Abstract By analyzing 4.48 × 108 ψ(3686) events collected with the BESIII detector, we observe the decays χ cJ → nK S 0 Λ ¯ $$ {nK}_S^0\overline{\Lambda} $$ + c.c. (J = 0, 1, 2) for the first time, ...via the radiative transition ψ(3686) → γχ cJ . The branching fractions are determined to be (6.65 ± 0.26stat ± 0.41syst) × 10 −4, (1.66 ± 0.12stat ± 0.12syst) × 10 −4, and (3.58 ± 0.16stat ± 0.23syst) × 10 −4 for J = 0, 1, and 2, respectively.
Osteogenesis imperfecta (OI) is a group of hereditary disorders characterized by low bone mass and recurrent fractures. OI patients of autosomal recessive inheritance are extremely rare, of which OI ...type XIII is attributable to mutation in BMP1 gene.
Here, we detect the pathogenic mutations and analyze their relation to the phenotypes in a Chinese family with OI using next-generation sequencing (NGS) and Sanger sequencing. We also evaluate the efficacy of alendronate treatment in the patient with OI type XIII. The clinical phenotypes of the patient included recurrent fractures, muscle weakness, bone deformity, macrocephaly and elbow contractures, but no blue sclera or dentinogenesis imperfecta. High-resolution peripheral quantitative computed tomography revealed high bone mineral density and bone volume, but reduced trabecular numbers, increased porosity and comprised strength in this patient. Novel heterozygous mutations of c.1324G > T (p.Asp442Tyr) and c.148 + 1G > A in BMP1 gene were found in the proband, which would affect the CUB2 domain and the prodomain of mutant proteins. The parents were heterozygous carriers for the two mutations respectively, but with normal phenotype.
We report for the first time that the novel pathogenic mutations in BMP1 can lead to the extremely rare OI type XIII, which exhibit unique characters of high bone mass, but with impaired bone microstructure and comprised bone strength. Alendronate is beneficial in increasing bone mineral density and decreasing bone resorption biomarkers, but concerns still remain whether it can reduce fracture incidence in this rare type of OI.
•We identify two novel heterozygous variants in BMP1 in a Chinese family with autosomal-recessive OI.•HR-pQCT revealed high bone mass, but impaired microstructure and comprised strength in patient with BMP1 mutations.•The anti-fracture efficancy of bisphosphonates is unclear in this rare type of OI.
Gastric cancer is the third most common cause of cancer-related deaths and is the fifth highest incidence of cancer worldwide, especially in Eastern Asia, Central and Eastern Europe, and South ...America. Currently, surgery is the only curative treatment for gastric cancer; however, digestive tract reconstruction after distal gastrectomy for gastric cancer is controversial due to the postoperative complications such as reflux gastritis. There is an increasing trend toward laparoscopic uncut Roux-en-Y (URY) for radical gastrectomy. However, evidence on the feasibility of this procedure in patients undergoing laparoscopic radical distal gastrectomy is still absent. Thus, a prospective randomized trial is warranted. This is a prospective, multicenter, two-arm randomized controlled trial in which 210 patients will be randomly assigned to two groups: laparoscopic URY (n=105) and laparoscopic Billroth II plus Braun anastomosis (n=105). Each participant must be pathologically diagnosed with gastric cancer and undergo laparoscopic radical gastrectomy at Xijing Hospital and other four hospitals. The laparoscopic URY procedure is based on the Billroth II gastrojejunostomy plus Braun anastomosis, and then blocked the jejunum input loop at the stump-jejunal anastomosis. The patients' demographic and pathological characteristics will be recorded. The total and oral nutritional intake, general data, total serum protein, serum albumin, blood glucose, and temperature will be recorded before surgery and at the time of hospitalization. Postoperative adverse events will also be recorded, as well as at follow-up appointments at three months and six months after surgery. The rate of reflux gastritis will represent the primary endpoint, and other secondary endpoints, which are all recorded.
Au colloids were used to fabricate nanoscale-tunable Au nanofilms on silicon for surface-enhanced IR absorption bases in both ambient and electrochemical environments. This wet process incorporates ...the self-assembly of colloidal Au monolayer using 3-aminopropyl trimethoxysilane as the organic coupler with subsequent chemical plating in an Au(III)/hydroxylamine solution. FTIR spectroscopy in transmission mode of the probe species SCN- was used to evaluate the apparent surface enhancement in IR absorption of 2D Au colloid arrays and chemically plated Au particles. The nanostructure of Au films was examined by atomic force microscopy. The IR and AFM results show that the apparent surface enhancement factor (1−2 orders of magnitude) increases with increasing sizes and/or contact, and the severe aggregation of Au nanoparticles may cause the bipolar band shape. Cyclic voltammetry on the Au nanofilm obtained by the above nucleation and growth strategy exhibits a feasible electrochemical stability and behavior. In situ ATR-FTIR measurement of p-nitrobenzoic acid adsorption demonstrates that the as-grown Au film yields rather promising surface enhancement as well.
