Hybrid organic-inorganic perovskite materials have obtained considerable attention due to their exotic optoelectronic properties and extraordinarily high performance in photovoltaic devices. Herein, ...we successively converted the ultrathin PbI2/MoS2 into the CH3NH3PbI3/MoS2 heterostructures via CH3NH3I vapor processing. Atomic force microscopy (AFM)、Scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS) measurements prove the high-quality of the converted CH3NH3PbI3/MoS2. Both MoS2 and CH3NH3PbI3 related photoluminescence (PL) intensity quenching in CH3NH3PbI3/MoS2 implies a Type-II energy level alignment at the interface. Temperature-dependent PL measurements show that the emission peak position shifting trend of CH3NH3PbI3 is opposite to that of MoS2 (traditional semiconductors) due to the thermal expansion and electron-phonon coupling effects. The CH3NH3PbI3/TMDC heterostructures are useful in fabricating innovative devices for wider optoelectronic applications.
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•Atomically thin CH3NH3PbI3 are templated by exfoliated MoS2 flakes in vacuum sequential deposition method.•Intensity quenching of both MoS2 and CH3NH3PbI3 PL peaks implies a Type-II energy level alignment at the interface.•Temperature-dependent emission properties of CH3NH3PbI3 are opposite to those of MoS2.
•Central body fat deposits are associated with poor vitamin D in school-aged children.•The relations of body fat deposits with vitamin D were more pronounced in boys.•Insulin resistance appears to ...play a role in these associations.
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To examine the association between regional fat deposits and vitamin D levels in Chinese children. Whether regional fat distribution contributes to vitamin D deficiency remains uncertain.
A population-based, nationwide and multicenter cross-sectional study involving 6,589 pediatric participants (49.9% boys) ages 6 to 18 y. The regional fat mass index (FMI) variables were calculated as regional fat mass (kg) divided by the square of height (m2), and plasma 25-hydroxyvitamin D (25OHD) levels were measured.
Among boys, after adjustment for multiple variables (including opposite regional fat), higher values for trunk FMI and android FMI were associated with a greater likelihood of vitamin D inadequacy (odds ratios ORs, 2.14 and 1.87, respectively), whereas the extremity FMI and gynoid FMI were associated with protection from vitamin D inadequacy (ORs, 0.63 and 0.71, respectively). Consistently, increased central fat distribution (e.g., trunk or android to total fat ratio) and decreased peripheral fat distribution (e.g., extremity or gynoid to total fat ratio) were associated with greater odds of vitamin D inadequacy. Among girls, the associations of regional fat with vitamin D were weaker than those in boys, and most analyses did not reach statistical significance.
Regional fat deposits were associated with vitamin D status in Chinese school-aged children and more pronounced among boys.
Colletotrichum gloeosporioides is a fungal disease of strawberry fruit. Biocontrol strategies holds tremendous promise in alleviating fruit decay. Here, 30 actinomycetes were isolated from ...rhizosphere soil of Calotropis gigantea. A strain labeled with CG-G2 exhibited the strongest antagonistic activity against C. gloeosporioides and was assigned as Streptomyces corchorusii. Compared to strain CG-G2 extracts, the volatile organic compounds (VOCs) had a high antifungal activity against anthracnose. These volatiles effectively inhibited mycelial growth and spore germination of C. gloeosporioides. The hyphal and conidial structure was severely destroyed. Metabolomics analysis revealed that VOCs inhibited C. gloeosporioides via inducing flavonoids metabolism contributing to antifungal activity. Three main antagonistic compounds in VOCs were identified as methyl 2-methyl butyrate, hexanenitrile and methyl 2-Ethyl hexanoate. Especially, methyl 2-methyl butyrate demonstrated a remarkable efficacy in inhibiting fruit decay and preserving fruit quality. Hence, S. corchorusii CG-G2 will be a potential biocontrol agent for controlling anthracnose on harvested fruits.
Banana anthracnose caused by Colletotrichum musae is a serious postharvest disease. Use of fungicides has potential adverse effects on the environment safety and human health. Application of ...biological control agents (BCA) is a promising strategy for managing postharvest fruit diseases. In this study, Streptomyces sp. XT34 was isolated from the banana rhizosphere soil and exhibited a strong antifungal activity against C. musae. Combining the phenotypic and whole-genomic alignment, strain XT34 was identified as Streptomyces diastatochromogenes. Strain XT34 extracts reduced the disease incidence of anthracnose and maintained the fruit quality of banana. Treatment of extracts reduced the colonization of C. musae on fruit surface. The activity inhibition of cell wall-degrading enzymes maintained the cell-wall strength of fruit. The activity increase of defense enzymes contributed to the low disease symptom. It was supported by the differentially expressed genes (DEGs) related to supramolecular fiber organization and flavonoid biosynthesis. In addition, spore germination of C. musae was effectively inhibited by extracts. The morphology of mycelia and spore become wrinkled and ruptured. The treatment of extracts increased the optical density of A260 and soluble sugar and protein contents of C. musae. The integrity of cell membrane was also destroyed, reflecting on an increase in electrical conductivity and malondialdehyde. Several metabolites were found in the genome of strain XT34 using antiSMASH. Eleven volatile compounds were identified by gas chromatography-mass spectrometer (GC-MS). Notably, strain XT34 and extracts exhibited a broad-spectrum antifungal activity against eight phytopathogenic fungi. Hence, S. diastatochromogenes XT34 provides a potential BCA to control banana anthracnose.
