In the context of the present work methods are developed, which allow the statistical estimation of feasible ranges of functional tyre characteristics for any combination of rim and tyre dimension ...under different operating conditions. Therefore, measurement data regarding energy efficiency, driving dynamics and comfort are statistically analyzed and interpreted with regard to their physical root causes.
Herein, a new phenomenon of enhanced electrochemiluminescence (ECL) emission by restricting intramolecular motion in the 2D ultra-thin Zr12-adb (adb = 9,10-anthracene dibenzoate) metal-organic ...framework (MOF) nanoplate was discovered for the first time. The coordination immobilization of adb in porous ultra-thin Zr12-adb nanoplate endowed the Zr12-adb excellent ECL performance, including stronger ECL signal and higher ECL efficiency relative to those of H2adb monomers and H2adb aggregates. In the 2D Zr12-adb nanoplate, the bridging ligand adb was stretched and fixed between two Zr12 clusters, which restricted intramolecular rotations and suppressed unnecessary energy loss caused by self-rotation, thereby remarkably improved the ECL intensity and efficiency. More importantly, the porous ultra-thin structure of Zr12-adb MOF nanoplate not only allowed the coreactants to diffuse into the MOF interior, making both internal and external adb be excited, but also shortened the migration distance of electrons, ions, coreactants and coreactant intermediates, which further improved the ECL efficiency of Zr12-adb and overcame the shortcoming of H2adb aggregates in which the internal luminophores were not easily excited. Regarding the excellent ECL properties above, Zr12-adb nanoplate was selected as a new ECL emitter incorporated with the bipedal walking molecular machine together to fabricate a biosensor for sensitive detection of mucin 1. The enhanced ECL by restriction of intramolecular motions in MOFs provided a new pathway to improve ECL intensity and efficiency, which lighted up a lamp for the design and manufacture of high-performance ECL materials based on MOFs, thus offering new opportunities to develop ultrasensitive ECL biosensors.
This work proposed a new and efficient strategy to enhance ECL for the first time, named RIM by MOFs for ECL enhancement, which not only opened up a new horizon for exploring novel strategies to enhance ECL, but also expanded the application of MOFs in bioassays and provided an unprecedented idea to devise outstanding ECL emitters. Display omitted
•A new and efficient strategy was proposed for the first time to enhance ECL, named RIM by MOFs for ECL enhancement.•Zr12-adb nanoplates was synthesized as a bright ECL emitter to construct highly sensitive and selective ECL biosensors.•The developed ECL biosensor realized ultrasensitive detection for MUC1.
Both the timing and kinetics of neurotransmitter release depend on the positioning of clustered Ca2+ channels in active zones to docked synaptic vesicles on presynaptic plasma membranes. However, how ...active zones form is not known. Here, we show that RIM and RIM-BP, via specific multivalent bindings, form dynamic and condensed assemblies through liquid-liquid phase separation. Voltage-gated Ca2+ channels (VGCCs), via C-terminal-tail-mediated direct binding to both RIM and RIM-BP, can be enriched to the RIM and RIM-BP condensates. We further show that RIM and RIM-BP, together with VGCCs, form dense clusters on the supported lipid membrane bilayers via phase separation. Therefore, RIMs and RIM-BPs are plausible organizers of active zones, and the formation of RIM and RIM-BP condensates may cluster VGCCs into nano- or microdomains and position the clustered Ca2+ channels with Ca2+ sensors on docked vesicles for efficient and precise synaptic transmissions.
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•RIM and RIM-BP mixture forms liquid-liquid phase-separation-mediated condensates•Specific multivalent interaction between RIM and RIM-BP is essential for the LLPS•RIM and RIM-BP condensates cluster Ca2+ channels in solution and on membrane surface•RIM and RIM-BP are plausible organizers of presynaptic active zones
Clustering of Ca2+ channels at presynaptic active zones is critical for precise control of neurotransmitter release. Wu et al. show that the presynaptic active zone scaffold proteins RIM and RIM-BP form self-assembled condensates via liquid-liquid phase separations capable of clustering voltage-gated Ca2+ channels on lipid membrane bilayers.
