Anaerobic digestion is promising technology to recover energy from waste activated sludge. However, the sludge digestion is limited by its low efficiency of hydrolysis-acidification. Zero valent iron ...(ZVI) as a reducing material is expected to enhance anaerobic process including the hydrolysis-acidification process. Considering that, ZVI was added into an anaerobic sludge digestion system to accelerate the sludge digestion in this study. The results indicated that ZVI effectively enhanced the decomposition of protein and cellulose, the two main components of the sludge. Compared to the control test without ZVI, the degradation of protein increased 21.9% and the volatile fatty acids production increased 37.3% with adding ZVI. More acetate and less propionate are found during the hydrolysis-acidification with ZVI. The activities of several key enzymes in the hydrolysis and acidification increased 0.6-1 time. ZVI made the methane production raise 43.5% and sludge reduction ratio increase 12.2 percent points. Fluorescence in situ hybridization analysis showed that the abundances of hydrogen-consuming microorganisms including homoacetogens and hydrogenotrophic methanogens with ZVI were higher than the control, which reduced the H2 accumulation to create a beneficial condition for the sludge digestion in thermodynamics.
Background
The incidence of primary liver cancer (PLC) continues to increase worldwide. The incidence trends and patterns of PLC associated with different age at diagnosis remain unknown.
Methods
We ...collected detailed information on PLC between 1990 and 2017 from Global Burden of Disease Study 2017. Estimated annual percentage changes in the PLC age‐standardized incidence rate (ASR) diagnosed by age, sex, region, and etiology were calculated to quantify the temporal trends in PLC ASR.
Results
Globally, the number of PLC cases for which the age at diagnosis was <30 years decreased from 17,381 in 1990 to 14,661 in 2017, whereas the number of PLC cases diagnosed at age 30 to 59 and ≥60 years old increased from 216,561 and 241,189 in 1990 to 359,770 and 578,344 in 2017, respectively. The ASR of PLC cases with age at diagnosis <30 years and between 30 and 59 years decreased in both sexes, whereas the ASR of PLC with age at diagnosis ≥60 years increased in males and remained stable in females at the global level. Males had a more dramatic increase in PLC diagnosed at age ≥60 years but a milder decrease in PLC diagnosed between 30 and 59 years of age. This decrease was attributed largely to the reduction in PLC caused by hepatitis B and hepatitis C and was consistent in most regions except for developed countries, in which the ASR of PLC increased irrespective of sex and age. The ASR of PLC due to nonalcoholic steatohepatitis (NASH) increased by the greatest magnitude in most regions.
Conclusion
PLC in highly endemic regions has been partly alleviated due to the potent control of hepatitis, especially among young and middle‐aged people. However, an unfavorable trend was observed in most developed countries and in elderly populations. As such, PLC prevention schedules should give more attention to NASH and elderly patients.
There is a significant increase in incidence of late‐onset liver cancer. The most pronounced increase occurs in liver cancer due to nonalcoholic steatohepatitis.
Early detection has the potential to reduce cancer mortality, but an effective screening test must demonstrate asymptomatic cancer detection years before conventional diagnosis in a longitudinal ...study. In the Taizhou Longitudinal Study (TZL), 123,115 healthy subjects provided plasma samples for long-term storage and were then monitored for cancer occurrence. Here we report the preliminary results of PanSeer, a noninvasive blood test based on circulating tumor DNA methylation, on TZL plasma samples from 605 asymptomatic individuals, 191 of whom were later diagnosed with stomach, esophageal, colorectal, lung or liver cancer within four years of blood draw. We also assay plasma samples from an additional 223 cancer patients, plus 200 primary tumor and normal tissues. We show that PanSeer detects five common types of cancer in 88% (95% CI: 80-93%) of post-diagnosis patients with a specificity of 96% (95% CI: 93-98%), We also demonstrate that PanSeer detects cancer in 95% (95% CI: 89-98%) of asymptomatic individuals who were later diagnosed, though future longitudinal studies are required to confirm this result. These results demonstrate that cancer can be non-invasively detected up to four years before current standard of care.
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•Primary liver cancer incidence is still on the rise at the global level.•Pronounced increases in liver cancer incidence were mostly observed in countries with high socio-demographic ...indexes.•Liver cancer has been alleviated in some regions due to the control of HBV and HCV infections.•HCV-related liver cancer might be an important public health issue in the near future.
