Background Hepatocellular carcinoma (HCC) is one of the most lethal and malignant tumours worldwide. New therapeutic targets for HCC are urgently needed. CYCLOPS (copy number alterations yielding ...cancer liabilities owing to partial loss) genes have been noted to be associated with cancer-targeted therapies. Therefore, we intended to explore the effects of the CYCLOPS gene RBM17 on HCC oncogenesis to determine if it could be further used for targeted therapy. Methods We collected data on 12 types of cancer from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) queries for comparison with adjacent non-tumour tissues. RBM17 expression levels, clinicopathological factors and survival times were analysed. RNAseq data were downloaded from the Encyclopaedia of DNA Elements database for molecular mechanism exploration. Two representative HCC cell models were built to observe the proliferation capacity of HCC cells when RBM17 expression was inhibited by shRBM17. Cell cycle progression and apoptosis were also examined to investigate the pathogenesis of RBM17. Results Based on 6,136 clinical samples, RBM17 was markedly overexpressed in most cancers, especially HCC. Moreover, data from 442 patients revealed that high RBM17 expression levels were related to a worse prognosis. Overexpression of RBM17 was related to the iCluster1 molecular subgroup, TNM stage, and histologic grade. Pathway analysis of RNAseq data suggested that RBM17 was involved in mitosis. Further investigation revealed that the proliferation rates of HepG2 (P = 0.003) and SMMC-7721 (P = 0.030) cells were significantly reduced when RBM17 was knocked down. In addition, RBM17 knockdown also arrested the progression of the cell cycle, causing cells to halt at the G2/M phase. Increased apoptosis rates were also found in vitro. Conclusion These results suggest that RBM17 is a potential therapeutic target for HCC treatment.
Investigating the relationship between the glass-forming ability (GFA), mechanical properties, and structure of metallic glasses is crucial to understanding the nature of the metallic glass state. In ...this study, the correlation among the atomic structure, electronic valence band, and properties have been studied using Zr50Cu44.5−xAl5.5Agx (x = 0, 1.5, 3 at.%) bulk metallic glasses (BMGs). The results reveal that through the micro-addition of Ag, the GFA of Zr50Cu44.5Al5.5 BMG can be enhanced; meanwhile, the critical diameter of Zr50Cu44.5Al5.5 glass rods increases from approximately 2.5 mm to 5.0 mm with the addition of 3% Ag. Through the addition of Ag, the thermal stability of Zr50Cu44.5Al5.5 BMG is improved, and the proportion of icosahedral-like clusters increases. The plasticity of the Zr50Cu44.5−xAl5.5Agx (x = 0, 1.5, 3 at.%) BMGs decreased from 4.6% to 0.8% with the addition of Ag. The valence band spectrum of the Zr50Cu44.5−xAl5.5Agx (x = 0, 1.5, 3 at.%) BMGs indicates that with the addition of Ag, the p-d hybridization near the Fermi level is enhanced, and the binding energy will move to a lower value.
Ice, Cloud, and land Elevation Satellite-2 (ICESat-2) provides effective photon-counting light detection and ranging (LiDAR) data for estimating forest height across extensive geographical areas. ...Although prior studies have illustrated canopy conditions during leaf-on and leaf-off phases may influence ICESat-2 derived forest heights, a comprehensive understanding of this effect remains incomplete. This study seeks to comprehensively assess how varying canopy conditions (leaf-on/leaf-off) affect ICESat-2 forest height retrieval and modelling. First, the accuracies of ICESat-2 terrain and canopy heights under leaf-on and leaf-off conditions were validated. Second, random forest algorithm was utilized to model forest height by integrating ICESat-2, Sentinel-2, and other ancillary datasets. Finally, we evaluated the influence of leaf-on and leaf-off conditions on forest height retrieval and modelling. Results reveal higher consistency between ICESat-2 and airborne LiDAR-derived terrain heights compared to the agreement between two canopy height datasets. Accuracies of ICESat-2 terrain and canopy heights are higher under leaf-off conditions in contrast to leaf-on conditions. Notably, the accuracies of ICESat-2 terrain and canopy heights under various conditions are closely linked to canopy cover. Furthermore, the accuracy of forest height modelling can be enhanced by combining ICESat-2 data collected during both leaf-on and leaf-off seasons with further eliminating low-quality samples.