•Streptomyces XT34 exhibits strong antifungal activity against banana anthracnose.•Strain XT34 inhibits fruit decay and maintained quality in postharvest banana.•Strain XT34 extracts maintain cell-wall strength of fruit.•Extracts induce the biosynthesis of supramolecular fiber organization and flavonoid.•Strain XT34 extract inhibit pathogenic spore germination and caused cell rupture.
Disease-modifying passive immunotherapies focusing on removal of abnormal phosphorylated Tau (p-Tau) constitute promising treatments for Alzheimer’s disease (AD). Although several prior ...immunotherapies targeting p-Tau appear to be beneficial against AD, they have limitations such as the low blood-brain barrier (BBB) penetration rate, short half-life of antibodies, and the likelihood of inflammation. To address these issues, we designed a novel immunotherapy for AD. To this end, a single chain antibody (scFv) targeting p-Tau was generated, and a recombinant adeno-associated virus that can cross the BBB (rAAV/BBB) was used as a vector to express scFv for at least 22 weeks in the mouse brain. Results showed that the scFv constructed in this study had a high affinity to p-Tau and could bind to neuronal tangles in the section of brains of AD model mice. Moreover, the rAAV/BBB could cross the BBB, infect neuronal cells, and express scFv. This novel immunotherapy could effectively deliver scFv into the brain and resulted in a continuous expression of scFv in vivo, suggesting its potential for the treatment of AD.
•A novel immunotherapy for Alzheimer's disease was designed.•A scFv targeting p-Tau was expressed in rAAV-infected cells.•The rAAV mutant used can penetrate the blood brain barrier.•The p-Tau specific antibody showed prolonged expression in the brain.•The therapeutic effect of this immunotherapy will be investigated in AD mouse model.
Chronic pain is a major public health problem with limited effective therapeutic options. G-protein-coupled receptors play a significant role in pain modulation; however, whether and how ...G-protein-coupled receptor 183 participates in pain regulation remain unclear. In the present study, we found that G-protein-coupled receptor 183 expression was specifically upregulated in the hind paws of mice in various inflammatory pain models. Activation of G-protein-coupled receptor 183 induced acute pain, whereas inhibition or silencing of this receptor alleviated mechanical allodynia and thermal hyperalgesia in complete Freund's adjuvant (CFA) model. Mechanistically, activating G-protein-coupled receptor 183 triggers pain responses via the upregulation of C-C motif chemokine 22(CCL22) in macrophages while blocking the CCL22 receptor C-C motif chemokine receptor 4 (CCR4) attenuates pain hypersensitivity. Taken together, our findings indicate that the G-protein-coupled receptor 183-CCL22 axis has a critical role in the development and maintenance of inflammatory pain.
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In the field of near-infrared weak light detection, an InP/InGaAs single-photon avalanche diode (SPAD) is preferred due to the advantages of high sensitivity, low cost and room-temperature operation. ...To properly simulate and optimize the SPAD’s front-end circuit, a comprehensive and compact behavior model of the InP/InGaAs SPAD is normally required to accurately describe the statistical behavior of the detectors. In this paper, an InP/InGaAs SPAD analytical model is constructed, which not only includes the direct current (DC) and alternating current (AC) behavior simulating the avalanche and quenching processes, but also describes the dark count, after-pulsing and photon detection efficiency. For dark count noise, three important generation mechanisms are considered, including thermal generation, trap-assisted tunneling and band-to-band tunneling. The model described by the Verilog-A hardware description language (HDL) can be directly implemented in the commercial circuit simulator. A gated mode, passive quenching and recharging circuit is used to simulate and verify the developed model. The simulation results are in good agreement with the reported test data, demonstrating the accuracy of the developed InP/InGaAs SPAD model.
Banana Fusarium wilt caused by
Fusarium oxysporum
f. sp.
cubense
tropical race4 (
Foc
TR4) is one of the most destructive soil-borne fungal diseases and currently threatens banana production around ...the world. Until now, there is lack of an effective method to control banana Fusarium wilt. Therefore, it is urgent to find an effective and eco-friendly strategy against the fungal disease. In this study, a strain of
Trichoderma
sp. N4-3 was isolated newly from the rhizosphere soil of banana plants. The isolate was identified as
Trichoderma parareesei
through analysis of
TEF1
and
RPB2
genes as well as morphological characterization.
