•WH could continuously recognize of Cu2+ and EDTA in HEPES system.•The detection limits of WH for Cu2+ and EDTA were as low as 29.66 and 172.13 nmol·L−1, respectively.•WH had excellent AIE ...performance.•WH could be used for the detection of Cu2+ in alcoholic beverages.
A novel fluorescent probe, WH, was successfully designed and synthesized. WH demonstrated continuous detection capabilities for Cu2+ and EDTA in the HEPES system. Specifically, the introduction of copper ions resulted in fluorescence quenching, while the addition of EDTA led to the recovery of fluorescence. The detection limits of WH for Cu2+ and EDTA were calculated to be 29.66 and 172.13 nmol·L−1, respectively. Moreover, WH exhibited AIE property in THF/H2O system. According to the Job's plot, 1H NMR titration and mass spectrometry, WH could form a complex with Cu2+ in a 1:1 ratio, and the combinding constant was 8.2 × 103 L mol−1. WH exhibited outstanding selectivity, high sensitivity, large Stokes shift and strong anti-interference ability. Furthermore, WH had been successfully applied in the detection of Cu2+ in beer and red wine, showcasing its promising potential for practical application.
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Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) is a protein involved in the regulation of RNA processing, cell metabolism, migration, proliferation, and apoptosis. However, the effect of ...hnRNPA2/B1 on injured endothelial cells (ECs) remains unclear. We investigated the effect of hnRNPA2/B1 on lipopolysaccharide- (LPS-) induced vascular endothelial injury in human umbilical vein endothelial cells (HUVECs) and the underlying mechanisms. LPS was used to induce EC injury, and the roles of hnRNPA2/B1 in EC barrier dysfunction and inflammatory responses were measured by testing endothelial permeability and the expression of inflammatory factors after the suppression and overexpression of hnRNPA2/B1. To explore the underlying mechanism by which hnRNPA2/B1 regulates endothelial injury, we studied the VE- cadherin/beta-catenin pathway and NF-kappaB activation in HUVECs. The results showed that hnRNPA2/B1 was elevated in LPS- stimulated HUVECs. Moreover, knockdown of hnRNPA2/B1 aggravated endothelial injury by increasing EC permeability and promoting the secretion of the inflammatory cytokines TNF-alpha, IL-1beta, and IL-6. Overexpression of hnRNPA2/B1 can reduce the permeability and inflammatory response of HUVEC stimulated by LPS in vitro, while increasing the expression of VE-Cadherin and beta-catenin. Furthermore, the suppression of hnRNPA2/B1 increased the LPS-induced NF- kB activation and reduced the VE-cadherin/beta-catenin pathway. Taken together, these results suggest that hnRNPA2/B1 can regulate LPS-induced EC damage through regulating the NF-kappaB and VE-cadherin/beta-catenin pathways.
In this paper, the Cu2SnS3 hollow microspheres (CTS-HMSs) have been successfully synthesized via a hydrothermal route, in which ethylenediaminetetraacetic acid disodium salt (EDTA-Na2) is introduced ...as both a chelating agent and a template. X-ray diffraction (XRD) results indicate that EDTA-Na2 plays crucial roles in determining the phase of Cu2SnS3 products, and the morphological characterizations show that the shell thickness of hollow microspheres is about 0.2μm and the outer diameter is in the range of 1.0–1.2μm. In particular, charge–discharge performance and cycle performance for the novel CTS-HMS materials in lithium-ion batteries are preliminarily investigated, and the unique electrode material exhibits a high initial lithium ion storage capacity of 1316mAhg−1, suggesting a promising electrode material for lithium ion batteries.
•CTS-HMSs were synthesized by an EDTA-Na2-assisted hydrothermal method.•The microspheres display attractive hollow structures with porous feature on the surface.•The as-prepared CTS-HMSs exhibited a high initial storage capacity.
•Nisin and EDTA blended PBAT/TPS active packaging produced by blown-film extrusion.•Nisin improved compatibility of starch and PBAT films which increased smoothness.•EDTA increased hydrophilicity and ...oxygen barrier of PBAT/TPS films.•Active films with EDTA reduced microbial growth in chilled meat up to 1.4 log.•Nisin and EDTA stabilized redness and delayed lipid deterioration in packaged pork.
