Summary
Repair of
DNA
double‐strand breaks (
DSB
s) by recombination pathways is essential for plant growth and fertility. The recombination endonuclease
MRE
11 plays important roles in sensing and ...repair of
DNA DSB
s. Here we demonstrate protein interaction between Arabidopsis
MRE
11 and the histone acetyltransferase
TAF
1, a
TATA
‐binding protein Associated Factor (
TAF
) of the
RNA
polymerase
II
transcription initiation factor complex
TFIID
. Arabidopsis has two
TAF
1 homologues termed
TAF
1 and
TAF
1b and mutant
taf1b
lines are viable and fertile. In contrast,
taf1
null mutations are lethal, demonstrating that
TAF
1
is an essential gene. Heterozygous
taf1
+/−
plants display abnormal segregation of the mutant allele resulting from defects in pollen tube development, indicating that
TAF
1 is important for gamete viability. Characterization of an allelic series of
taf1
lines revealed that hypomorphic mutants are viable but display developmental defects and reduced plant fertility. Hypersensitivity of
taf1
mutants lacking the C‐terminal bromodomain to X‐rays and mitomycin C, but not to other forms of abiotic stress, established a specific role for
TAF
1 in plant
DNA
repair processes. Collectively these studies reveal a function for
TAF
1 in plant resistance to genotoxic stress, providing further insight into the molecular mechanisms of the
DNA
damage response in plants.
Significance Statement
DNA
recombination pathways are critical for genome stability, fertility and survival of organisms. Here we establish a molecular link between a recombination endonuclease and a histone acetyltransferase involved in transcription initiation, providing insight into chromosomal break detection and
DNA
repair processes.
Featured Cover Taylor, Rachel E.; West, Christopher E.; Foyer, Christine H.
Food and energy security,
March 2023, 2023-03-00, 20230301, Volume:
12, Issue:
2
Journal Article
Peer reviewed
Open access
The cover image is based on the Review Article WHIRLY protein functions in plants by Rachel E. Taylor et al., https://doi.org/10.1002/fes3.379
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