Abstract With data samples collected with the BESIII detector at seven energy points at s $$ \sqrt{s} $$ = 3.68 − 3.71 GeV, corresponding to an integrated luminosity of 333 pb −1, we present a study ...of the Λ transverse polarization in the e + e − → Λ Λ ¯ $$ \Lambda \overline{\Lambda} $$ reaction. The significance of polarization by combining the seven energy points is found to be 2.6σ including the systematic uncertainty, which implies a non-zero phase between the transition amplitudes of the Λ Λ ¯ $$ \Lambda \overline{\Lambda} $$ helicity states. The modulus ratio and the relative phase of EM-psionic form factors combined with all energy points are measured to be R Ψ = 0.71 − 0.10 + 0.10 $$ {0.71}_{-0.10}^{+0.10} $$ ± 0.03 and ∆ΦΨ = 23 − 8.0 + 8.8 $$ {23}_{-8.0}^{+8.8} $$ ± 1.6°, where the first uncertainties are statistical and the second systematic.
Surface-enhanced infrared absorption spectroscopy (SEIRAS) in the attenuated-total-reflection configuration (ATR-SEIRAS) has been applied for the first time to Ni electrodes. SEIRA-active Ni ...electrodes were obtained through initial chemical deposition of a 60-nm-thick Au underlayer on the reflecting plane of an ATR Si prism followed by potentiostatic electrodeposition of a 40-nm-thick Ni overlayer in a modified Watt's electrolyte. The Ni nanoparticle film thus obtained exhibited exceptionally enhanced IR absorption for the surface probe molecule CO while maintaining unipolar and normally directed bands. With the advantages of ATR-SEIRAS, free H2O molecules coadsorbed with CO at the Ni electrode were revealed, and their role in the electrooxidation of the CO adlayer at the Ni electrode is discussed. In addition, the conversion of bridge to linearly bonded CO at Ni electrode in a neutral solution was clearly identified upon electrooxidation of the CO adlayer. ATR-SEIRAS was also used to characterize the adsorption configuration of a pyridine adlayer at the Ni electrode. Both A1 and B1 modes of adsorbed pyridine were detected with comparably large intensities, essentially maintaining the spectral feature of pyridine molecules rather than that of “α-pyridyl species”, which strongly suggests an “edge-tilted pyridine” configuration present at the Ni electrode, a configuration intermediate between the “end-on pyridine” and “edge-on α-pyridyl” adsorption modes reported in the literature.
Background: Blomia tropicalis is an important mite species in the tropical and sub‐tropical regions of the world. Blo t 5 is the major allergen with up to 70% sensitization rates in B. tropicalis ...allergic populations.
Methods: Mice were immunized intramuscularly with naked plasmid DNA encoding Blo t 5 gene with in vivo electroporation. Blo t 5 monoclonal antibodies were generated using methylcellulose‐based hybridoma kit. Monoclonal antibody (mAb) 4A7 was characterized by two‐dimensional electrophoresis immunoblotting. A specific quantitative two‐site enzyme‐linked immunosorbent assay (ELISA) was developed with mAb 4A7 and guinea pigs Blo t 5 polyclonal antibody as capture and detection antibodies, respectively. This system was tested with Blo t 5 in crude extracts and dust samples.
Results: A high‐affinity mAb 4A7 recognizing several isoforms of Blo t 5 has been generated. Monoclonal antibody 4A7 is useful for immunoblotting and two‐site ELISA. The two‐site ELISA developed has a high sensitivity, with a detection limit of 10 pg/ml. The assay is species‐specific and recognized the same epitopes on both native and recombinant Blo t 5. The assay developed is able to detect Blo t 5 in commercial diagnostic and therapeutic B. tropicalis extract. Blo t 5 quantification in dust samples showed that Blo t 5 is present in a high quantity in Singapore dust.
Conclusions: A highly sensitive and specific two‐site ELISA has been developed. The assay system developed is useful for the quantification of Blo t 5 in mite and environmental dust extracts.
Background and ObjectivesThis study aimed to evaluate the efficacy and safety of transarterial chemoembolization (TACE) in patients with unresectable early or intermediate hepatocellular carcinoma ...(HCC) and Child-Pugh (CP)-B liver dysfunction. MethodsThis multicenter retrospective study enrolled patients with treatment-naïve HCC treated with TACE monotherapy between January 2012 and December 2020 at six Chinese hospitals. The primary outcome was overall survival (OS), and the secondary outcomes included the objective response rate (ORR) according to the modified RECIST and adverse events (AEs). Propensity score matching (PSM) was performed to reduce bias between the CP-B and CP-A groups. ResultsA total of 847 patients were included in the study. CP-A patients had significantly longer OS (median, 22.0 vs 19.3 months, P = 0.032) than CP-B (score of 7-9) patients, but a non-significant trend compared with CP-B (score of 7) patients (median, 22.0 vs 20.5 months, P = 0.254). After PSM, the median OS was 22.7 months for CP-A patients, while it was 19.3 months for CP-B (score of 7-9) patients (p = 0.026) and 20.5 months for CP-B (score of 7) patients (p = 0.155). CP-A patients achieved a significantly better ORR (53.0% vs 35.8%, P < 0.05) compared to CP-B (score of 7-9) patients, but a non-significant trend was observed in CP-B (score of 7) patients (53.0% vs 51.1%, P > 0.05). The post-embolization syndrome rates in the CP-A and CP-B (score of 7) cohorts were 52.1% and 53.3%, respectively. No new safety concerns were observed. ConclusionPatients with HCC with a CP score of 7 receiving TACE showed a similar prognosis and safety profile to CP-A patients.
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