Liver cancer is a common malignant neoplasm worldwide. The etiologies for liver cancer are diverse and the incidence trends of liver cancer caused by specific etiologies are rarely studied. We therefore aimed to determine the pattern of liver cancer incidence, as well as temporal trends.
We collected detailed information on liver cancer etiology between 1990–2016, derived from the Global Burden of Disease study in 2016. Estimated annual percentage changes (EAPCs) in liver cancer age standardized incidence rate (ASR), by sex, region, and etiology, were calculated to quantify the temporal trends in liver cancer ASR.
Globally, incident cases of liver cancer increased 114.0% from 471,000 in 1990 to 1,007,800 in 2016. The overall ASR increased by an average 0.34% (95% CI 0.22%–0.45%) per year in this period. The ASR of liver cancer due to hepatitis B, hepatitis C, and other causes increased between 1990 and 2016. The corresponding EAPCs were 0.22 (95% CI 0.08–0.36), 0.57 (95% CI 0.48–0.66), and 0.51 (95% CI 0.41–0.62), respectively. The ASR of liver cancer due to reported alcohol use remained stable (EAPC = 0.10, 95% CI −0.06–0.25). This increasing pattern was heterogeneous across regions and countries. The most pronounced increases were generally observed in countries with a high socio-demographic index, including the Netherlands, the UK, and the USA.
Liver cancer remains a major public health concern globally, though control of hepatitis B and C virus infections has contributed to the decreasing incidence in some regions. We observed an unfavorable trend in countries with a high socio-demographic index, suggesting that current prevention strategies should be reoriented, and much more targeted and specific strategies should be established in some countries to forestall the increase in liver cancer.
Liver cancer is a common malignant neoplasm worldwide. The incidence patterns of liver cancer caused by different etiologies varied considerably across the world. In this study, we aim to determine the pattern of liver cancer incidence as well as the temporal trends, thereby facilitating the establishment of more tailored prevention strategies for liver cancer.
p53, circRNAs and miRNAs are important components of the regulatory network that activates the EMT program in cancer metastasis. In prostate cancer (PCa), however, it has not been investigated ...whether and how p53 regulates EMT by circRNAs and miRNAs. Here we show that a Amotl1-derived circRNA, termed circAMOTL1L, is downregulated in human PCa, and that decreased circAMOTL1L facilitates PCa cell migration and invasion through downregulating E-cadherin and upregulating vimentin, thus leading to EMT and PCa progression. Mechanistically, we demonstrate that circAMOTL1L serves as a sponge for binding miR-193a-5p in PCa cells, relieving miR-193a-5p repression of Pcdha gene cluster (a subset of the cadherin superfamily members). Accordingly, dysregulation of the circAMOTL1L-miR-193a-5p-Pcdha8 regulatory pathway mediated by circAMOTL1L downregulation contributes to PCa growth in vivo. Further, we show that RBM25 binds directly to circAMOTL1L and induces its biogenesis, whereas p53 regulates EMT via direct activation of RBM25 gene. These findings have linked p53/RBM25-mediated circAMOTL1L-miR-193a-5p-Pcdha regulatory axis to EMT in metastatic progression of PCa. Targeting this newly identified regulatory axis provides a potential therapeutic strategy for aggressive PCa.
Background
The incidence and mortality of esophageal cancer are high, with 5.90 new cases and 5.48 deaths per 100 000 people worldwide in 2017. The prognosis of esophageal cancer is poor, with an ...overall 5‐year survival rate of less than 20%. Esophageal cancer in different geographical locations has different etiologies, and the incidence and mortality of esophageal cancer continue to rise in some regions.
Methods
We collected incidence and mortality data by age and gender for 195 countries and territories from 1990 to 2017 in the Global Burden of Disease (GBD) database. And we used these data to calculate the estimated annual percentage change (EAPC) to quantify trends in morbidity and mortality. Then we analyzed the gender‐ and age‐specific incidence and mortality in esophageal cancer to targeted high‐risk populations. Finally, we analyzed the correlation between the age‐standardized mortality rate (ASMR) and both the EAPC and social‐demographic index (SDI), and we calculated the Pearson correlation coefficient.
