•Gaps in the producers’ knowledge of antibiotic use (e.g. compounds) was observed.•Screening tests revealed a 10 % of positive samples and 12.5 % close to the LOD.•Quinolones, sulfonamides and ...tetracyclines were detected in faeces of healthy sheep.•Tylosin was detected in raw milk (below the maximum residual limits) and whey.•No antibiotics were identified in fresh or ripened cheeses.
This study investigated antibiotic utilization in artisanal dairies and residue occurrence throughout the raw milk cheese production chain using commercial testing (Charm KIS and Eclipse Farm3G) and UHPLC-QqQ-MS/MS and LC-QqQ-MS/MS. The cross-sectional survey results revealed gaps in the producers’ knowledge of antibiotic use. Commercial testing detected antibiotic levels close to the LOD in 12.5 % of the samples, mainly in raw milk and whey, with 10.0 % testing positive, specifically in fresh and ripened cheeses, indicating that antibiotics are concentrated during cheese-making. Chromatographically, several antibiotics were identified in the faeces of healthy animals, with chlortetracycline (15.7 ± 34.5 µg/kg) and sulfamethazine (7.69 ± 16.5 µg/kg) predominating. However, only tylosin was identified in raw milk (3.28 ± 7.44 µg/kg) and whey (2.91 ± 6.55 µg/kg), and none were found in fresh or ripened cheeses. The discrepancy between commercial and analytical approaches is attributed to compounds or metabolites not covered chromatographically.
•The extracts from Eucalyptus globulus Labill leaves exhibited the activity against 2KSL.•69 ingredients were identified by GC–MS/MS and LC–MS/MS.•The molecular docking was used for predication of ...2KSL inhibitor.•The potential active compounds were verified by activity experiment.•Protocatechuic acid as a potential 2KSL inhibitor was found from Eucalyptus globulus Labill leaves.
Screening of active ingredients in plants is critical for the development of medicinal plants. As a traditional medicinal material, Eucalyptus globulus Labill leaves are regarded as a potential raw material that can be developed as a pesticide because of its original activity. This study proposes a molecular docking assisted UPLC-MS/MS, GC–MS/MS method for the identification and prediction of insecticidal ingredients in the extracts of Eucalyptus globulus leaves. The extracts of Eucalyptus globulus leaves were analyzed by UPLC-MS/MS, GC–MS/MS, 34 aqueous ingredients and 35 volatile ingredients were identified. The identified ingredients were docked with repellent receptor (2KSL). The results showed that protocatechuic acid had strong 2KSL inhibitory activity, which was presumed to be the main reason for the inhibition of 2KSL. Finally, the antifeedant experiment of protocatechuic acid on Henosepilachna vigintioctopunctata (Fabricius) was carried out. The results indicate that the method can effectively screen the active ingredients from the medicinal plants.
Coumarins are secondary plant metabolites which play a key role in plant–plant and plant–microbe interactions. In particular, they are highly involved in environmental stress responses. Coumarins can ...transfer from plants producing coumarins to non-coumarin-producing neighbouring plants. As in vitro studies have shown that coumarins are nematicidal, we hypothesized that this transfer may also result in enhanced resistance against plant parasitic nematodes in coumarin-receiving plants. To test if the uptake of coumarins in a non-coumarin- producing plant protects the plant against nematode attack, we incubated non-coumarin- producing pea seedlings in growth media with the coumarin umbelliferone for three weeks, after which the plants were transplanted into soil and inoculated with root-knot nematodes (Meloidogyne incognita). We quantified the coumarin content in pea organs and nematode root invasion 2, 4, and 6 weeks after transplantation. Umbelliferone was taken up by the pea roots and translocated to the shoots. As a result of metabolization, umbelliferone and its derived metabolites coumarin, scopoletin, and scopolin were detected in the plants. The root uptake of umbelliferone reduced root-knot nematode invasion significantly up to 4 weeks after the root exposure. Our results suggest that the root uptake and the transfer of bioactive compounds between plants can expand the understanding of plant–plant interactions.
•Pea plant roots absorbed umbelliferone and translocated it into shoots.•Pea plants metabolized umbelliferone to other coumarin metabolites.•Root uptake of umbelliferone suppressed root-knot nematode invasion.•The suppression effect lasted up to 4 weeks after the root exposure to umbelliferone.
