Here, in an analysis of a 2.92 fb–1 data sample taken at 3.773 GeV with the BESIII detector operated at the BEPCII collider, we measure the absolute decay branching fractions to be B(D0 → K–e+νe) = ...(3.505 ± 0.014 ± 0.033)% and B(D0 → π–e+νe) = (0.295 ± 0.004 ± 0.003)%. From a study of the differential decay rates we obtain the products of hadronic form factor and the magnitude of the CKM matrix element $f$ $^{K}_{+}$(0)|Vcs| = 0.7172 ± 0.0025 ± 0.0035 and $f$ $^{π}_{+}$(0)|Vcd| = 0.1435 ± 0.0018 ± 0.0009.
γ-aminobutyric acid (GABA) is a four-carbon non-protein amino acid presented in a wide range of organisms. In this study, a suppression subtractive hybridization (SSH) library was constructed using ...roots of a legume shrub, Caragana intermedia, with the combined treatment of 300 m m NaCl and 300 m m NaCl + 10 m m GABA. We obtained 224 GABA-regulated unique expressed sequence tags (ESTs) including signal transduction, transcriptional regulation, hormone biosynthesis, reactive oxygen species (ROS) and polyamine metabolism, etc. The key H₂O₂-generated genes, NADPH oxidase (CaGR60), peroxidase (CaGR61) and amine oxidase (CaGR62), were regulated at the mRNA level by 10 m m GABA, which clearly inhibited H₂O₂ accumulation brought about by NaCl stress in roots and leaves with the observation of 3,3′-diaminobenzidine (DAB) staining. Similarly, 10 m m GABA also regulated the expression of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) genes (CaGR30 and CaGR31) and ethylene production in NaCl-treated roots. Surprisingly, these H₂O₂-generated genes were enhanced at the mRNA level by a lower concentration of GABA, at 0.25 m m, but not other alternative nitrogen sources, and endogenous GABA accumulated largely just by the application of GABA at either concentration. Our results further proved that GABA, as a signal molecule, participates in regulating the expression of genes in plants under salt stress.
The shortage of strong endosperm-specific expression promoters for driving the expression of recombinant protein genes in cereal endosperm is a major limitation in obtaining the required level and ...pattern of expression. Six promoters of seed storage glutelin genes (GluA-1, GluA-2, GluA-3, GluB-3, GluB-5, and GluC) were isolated from rice (Oryza sativa L.) genomic DNA by PCR. Their spatial and temporal expression patterns and expression potential in stable transgenic rice plants were examined with β-glucuronidase (GUS) used as a reporter gene. All the promoters showed the expected spatial expression within the endosperm. The GluA-1, GluA-2, and GluA-3 promoters directed GUS expression mainly in the outer portion (peripheral region) of the endosperm. The GluB-5 and GluC promoters directed GUS expression in the whole endosperm, with the latter expressed almost evenly throughout the whole endosperm, a feature different from that of other rice glutelin gene promoters. The GluB-3 promoter directed GUS expression solely in aleurone and subaleurone layers. Promoter activities examined during seed maturation showed that the GluC promoter had much higher activity than the other promoters. These promoters are ideal candidates for achieving gene expression for multiple purposes in monocot endosperm but avoid promoter homology-based gene silencing. The GluC promoter did not contain the endosperm specificity-determining motifs GCN4, AACA, and the prolamin-box, which suggests the existence of additional regulatory mechanism in determining endosperm specificity.
The cross sections of e+e-→π+π-hc at center-of-mass energies from 3.896 to 4.600 GeV are measured using data samples collected with the BESIII detector operating at the Beijing Electron Positron ...Collider. The cross sections are found to be of the same order of magnitude as those of e+e-→π+π- J/ψ and e+e-→π+π-ψ (2S), but the line shape is inconsistent with the Y states observed in the latter two modes. Two structures are observed in the e+e- → π+π- hc cross sections around 4.22 and 4.39 GeV / c 2 , which we call Y ( 4220 ) and Y ( 4390 ) , respectively. A fit with a coherent sum of two Breit-Wigner functions results in a mass of (4218.4 $+5.5\atop{-4.5 ± 0.9) MeV/c2 and a width of 66.0$+12.3\atop-8.3$±0.4 MeV for the Y (4220), and a mass of (4391.5 $+6.3\atop-16.8$ ± 1.0) MeV/c2 and a width of (139.5$+16.2\atop-20.6 ± 0.6) MeV for the Y (4390), where the first uncertainties are statistical and the second ones systematic. The statistical significance of Y ( 4220 ) and Y(4390) is 10σ over one structure assumption.
