The black soldier fly, Hermetia illucens, is a cosmopolitan insect of the family Stratiomyidae (Diptera). Chemosensory genes encode proteins involved directly in the detection of odorants. In this ...study, we sequenced the antennal transcriptome of H. illucens adults to identify chemosensory genes. Putative unigenes encoding 27 odorant binding proteins (OBPs), five chemosensory proteins (CSPs), 70 odorant receptors (ORs), 25 ionotropic receptors (IRs), 10 gustatory receptors (GRs) and two sensory neuron membrane proteins (SNMPs) were identified. Tissue-specific expression profiles of the identified OBPs, CSPs and SNMPs were investigated using RT-PCR. Eight OBPs (HillOBP1-2, 9, 11-14, and 17), one CSP (HillCSP5) and one SNMP (HillSNMP1) were predominantly expressed in antennae. Further real-time quantitative PCR analyses revealed that the antennae-enriched unigenes also exhibited significant differences in expression between males and females. Among the sex-biased unigenes, six ORs showed female-biased expression, suggesting that these genes might participate in female-specific behaviors such as oviposition site searching. Sixteen ORs and two OBPs showed male-biased expression, indicating that they may play key roles in the detection of female sex pheromones. Our study is the first attempt to delineate the molecular basis of chemoreception in H. illucens. Our data provide useful information for comparative studies on the differentiation and evolution of Dipteran chemosensory gene families.The black soldier fly, Hermetia illucens, is a cosmopolitan insect of the family Stratiomyidae (Diptera). Chemosensory genes encode proteins involved directly in the detection of odorants. In this study, we sequenced the antennal transcriptome of H. illucens adults to identify chemosensory genes. Putative unigenes encoding 27 odorant binding proteins (OBPs), five chemosensory proteins (CSPs), 70 odorant receptors (ORs), 25 ionotropic receptors (IRs), 10 gustatory receptors (GRs) and two sensory neuron membrane proteins (SNMPs) were identified. Tissue-specific expression profiles of the identified OBPs, CSPs and SNMPs were investigated using RT-PCR. Eight OBPs (HillOBP1-2, 9, 11-14, and 17), one CSP (HillCSP5) and one SNMP (HillSNMP1) were predominantly expressed in antennae. Further real-time quantitative PCR analyses revealed that the antennae-enriched unigenes also exhibited significant differences in expression between males and females. Among the sex-biased unigenes, six ORs showed female-biased expression, suggesting that these genes might participate in female-specific behaviors such as oviposition site searching. Sixteen ORs and two OBPs showed male-biased expression, indicating that they may play key roles in the detection of female sex pheromones. Our study is the first attempt to delineate the molecular basis of chemoreception in H. illucens. Our data provide useful information for comparative studies on the differentiation and evolution of Dipteran chemosensory gene families.
Electromigration in Ge2Sb2Te5 and N-doped Ge2Sb2Te5 line cell structures has been studied by mapping out electric field/current-induced composition changes using x-ray energy dispersive spectroscopy. ...Both materials exhibit pronounced segregation in a molten state, with Te moving toward the anode and Ge and Sb toward the cathode. The width of the transition region from a composition of over 90% Te to over 90% Ge–Sb was 500 nm for an electric field of 1.1 × 107 V/m. In the Ge–Sb-rich end of the cell, Ge precipitates out of the melt, forming almost pure Ge inclusions with a size up to 100 nm. The Ge–Sb–Te segregation and precipitation do not appear to be affected by doping with nitrogen.
The elastic modulus, shear modulus, and Poisson’s ratio of timber are the elastic constants characterizing its material properties. In this paper, the transient excitation method was used to ...dynamically measure the 10 elastic constants of the falling larch wood under the condition of the free board and cantilever board, that is, 3 elastic moduli E, 3 shear moduli G, and 4 Poisson’s ratios μ. The other two Poisson’s ratios μ were derived using the principle of orthogonality. At the same time, the elastic modulus, shear modulus, and Poisson’s ratio under static conditions were tested and verified by symmetrical four-point bending, asymmetrical four-point bending, and tensile methods. This study is expected to have good application value and practical significance for timber as an engineering structural material, which is widely used in architecture, decoration, furniture, transportation, musical instruments, and in other fields.
This paper proposes an improvement in the test method for determining the flexural dynamic modulus of elasticity of strawboard with two triangular prisms as supports, for quality control and ...classification. Free-plate modal and free-plate transient excitation methods were used to test the elastic modulus of 1/4-plate and whole-plate strawboards. The dynamic test results were verified with the four-point bending method and tensile method. The results show that the elastic moduli of strawboards is approximately 2 GPa. The dynamic test method proposed is efficient, simple, repeatable, and accurate. This method is more suitable for factory applications than existing dynamic testing methods. The framed cases produced by the strawboard all meet the performance requirements in GB/T 7284 (2016).