In vitro
antagonistic assay demonstrated that strain N4-3 had a broad-spectrum antifungal activity against ten selected phytopathogenic fungi. Especially, it demonstrated a strong antifungal activity against
Foc
TR4. The results of the dual culture assay indicated that strain N4-3 could grow rapidly during the pre-growth period, occupy the growth space, and secrete a series of cell wall-degrading enzymes upon interaction with
Foc
TR4. These enzymes contributed to the mycelial and spore destruction of the pathogenic fungus by hyperparasitism. Additionally, the sequenced genome proved that strain N4-3 contained 21 genes encoding chitinase and 26 genes encoding
β
-1,3-glucanase. The electron microscopy results showed that theses cell wall-degrading enzymes disrupted the mycelial, spore, and cell ultrastructure of
Foc
TR4. A pot experiment revealed that addition of strain N4-3 significantly reduced the amount of
Foc
TR4 in the rhizosphere soil of bananas at 60 days post inoculation. The disease index was decreased by 45.00% and the fresh weight was increased by 63.74% in comparison to the control. Hence,
Trichoderma parareesei
N4-3 will be a promising biological control agents for the management of plant fungal diseases.
Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc TR4) is the most destructive soil-borne fungal disease. Until now, there has been a lack of effective measures to control the ...disease. It is urgent to explore biocontrol agents to control Foc TR4 and the secretion of mycotoxin. In this study, fluvirucin B6 was screened from Streptomyces solisilvae using an activity-guided method. Fluvirucin B6 exhibited strong antifungal activity against Foc TR4 (0.084 mM of EC50 value) and significantly inhibited mycelial growth and spore germination. Further studies demonstrated that fluvirucin B6 could cause the functional loss of mitochondria, the disorder of metabolism of Foc TR4 cells, and the decrease of enzyme activities in the tricarboxylic acid cycle and electron transport chain, ultimately inhibiting mycotoxin metabolism. In a pot experiment, the application of fluvirucin B6 significantly decreased the incidence of banana Fusarium wilt and the amount of Foc TR4 and controlled fungal toxins in the soil. Additionally, fluvirucin B6 could positively regulate the changes in the structure of the banana rhizosphere microbial community, significantly enriching beneficial microbes associated with disease resistance. In summary, this study identifies fluvirucin B6, which plays versatile roles in managing fungal diseases and mycotoxins.Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc TR4) is the most destructive soil-borne fungal disease. Until now, there has been a lack of effective measures to control the disease. It is urgent to explore biocontrol agents to control Foc TR4 and the secretion of mycotoxin. In this study, fluvirucin B6 was screened from Streptomyces solisilvae using an activity-guided method. Fluvirucin B6 exhibited strong antifungal activity against Foc TR4 (0.084 mM of EC50 value) and significantly inhibited mycelial growth and spore germination. Further studies demonstrated that fluvirucin B6 could cause the functional loss of mitochondria, the disorder of metabolism of Foc TR4 cells, and the decrease of enzyme activities in the tricarboxylic acid cycle and electron transport chain, ultimately inhibiting mycotoxin metabolism. In a pot experiment, the application of fluvirucin B6 significantly decreased the incidence of banana Fusarium wilt and the amount of Foc TR4 and controlled fungal toxins in the soil. Additionally, fluvirucin B6 could positively regulate the changes in the structure of the banana rhizosphere microbial community, significantly enriching beneficial microbes associated with disease resistance. In summary, this study identifies fluvirucin B6, which plays versatile roles in managing fungal diseases and mycotoxins.
Overstocked dumping sites associated with open-pit coal mining occupy original vegetation areas and cause damage to the environment. The monitoring of vegetation disturbance and restoration at ...dumping sites is important for the accurate planning of ecological restoration in mining areas. This paper aimed to monitor and assess vegetation disturbance and restoration in the dumping sites of the Baorixile open-pit mine using the LandTrendr algorithm and remote sensing images. Firstly, based on the temporal datasets of Landsat from 1990 to 2021, the boundaries of the dumping sites in the Baorixile open-pit mine in Hulunbuir city were extracted. Secondly, the LandTrendr algorithm was used to identify the initial time and duration of vegetation disturbance and restoration, while the Normalized Difference Vegetation Index (NDVI) was used as the input parameter for the LandTrendr algorithm. Thirdly, the vegetation restoration effect at the dumping sites was monitored and analyzed from both temporal and spatial perspectives. The results showed that the dumping sites of the Baorixile open-pit mine were disturbed sharply by the mining activities. The North dumping site, the South dumping site, and the East dumping site (hereinafter referred to as the North site, the South site, and the East site) were established in 1999, 2006, and 2010, respectively. The restored areas were mainly concentrated in the South site, the East site, and the northwest of the North site. The average restoration intensity in the North site, South site, and East site was 0.515, 0.489, and 0.451, respectively, and the average disturbance intensity was 0.371, 0.398, and 0.320, respectively. The average restoration intensity in the three dumping sites was greater than the average disturbance intensity. This study demonstrates that the combination of temporal remote sensing images and the LandTrendr algorithm can follow the vegetation restoration process of an open-pit mine clearly and can be used to monitor the progress and quality of ecological restoration projects such as vegetation restoration in mining areas. It provides important data and support for accurate ecological restoration in mining areas.
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