Results
We found that Malawi, East Asia, and high‐middle SDI regions had the highest age‐standardized incidence rate (ASIR) and ASMR, and the ASIR and ASMR in western Sub‐Saharan Africa showed an upward trend. Our study also showed that the incidence and mortality in esophageal cancer were highest in men and in the 70+ years age group, and they presented a decreasing trend in most regions, but the 15‐49 years age groups in Australasia, Caribbean, and Oceania and the 70+ years age group in High‐Income North America, Oceania and high‐SDI regions presented an increasing trend. There were significant negative associations between ASMR at baseline and EAPC and between ASMR and SDI in 2017.
Conclusion
By analyzing the global distribution of incidence and mortality in esophageal cancer, trends over time, and gender and age specificity, we can understand the heterogeneity of its global trends. This heterogeneity can help us to identify high‐risk groupsand to provide clues for the exploration of the etiology and early prevention of the disease.
Our study explores areas where morbidity and mortality continue to rise, and refines them into populations of different ages and genders. The heterogeneity of the incidence and mortality trends in esophageal cancer means that we can target high‐risk populations and provide clues for the exploration of the etiology and early prevention of the disease.
Metabolic dysfunction-associated fatty liver disease (MAFLD) is a significant health issue closely associated with multiple metabolic dysfunctions. The association between MAFLD and cancer risk is ...yet unknown.
UK Biobank study participants were diagnosed for the presence of MAFLD at baseline. A multivariable Cox regression model was performed to examine the associations of MAFLD with incident events in 24 specific cancers.
We included 352,911 individuals (37.2% with MAFLD), among whom 23,345 developed cancers. Compared with non-MAFLD, MAFLD was significantly associated with 10 of the 24 examined cancers, including corpus uteri (hazard ratio HR = 2.36, 95% CI 1.99–2.80), gallbladder (2.20, 1.14–4.23), liver (1.81, 1.43–2.28), kidney (1.77, 1.49–2.11), thyroid (1.69, 1.20–2.38), esophagus (1.48, 1.25–1.76), pancreas (1.31, 1.10–1.56), bladder (1.26, 1.11–1.43), breast (1.19, 1.11–1.27), and colorectal and anus cancers (1.14, 1.06–1.23). The associations of MAFLD with liver, esophageal, pancreatic, colorectal and anal and bladder cancers and malignant melanoma were strengthened in males, and associations with kidney, thyroid, and lung cancers were increased in females. The associations of MAFLD with the risk of liver, kidney, and thyroid cancers remained significant after further adjusting for the waist circumference or body mass index and the number of metabolic syndrome components based on the main models. The risk-increasing allele of PNPLA3 rs738409 significantly amplified the association of MAFLD with the risk of liver and kidney cancers.
MAFLD is associated with an increased risk of a set of cancers, but the effect substantially varies by site. MAFLD deserves higher priority in the current scheme of cancer prevention.
•MAFLD is associated with both intrahepatic and extrahepatic cancers.•Associations of MAFLD with cancer risk varied by cancer site and sex.•Risk allele of PNPLA3 rs738409 significantly amplified the risk of liver and kidney cancers.•MAFLD warrants higher priority in the current scheme of cancer prevention.
Heavy drinking was well associated with an increased risk of hepatocellular carcinoma (HCC), whereas the effect of low-to-moderate drinking on HCC remains under debate.
Participants from the UK ...Biobank with detailed information on alcohol use and free of common diseases were included. Daily pure alcohol intake (g/day) was calculated, and the predominant alcoholic beverage type was assigned for each participant. Additive Cox regression model and nonlinear Mendelian randomization (NLMR) analyses were performed to evaluate the association of alcohol intake with HCC.
Of 329,164 participants (52.3% females, mean SD age = 56.7 8.0 years), 201 incident HCC cases were recorded during the median follow-up of 12.6 years. The best-fitted Cox regression model suggested a J-shaped relationship between daily alcohol intake level and HCC risk. However, NLMR analysis did not detect a nonlinear correlation between alcohol use and HCC (nonlinearity P-value: 0.386). The J-shaped correlation pattern was detected only in subjects who mainly drank wine but not in those who mainly drank beer, spirits, or fortified wine. Moderate wine drinking showed a significant alanine transaminase (ALT)- and aspartate aminotransferase-lowering effect compared to that of the nondrinkers. In low-risk populations of HCC including women, people aged < 60 years, subjects with normal ALT levels, and those carrying non-risk genotypes of PNPLA3 rs738409 and TM6SF2 rs58542926, we observed a J-shaped correlation between alcohol use and HCC; however, a positive dose-response correlation was found in their respective counterparts, even in those predominantly drinking wine.