The impact of localized π-radicals on soot formation is explored by considering their electronic structure and computing their relative concentrations in flame conditions. Electronic structure ...calculations reveal that the presence of localized π-radicals on rim-based pentagonal rings is due to aromaticity. We further calculated a complete mechanism for the formation and elimination of the site from hydrogen additions and abstractions. A batch reactor with flame concentrations of H• and H2 was used to determine the time-dependent concentration of localized π-radicals. Low temperatures ( < 1000 K) favored the fully saturated rim-based pentagonal ring. Soot nucleation temperatures (1000–1500 K) give way to unsaturated rim-based pentagons being favored. Localized π-radicals on rim-based pentagonal rings are found to be in higher concentration than the aryl-type σ-radical on the rim-based pentagon (mole fractions of 10−6−10−7) in below < 1500 K, consistent with recent experimental observations. Higher temperatures favor the σ-radical and the concentration of the localized π-radical on rim-based pentagons becomes negligible. A kinetic Monte Carlo treatment of multiple sites indicates that multiple localized π-radicals are possible on a single molecule. These results reveal the importance of localized π-radicals on rim-based pentagonal rings for PAH chemistry leading to formation of soot nanoparticles in flames involving aromatic rim-linked hydrocarbons (ARLH).
•Modification of the balancer to the rim material condition measurement system.•Health of the rim: -new and good, -used and suitable for further use, -unusable.•Processing of vibration data using ANN ...as a rim material integrity classifier.•Comparison of AHC with SOM and MLP as classifiers for the examination of the rim.
Some vehicle damages can be related to the wheels' functional state, defined by rim and tire conditions. The technical condition of the rim is not simple to identify as it is related to the state of material structure. So far, little information has been presented about measurement systems and methods dedicated to rim technical condition identification that are acceptable by car service stations. Thus, the new measurement system and method are proposed in this paper. The identification process uses forced vibration spectra measurement and data processing with neural network use. For this purpose, the existing wheel balancer has been integrated with the computer coupled to a shaft actuator, vibration exciter, and accelerometer head. The developed software provides automation of measurement procedure and results classification, while the algorithm of data processing enables the proper detection of the three states of rim: new, used, and fit for further use, and not usable.
To evaluate how often tests of structure and function detect glaucoma progression at the same study visit. Tests include current glaucoma clinical tests and a new 3-dimensional (3D) optical coherence ...tomography (OCT) rim measurement.
Prospective cohort study METHODS: For 124 open-angle glaucoma patients at a single institution, one eye was randomly selected for each patient. Patients were included if they had open-angle glaucoma and if they had at least 4 yearly study visits. Study visits included a full dilated eye exam, disc photography (DP), Humphrey visual field (HVF 24-2) testing, 2D OCT retinal nerve fiber layer (RNFL) thickness measurements, and 3D OCT neuroretinal rim measurements (i.e., minimum distance band or MDB). For each test at each study visit, eyes were classified as progressors or non-progressors using event-based analysis. Agreement occurred if tests progressed in the same eye at the same study visit. Agreement between all compared tests were calculated as percentages of agreement.
The study included 124 open-angle glaucoma eyes, which had an average follow-up period of 66.9 + 16.4 months. Structural tests (i.e., DP, global RNFL thickness, and global MDB rim thickness) progressed at the same visit as the functional test (i.e., HVF testing) in only 5.0% 3/60 to 16.1% 13/81 of eyes. Global MDB thickness and global RNFL thickness showed similar agreement with functional HVF testing (i.e., 16.1% 13/81 and 8.3% 7/84 respectively), and global MDB thickness showed better structure-function agreement with HVF testing than between DP and HVF testing (i.e., 5.0% 3/60, P = 0.04). For all paired comparisons between testing methods, eyes with moderate glaucoma showed similar or better agreement than eyes with mild or severe glaucoma.
