A spectroscopic assay based on surface enhanced Raman scattering (SERS) using silver nanorod array substrates has been developed that allows for rapid detection of trace levels of viruses with a high ...degree of sensitivity and specificity. This novel SERS assay can detect spectral differences between viruses, viral strains, and viruses with gene deletions in biological media. The method provides rapid diagnostics for detection and characterization of viruses generating reproducible spectra without viral manipulation.
Gastrointestinal microbiota has significant impact on the nutrition and health of monogastric herbivores animals including donkey. However, so far the microbiota in different gastrointestinal ...compartments of healthy donkey has not been described. Therefore, we investigated the abundance and function of microbiota at different sites of the gastrointestinal tract (GIT) (foregut: stomach, duodenum, jejunum and ileum; hindgut: cecum, ventral colon, dorsal colon, and rectum) of healthy adult donkeys mainly based on 16S rRNA gene sequencing and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis. Collectively, our results showed that donkey has a rich, diverse and multi-functional microbiota along the GIT. In general, the richness and diversity of the microbiota are much higher in the hindgut relative to that in the foregut; at phylum level, the Firmicutes is dominant in the foregut while both Firmicutes and Bacteroides are abundant in the hindgut; at the genus level, Lactobacillus was dominant in the foregut while Streptococcus was more dominant in the hindgut. Our further PICRUSt analysis showed that varying microbiota along the GIT is functionally compatible with the corresponding physiological function of different GIT sites. For example, the microbes in the foregut are more active at carbohydrate metabolism, and in the hindgut are more active at amino acid metabolism. This work at the first time characterized the donkey digestive system from the aspects of microbial composition and function, provided an important basic data about donkey healthy gastrointestinal microbiota, which may be utilized to evaluate donkey health and also offer clues to further investigate donkey digestive system, nutrition, even to develop the microbial supplements.
Stochastic modeling is a useful approach for modeling fibrous materials that attempts to recreate fibrous materials’ structure using statistical data. However, several issues remain to be resolved in ...the stochastic modeling of fibrous materials—for example, estimating 3D fiber orientation distributions from 2D data, achieving the desired fiber tortuosity distributions, and dealing with fiber–fiber penetration. This work proposes innovative methods to (1) create a mapping from 2D fiber orientation data to 3D fiber orientation probability distributions, and vice versa; and (2) provide a means to select parameters de novo for random walks employing the popularized von Mises–Fisher distribution given that the desired tortuosity of the path is known. The proposed methods are incorporated alongside previously developed stochastic modeling techniques to simulate fiber network structures. First, fiber orientation distributions vary significantly depending on how a fibrous material is formed, and projection distortion affects the measurement of fiber orientation distributions when reported as 2D data such as histograms or polar plots. Relationships are developed to estimate 3D fiber orientation distributions from 2D data, accounting for projection distortion and the variety of orientation distributions observed in fibrous materials. We show that without correcting for projection distortion, fiber orientation distribution parameters could have errors of up to 100%. Second, in stochastic modeling, fiber tortuosity is usually treated with random walks, but no relationship is available for choosing random walk inputs to generate a desired fiber tortuosity. Relationships are also developed to relate the input parameters of von Mises–Fisher random walks to the expected tortuosity of the generated path—a necessary link to modeling fiber tortuosity distributions tractably and with empirical consistency. Using the developed relationships, we show that modeling of tortuous fibers from a distribution could be sped up by ~1200-fold and the uncertainty of selecting appropriate parameters could be eliminated. Third, randomly placing fibers in a simulation domain inevitably results in fiber–fiber penetration, and correcting this issue requires changes to the simulated fibrous material structure through non-penetration conditions. No thorough remedy can be offered here, but we statistically quantify the effects of enforcing non-penetration conditions on the fiber shape and orientation changes as well as the overall fibrous material model. This work offers tractable and transferable methods for treating fiber orientation and tortuosity that allow for empirical consistency in the stochastic modeling of fibrous materials.