Traditional Chinese buildings serve as a carrier for the inheritance of traditional culture and national characteristics. In the context of rural revitalization, achieving the 3D reconstruction of ...traditional village buildings is a crucial technical approach to promoting rural planning, improving living environments, and establishing digital villages. However, traditional algorithms primarily target urban buildings, exhibiting limited adaptability and less ideal feature extraction performance for traditional residential buildings. As a result, guaranteeing the accuracy and reliability of 3D models for different types of traditional buildings remains challenging. In this paper, taking Jingping Village in Western Hunan as an example, we propose a method that combines multiple algorithms based on the slope segmentation of the roof to extract feature lines. Firstly, the VDVI and CSF algorithms are used to extract the building and roof point clouds based on the MVS point cloud. Secondly, according to roof features, village buildings are classified, and a 3D roof point cloud is projected into 2D regular grid data. Finally, the roof slope is segmented via slope direction, and internal and external feature lines are obtained after refinement through Canny edge detection and Hough straight line detection. The results indicate that the CSF algorithm can effectively extract the roofs of I-shaped, L-shaped, and U-shaped traditional buildings. The accuracy of roof surface segmentation based on slope exceeds 99.6%, which is significantly better than the RANSAC algorithm and the region segmentation algorithm. This method is capable of efficiently extracting the characteristic lines of roofs in low-rise buildings within traditional villages. It provides a reference method for achieving the high-precision modeling of traditional village architecture at a low cost and with high efficiency.
Mentha haplocalyx Briq (MHB) and its components have been proven to improve the growth performance of livestock and poultry. The aim of this experiment was to investigate the effects of MHB addition ...on growth performance, rumen and fecal microbiota, rumen fluid, serum and urine metabolism, and transcriptomics of rumen epithelial cells in meat sheep. Twelve Hu sheep were selected for the experiment and fed with basic diet (CON) and a basal diet supplemented with 80 g/kg DM of Mentha haplocalyx Briq (MHB). The experimental period was 10 weeks with the first 2 weeks as the pre-trial period. The results showed that compared with the CON group, the average daily weight gain of meat sheep in the MHB group increased by 20.1%; the total volatile fatty acid (VFA) concentration significantly increased (p < 0.05); The thickness of the cecal mucosal layer was significantly reduced (p < 0.01), while the thickness of the colonic mucosal layer was significantly increased (p < 0.05), the length of ileal villi significantly increased (p < 0.01), the thickness of colonic mucosal layer and rectal mucosal muscle layer significantly increased (p < 0.05), and the thickness of cecal mucosal layer significantly decreased (p < 0.05); The serum antioxidant capacity has increased. At the genus level, the addition of MHB changed the composition of rumen and fecal microbiota, increased the relative abundance of Paraprevotella, Alloprevotella, Marinilabilia, Saccharibacteria_genera_incertae_sedis, Subdivision5_genera_incertae_sedis and Ornatilinea in rumen microbiota, and decreased the relative abundance of Blautia (p < 0.05). The relative abundance of Prevotella, Clostridium XlVb and Parasutterella increased in fecal microbiota, while the relative abundance of Blautia and Coprococcus decreased (p < 0.05). There were significant differences in the concentrations of 105, 163, and 54 metabolites in the rumen, serum, and urine between the MHB group and the CON group (p < 0.05). The main metabolic pathways of the differences were pyrimidine metabolism, taurine and taurine metabolism, glyceride metabolism, and pentose phosphate pathway (p < 0.05), which had a significant impact on protein synthesis and energy metabolism. The transcriptome sequencing results showed that differentially expressed genes were mainly enriched in immune regulation, energy metabolism, and protein modification. Therefore, adding MHB improved the growth performance of lambs by altering rumen and intestinal microbiota, rumen, serum and urine metabolomics, and transcriptome.