Clinical tests of structure and function do not usually progress at the same clinic visit. Most of the time, glaucoma progression is only detected by one or two tests.
The morphologic features of the multiple atrial septal defects assessed by TTE‐based 3D imaging were similar to those by 3D‐TEE. TTE‐based 3D model had excellent visibility, allowing observation of ...3D structure of the rims of the defects. It may be useful method for assessment of the multiple atrial septal defects.
Magmatic minerals record the pre-eruptive timescales of magma ascent and mixing in crustal reservoirs and conduits. Investigations of the mineral records of magmatic processes are fundamental to our ...understanding of what controls eruption style, as ascent rates and magma mixing processes are well known to control and/or trigger potentially hazardous explosive eruptions. Thus, amphibole reaction rims are often used to infer pre-eruptive magma dynamics, and in particular to estimate magma ascent rates. However, while several experimental studies have investigated amphibole destabilization during decompression, only two investigated thermal destabilization relevant to magma mixing processes. This study examines amphibole decomposition experimentally through isobaric heating of magnesio-hornblende phenocrysts within a natural high-silica andesite glass. The experiments first equilibrated for 24 h at 870 °C and 140 MPa at H2O-saturated conditions and ƒO2 ∼ Re–ReO prior to rapid heating to 880, 900, or 920 °C and hold times of 3–48 h. At 920 °C, rim thicknesses increased from 17 μm after 3 h, to 55 μm after 12 h, and became pseudomorphs after longer durations. At 900 °C, rim thicknesses increased from 7 μm after 3 h, to 80 μm after 24 h, to pseudomorphs after longer durations. At 880 °C, rim thicknesses increased from 7 μm after 3 h, to 18 μm after 36 h, to pseudomorphs after 48 h. Reaction rim microlites vary from 5–16 μm in size, with no systematic relationship between crystal size and the duration or magnitude of heating. Time-averaged rim microlite growth rates decrease steadily with increasing experimental duration (from 3.97×10−7 mms−1 to 3.1 to 3.5×10−8 mms−1). Time-averaged microlite nucleation rates also decrease with increasing experimental duration (from 1.2×103 mm−3s−1 to 5.3 mm−3 s−1). There is no systematic relationship between time-averaged growth or nucleation rates and the magnitude of the heating step. Ortho- and clinopyroxene together constitute 57–90 modal % mineralogy in each reaction rim. At constant temperature, clinopyroxene abundances decrease with increasing experimental duration, from 72 modal % (3 h at 900 °C) to 0% (48 h at 880 °C, and 36 h at 900 and 920 °C). Fe–Ti oxides increase from 6–12 modal % (after 3–6 h) to 26–34 modal % (after 36–48 h). Plagioclase occurs in relatively minor amounts (<1–11 modal %), with anorthite contents that increase from An56 to An88 from 3 to 36 h of heating. Distal glass compositions (>500 μm from reacted amphibole) are consistent with inter-microlite rim glasses (71.3–77.7 wt.% SiO2) within a given experiment and there is a weakly positive correlation between increasing run duration and inter-microlite melt SiO2 (68.9–78.5 wt.%). Our results indicate that experimental heating-induced amphibole reaction rims have thicknesses, textures, and mineralogies consistent with many of the natural reaction rims seen at arc-andesite volcanoes. They are also texturally consistent with experimental decompression reaction rims. On this basis it may be challenging to distinguish between decompression and heating mechanisms in nature.
•Amphibole reaction rims form rapidly (3–48 h) following 10–50 °C of heating.•Reaction rim growth rates increases with increasing temperature.•Rim microlites formed during heating are fine grained (<20 μm).•Rims microlites formed by heating include plagioclase, oxides, and pyroxene.•Unambiguous differentiation between heating and decompression rims is challenging.
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