Wearable sensors are increasingly finding their way into applications of kinesthetic sensing, personal health monitoring, and smart prosthetics/robotics. A graphene‐based composite fiber sensor with ...a “compression spring” structure is fabricated, featuring the ability of detecting multiple kinds of deformation. This fiber sensor is integrated into wearable sensors for monitoring human activities and intricate movements of robotics successfully.
As the fifth global water footprint assessment, this study enhanced previous estimates of national blue water consumption (including fresh surface and groundwater) and main economic activities with ...(1) improved spatial and sectoral resolution and (2) quantified the impacts of virtual water trade on water use and water stress at both the national and basin level. In 2007, 1194 Gm3 of blue water was consumed globally for human purposes. The consuming (producing) of primary and manufactured goods and services from the sectors of “Primary Crops and Livestock”, “Primary Energy and Minerals”, “Processed Food and Beverages”, “Non-food Manufactured Products”, “Electricity”, “Commercial and Public Services”, and “Households” accounted for 33% (91%), ∼ 0% (1%), 37% (<1%), 13% (1%), 1% (2%), 15% (3%), and 2% (2%) of the world’s total blue water consumption, respectively. The considerable differences in sectoral water consumption accounted for by the two perspectives (consumption- vs production-based) highlight the significance of the water consumed indirectly, upstream in the supply chain (i.e., > 70% of total blue water consumption) while offering additional insights into the water implications of critical interconnected economic activities, such as the water-energy nexus. With 145 Gm3 (12%) of the blue water consumption embedded in the goods and services traded internationally, 89 countries analyzed were net blue water importers at the national level. On the basin level, the impacts of virtual water trade on water stress were statistically significant for basins across the world and within 104 countries; virtual water trade mitigated water stress for the basins within 85 of the 104 countries, including all of those where there are moderate and greater water stress countrywide (except Italy).
Stretchable electronics, as a promising research frontier, has achieved progress in a variety of sophisticated applications. The realization of stretchable electronics frequently involves the demand ...for a stretchable conductor as an electrical circuit. However, it still remains a challenge to fabricate high-performance (working strain exceeding 200%) stretchable conductors. Here, we present for the first time a facile, cost-effective, and scalable method for manufacturing ultrastretchable composite fibers with a “twining spring” configuration: cotton fibers twining spirally around a polyurethane fiber. The composite fiber possesses a high conductivity up to 4018 S/cm, which remains as high as 688 S/cm at 500% tensile strain. In addition, the conductivity of the composite fiber (initial conductivity of 4018 S/cm) remains perfectly stable after 1000 bending events and levels off at 183 S/cm after 1000 cyclic stretching events of 200% strain. Stretchable LED arrays are integrated efficiently utilizing the composite fibers as a stretchable electric wiring system, demonstrating the potential applications in large-area stretchable electronics. The biocompatibility of the composite fiber is verified, opening up its prospects in the field of implantable devices. Our fabrication strategy is also versatile for the preparation of other specially functionalized composite fibers with superb stretchability.
Adaptive tendril coiling of climbing plants has long inspired the artificial soft microsystem for actuation and morphing. The current bionic research efforts on tendril coiling focus on either the ...preparation of materials with the coiling geometry or the design of self-shaping materials. However, the realization of two key functional features of the tendril, the spring-like buffering connection and the axial contraction, remains elusive. Herein, we devise a conductive tendril by fusing conductive yarns into tendril configuration, bypassing the prevailing conductivity constraints and mechanical limitations. The conductive tendril not only inherits an electrophysiology buffering mechanics with exceptional conductance retention ability against extreme stretching but also exhibits excellent contractive actuation performance. The integrative design of the ultraelastic conductive tendril shows a combination of compliant mobility, actuation, and sensory capabilities. Such smart biomimetic material holds great prospects in the fields of ultrastretchable electronics, artificial muscles, and wearable bioelectronic therapeutics.
NUT midline carcinoma (NMC) is a rare but highly aggressive cancer typically caused by the translocation t(15;19), which results in the formation of the BRD4-NUT fusion oncoprotein. Previous studies ...have demonstrated that fusion of the NUT protein with the double bromodomains of BRD4 may significantly alter the cellular gene expression profile to contribute to NMC tumorigenesis. However, the mechanistic details of this BRD4-NUT function remain poorly understood. In this study, we examined the NUT function in transcriptional regulation by targeting it to a LacO transgene array integrated in U2OS 2-6-3 cells, which allow us to visualize how NUT alters the in situ gene transcription dynamic. Using this system, we demonstrated that the NUT protein tethered to the LacO locus recruits p300/CREB-binding protein (CBP), induces histone hyperacetylation, and enriches BRD4 to the transgene array chromatin foci. We also discovered that, in BRD4-NUT expressed in NMC cells, the NUT moiety of the fusion protein anchored to chromatin by the double bromodomains also stimulates histone hyperacetylation, which causes BRD4 to bind tighter to chromatin. Consequently, multiple BRD4-interacting factors are recruited to the NUT-associated chromatin locus to activate in situ transgene expression. This gene transcription function was repressed by either expression of a dominant negative inhibitor of the p300-NUT interaction or treatment with (+)-JQ1, which dissociates BRD4 from the LacO chromatin locus. Our data support a model in which BRD4-NUT-stimulated histone hyperacetylation recruits additional BRD4 and interacting partners to support transcriptional activation, which underlies the BRD4-NUT oncogenic mechanism in NMC.
Background: NUT midline carcinoma is an aggressive cancer typically caused by the formation of the BRD4-NUT fusion oncoprotein.
Results: BRD4-NUT-stimulated histone hyperacetylation recruits BRD4 and associated transcription factors to activate gene expression.
Conclusion:BRD4-NUT perturbs normal gene expression to trigger oncogenic events in NMC.