In vitro simulated gastro-intestinal digestion of anthocyanins from mulberry (Morus atropurpurea Roxb.) was investigated in terms of bioaccessibility, antioxidant activity and the possible fate of ...anthocyanins in this study. Mulberry anthocyanins were isolated by aqueous two-phase system and purified by Sephadex LH-20 column separation, and Fraction 1 containing cyanidin-3-glycoside and cyanidin-3-rutinoside was obtained as the dominant fraction. The bioaccessibility of anthocyanins was greatly decreased after the intestinal digestion, the recoveries were only 0.34% (the IN sample) and 4.58% (the OUT sample), respectively. The radical scavenging ability showed that the digest have good antioxidant activity responsible for the phenolics generated from degradation of anthocyanins under intestinal environment. 18 phenolics were identified in the gastric–intestinal digest of Fraction 1 by HPLC–ESI-MS/MS, mainly small molecular flavonoids, phenolic acids and their derivatives. This study provided a scientific basis for further pharmacological activity of anthocyanins and their mechanisms of metabolic pathway research.
► In vitro simulated gastro-intestinal digestion of anthocyanins was studied. ► Bioaccessibility of anthocyanins after intestinal digestion was greatly decreased. ► Good antioxidant activity of the digest mainly attributed to the phenolics. ► 18 phenolics were identified in the gastric-intestinal digest of Fraction 1. ► Some phenolics might be inferred as the fate of anthocyanin decomposition.
•The new LC-MS/MS strategy detects polyphenol metabolites in edible flowers.•The pseudo-targeted screening method enables accuracy and quantification.•Using the new strategy, 43 polyphenol ...metabolites were detected in 27 edible flowers.•Significant variations in polyphenol types and levels among the 27 flowers.•Enriched research on polyphenols in edible flowers;model applicable to other species.
Edible flowers, as a food ingredient, are rich in bioactive substance and have received a lot of attention. In this study, we have established a pseudo-targeting method based on Multi Reaction Monitoring (MRM) - Enhanced Product Ions (EPI) in the Information Dependent Acquisition (IDA) mode. The results showed that a total of 43 metabolites, including flavonoids, phenolic acids, and terpenes were detected in 27 edible flowers using integrated liquid chromatography-tandem mass spectrometry (LC-MS/MS) strategy. Rutin was measured in all edible flowers, but the levels varied significantly among the different varieties of flowers, with the highest being about 3500 times the lowest. Albiflorin and paeoniflorin are polyphenolic substance unique to Paeonia suffruticosa. The cyanidin 3-O-glucoside chloride content in reddish-purple flowers is higher than that of other colors. Dicoumarin and 4-hydroxycoumarin were discovered in edible flowers for the first time. This study established a pseudo-targeted detection method to reveal the distribution of polyphenolic metabolites and enriched the research on polyphenolic metabolites in edible flowers. It can also serve as a general strategy to detect metabolites in other agricultural products, providing valuable information about potential metabolites for consumers.
Apatite is a ubiquitous accessory mineral in various rocks (e.g., igneous, metamorphic and clastic sedimentary rocks). However, precise and accurate U‐Pb LA‐ICP‐MS dating of apatite is often ...compromised by high common Pb levels. Among the different common Pb correction methods, the main advantage of the 204Pb correction method is that it does not assume U/radiogenic Pb concordance. However, 204Pb is difficult to measure using ICP‐MS instruments because of the isobaric interference of 204Hg on 204Pb. We overcome this limitation by using a reaction cell sandwiched between two quadrupoles within an ICP‐MS, which can allow the online chemical separation of two different elements. Ammonia reacts efficiently (> 98%) with Hg while isotopes of Pb are not affected. The approach was tested on eight apatite reference materials (McClure Mountain, NW‐1, UWA‐1, Otter Lake, Slyudyanka, MK‐1, Durango and Fish Canyon Tuff) for which there are independent constraints on the U‐Pb crystallisation age, by comparing U‐Pb dating results employing different reaction gas mixes (NH3‐N2O and NH3 only) in two laboratories. Based on the U‐Pb data and SEM analyses on each sample, we can exclude apatite inter‐ and intra‐grain U‐Pb age heterogeneity, except for a c. 4% variability of ages in the Otter Lake sample. Our results show that accuracy and precision for U‐Pb dating are not measurably affected by different reaction gases, and we accurately reproduce ages of numerous independently characterised apatites within 4% of the reference ages, and the age reproducibility is typically better than 2%.