Biodegradable active packaging was produced by compounding nisin (3, 6 and 9%) and nisin-ethylenediaminetetraacetic acid (EDTA) (3 and 6%) mixtures with poly(butylene adipate terephthalate) and thermoplastic starch blends (PBAT/TPS) by blown-film extrusion. Nisin and EDTA interacted with polymers, involving CO stretching of ester bonds and increased compatibility. This plasticized the films and modified the crystallinity, surface roughness and thermal relaxation behavior. Barrier properties were improved due to modified hydrophilic-hydrophobic properties, compact structures and crystallites that restricted vapor and oxygen permeation. PBAT/TPS films containing EDTA and nisin effectively inhibited lipid degradation in pork tissues corresponding with stabilizing the CO ester bond of triacylglycerol. Microbial growth was also inhibited, particularly in EDTA-containing films up to 1.4 log. Inactivation of microorganisms stabilized redness and delayed meat discoloration, preserving the quality of packaged pork. Interaction between nisin, EDTA and polymers modified the morphology and film properties and functionalized biodegradable food packaging to inactivate microorganisms.
6-(Methylsulfinyl)hexyl isothiocyanate (6-MSITC), a derivative of glucosinolate with a six-carbon chain, is a compound found in wasabi and has diverse health-promoting properties. The biosynthesis of ...glucosinolates from methionine depends on a crucial step catalyzed methylthioalkylmalate synthases (MAMs), which are responsible for the generation of glucosinolates with varying chain lengths. In this study, our primary focus was the characterization of two methylthioalkyl malate synthases, MAM1-1 and MAM1-2, derived from Eutrema japonicum, commonly referred to as Japanese wasabi. Eutremajaponicum MAMs (EjMAMs) were expressed in an Escherichiacoli expression system, subsequently purified, and in vitro enzymatic activity was assayed. We explored the kinetic properties, optimal pH conditions, and cofactor preferences of EjMAMs and compared them with those of previously documented MAMs. Surprisingly, EjMAM1-2, categorized as a metallolyase family enzyme, displayed 20% of its maximum activity even in the absence of divalent metal cofactors or under high concentrations of EDTA. Additionally, we utilized AlphaFold2 to generate structural homology models of EjMAMs, and used in silico analysis and mutagenesis studies to investigate the key residues participating in catalytic activity. Moreover, we examined in vivo biosynthesis in E. coli containing Arabidopsis thaliana branched-chain amino acid transferase 3 (AtBCAT3) along with AtMAMs or EjMAMs and demonstrated that EjMAM1-2 exhibited the highest conversion rate among those MAMs, converting l-methionine to 2-(2-methylthio) ethyl malate (2-(2-MT)EM). EjMAM1-2 shows a unique property in vitro and highest activity on converting l-methionine to 2-(2-MT)EM in vivo which displays high potential for isothiocyanate biosynthesis in E. coli platform.
To understand the stability, chelation behaviour, and biological activity of 4-Formylpyridinethiosemicarbazone (H4FPT), it is important to recognize its interactive geometry. Hence, computational ...studies on geometrically optimized structures of thione and thiol forms of H4FPT were performed. Binary metal complexes of the ligand, H4FPT (L) with the Ni(II) and Cu(II) metal ions (M), were synthesized and characterized by various spectroanalytical techniques as elemental analysis, molar conductance, magnetic susceptibility measurements, LC-MS, TGA, IR, UV-Visible, ESR, and powder XRD. Elemental analysis, LC-MS, and TGA studies indicate 1:2 (ML2) composition for mononuclear Ni(II) complex and 1:1 (ML) composition for dinuclear Cu(II) complex. Electronic absorption titrations, fluorescence quenching studies, and viscosity measurements suggest intercalative mode of binding of the complexes with calf thymus DNA (CT-DNA). These complexes also promote hydrolytic cleavage of plasmid pBR322. The ligand (H4FPT) and its complexes showed moderate-to-good activity against Gram-positive and Gram-negative bacterial strains. The DPPH radical scavenging studies showed antioxidant nature of both complexes.
A unique combination of cotton fabric (CF) with a mixture of EDTA and APTES Fe3O4 magnetic particles was developed and utilized for the first time as an adsorbent for removing pollutants from ...wastewater. Initially, Fe3O4 was synthesized using the co-precipitation method. Further, the surface of Fe3O4 was modified by introducing amino functional groups through a reaction with APTES, resulting in Fe3O4-NH2. Following this, the surface of carbon fiber (CF) was altered using ethylenediaminetetraacetic acid (EDTA) to create CF@EDTA. Through the use of EDC-HCl and NHS, Fe3O4-NH2 was attached to the surface of CF@EDTA, resulting in the final product CF@EDTA/Fe3O4. Subsequently, the prepared CF@EDTA/Fe3O4 was utilized to adsorb metal pollutants from wastewater, with a thorough analysis conducted using various characterization techniques including FTIR, SEM, EDX, XRD, VSM, and XPS to study the materials. The study specifically aimed to assess the adsorption performance of our cotton-based material towards As(III) and Cr3+ metal ions. The pH study was also performed. Results indicated that the material exhibited an adsorption capacity of approximately 714 mg/g for As(III) ions and 708 mg/g for Cr3+ ions. The Langmuir and Freundlich models, as well as pseudo-first and second-order models were also analyzed. The Langmuir and pseudo-second-order models were found to best fit the data. In terms of regeneration and reusability, the materials showed straightforward regeneration and recyclability for up to 15 cycles. The remarkable adsorption capacity, combined with the unique blend of cotton and Fe3O4 magnet, along with its recyclability, positions our material CF@EDTA/Fe3O4 as a promising contender for wastewater treatment and other significant areas in water research.
