•Immunological methods based on anti-TTR mAb were established.•Both BPF and BPS induced thyroid disruption in adult zebrafish.•TTR could be used as a biomarker to assess thyroid-disrupting effects in ...fish.•BPF may exhibit greater disruption effects than BPS at the same concentration.
The thyroid disrupting chemicals (TDCs) have raised great concerns due to their adverse impacts on thyroid hormones (THs). In this study, we investigated the thyroid-disrupting effects of bisphenol F (BPF) and bisphenol S (BPS), two major BPA substitutes, on adult zebrafish (Danio rerio). Firstly, anti-transthyretin (TTR) monoclonal antibody (anti-TTR mAb) was prepared and used to establish an indirect ELISA, which had a working range of 15.6∼1000 ng/mL of a detection limit of 6.1 ng/mL. The immunoassays based on anti-TTR mAb showed that exposure to BPF (10 and 100 μg/L) and BPS (100 μg/L) significantly elevated the levels of TTR protein in the plasma, liver, and brain tissues. Moreover, immunofluorescence showed that 100 μg/L BPF and BPS induced the production of TTR protein in liver and brain tissues. In addition, BPF and BPS increased THs levels and damaged thyroid tissue structure in adult female zebrafish. Especially, 100 μg/L BPF significantly increased T4 and T3 levels by 2.05 and 1.14 times, and induced pathological changes of thyroid follicles. The changes in the expression levels of genes involved in the hypothalamus-pituitary-thyroid (HPT) axis further illustrated that BPF and BPS had significant adverse effects on THs homeostasis and thyroid function in zebrafish. Therefore, TTR immunoassays could be used for the evaluation of thyroid-disrupting effects in fish and BPF exhibited greater disruption than BPS.
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•Prometryn significantly decreased the hatching rate of marine medaka embryos.•Prometryn exposure induced deformities and inhibited the growth of the larvae.•Prometryn increased the expression of ...heart-related genes in a dose-dependent manner.•Immunotoxic responses in newly hatched larvae were triggered by prometryn exposure.•The health risk of herbicide prometryn on marine fish should arouse great concern.
Triazine herbicides have been widely detected in marine environments because of their extensive usage in agriculture, but their impact on marine organisms is unclear. In this study, marine medaka (Oryzias melastigma) embryos were exposed to 0, 1, 10, 100, and 1000 μg/L prometryn, one of the most detected triazine herbicides, to investigate its potential effects. The results showed that 1, 10, 100, and 1000 µg/L prometryn not only induced yolk sac shrinkage and heart malformations, but also significantly delayed the hatching time and increased the heart rate and hatching failure rate of embryos. Moreover, 1, 10, 100, and 1000 μg/L prometryn caused obvious malformations and decreased the body length of the newly hatched larvae. After 21 d of exposure, increased larval death rate, decreased body length and width, and higher lipid accumulation were observed in the larvae from all prometryn groups. Furthermore, prometryn exposure upregulated the expression levels of cardiac development-related genes GATA, COX, ATPase, SmyD1, EPO, FGF8, NKX2, and BMP4 in the larvae. Transcriptome analysis revealed that 10 μg/L prometryn upregulated 604 genes, and the topmost pathways of differentially expressed genes were the complement and coagulation cascades and AMPK signaling pathways. qPCR results confirmed that prometryn exposure significantly increased the expression levels of the complement and coagulation cascade genes f2, f5, c3, and c5. This study demonstrated that environmentally relevant concentrations of prometryn induced significant toxicity in the early life stages of marine medaka. Therefore, the health risks of herbicides to marine organisms are of great concern.