In this paper, the spin and parity of the Zc(3900)± state are determined to be JP = 1+ with a statistical significance larger than 7σ over other quantum numbers in a partial wave analysis of the ...process e+e- → π+π-J/Ψ. We use a data sample of 1.92 fb-1 accumulated at $ \sqrt{s}=4.23 $ and 4.26 GeV with the BESIII experiment. When parametrizing the Zc(3900)± with a Flatté-like formula, we determine its pole mass Mpole = (3881.2±4.2stat ±52.7syst) MeV/c2 and pole width Γpole = (51.8± 4.6stat ± 36.0syst) MeV. Finally, we also measure cross sections for the process e+e- → Zc(3900)+π- + c.c. → J/Ψπ+π- and determine an upper limit at the 90% confidence level for the process e+e- → Zc(4020)+π- + c.c. → J/Ψ π+π-.
Using a total of 9.0 fb−1 of e+e− collision data with center-of-mass energies between 4.15 and 4.30 GeV collected by the BESIII detector, we search for the processes e+e−→γX(3872) with X(3872)→π0χcJ ...for J=0, 1, 2. We report the first observation of X(3872)→π0χc1, a new decay mode of the X(3872), with a statistical significance of more than 5σ for all systematic fit variations. Normalizing to the previously established process e+e−→γX(3872) with X(3872)→π+π−J/ψ, we find B(X(3872)→π0χc1)/B(X(3872)→π+π−J/ψ)=0.88−0.27+0.33±0.10, where the first error is statistical and the second is systematic. We set 90% confidence level upper limits on the corresponding ratios for the decays to π0χc0 and π0χc2 of 19 and 1.1, respectively.
Using a data sample of 448.1×106 ψ(3686) events collected with the BESIII detector operating at the BEPCII, we perform search for the hadronic transition hc→π+π−J/ψ via ψ(3686)→π0hc. No signals of ...the transition are observed, and the upper limit on the product branching fraction B(ψ(3686)→π0hc)B(hc→π+π−J/ψ) at the 90% confidence level (C.L.) is determined to be 2.0×10−6. This is the most stringent upper limit to date.
Using a data sample corresponding to an integrated luminosity of 2.93 fb−1 recorded by the BESIII detector at a center-of-mass energy of 3.773 GeV, we present an analysis of the decays D0 → π−π0e+νe ...and D+ → π−π+e+νe. By performing a partial wave analysis, the π+π− S-wave contribution to D+ → π−π+e+νe is observed to be (25.7 ± 1.6 ± 1.1)% with a statistical significance greater than 10σ, besides the dominant P-wave contribution. This is the first observation of the S-wave contribution. We measure the branching fractions B(D0 → ρ−e+νe) = (1.445 ± 0.058 ± 0.039) × 10−3, B(D+ → ρ0e+νe) = (1.860 ± 0.070 ± 0.061) × 10−3, and B(D+ → f0(500)e+νe, f0(500) → π+π−) = (6.30 ± 0.43 ± 0.32) × 10−4. An upper limit of B(D+ → f0(980)e+νe, f0(980) → π+π−) < 2.8 × 10−5 is set at the 90% confidence level. We also obtain the hadronic form factor ratios of D → ρe+νe at q2 = 0 assuming the single-pole dominance parametrization: rV = {V(0)/A1(0)} = 1.695 ± 0.083 ± 0.051, r2 = {A2(0)/A1(0)} = 0.845 ± 0.056 ± 0.039.