mimicking porous topography of natural extracellular matrix is advantageous for successful regeneration of damaged tissues or organs. Nanotechnology being one of the most promising and growing ...technology today shows an extremely huge potential in the field of tissue engineering. Nanofibrous structures that mimic the native extracellular matrix and promote the adhesion of various cells are being developed as tissue‐engineered scaffolds for skin, bone, vasculature, heart, cornea, nervous system, and other tissues. A range of novel biocomposite materials has been developed to enhance the bioactive or therapeutic properties of these nanofibrous scaffolds via surface modifications, including the immobilization of functional cell‐adhesive ligands and bioactive molecules such as drugs, enzymes, and cytokines. In skin tissue engineering, usage of allogeneic skin is avoided to reestablish physiological continuity and also to address the challenge of curing acute and chronic wounds, which remains as the area of exploration with various biomimetic approaches. Two‐dimensional, three‐dimensional scaffolds and stem cells are presently used as dermal regeneration templates for the treatment of full‐thickness skin defects resulting from injuries and severe burns. The present review elaborates specifically on the fabrication of nanofibrous structured strategies for wound dressings, wound healing, and controlled release of growth factors for skin tissue regeneration.
System level diagnosis, an area pioneered by Preparata, Metze and Chien, has been a dominant area of research in the broader area of fault-tolerant computing since the proposition of the PMC model. ...In this paper, we study the fault diagnosis problem for systems in hybrid fault circumstances where both node and link faults may occur. Under our diagnosis model, a test involves two adjacent processors and the link between them. We propose the definition of consistent faulty pairs and distinguishable faulty pairs. Given an assignment of testing links, we establish necessary and sufficient conditions for distinguishable faulty pairs. Finally, we introduce parameters to measure the fault diagnosis capability of multiprocessor systems under this model and as an application the fault diagnosis capability of hypercubes under this model has been evaluated.
Conventional small-molecule drugs (SMDs) are compounds characterized by low molecular weight, high cell permeability, and high selectivity. In clinical translation, SMDs are regarded as good ...candidates for oral drug formulation. SMD inhibitors play an important role in cancer treatment; however, resistance and low effectiveness have been major bottlenecks in clinical application. Generally, only 20% of cell proteins can potentially be targeted and have been developed as SMDs; thus, some types of tumor targets are considered “undruggable.” Among these are transcription factors (TFs), an important class of proteins that regulate the occurrence, formation, and development of tumors. It is difficult for SMDs and macromolecular drugs to identify bioactive sites in TFs and hence for use as pharmacological inhibitors in targeting TF proteins. For this reason, technologies that enable targeted protein degradation, such as proteolysis-targeting chimera or molecular glues, could serve as a potential tool to solve these conundrums.
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