This article dealt with the problem of dynamic-event-triggered fault detection (FD) for unmanned aerial vehicles (UAVs) in the <inline-formula> <tex-math notation="LaTeX">H _{i}/H _{\infty } ...</tex-math></inline-formula> optimization criterion. To make use of limited communication resources, a dynamic-event-triggered mechanism (ETM) was considered to determine whether system information will be transmitted to the ground station. Note that the transmitted data failing to satisfy a predefined triggered condition were discarded directly. As such, FD performance was affected by not only disturbances and faults but also dynamic-event-triggered transmission errors, namely, the errors between the data of non-event time and the current actual system. To solve this problem, a new dynamic-event-triggered <inline-formula> <tex-math notation="LaTeX">H _{i}/H _{\infty } </tex-math></inline-formula> optimization approach was presented, whose contribution lied in the complete decoupling of residuals and event transmission errors under dynamic ETM, which can avoid continuous communication and Zeno phenomenon. First, a dynamic ETM was presented to determine communication time. Then, a variable sampling period model of UAV was established based on the dynamic ETM. On this basis, a dynamic-event-triggered residual generator was constructed. In the <inline-formula> <tex-math notation="LaTeX">H _{i}/H _{\infty } </tex-math></inline-formula> optimization criterion, Riccati recursion was used to compute the optimum solution of a dynamic-event-triggered FD filter. Moreover, a new residual evaluation method was proposed, whose effectiveness and feasibility were finally verified by the altitude control system of a UAV.
MXene as a new member of 2D materials due to its metallic conductivity, rigid structure, hydrophilicity and functionalization has received extensive interests in the field of ion exchange membranes. ...Herein, different mass fractions of Ti3C2TX MXene were mixed into polyvinylidene fluoride (PVDF) by blending method to obtain cation exchange membranes (CEMs), and the electrodialysis (ED) properties of these membranes were characterized to determine the possibility of their application. With the addition of MXene, the anti-fouling ability of the membrane was effectively improved and the membrane resistance was reduced, and the overall performance reached the best when the content of MXene was 3%wt. The ED process results showed that the modified CEMs can reduce the energy consumption and improve the mass transfer capacity, and 3%wt MXene/PVDF CEM had better performance than other prepared CEMs. Furthermore, the improvement of mechanical and thermal stability of these membranes were attributed to the formation of strong interaction between PVDF and MXene. The preparation strategy of MXene/PVDF CEMs provides basic information for the rational design of separation membrane with high comprehensive performance and opens up a good prospect for the application of MXene in the field of electrodialysis.
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•Ti3C2TX MXene/PVDF cation exchange membranes for electrodialysis were prepared.•The addition of Ti3C2TX improves the hydrophilicity and pollution resistance of CEMs.•Mxene forms conductive networks in membranes to enhance the conductivity of CEMs.•The Ti3C2TX/PVDF CEMs have good mechanical stability and thermal stability.•The mass transfer performance of the modified CEMs in electrodialysis is improved.
Mastering the influence laws of parameters on the solution structure of nonlinear systems is the basis of carrying out vibration isolation and control.Many researches on solution structure and ...bifurcation phenomenon in parameter spaces are carried out broadly in many fields,and the research on nonlinear gear systems has attracted the attention of many scholars.But there is little study on the solution domain boundary of nonlinear gear systems.For a periodic non-autonomous nonlinear dynamic system with several control parameters,a solution domain boundary analysis method of nonlinear systems in parameter spaces is proposed,which combines the cell mapping method based on Poincaré point mapping in phase spaces with the domain decomposition technique of parameter spaces.The cell mapping is known as a global analysis method to analyze the global behavior of a nonlinear dynamic system with finite dimensions,and the basic idea of domain decomposition techniques is to divide and rule.The method is applied to analyze the solution domain boundaries in parameter spaces of a nonlinear gear system.The distribution of different period domains,chaos domain and the domain boundaries between different period domains and chaotic domain are obtained in control parameter spaces constituted by meshing damping ratio with excitation frequency,fluctuation coefficient of meshing stiffness and average exciting force respectively by calculation.The calculation results show that as the meshing damping increases,the responses of the system change towards a single motion,while the variations of the excitation frequency,meshing stiffness and exciting force make the solution domain presenting diversity.The proposed research contribution provides evidence for vibration control and parameter design of the gear system,and confirms the validity of the solution domain boundary analysis method.
