•Development of hand knitted biocompatible composite meshes with dip coating.•Formation of SF mesh surface functionalized with polymer-natural extracts.•Meshes underwent physical characterizations, ...in vitro biological testing, in silico molecular docking, and in vivo analysis.•The composite meshes were non-cytotoxic, antimicrobial, and up-regulated gene expression.•PHBV-HE composite mesh facilitated soft tissue repair and regeneration.
Post-operative complications stemming from incompatible meshes often lead to delayed wound healing, seroma, infections, inappropriate tissue infiltration and pain. The present study was outlined to develop biocompatible composite hand-knitted silk meshes modified with polymers and natural extracts. Our study introduced hand-knitted B. mori silk fibroin as the primary mesh material, offering superior mechanical strength and biocompatibility. The spin-assisted dip coating achieved desirable morphology, internal structures, thickness, and surface roughness. Moreover, the application of biopolymeric composite coatings containing polymers and natural extracts introduced antimicrobial character, facilitated cell attachment, migration, proliferation, potentiating gene expression and accelerating the process of wound healing. These composite meshes are a viable solution for addressing post-op complications in hernia and soft tissue repair surgeries.
In this study, 9 silk-based composite meshes (modified with polymeric-extract blends through spin-assisted dip coating) were successfully developed. Experimental variants were then subjected to various characterizations including SEM, DMA and chemical analysis (FTIR and GC-MS). Modified meshes were evaluated for their physiological characteristics and biological responses (the basic criterion for the selection of composite silk mesh). The biological testing included (antimicrobial susceptibility testing, in vitro cell viability assay, cell attachment assay (NIH3T3 and hUc-MSCs), in vitro cell migration, in vitro gene expression analysis with NIH3T3, in silico molecular docking with bioactive ligands of HE extract and in vivo analysis with PHBV-HE and PHBV-Control composite meshes in rat models.
Results showed that all variants exhibited a multi-fiber morphology with significant surface coating, allowing for optimal drug release up to 72 h. This release facilitated antibacterial properties and biocompatibility, as evidenced by in vitro cell viability, migration assays and gene expression analysis. Among the variants, the PHBV-HE composite mesh demonstrated superior results. In the case of PHBV-coated polymeric controls, the SEM analysis concluded that the presence of coating reduced the pore size up to 39.62 % whereas, fiber diameter was increased by up to 19.89 % as compared to the control. The presence of a coating on the mesh improved the mechanical strength/modulus by 165.89 %, UTS by 185.38 % and reduced the % strain by 64.67 %. The fast release of HE from PHBV-HE composite mesh was 90.7% up to 72h, confirming that it can induce antibacterial activity against surgical infections.
PHBV-HE showed the highest cell viability, wound healing and gene expression. Based on appreciable biological evaluation results shown by PHBV-HE, in rat hernia models, only the PHBV-HE variant was tested for in-vivo analysis. Results confirmed its non-toxic nature and wound-healing abilities. Enhanced cell proliferation and wound healing observed both in vitro and in vivo indicated that PHBV-HE holds promise as a biomedical implant suggesting its potential for effective hernia and soft tissue repair and regeneration.
The quest for an ideal mesh solution for potentiating soft tissue repair and regeneration is a long road with infinite horizons. Hence, the application of natural biomaterials, polymers and natural extracts with specialized textile techniques can emerge as a futuristic approach to fabricating a versatile mesh design and composition. The present study was conducted to develop novel and functional surface functionalized antimicrobial and biocompatible knitted silk fibroin meshes for soft tissue and hernia repair by incorporating the principles of tissue engineering. Different polymer composites along with honey bee products were utilized to enhance morphological, biomechanical, antimicrobial and biocompatible characteristics to support soft tissue and hernia repair. Display omitted
Approximate composition, physico-chemical, techno-functional, amino acid profile, fatty acid profile, nutritional quality, antioxidant, anti-amylase and anti-lipase properties of bee bread, royal ...jelly, and bee propolis were investigated. The main differences between the analyzed bee products were found in the content of protein (royal jelly), polysaccharides (bee bread), balsam (propolis), wax (propolis), and polyphenol (propolis). Bee products had considerable oil-holding capacity, water-binding capacity, water-holding capacity, emulsifying activity, and foaming capacity. Bee products had higher amounts of glucogenic amino acid, non-essential amino acid, sweet amino acid, hydrophobic amino acid, essential amino acid, ketogenic amino acid, and flavor amino acids and lower amounts of bitter amino acid, non-protein amino acid, branched-chain amino acid, and aromatic amino acid. Bee products contained higher amounts of unsaturated fatty acid, polyunsaturated fatty acid, monounsaturated fatty acid, saturated fatty acid, omega-3, and omega-6, and lower amounts of omega-9 and omega-7. Bee products, especially royal jelly, had a considerable health-promoting index, hypocholesterolemic index, omega-6/omega-3, atherogenicity index, and thrombogenicity index. Bee bread, royal jelly, and bee propolis showed good radical scavenging activity and effectively inhibited amylase and lipase similar to the control. Propolis, bee bread, and royal jelly are proposed as possible techno-functional ingredients for food and health.
