As the mechanical dual of a capacitor via the force-current analogy, an inerter has been successfully applied in various fields such as automotive engineering, civil engineering and aerospace ...engineering. The introduction of an inerter not only allows the use of network synthesis analogy to design mechanical layouts but also opens the door to adopt electrical element impedances to simulate the corresponding mechanical elements. This paper combines both of these ideas and presents a new form of a mechatronic inerter, namely the hydraulic electric inerter (HEI), which consists of a hydraulic piston inerter and a linear motor. On the basis of the HEI device, a bicubic impedance function is considered in the optimal design of a vehicle suspension system employing both mechanical elements and electrical elements. In addition, a methodology for reducing the order of the bicubic impedance function is proposed, and the network is finally realized by utilizing inerter, spring and damper elements. Then, by comparison with a passive suspension, the advantages of the new vehicle ISD (inerter-spring-damper) suspension, called the vehicle HE-ISD (hydraulic electric-inerter spring damper) suspension, are demonstrated by numerical simulations. Finally, a HEI device is designed, and bench tests of the vehicle HE-ISD suspension are carried out. Experimental results indicate that, the vehicle HE-ISD suspension is superior to the passive suspension system, the RMS (root-mean-square) value of the suspension working space is improved by 19.97%, and the RMS value of the dynamic tire load is improved by 10.21%.
LncRNA is a kind of non-coding RNA and its research is more popular in recent years, which has more than 200 nucleotides. It plays a significant part in various biological functions, including ...chromosome modification, genome modification, transcriptional activation, transcriptional interference, and other processes. FTX, at the center of the X chromosome inactivation and it has been shown that lncRNA FTX regulates cancer cells' development, migration, and invasion in many studies.
Relevant literature was collected through the PubMed system search and is summarized in this article.
LncRNA FTX abnormally increased in tumor cells, such as liver cancer, stomach cancer, leukemia, renal cell carcinoma, colorectal cancer, glioma, osteosarcoma, etc. However, the expression level decreased in temporal lobe epilepsy, liver cirrhosis, heart failure, etc. Conclusion: FTX may be an important regulatory factor and a potential therapeutic target in cancers.
The effect of gold nanoparticles on lung cancer cells is not yet clear. In this study, we investigated the cytotoxicity and cell invasion activity of lung cancer cells after treatment with gold ...nanoparticles and showed that small gold nanoparticles can be endocytosed by lung cancer cells and that they facilitate cell invasion. The growth of A549 cells was inhibited after treatment with 5-nm gold nanoparticles, but cell invasion increased. Endocytosed gold nanoparticles (size, 10 nm) notably promoted the invasion activity of 95D cells. All these effects of gold nanoparticles were not seen after treatment with larger particles (20 and 40 nm). The enhanced invasion activity may be associated with the increased expression of matrix metalloproteinase 9 and intercellular adhesion molecule-1. In this study, we obtained evidence for the effect of gold nanoparticles on lung cancer cell invasion activity in vitro. Moreover, matrix metalloproteinase 9 and intercellular adhesion molecule-1, key modulators of cell invasion, were found to be regulated by gold nanoparticles. These data also demonstrate that the responses of the A549 and 95D cells to gold nanoparticles have a remarkable relationship with their unique size-dependent physiochemical properties. Therefore, this study provides a new perspective for cell biology research in nanomedicine.
Inerter, a new type of mass element, can increase the inertia of motion between two endpoints. In order to study the dynamic inertia effect of inerter–spring–damper suspension for heavy vehicle on ...ride comfort and road friendliness, the inerter–spring–damper suspension is applied and its mechanism is studied. This paper establishes a half vehicle model of inerter–spring–damper suspension for heavy vehicle. The parameters of inerter–spring–damper suspension for heavy vehicle are optimized by multi-objective genetic algorithm and system simulations are carried out. The parametric influence of different spring stiffness, damping coefficient, inertance, and load on suspension performance is also studied. The simulation results demonstrate that the centroid acceleration and pitch angular acceleration are improved by 24.90% and 23.54%, respectively, and the comprehensive road damage coefficient is reduced by 4.05%. The results illustrate that the inerter–spring–damper suspension can decrease the vertical vibration of vehicle suspension especially in low frequency and reduce the road damage. The analyses of suspension parameters perturbation reveal their different effect laws of the different wheels on vehicle ride comfort and road friendliness, which provide a theoretical basis for setting parameters of inerter–spring–damper suspension.