This study investigates how positive emotion in tourism social media posts influences tourists' user engagement and its underlying mechanisms. Utilizing real-world data from Ctrip travel platform, ...our research adopts a multi-method approach encompassing archival analysis, survey, and experimental methods to provide robust evidence supporting the role of positive emotion in the tourism posts. Findings suggest a diminishing effect of positive emotion on user engagement. Drawing upon emotions as social information theory, we identify two contrasting mediating pathways: users' positive affect and perceived persuasive motive. Importantly, mediators exert differential effects at varying levels of emotional expression, collectively contributing to an inverted U-shaped relationship between emotional expression and user engagement. The conclusions offer strategic insights into the emotional expression tactics employed by self-media users when posting, while also serving as a reference for businesses aiming to enhance higher engagement levels among potential tourists.
•An asphalt-filler volume equivalent substitution method is proposed.•When stone volume is constant, filler is replaced with equal volume asphalt.•Influence of filler-asphalt ratio reduction is ...studied on skid resistance.•Influence of the proposed method on the road performance of mixtures is evaluated.•Mixture with excellent anti-rutting and Low-temperature deformation is designed.
The asphalt mixture designed with the Marshall mix design method has stable quality and convenient technical management, but the design range of the asphalt-aggregate ratio is small and difficult to adjust, and is therefore difficult to adapt to the design of functional pavement materials with different requirements. In order to solve these problems, the asphalt-filler volume equivalent substitution method (the volume equivalent substitution method) has been proposed. Based on the optimum asphalt-aggregate ratio of the Marshall method, the asphalt-aggregate ratio in the mixture is increased by the equal volume substitution of asphalt and filler. The increase in the asphalt volume is equal to the decrease in the filler volume, and vice versa. With the condition of constant aggregate gradation, the modified asphalt of asphalt mixtures with filler contents of 6.0%, 4.5%, 3.0%, 1.5%, and 0% is designed based on the AC-13 gradation. Based on this, a high-temperature stability test, a low-temperature crack resistance test, a four-point bending fatigue test, and other tests are carried out for the asphalt mixtures. The results show that the dynamic stability of each mix proportion designed with the volume equivalent substitution method is 4924 times/mm even under the most unfavorable conditions (the filler dose is 0%). The maximum bending tensile strain of the specimen with a 0% filler dosage can reach 9667 μ, which is more than twice that of the specimen with a 6% filler dosage. The fatigue test shows that increasing the asphalt content in the mixture is beneficial for improving fatigue durability. The research results show that the material designed by the volume equivalent substitution method has excellent road performance in each proportion. This paper provides a method for the flexible adjustment of the asphalt-aggregate ratio and the asphalt mixture comprehensive road performance. Using this method, the optimum asphalt-aggregate ratio needs to be determined comprehensively according to the stress characteristics and functional requirements of the pavement and the pavement performance index of the mixture, and further research is still needed.
The application of organic crystal in different photoelectric device has different demand on the crystal structure and morphology, while the self‐assembly of different materials into crystals with a ...desired morphology remains a challenge. Herein, using three binary halogen‐bonded cocrystals involving a nonplanar halogen accepter molecule tetra(4‐pyridyl)‐tetrathiafulvalene (TTF(py)4), co‐crystallized with halogen donor aryl iodides C6F6‐xIx (x = 2,3, 1,3,5‐trifluoro‐2,4,6‐triiodobenzene (IFB), meta‐1,3‐diiodotetrafluorobenzene (mdIFB) or para‐1,3‐diiodotetrafluorobenzene (pdIFB)), are reported. TTF(py)4‐IFB cocrystals are formed primarily via N···I halogen bonds and F···H hydrogen bonds, while TTF(py)4‐mdIFB cocrystals and TTF(py)4‐pdIFB cocrystals are formed primarily via π–π Interactions, N···I halogen bonds and F···H hydrogen bonds, homogeneous π–π interactions are dominant in TTF(py)4‐mdIFB, while heterogeneous π–π interactions are dominant in TTF(py)4‐pdIFB. Through predicted morphology based on the attachment energy (Eatt) theory, especially π–π interaction, the influence of intermolecular interactions on crystal morphology is analyzed. The study demonstrates that the π–π interaction plays an important role in regulating the crystal structure and morphology, the work provides a deeper understanding of π–π interactions in nonplanar binary halogen‐bonded cocrystals.