Organic fertilizer is utilized to improve the organic carbon levels in arable soils, which is helpful for soil quality improvement and crop yield increase. However, the after-effect of organic ...fertilizer varies among regions with different temperature and precipitation conditions, and the extent of the impact remains unknown. This study aimed to investigate the impact of varying temperature and rainfall conditions on the accumulation of soil organic carbon after organic fertilizer application. A meta-analysis of 168 peer-reviewed studies published between 2005 and 2022 involving a total of 464 trials was conducted. The following was discovered: (1) In the major grain-producing areas of China, there was a significant positive correlation (p < 0.01) between latitude and soil organic carbon content. Meanwhile, temperature and precipitation had a significant negative correlation (p < 0.01) with soil organic carbon content. (2) The increase in temperature inhibited the increase in soil organic carbon storage. The improvement effect of organic fertilizer application in the low-temperature areas was significantly increased by 60.93% compared with the mid-temperature areas, and by 69.85% compared with the high-temperature areas. The average annual precipitation affected the after-effect of organic fertilizer as follows: 400–800 mm > 400 mm > more than 800 mm. (3) The influence of climatic conditions on the after-effect of organic fertilizer was more significant depending on the specific tillage practice. To increase organic fertilizer use efficiency and eliminate greenhouse gas emissions, liquid organic fertilizers with abundant trace nutrients and amino acids, which take advantage of releasing nutrients more swiftly and have a better fertilization effect, could be an alternative to traditional organic fertilizers.
Various impact monitoring systems have been proposed based on wireless sensor network (WSN) in the past decades. However, most of the research only concentrated on the developments of wireless sensor ...nodes instead of networks. For real aircraft applications, WSN-based impact monitoring with multiple nodes is necessary for large-scale structures. Under this situation, WSN-based globe impact localization algorithm is an important issue to be addressed, yet few reports can be found so far. Meanwhile, real aircraft composites usually have complex styles that can degrade the performance of monitoring algorithms, which is vital for successful applications. Hence, in this article, a global impact localization method with a large-scale Bluetooth-based piezoelectric transducer (PZT) sensor network is proposed. Two impact localization algorithms are comprehensively researched and evaluated on a complex aircraft panel structure. Furthermore, a real aircraft wing box structure with multiple nodes is adopted for an overall evaluation of the network. Satisfying results are achieved.
In recent years, the rice-crayfish coculture has shown significant progress as an agricultural technology with significant economic value in the middle and lower reaches of the Yangtze River in ...China. Nonetheless, as the promotion of the technology expands, the unvarying co-culture method poses challenges in adapting to the diverse environmental conditions across different regions. This has resulted in regional disparities dominated by environmental factors, which have emerged as the primary obstacle to the advancement of rice-crayfish coculture.
It is uncertain whether the rice-crayfish coculture model can be implemented in tropical regions due to the higher temperature and stronger solar radiation. Therefore, this study analyzes the feasibility of establishing the rice-crayfish coculture model through estimating the rice and crayfish annual production and the economic and ecological benefits in the tropical region.
The growth, annual production, and nitrogen partial factor productivity (NPFP) of rice, nitrogen dynamics in the field water, as well as the economic inputs and benefits associated with the rice-crayfish coculture system over two years were evaluated in Hainan province.
It was found that: 1) Even with a reduction of 33.3% in nitrogen fertilizer application, the annual production of the rice-crayfish coculture system remains equivalent to that of the rice monoculture system. Furthermore, the rice-crayfish coculture can increase the effective panicle number of rice. 2) The rice-crayfish coculture system can provide nutrients to support rice growth without polluting the water environment. The nitrogen fertilizer partial productivity in the rice-crayfish coculture system is significantly higher than that in the rice monoculture system. Crayfish culture introduced additional nitrogen in rice field at early stage, but it did not gain any form of nitrogen content in the field water at the end of the season. 3) In comparison to the rice monoculture system, the tropical rice-crayfish coculture system increased net profit by 152.82% -745.26%. 4) Comprehensive analyses of the growth, yield, and economic benefits showed that SXHN is better than YXYLS, which indicated that suitable varieties were needed for developing rice-crayfish coculture in the tropics.
Rice-crayfish coculture is an economic and ecological agricultural system. However, rice-crayfish coculture encounters extensive technical challenges across various regions, because of the varied culture conditions. These challenges impede the expansion of the rice-crayfish coculture model. Therefore, this study aimed to evaluate the practicality of the rice-crayfish coculture system in the tropics to facilitate the development of the rice-crayfish coculture model.
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•The development of rice-crayfish coculture system in tropical regions is feasible and profitable.•The difference in annual production between the two systems was not significant.•The NPFP of the rice-crayfish coculture system is significantly higher than that of the rice monoculture system.•Compared to the rice monoculture, the net profit of the rice-crayfish coculture system can increase by 152.82% to 745.26%.•SXHN is better suited to rice-crayfish coculture in the tropics, as compared with YXYLS.
The elastic modulus, shear modulus, and Poisson's ratio of timber are the elastic constants characterizing its material properties. In this paper, the transient excitation method was used to ...dynamically measure the 10 elastic constants of the falling larch wood under the condition of the free board and cantilever board, that is, 3 elastic moduli E, 3 shear moduli G, and 4 Poisson's ratios μ. The other two Poisson's ratios μ were derived using the principle of orthogonality. At the same time, the elastic modulus, shear modulus, and Poisson's ratio under static conditions were tested and verified by symmetrical four-point bending, asymmetrical four-point bending, and tensile methods. This study is expected to have good application value and practical significance for timber as an engineering structural material, which is widely used in architecture, decoration, furniture, transportation, musical instruments, and in other fields.
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