Background As a human tumor disease, head and neck squamous cell carcinoma (HNSCC) is associated with a high mortality rate worldwide. Nicotinic acetylcholine receptors (nAChRs) are transmembrane ...receptor proteins and exert their biological effects following activation by nicotine. We aimed to construct a prognostic signature based on the expression of nAChRs among smokers with HNSCC. Methods The transcriptome profile of nAChRs was obtained from The Cancer Genome Atlas (TCGA). Following the integration of survival information, univariate Cox regression and least absolute shrinkage and selection operator (LASSO) analyses were performed to screen the prognosis-related nAChRs and construct a prognostic signature. Kaplan-Meier (KM), receiver operating characteristic (ROC), principal component analysis (PCA), and independent prognostic analysis were utilized to verify the predictive power of the nAChR-associated prognostic signature. The expression of alpha5 nAChR in clinical samples was verified by quantitative reverse transcriptase PCR. Results Subunits alpha2, alpha5, alpha9, and beta4 were related to the prognosis. The prognostic signature comprised the expression of subunits alpha5, alpha9, and beta4. The nAChR-associated signature showed high sensitivity and specificity for prognostic prediction and was an independent factor for overall survival. Based on the clinical variables and expression of nAChRs, a nomogram was constructed for predicting the outcomes of HNSCC patients who were smokers in the clinical settings. In clinical specimens, alpha5 nAChR showed high expression in HNSCC tissues, especially among smokers. Conclusions The nAChR-associated signature constructed in this study may provide a better system for the classification of HNSCC patients and facilitate personalized treatment according to their smoking habits. Keywords: Smoking, nAChRs, HNSCC, Prognosis, Outcome
Recently, hub motor driven vehicles (HMDV) have been recognized as the ideal configuration of electric vehicles in the future. However, the adverse effect of its special structure configuration ...seriously affects the vehicles dynamic performance, and becomes the bottleneck of its further development and industrial application. In this paper, the characteristics of inertial suspension adjusting vertical motion inertia are utilized to suppress the HMDV adverse effect. Firstly, the coupling model of vehicle suspension and hub motor is established, and the coupling excitation of road surface and unbalanced electromagnetic force on vehicle suspension is studied. Aiming at the influence mechanism of the adverse effect, an inertial suspension model of the skyhook and groundhook (SH-GH) algorithm is established. Moreover, with the primary goal of improving the ride comfort, an inertial suspension and the mixed skyhook and power-driven-damper (SH-PDD) algorithm are combined. Particle swarm optimization (PSO) is utilized to optimize suspension performance. The results show that the RMS value of body acceleration, unbalanced radial force and dynamic tire load of inertial suspension decreased by 10.91%, 50% and 8.53%. It is proved that its characteristics can effectively suppress the HMDV adverse effects. The SH-GH inertial suspension also further reduces the vehicle performance index. The SH-PDD inertial suspension reduces the RMS value of body acceleration by 27.45%, significantly improves the vehicle ride comfort. In general, the inertial suspension proposed in this paper can effectively suppress the adverse effect of HMDV vertical vibration, and improve the HMDV ride comfort and road friendliness. It provides a new direction for the research of suppressing the adverse effect of HMDV vertical vibration.
The mechanism and gene markers of head and neck squamous cell carcinoma (HNSCC), a common malignant tumor, have not yet been identified. The aim of this study was to identify the key genes and ...pathways associated with HNSCC and to further analyze its molecular mechanism and prognostic significance. In this study, the expression profile chip data GSE6631 from Gene Expression Omnibus (GEO) included paired HNSCC tumor and normal samples from 22 patients; the RNAseq tertiary dataset of HNSCC and corresponding clinical information from The Cancer Genome Atlas (TCGA) included biological information of 12 normal head and neck tissues and 111 HNSCC sample tissues. Differentially expressed genes (DEGs) were screened by R software, and the pathway enrichment analysis of DEGs was performed by DAVID, String, and Sytoscape software programs. Combining the GEO and the TCGA databases, we used bioinformatics technology to screen out 50 DEGs in HNSCC and enrich the biological functions and key pathways of HNSCC. Then we performed Gene Ontology (GO) enrichment analysis, the Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway analysis, protein-protein interaction (PPI) analysis, and survival analysis on these DEGs. Using CMap, we identified candidate small molecules that might reverse HNSCC gene expression. Finally, four most important small molecules that could provide more reliable biomarkers for early diagnosis and individualized control of HNSCC were identified.
Tight junction dysfunction plays a vital role in some chronic inflammatory diseases. Pro-inflammatory cytokines, especially tumor necrosis factor alpha (TNF-α), act as important factors in intestinal ...epithelial tight junction dysfunction during inflammatory conditions. Autophagy has also been shown to be crucial in tight junction function and claudin-2 expression, but whether autophagy has an effect on the change of claudin-2 expression and tight junction function induced by TNF-α is still unknown. To answer this question, we examined the expression of claudin-2 protein, transepithelial electrical resistance (TER), and permeability of cell monolayers, autophagy flux change, and lysosomal pH after TNF-α with or without PP242 treatment. Our study showed that claudin-2 expression, intestinal permeability, microtubule-associated protein 1 light chain 3B II (LC3B-II) and sequestosome 1 (P62) expression largely increased while TER values decreased in TNF-α treated cell monolayers. Further research using 3-methyladenine (3-MA), bafilomycin A1, and ad-mCherry-GFP-LC3B adenovirus demonstrated that LC3B-II increase induced by TNF-α was attributed to the inhibition of autophagic degradation. Moreover, both qualitative and quantitative method confirmed the increase of lysosomal pH, and mammalian target of rapamycin (mTOR) inhibitor PP242 treatment relieved this elevation. Moreover, PP242 treatment also alleviated the change of autophagy flux, TER, and claudin-2 expression induced by TNF-α. Therefore, we conclude that increase of claudin-2 levels and intestinal epithelial tight junction dysfunction are partly caused by the inhibition of autophagic degradation in TNF-α treated cell monolayers.
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