Using a nonplanar molecule as accepter and aryl iodidesas donors, three nonplanar cocrystals based on I···N halogen bonding are successfully fabricated. By changing the number and position of iodine in thecocrystals, the halogen bond, the π–π interactions between molecules are tuned, resulting the transformation of dimension and morphology of cocrystals.
Wound environment-responsive materials show great potential in skin repair. Herein, a temperature-responsive fibrous hydrogel was developed with the aid of the phase-change material of natural fatty ...acids. The extracellular matrix-like methacrylate gelatin (GelMA) nanofibrous hydrogels were fabricated via electrospinning and UV-crosslinking, followed by a depot of fatty acids/aspirin (ASP) encapsulated polydopamine (PDA). The as-prepared fibrous hydrogels showed a significantly faster ASP release at 40 °C compared with those of 25 °C and 37 °C, and an “on-off” drug release was achieved at heating and cooling cycles between 40 °C and 25 °C. The in vitro cell culture tests and in vivo wound healing study proved that GelMA-PDA-ASP nanofibrous hydrogels facilitated the cell growth and accelerated wound repair. Taken together, the thermosensitive drug-releasing nanofibrous hydrogel, showed a great potential for wound care.
•Green solvent ethanol aqueous was used to formulate GelMA nanofiber membranes by electrospinning technology.•The GelMA nanofibrous hydrogels combined the advantages of hydrogels and nanofibers.•GelMA-PDA-ASP nanofiber hydrogels presented temperature-responsive drug release.•GelMA-PDA-ASP nanofiber hydrogels promoted wound healing with a wound closure ratio of 93% on day 12.
Objective
Quantitative real-time PCR (qPCR) is used to detect the differential expression of circular RNAs in patients of premature ovarian insufficiency (POI), to explore the new biomarkers of POI ...that can be detected from blood as soon as possible.
Methods
The study collected plasma samples from 30 patients in POI group and 30 normal people group who meet the inclusion criteria, who visited the gynecology clinic of The First Affiliated Hospital of Guangzhou University of Chinese Medicine from July 2019 to December 2020. Then, circRNAs in plasma were extracted for qPCR validation.
Results
1. qPCR technology was performed on hsa_circRNA_008901 and hsa_circRNA_403959, and it was found that the levels of both were considerably downregulated in POI group. Clinical evaluation showed that both hsa_circRNA_008901 and hsa_circRNA_403959 have good diagnostic value for POI.
2. According to miRNA Regulatory Element (MRE) analysis, the predicted target miRNAs of hsa_circRNA_008901 are: hsa-miR-548c-3p, hsa-miR-924, hsa-miR-4677-5p, hsa-miR-6786-3p and hsa-miR-7974; the predicted target miRNAs of hsa_circRNA_403959 are: hsa-miR-1207-5p, hsa-miR-4691-5p, hsa-miR-4763-3p, hsa-miR-6807-5p and hsa-miR-7160-5p.
Conclusion
Compared with the normal group, the expression levels of hsa_circRNA_008901 and hsa_circRNA_403959 in the POI group were downregulated, suggesting that these two circRNAs may be potential biomarkers of POI. Bioinformatics analysis indicated that hsa_circRNA_008901 and hsa_circRNA_403959 may regulate their binding miRNA through the action form of “molecular sponge”, and then regulate the signaling pathway regulated by miRNA, and ultimately affect the disease progression of POI.
