•Crocus is a medicinally important plant and it is costliest spice of the world.•An efficient microprapogation protocol of five Turkish Crocus species was developed.•Crocus species: C. specious ssp. ...Specious, C. oliveri spp. Oliveri, C. pestalozzae, C. abantensis, and C. paschei..•Different combinations and concentrations of auxins and cytokinins were used.•Plant regeneration was developed via somatic embryogenesis.
Callus induction, somatic embryogenesis and plant regeneration were initiated in selected five species of Turkish crocus using three diffrent explants (leaf, stem and corm) cultured on four different media (MS, GB5, LS and CHE). The highest frequencies of callus induction (100%) and shoot regeneration (70%, with 7.2 shoots/callus) were found in the crocus species Crocus oliveri ssp. Oliveri, using the MS medium containing 5% (w/v) sucrose supplemented with (4mg/L NAA+4mg/L TDZ) and (2mg/L IAA+2mg/L TDZ+2mg/L BAP). When the embryogenic calli were transferred into the four nutrient media containing (2mg/L IAA+2mg/L TDZ) and 100mg/L ABA, these further developed into cotyledonary embryos. Maximum number of somatic embryos (2.9 embryos per leaf explant, with a frequency 46.6%) was obtained in C. oliveri ssp. Oliveri. During subculture using the half strength media, cotyledonary embryos gradually developed into plantlets.
The genus Quercus (Fagaceae) includes the most important woody plants with decidious and evergreen species in Northern hemisiphere. They have a problematic taxonomy because of widespread ...hybridization between the infrageneric taxa. Turkey is one of the most important region of the world according to oak species number and variation. In this study, species belonging to evergreen oaks in Turkey were investigated to solve taxonomic problems and to design the limit of taxa by using random amplified polymorphic DNA (RAPD) data. Here, three species of evergreen oaks known as Quercus coccifera, Quercus ilex and Quercus aucheri were studied in all area located and made the comparison within and among species studied using ten RAPD markers. As a result; it can be stated that the presence of the three species in Ilex section is clear. Furthermore, existence of two infraspecific taxa or two seperate taxa in species level within Q. coccifera may be quite possibly considered.
The genus Quercus L. has a problematic taxonomy because of widespreadhybridization among them. Evergreen Quercus contain three species in section Ilex Loudon namely, Q. ilex L., Q. coccifera L. and ...Q. aucheri Jaub. et Spach in Turkey. Here, two species, Q. coccifera and Q. aucheri are usually confused with each other. However, Q. coccifera and Q. calliprinos are accepted as different species but this subject is still controversial. Morphometric leaf and fruit variations of Q. ilex, Q. coccifera and Q. aucheri in 26 populations were measured for 25 characters. Variations within and among populations of species were detected by cluster analysis and principal component analysis. This study shows that populations of Q. coccifera from the south region of Turkey form a second group within Q. coccifera. Secondly, Q. coccifera show more similarity to Q. aucheri than Q. ilex, and finally there are two groups within Q. coccifera, which may be evaluated as Q. coccifera and Q. calliprinos.Keywords: Ilex; Morphometric, UPGMA; Turkey.Bangladesh J. Plant Taxon. 24(1): 3947, 2017 (June)
In this study; the two Crocus L. taxa endemic to Bolu province, Crocus abantensis T. Baytop et Mathew and Crocus× paulineae Pasche & Kerndorff (hybrid) together with C. ancyrensis (Herbert) Maw ...subsp. ancyrensis, and C. olivieri J.Gay, were used to reveal their detailed leaf anatomical features. In view of these characteristics, it was aimed to determine the true parents of the hybrid and possible other hybrid taxa distributed in the south-southeast coasts Abant Lake. These two Crocus taxa, which are endemic to this region, have no previous anatomical studies. In this sense, deficiencies related to Crocus taxonomy have been completed. The main differences of C. × paulineae from the other taxa; having the thickest cuticle (3.80 µm), the longest parenchyma (20.93 µm) cell in the mesophyll, and having papillae like structure on the keel corners of cuticle. This structure was also observed in C. olivieri over the cuticle at the corners of the keel. The other important differences was the number of small vascular bundles among the studied taxa. The chromosomal number of hybrid was also given for the first time.
Bu çalışmada; Bolu ili için endemik iki Crocus L. taksonu, Crocus abantensis T. Baytop et Mathew ve Crocus× paulineae Pasche & Kerndorff (melez), ile birlikte C. ancyrensis (Herbert) Maw subsp. ancyrensis ve C. olivieri J.Gay, Bull. ayrıntılı yaprak anatomik özelliklerini ortaya çıkarmak için kullanıldı. Bu özellikler ışığında Abant Gölü'nün Güney-Güneydoğu kıyısında yayılış gösteren hibrit ve olası diğer hibrit taksonların gerçek ebeveynlerinin belirlenmesi amaçlanmıştır. Bu bölgeye endemik olan bu iki Crocus taksonun daha önce anatomik çalışması yoktur. Bu anlamda Crocus taksonomisi ile ilgili eksiklikleri tamamlanmıştır. C. × paulineae’nın diğer taksonlardan temel farklılıkları; mezofildeki en kalın kütikül (3.80 µm), en uzun parankima (20.93 µm) hücresine ve kütikülün karina köşelerinde papilla benzeri bir yapıya sahip olmasıdır. Bu yapı C. olivieri de de gözlenmiştir. Diğer önemli farklılıklar, incelenen taksonlar arasındaki küçük damar demetlerinin sayısıdır. Hibritin kromozom sayısı da ilk kez verilmiştir.
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