Benefitting from lightweight, high strength, long life, and green recyclability, continuous fiber reinforced thermoplastic composite (CFTPC) pipes have attracted extensive interest, especially in the ...on-orbit additive manufacturing of structural components. However, the preparation of CFTPC pipes remains challenging due to the on-orbit limited space and high processing temperature of thermoplastic resin. Here, we report an effective approach for high performance carbon fiber/polyether-ether-ketone (CF/PEEK) thin-walled pipes via bidirectional reinforcement using the pultrusion-winding technique. The continuous fabrication of thin-walled pipes can be achieved, but the limitation by the size of core mold is also broken. The compressive and shear performance of CF/PEEK pipes with different layer designs have been studied based on experiments and simulations. With the increase in axial prepreg tape layer, the resultant CF/PEEK pipes exhibit greatly improved axial compression strength. The finite element analysis indicates that the maximum axial stress is decreased due to the axial enhancement. The flexural strength is greatly proved with pultrusion-winding cycles. The simulation confirms that the circumferential strain is effectively reduced. The high performance of bidirectional reinforced CF/PEEK pipes and the facile controllability of this approach highlight their suitability for utilization in on-orbit manufacturing of large-scale structures.
Twenty seven different bacteria isolates from 12 species were analyzed using intrinsic surface-enhanced Raman scattering (SERS) spectra with recently developed vancomycin coated silver nanorod (VAN ...AgNR) substrates. The VAN AgNR substrates could generate reproducible SERS spectra of the bacteria with little to no interference from the environment or bacterial by-products as compared to the pristine substrates. By taking advantage of the structural composition of the cellular wall which varies from species to species, the differentiation of bacterial species is demonstrated by using chemometric analyses on those spectra. A second chemometric analysis step within the species cluster is able to differentiate serotypes and strains. The spectral features used for serotype differentiation arises from the surface proteins, while Raman peaks from adenine dominate the differentiation of strains. In addition, due to the intrinsic structural differences in the cell walls, the SERS spectra can distinguish Gram-positive from Gram-negative bacteria with high sensitivity and specificity, as well as 100% accuracy on predicting test samples. Our results provide important insights for using SERS as a bacterial diagnostic tool and further guide the design of a SERS-based detection platform.
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•High quality and reproducible bacteria SERS spectra can be obtained on AgNR substrates.•Bacteria species can be differentiated by a first chemometric analysis.•Bacteria serotypes/strains can be differentiated by a second chemometric analysis.•Gram+ and Gram− bacteria can be distinguished with high sensitivity and specificity.
High-quality surface-enhanced Raman scattering (SERS) spectra of aflatoxin (AF) B(1), B(2), G(1) and G(2) have been acquired using silver nanorod (AgNR) array substrates fabricated by oblique angle ...deposition method. Significant vibrational peaks are identified on the argon plasma-cleaned substrates, and those peaks agree very well with the Raman spectra calculated by density function theory (DFT). The concentration-dependent SERS detection is also explored. The relationship between the concentration (C) of different AFs and the SERS intensity (I) of the Raman peak at Δν = 1592 cm(-1) is found to follow the general relationship I = AC(α), with α ranging from 0.32 to 0.46 for the four AFs. The limits of detection (LODs) reach 5 × 10(-5) mol L(-1) for AFB(1), 1 × 10(-4) mol L(-1) for AFB(2), and 5 × 10(-6) mol L(-1) for both AFG(1) and AFG(2) in bulk solution, or 6.17 × 10(-16) mol/1.93 × 10(-4) ng of AFB(1), 1.23 × 10(-15) mol/3.88 × 10(-4) ng for AFB(2), 6.17 × 10(-17) mol/2.03 × 10(-5) ng for AFG(1), and 6.17 × 10(-17) mol/2.04 × 10(-5) ng for AFG(2) per laser spot. Principal component analysis (PCA) is used to successfully differentiate these four different kinds of AFs at different concentrations up to their detection limits. The LODs obtained from PCA agree with the LODs obtained by using peak fitting method. With such a low detection limit and outstanding differentiation ability, we prove the possibility of utilizing the SERS detection system as a platform for highly sensitive mycotoxin detection.