Key Points
One reaction cell sandwiched between two quadrupoles can allow online chemical Pb‐Hg separation.
Ammonia reacts efficiently (> 98%) with Hg while isotopes of Pb are not affected.
Ages of numerous independently‐characterised apatites are reproduced accurately within 2% of the reference ages.
Abstract
Tall fescue (Schedonurus arundinaceus) is commonly grazed in the mid-south, but also has potential use for spring silage. During ensiling, protein can be converted to ammonia, but condensed ...tannins such as those in Quebracho (Schinopsis quebracho-colorado) can reduce this conversion. However, acid production may also be reduced. Quebracho tannin was added to chopped tall fescue and ensiled at different moisture concentrations to determine those effects on ammonia and silage fermentation products. Tall fescue was harvested with a Carter harvester at 1000 h, spread on concrete, mixed thoroughly, and dried for 1 and 2 h to achieve 67 and 44% moisture (high moisture (HM) and low moisture (LM), respectively). Quebracho tannin was either not applied, or was mixed with distilled water 48 h prior to packaging and applied at 1 and 2% of the total silage dry matter (DM) to both moisture treatments. Laboratory silos were pressed to achieve a packing density of 192 kg/m3 and stored at room temperature (23.7 ℃) for 60 d, after which sub-samples were removed and analyzed for fermentation parameters. Post-ensiling crude protein (CP) was greater (P < 0.05) from LM vs. HM, but did not differ among tannin treatments (P = 0.11). Ammonia concentrations (%CP equivalent) were greatest in 0% tannin (P < 0.05). A moisture by tannin interaction affected acetic acid, with greater concentrations in HM silages with 0 and 2% tannin compared to LM with 2% tannin. Lactic acid (%) was greater (P < 0.05) in 0 compared to 2% tannin, but 1% did not differ from either 0 or 2% tannin. Butyrate was greater (P < 0.05) in HM, and in silos with 2% tannin, but not 1% tannin. Overall, total acids (%) were greatest (P < 0.05) in HM but did not differ (P = 0.54) among tannin treatments. Therefore, ensiling tall fescue with tannins reduced proteolysis regardless of moisture concentration, and did not reduce fermentation up to 2% tannin inclusion.
Abstract
Little is understood about the relationships among environmental enrichment, temperament, productivity, and feeding behavior in cattle. The objective of this study was to examine the effect ...of divergent behavioral phenotypes for brush usage on temperament, productivity, and feeding behavior patterns in beef cattle. Four pens of Brahman cattle (n = 2 pens with 15 heifers/pen, and n = 2 pens with 19 steers/pen) were housed in dry lots each equipped with four electronic feedbunks (GrowSafe Systems). Animals were video recorded throughout an 85d commercial gain test. Video recordings were decoded on d 1, 2, 4, 8, 16, 32, and 64 using continuous observations for 15 minutes every 30 minutes from 08:00 to 20:00 to determine the total daily brush usage for focal animals that were selected based on d -19 exit velocities. Exit velocity and body weight were measured on d -19, 0, 1, 25, 50, and 70 and feeding behavior was monitored daily using the GrowSafe 4000E system. Animals were classified within gender into one of three phenotypes (high, medium, and low brush usage) based on ±0.5SD from the brush usage mean. A General Linear Mixed Model (PROC GLIMMIX) with brush use phenotype, sex and the interaction of these as fixed effects and pen as random effect was used for data analysis. For high and medium brush use phenotypes, steers had greater final BW than heifers (P = 0.0005), while heifer and steer final body weight did not differ when brush usage was low. High brush use steers had greater ADG (P = 0.0002) than high and medium brush use heifers, and low brush use steers and heifers. This same pattern was observed for DMI (P = 0.0003). Heifers were more temperamental than steers (P = 0.0008), and high and medium brush use heifers had faster exit velocities than low brush use steers. These results suggest that monitoring animal’s pleasurable behavior (brush use), rather than fear response alone, may be a useful behavioral indicator of cattle productivity.
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