Fullerenation of polycarbonate (PC) is achieved by direct reaction of C60 with PC using AlCl3 as catalyst. Stirring a 1,1,2,2-tetrachloroethane solution of C60 and PC in the presence of AlCl3 under ...nitrogen at 140 °C for 24 h attached C60 to the PC chains, yielding fullerenated PCs (C60−PCs) with a C60 content up to 2.16 wt %. The C60−PCs are soluble in tetrahydrofuran (THF) and chloroform, and IR, UV, and NMR analyses suggest that they possess the molecular structure H x C60(PC) x with x < 1 on the average. The C60−PCs limit strong 532-nm optical pulses more effectively than the parent C60. Light transmission spectra of the C60−PC solutions red-shift with increasing concentration, with their cutoff wavelengths predictably tunable by simply changing their concentrations.
BACKGROUND：Pancreatic stellate cells（PSCs）play a critical role in the development of pancreatic fibrosis.In this study we used a novel method to isolate and culture rat PSCs and then investigated the ...inhibitory effects of adipose-derived stem cells（ADSCs）on activation and proliferation of PSCs.METHODS：Pancreatic tissue was obtained from SpragueDawley rats for PSCs isolation.Transwell cell cultures were adopted for co-culture of ADSCs and PSCs. PSCs prolifera- tion and apoptosis were determined using CCK-8 and flow cytometry, respectively, a-SMA expressions were analyzed using Western blotting. The levels of cytokines nerve growth factor （NGF）, interleukin-10 （IL-10） and transforming growth factor-ill （TGF-31） in conditioned medium were detected by ELISA. Gene expression （MMP-2, MMP-9 and TIMP-1） was analyzed using qRT-PCR. RESULTS： This method produced 17.6_＋6.5 ~ 103 ceils per gram of the body weight with a purity of 90%-95% and a viability of 92%-97%. Co-culture of PSCs with ADSCs significantly inhib- ited PSCs proliferation and induced PSCs apoptosis. Moreover, a-SMA expression was significantly reduced in PSCs＋ADSCs compared with that in PSC-only cultures, while expression of fibrinolytic proteins （e.g., MMP-2 and MMP-9） was upregulated and anti-fibrinolytic protein （TIMP-1） was downregulated. In addition, NGF expression was up-regulated, but IL-10 and TGF-β1 expressions were down-regulated in the coculture conditioned medium compared with those in the PSC- only culture medium. CONCLUSIONS： This study provided an easy and reliable technique to isolate PSCs. The data demonstrated the inhibitory effects of ADSCs on the activation and proliferation of PSCs in vitro.