Significance: Breaking BRD4-NUT interaction with histone acetyltransferases is an excellent strategy to abrogate the BRD4-NUT oncogenic activities.
A consequential life cycle assessment (LCA) is conducted to evaluate the trade-offs between water quality improvements and the incremental climate, resource, and economic costs of implementing green ...(bioretention basin, green roof, and permeable pavement) versus gray (municipal separate stormwater sewer systems, MS4) alternatives of stormwater infrastructure expansions against a baseline combined sewer system with combined sewer overflows in a typical Northeast US watershed for typical, dry, and wet years. Results show that bioretention basins can achieve water quality improvement goals (e.g., mitigating freshwater eutrophication) for the least climate and economic costs of 61 kg CO2 eq. and $98 per kg P eq. reduction, respectively. MS4 demonstrates the minimum life cycle fossil energy use of 42 kg oil eq. per kg P eq. reduction. When integrated with the expansion in stormwater infrastructure, implementation of advanced wastewater treatment processes can further reduce the impact of stormwater runoff on aquatic environment at a minimal environmental cost (77 kg CO2 eq. per kg P eq. reduction), which provides support and valuable insights for the further development of integrated management of stormwater and wastewater. The consideration of critical model parameters (i.e., precipitation intensity, land imperviousness, and infrastructure life expectancy) highlighted the importance and implications of varying local conditions and infrastructure characteristics on the costs and benefits of stormwater management. Of particular note is that the impact of MS4 on the local aquatic environment is highly dependent on local runoff quality indicating that a combined system of green infrastructure prior to MS4 potentially provides a more cost-effective improvement to local water quality.
In spite of the recent successful demonstrations of flexible and transparent film heaters, most heaters with high optical transmittance and low applied direct current （DC） voltage are silver nanowire ...（Ag NW）-based or silver grid-based. In this study, flexible and stretchable copper nanowire （Cu NW）-based transparent film heaters were fabricated through a solution-based process, in which a thin layer of hydrophobic polymers was encapsulated on the Cu NW films. The thin polymer layer protected the films from oxidation under harsh testing conditions, i.e., high temperature, high humidity, and acidic and alkaline environments. The films exhibited remarkable performance, a wide operating temperature range （up to 150 ℃）, and a high heating rate （14 ℃/s）. Defrosting and wearable thermotherapy demonstrations of the Cu NW film heaters were carried out to investigate their practicality. The Cu NW-based film heaters have potential as reliable and low-cost film heaters.
Increased water demand and scarce freshwater resources have forced communities to seek nontraditional water sources. These challenges are exacerbated in coastal communities, where population growth ...rates and densities in the United States are the highest. To understand the current management dilemma between constrained surface and groundwater sources and potential new water sources, Tampa Bay, Florida (TB), and San Diego, California (SD), were studied through 2030 accounting for changes in population, water demand, and electricity grid mix. These locations were chosen on the basis of their similar populations, land areas, economies, and water consumption characters as well as their coastal locations and rising contradictions between water demand and supply. Three scenarios were evaluated for each study area: (1) maximization of traditional supplies; (2) maximization of seawater desalination; and (3) maximization of nonpotable water reclamation. Three types of impacts were assessed: embodied energy, greenhouse gas (GHG) emission, and energy cost. SD was found to have higher embodied energy and energy cost but lower GHG emission than TB in most of its water infrastructure systems because of the differences between the electricity grid mixes and water resources of the two regions. Maximizing water reclamation was found to be better than increasing either traditional supplies or seawater desalination in both regions in terms of the three impact categories. The results further imply the importance of assessing the energy–water nexus when pursuing demand-side control targets or goals as well to ensure that the potentially most economical options are considered.
This paper examines the short-term and mid-term impact of COVID-19 restrictions on SMEs, based on two waves of phone interviews with a previously surveyed large SME sample in China. The outbreak of ...COVID-19 and the resultant lockdowns took a heavy toll on SMEs. Afflicted by problems of logistics blocks, labor shortages, and drops in demand, 80% of SMEs were temporarily closed at the time of the first wave of interviews in February 2020. After reining in COVID-19, authorities largely eased lockdown restrictions in April. Consequently, most SMEs had reopened by the time of the second round of surveys in May. However, many firms, particularly export firms, were running at partial capacity, primarily due to inadequate demand. Moreover, around 19% of incorporated enterprises and 25% of self-employed businesses had permanently closed between the two waves of surveys.
•We conducted two waves of large-scale phone surveys with SMEs in China.•COVID-19 landed a heavy toll on SMEs.•The challenges facing SMEs have changed from the supply side to the demand side.•More inclusive policies were more effective in helping SMEs than other targeted policies.
Neovascularization and vascular remodeling are functionally important for brain repair after stroke. We show that neutrophils accumulate in the peri-infarct cortex during all stages of ischemic ...stroke. Neutrophils producing intravascular and intraparenchymal neutrophil extracellular traps (NETs) peak at 3-5 days. Neutrophil depletion reduces blood-brain barrier (BBB) breakdown and enhances neovascularization at 14 days. Peptidylarginine deiminase 4 (PAD4), an enzyme essential for NET formation, is upregulated in peri-ischemic brains. Overexpression of PAD4 induces an increase in NET formation that is accompanied by reduced neovascularization and increased BBB damage. Disruption of NETs by DNase 1 and inhibition of NET formation by genetic ablation or pharmacologic inhibition of PAD increases neovascularization and vascular repair and improves functional recovery. Furthermore, PAD inhibition reduces stroke-induced STING-mediated production of IFN-β, and STING knockdown and IFN receptor-neutralizing antibody treatment reduces BBB breakdown and increases vascular plasticity. Collectively, our results indicate that NET release impairs vascular remodeling during stroke recovery.
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