Maximal information coefficient (MIC) is an indicator to explore the correlation between pairwise variables in large data sets, and the accuracy of MIC has an impact on the measure of dependence for ...each pair. To improve the equitability in an acceptable run-time, in this paper, an intelligent MIC (iMIC) is proposed for optimizing the partition on the y-axis to approximate the MIC with good accuracy. It is an iterative algorithm on quadratic optimization to generate a better characteristic matrix. During the process, the iMIC can quickly find out the local optimal value while using a lower number of iterations. It produces results that are close to the true MIC values by searching just n times, rather than n2 computations required for the previous method. In the compared experiments of 169 indexes about 202 countries from World Health Organization (WHO) data set, the proposed algorithm offers a better solution coupled with a reasonable run-time for MIC, and good performance search for the extreme values in fewer iterations. The iMIC develops the equitability keeping the satisfied accuracy with fast computational speed, potentially benefitting the relationship exploration in big data.
Hydrochemical research and identification of nitrate contamination are of great significant for the endorheic basin, and the Northern Huangqihai Basin (a typical endorheic basin) was comprehensively ...researched. The results showed that the main hydrochemical facies were HCO3–Mg·Ca and HCO3–Ca·Mg. Spatial variation coefficients of most indices were greater than 60%, which was probably caused by human activities. The hydrochemical evolution was mainly affected by rock weathering and also by cation exchange. The D–18O relationship of groundwater was δD = 5.93δ18O − 19.18, and the d–excess range was −1.60–+6.01‰, indicating that groundwater was mainly derived from precipitation and that contaminants were very likely to enter groundwater along with precipitation infiltration. The NO3(N) contents in groundwater exceeded the standard. Hydrochemical analyses indicated that precipitation, industrial activities and synthetic NO3 were unlikely to be the main sources of nitrate contamination in the study area. No obvious denitrification occurred in the transformation process of nitrate. The δ15N(NO3) values ranged from +0.29‰ to +14.39‰, and the δ18O(NO3) values ranged from −6.47‰ to +1.24‰. Based on the δ15N(NO3) – δ18O(NO3) dual isotope technique and hydrochemical methods, manure, sewage and NH4 fertilizers were identified to be the main sources of nitrate contamination. This study highlights the effectiveness of the integration of hydrochemical and isotopic data for nitrate source identification, and is significant for fully understanding groundwater hydrochemistry in endorheic basins and scientifically managing and protecting groundwater.
Herein, deep learning (DL) is used to predict the structural parameters of Ag nanohole arrays (NAs) for spectrum‐driving and color‐driving plasmonic applications. A dataset of transmission spectra ...and structural parameters of NAs is generated using finite‐difference time‐domain (FDTD) calculations and is converted to vivid structural colors using the corresponding transmission spectrum. A bidirectional neural network is used to train the transmission spectrum and structural color together. The accuracy of predicting the structural parameters using a desired spectrum is tested and found to be up to 0.99, with a determination coefficient of reproducing the desired spectrum and color to be 0.97 and 0.96, respectively. These values are higher compared to those when only training for spectrum, but requiring less training time. This strategy is able to inverse design the NAs in less than 1 s to maximize surface‐enhanced Raman scattering (SERS) enhancement by matching transmission resonance and laser excitation wavelength, and accurately regenerate colored images in 7.5 s, allowing for nanoscale printing at a resolution of approximately 100 000 dots in−1. This work has important implications for the efficient design of nanostructures for various plasmonic applications, such as plasmonic sensors, optical filters, metal‐enhanced fluorescence, SERS, and super‐resolution displays.
Deep learning is used to accurately predict the structural parameters of Ag nanohole arrays for plasmonic applications, achieving high accuracy in reproducing desired spectra and colors, and enabling nanoscale printing at ≈100 000 dpi. This efficient inverse design strategy offers significant implications for various plasmonic applications including sensors, optical filters, and super‐resolution displays.