Endometrial carcinoma (EC), a common female reproductive system malignant tumor, affects thousands of people with high morbidity and mortality worldwide. This study was aimed at developing a ...prediction model for the diagnosis of EC in the general population. First, we obtained datasets GSE63678, GSE106191, and GSE115810 from the Gene Expression Omnibus (GEO) database, dataset GSE17025 from the GEO database, and the RNA sequence of EC from The Cancer Genome Atlas (TCGA) database to constitute the training, test, and validation groups, respectively. Subsequently, the 96 most significantly differentially expressed genes (DEGs) were identified and analyzed for function and pathway enrichment in the training group. Next, we acquired the disease-specific genes by random forest and established an artificial neural network for the diagnosis. Receiver operating characteristic (ROC) curves were utilized to identify the signature across the three groups. Finally, immune infiltration was analyzed to reveal tumor-immune microenvironment (TIME) alterations in EC. The top 96 DEGs (77 down-regulated and 19 up-regulated genes) were primarily enriched in the interleukin-17 signaling pathway, protein digestion and absorption, and transcriptional misregulation in cancer. Subsequently, 14 characterizing genes of EC were identified by random forest. In the training, test, and validation groups, the artificial neural network was constructed with high diagnostic accuracies of 0.882, 0.864, and 0.839, respectively, and areas under the ROC curve (AUCs) of 0.928, 0.921, and 0.782, respectively. Finally, resting and activated mast cells were found to have increased in TIME. We constructed an artificial diagnostic model with excellent reliability for EC and uncovered variations in the immunological ecosystem of EC through integrated bioinformatics approaches, which might be potential diagnostic targets for EC.
Traditional residences are among the most important tangible cultural heritage. This paper evaluates and explores the quality of individual traditional residences and the heritage value of a complex ...of traditional residences in Western Hunan in China. The former indicates how well the external characteristics of the building are preserved, whilst the latter refers to the integration of use values, ecology principles and cultural features. Based on the survey of the selected 7 traditional villages, the authors have built a spatial database of these villages on the strength of GIS, RS and GPS techniques, and employed an architectural evaluation method to grade the exterior quality of individual traditional residences, followed by the construction of an evaluation indicator system and the use of entropy weight method to score the value of traditional residences, thereby systematically unveiling how indicators influence the value of traditional residences. The results reveal that well-preserved and prime-quality traditional residences are quite rare. Average-quality individual traditional residences outnumber other quality levels in all selected traditional villages. These villages differ in the value of their traditional residences, which is susceptible to both natural and cultural factors. Architectural elements play a dominant role, and the change in architectural form serves as an important criterion for determining whether the traditional residence in question has been transformed into a modern building. The value of traditional rural residences is mainly reflected in the authenticity of the architectural form and the building material, which are crucial to the intact pass-down of their unique architectural styles.
•Traditional residences were systematically investigated based on GIS, RS and GPS.•The quality of individual traditional rural residences was quantified and analyzed.•Well-preserved and prime-quality traditional residences are quite rare.•The entropy weight method was used to estimate the value of traditional residences.•The influencing factors of the value of traditional residences were unveiled.
This study evaluated the effects of Isatis Leaf (ISL) on the growth performance, gastrointestinal tissue morphology, rumen and intestinal microbiota, rumen, serum and urine metabolites, and rumen ...epithelial tissue transcriptome of fattening sheep.
Twelve 3.5-month-old healthy fattening sheep were randomly divided into two groups, each with 6 replicates, and fed with basal diet (CON) and basal diet supplemented with 80 g/kg ISL for 2.5 months. Gastrointestinal tract was collected for histological analysis, rumen fluid and feces were subjected to metagenomic analysis, rumen fluid, serum, and urine for metabolomics analysis, and rumen epithelial tissue for transcriptomics analysis.
The results showed that in the ISL group, the average daily gain and average daily feed intake of fattening sheep were significantly lower than those of the CON group (
< 0.05), and the rumen ammonia nitrogen level was significantly higher than that of the CON group (
< 0.01). The thickness of the reticulum and abomasum muscle layer was significantly increased (
< 0.05). At the genus level, the addition of ISL modified the composition of rumen and fecal microorganisms, and the relative abundance of Methanobrevibacter and Centipeda was significantly upregulated in rumen microorganisms, The relative abundance of Butyrivibrio, Saccharofermentans, Mogibacterium, and Pirellula was significantly downregulated (
< 0.05). In fecal microorganisms, the relative abundance of Papillibacter, Pseudoflavonifractor, Butyricicoccus, Anaerovorax, and Methanocorpusculum was significantly upregulated, while the relative abundance of Roseburia, Coprococcus, Clostridium XVIII, Butyrivibrio, Parasutterella, Macellibacteroides, and Porphyromonas was significantly downregulated (
< 0.05). There were 164, 107, and 77 different metabolites in the rumen, serum, and urine between the ISL and CON groups (
< 0.05). The differential metabolic pathways mainly included thiamine metabolism, niacin and nicotinamide metabolism, vitamin B6 metabolism, taurine and taurine metabolism, beta-Alanine metabolism and riboflavin metabolism. These metabolic pathways were mainly involved in the regulation of energy metabolism and immune function in fattening sheep. Transcriptome sequencing showed that differentially expressed genes were mainly enriched in cellular physiological processes, development, and immune regulation.