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•An efficient type of cellulose cotton materials modified with Fe3O4 was synthesized.•Surface amination and grafting of Fe3O4 were performed successfully.•The Fe3O4 derived cellulose cotton based materials serve as adsorbent with promising new potential applications.•The modified absorbent material demonstrates enhanced adsorption ability and excellent regeneration ability.
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•Simultaneous Cu-EDTA decomplexation and Cr(Ⅵ) reduction were realized by plasma.•OH dominated Cu-EDTA decomplexation, leading to carboxylic acids production.•Reductive CO2− was ...in-situ generated by reactions of carboxylic acids and OH.•In-situ formation of reductive CO2− dominated Cr(Ⅵ) reduction.•Possible pathways of Cu-EDTA decomplexation and Cr(Ⅵ) reduction were proposed.
It is a great challenge to purify complex wastewater containing high toxic metal ions and heavy metal complexes. In this study, in-situ formation of reductive CO2− and its potential for Cr(VI) reduction were firstly investigated during Cu-EDTA decomposition in a discharge plasma system. Cr(Ⅵ) reduction efficiency and Cu-EDTA decomposition efficiency reached nearly 79% and 100% after 30 min treatment under optimum conditions, respectively, and further enhanced at acidic conditions. OH and 1O2 dominated Cu-EDTA decomposition by attacking chemical bonds in Cu-EDTA, such as Cu-O, C-O, and C-N, leading to the release of carboxylic acids. Reductive CO2− was in-situ generated by reactions between the carboxylic acids (formate and oxalic acid) and OH, and it dominated Cr(Ⅵ) reduction. Compared with single carboxyl byproducts, multiple carboxyl byproducts were more conducive to Cr(Ⅵ) reduction by forming more CO2−. Eaq− also participated in the Cr(Ⅵ) reduction process via electron transfer. Cr(III) and Cu(II) finally existed as Cr2O3, Cr(OH)3, copper based hydroxides and carbonates after CO2−/OH reactions. Possible pathways of simultaneous Cr(Ⅵ) reduction and Cu-EDTA decomposition were proposed finally. This study firstly proved endogenous CO2− formation in the discharge plasma system, which was able to treat complex waterbody, providing an alternative for oxidation-reduction removal of complex pollutants.
Li4Ti5O12 was synthesized by a modified and facile sol–gel method with ethylene diamine tetraacetic acid (EDTA) and citric acid (CA) as a bi-components chelating agent. The raw precursor and powders ...further calcined at various temperatures and holding time were characterized by XRD, FT-IR, TG, BET and SEM. Nano-scale Li4Ti5O12 oxides, with a high phase purity and good stoichiometry, can be obtained at a calcination temperature of 750 °C and higher. The Li4Ti5O12 nanoparticle shows a network morphology with high dispersion, which reached a capacity of 164 and 108 mAh g−1 at 1C and 10C discharge rate, respectively. The result of the cycling performance shows a high capacity maintenance ratio of 97% at 1C and 25 °C after 1000 cycles. Electrochemical lithium intercalation/extraction performance is also evaluated by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) at room temperature.
► An enhanced chelating agent scheme for uniformly distributed products. ► Ethylene diamine tetraacetic acid (EDTA) and citric acid (CA) as the bi-components chelating agent. ► The bi-components chelating agent to improve the particles dispersion and decrease the particle size. ► Nano-scale Li4Ti5O12 oxides show superior electrochemical performance and cycle life. ► The bi-components chelating agent scheme is robust and efficient.
Abstract
Objectives
Platelet aggregation in the presence of ethylenediaminetetraacetic acid (EDTA), is called EDTA-related pseudothrombocytopenia (EDTA-PTP), resulting in low platelet count by ...automatic cell counters.
Case presentation
Herein, we present a case of a 5-year-old female, who was referred to our laboratory due to persistent thrombocytopenia.
Conclusion
Our case report discusses the efficiency of the histograms and flag warnings of the cell counter, indicating the importance of these variables.
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