Low-to-moderate drinking may be inversely associated with the risk of HCC in low-risk populations, which may be largely driven by wine drinking. However, those in high-risk populations of HCC, such as men and older people, and those with abnormal ALT levels and carry genetic risk variants, should abstain from drinking alcohol. Given the small HCC case number, further validations with larger case numbers are warranted in future works.
Background Mitochondrial (MT) dysfunction is a hallmark of liver diseases. However, the effects of functional variants such as protein truncating variants (PTVs) in MT-related genes on the risk of ...liver diseases have not been extensively explored. Methods We extracted 60,928 PTVs across 2466 MT-related nucleus genes using whole-exome sequencing data obtained from 442,603 participants in the UK Biobank. We examined their associations with liver dysfunction that represented by the liver-related biomarkers and the risks of chronic liver diseases and liver-related mortality. Results 96.10% of the total participants carried at least one PTV. We identified 866 PTVs that were positively associated with liver dysfunction at the threshold of P value < 8.21e - 07. The coding genes of these PTVs were mainly enriched in pathways related to lipid, fatty acid, amino acid, and carbohydrate metabolisms. The 866 PTVs were presented in 1.07% (4721) of participants. Compared with participants who did not carry any of the PTVs, the carriers had a 5.33-fold (95% CI 4.15-6.85), 2.82-fold (1.69-4.72), and 4.41-fold (3.04-6.41) increased risk for fibrosis and cirrhosis of liver, liver cancer, and liver disease-related mortality, respectively. These adverse effects were consistent across subgroups based on age, sex, body mass index, smoking status, and presence of hypertension, diabetes, dyslipidemia, and metabolic syndrome. Conclusions Our findings revealed a significant impact of PTVs in MT-related genes on liver disease risk, highlighting the importance of these variants in identifying populations at risk of liver diseases and facilitating early clinical interventions. Keywords: Protein truncating variant, Mitochondrial dysfunction, Liver-related biomarkers, Chronic liver disease, Genetic susceptibility
Vegetation mapping, identifying the type and distribution of plant species, is important for analysing vegetation dynamics, quantifying spatial patterns of vegetation evolution, analysing the effects ...of environmental changes and predicting spatial patterns of species diversity. Such analysis can contribute to the development of targeted land management actions that maintain biodiversity and ecological functions. This paper presents a methodology for 3D vegetation mapping of a coastal dune complex using a multispectral camera mounted on an unmanned aerial system with particular reference to the Buckroney dune complex in Co. Wicklow, Ireland. Unmanned aerial systems (UAS), also known as unmanned aerial vehicles (UAV) or drones, have enabled high-resolution and high-accuracy ground-based data to be gathered quickly and easily on-site. The Sequoia multispectral sensor used in this study has green, red, red edge and near-infrared wavebands, and a regular camer with red, green and blue wavebands (RGB camera), to capture both visible and near-infrared (NIR) imagery of the land surface. The workflow of 3D vegetation mapping of the study site included establishing coordinated ground control points, planning the flight mission and camera parameters, acquiring the imagery, processing the image data and performing features classification. The data processing outcomes included an orthomosaic model, a 3D surface model and multispectral imagery of the study site, in the Irish Transverse Mercator (ITM) coordinate system. The planimetric resolution of the RGB sensor-based outcomes was 0.024 m while multispectral sensor-based outcomes had a planimetric resolution of 0.096 m. High-resolution vegetation mapping was successfully generated from these data processing outcomes. There were 235 sample areas (1 m × 1 m) used for the accuracy assessment of the classification of the vegetation mapping. Feature classification was conducted using nine different classification strategies to examine the efficiency of multispectral sensor data for vegetation and contiguous land cover mapping. The nine classification strategies included combinations of spectral bands and vegetation indices. Results show classification accuracies, based on the nine different classification strategies, ranging from 52% to 75%.
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