In summary, the addition of ISL to the diet had the effect of increasing rumen ammonia nitrogen levels, regulating gastrointestinal microbiota, promoting body fat metabolism, and enhancing immunity in fattening sheep.
Display omitted
•The grafted NH2-MCM-41 formed a uniform hydrophilic layer on the membrane surface.•The modified membrane was endowed with affinity to both Cr (VI) and Cu (II) ions.•Adsorption of ...both Cr (VI) and Cu (II) on membrane reached equilibrium at 5min.•The water containing trace Cr (VI) and Cu (II) could be purified during filtration.•Anti-fouling and separation performance of the modified membrane were improved.
A thin-film composite ultrafiltration (UF) membrane grafted with amine-functionalized MCM-41 was prepared and used for removing heavy metals from aqueous solution. The porous NH2-MCM-41 nano-particles formed a uniform hydrophilic and adsorptive layer on the thin-film which endowed the composite membrane with affinity to heavy metals and better anti-fouling performance. The adsorption capacity of Cr (VI) and Cu (II) on membrane were 2.8mg Cr/g and 3.7mg Cu/g, respectively, and their adsorption processes reached equilibrium around 5min. The continuous UF experiments demonstrated that the membrane can be used as an effective filter medium to purify the water containing trace heavy metals. The metals concentration in filtrates was maintained below initial concentration of 1mg/L (Cu) and 0.5mg/L (Cr) during the 7-hour filtration. The membrane’s adsorption capacity could be regenerated by being immersed in 0.5M HNO3. Besides, to examine its anti-fouling performance, the model NOM (natural organic matter) water was used as a feed solution. The composite membrane showed not only slower irreversible fouling evolution but also higher rejection of NOM compared with that without grafting NH2-MCM-41. The method reported in this study was efficient to enhance the rejection and anti-fouling performance of UF membrane, what’s more, endowed the membrane with affinity to heavy metals.
Display omitted
•Fe-Cu-MCM-41 showed excellent catalytic activity in DCF mineralization.•Bromate was inhibited by reducing direct oxidation of Br− and HBrO/BrO− by ozone.•Lewis sites and electron ...transfer favored for O3 decomposition and OH generation.•No oxalic acids were detected in DCF degradation by Fe-Cu-MCM-41/O3.
A flexible catalyst, Fe-Cu-MCM-41, was employed to enhance diclofenac (DCF) mineralization and inhibit bromate formation in catalytic ozonation process. Greater TOC removal was achieved in Fe-Cu-MCM-41/O3 process (78%) than those in Fe-MCM-41/O3 (65%), Cu-MCM-41/O3 (73%) and sole ozonation (42%). But it was interesting that both Cu-MCM-41/O3 and Fe-MCM-41/O3 achieved 93% bromate inhibition efficiency, only 71% inhibition efficiency was observed in Fe-Cu-MCM-41/O3. Influence of pH, TBA/NaHSO3 and detection of by-products were conducted to explore the mechanism. By Pyridine adsorption–IR and XPS, a relationship was found among activity of catalysts, Lewis acid sites and electron transfer effect between Fe (II/III) and Cu (I/II). Fe-Cu-MCM-41 promoted ozone decomposition to generate OH, which accounted for enhanced DCF mineralization. The consumption of aqueous O3 also suppressed the oxidative of Br− and HBrO/Br−. More HBrO/BrO− accumulated in catalytic ozonation process and less bromate generated. Bromate formation in Fe-Cu-MCM-41/O3 process was sensitive with pH value, the acidic condition was not favor for bromate formation. Both DCF mineralization and bromate inhibition were influenced by surface reaction. Moreover, Fe-Cu-MCM-41 showed excellent catalytic performance in suppressing the accumulation of carboxylic acid, especially for oxalic acid. Nearly no oxalic acid was detected during Fe-Cu-MCM-41/O3 process.
PDF