•The approximate composition and nutritional quality of bee products were investigated.•Physico-chemical and techno-functional properties of bee products were investigated.•Bee products revealed good lipid and protein nutritional quality.•Bee products revealed good physico-chemical and techno-functional properties.•Bee products revealed excellent antioxidant, anti-amylase, and anti-lipase capacities.
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•Stingless bee honey has high biological potential.•Stingless bee microbiota may promote natural fermentative process.•Stingless bee honey fermentation may promote deglycosylation of ...polyphenols.•Polyphenols extraction by acid hydrolysis may affect its original composition.
Stingless bees are native to tropical and subtropical countries, such as Brazil. The wide variety of species, the sources of food collection (nectar and pollen), and the climate conditions strongly affect the chemical composition of the honey, making this a unique product with peculiar characteristics. Stingless bee honey presents higher water content, higher acidity, and a lower sugar concentration when compared to Apis mellifera honey. Moreover, there is a wide variety of microorganisms in stingless bees’ environment, which leads their honey to go through a natural fermentative process during its production in the hive. Besides, fermentation and hydrolysis are effective ways to convert glycosides into aglycones, thus increasing the bioavailability of compounds. In this sense, stingless bee honey may possess a greater concentration of phenolic compounds aglycones than glycosides, which would increase its potential benefits. Therefore, this review aims to compile the most recent studies of stingless bee honey phenolic profile and its biological potential (antioxidant, antimicrobial, and anti-inflammatory activities) and a possible connection to its natural fermentation process.
•Red propolis was characterized by infrared-spectroscopy.•Infrared-spectroscopy achieved power comparable to the chromatography method.•Infrared-spectroscopy was used for the fast determination of ...phenolics in propolis.•The antioxidant activity of propolis was modeled by regression with spectroscopy.
Conventional methods for determining phenolics and their bioactive properties are expensive, time-consuming, and laborious. This hinders the quality control of red propolis, recognized for having different types of phenolic constituents with different bioactive properties, for example, its antioxidant properties. In this sense, we present a new application of near and medium infrared spectroscopy to predict phenolic constituents, total flavonoids, gallic acid, kaempferol, pyrocatechin, quercetin, and different antioxidant tests (DPPH radical, reducing power and protection of the β-carotene: linoleic acid system) of red propolis using chemometry. The optimized models showed good predictive capacity with a minimum correlation coefficient of 0.70, low associated error, and figures of merit that indicate the good predictive capacity in the validation of the models. These data show infrared spectroscopy as efficient to simultaneously predict nine quality analyzes of red propolis quickly and simply. This also avoids tedious procedures for traditional chromatographic and spectrophotometric tests.
Honey bee pollen is considered to be a food, and national pollen standards exist in different countries such as Brazil, Bulgaria, Poland and Switzerland. It is the aim of the present work to review ...pollen composition and the analytical methods used for the evaluation of high quality bee pollen. Based on the experience of different countries and on the results of published research, we propose quality criteria for bee pollen, hoping that in the future they will be used as world wide bee pollen standards.
•Bee pollens shows suitable physicochemical and nutritional values.•Bee pollen is a polyphenol-rich superfood with health-promoting benefits.•Pollen extracts have strong antioxidant and ...antihyperglycemic activity.•Bee pollen as a functional ingredient can improve the quality of food products.
Bee pollen is an imperative product for human use. Seven bee pollens were harvested from Morocco, and their chemical, biological and techno-functional properties were studied. All samples showed acceptable physicochemical and nutritional quality with a mean energy value of 239 kcal/100 g. FTIR spectra confirmed the presence of major constituents like carbohydrates, lipids, proteins and polyphenols. Moreover, pollens exhibited good techno-functional properties, like carbohydrate solubility (34.47–59.27 g/100 g), protein solubility (7.28–23.31 g/100 g), emulsifying stability (16.52–45.38 min), emulsifying activity (9.83–25.05 g/m3) water absorption capacity (1.06–2.19 g/g), oil absorption capacity (1.15–3.50 g/g) and water–oil absorption index (0.62–1.25). Bee pollen extracts revealed potent antioxidant capacity and digestive enzyme inhibitory activity associated with the presence of fifteen phenolic compounds belonging to flavons, flavonols, flavanones, flavan-3-ols, hydroxybenzoic and hydroxycinnamic acids, and stilbenes families. Present data indicate the possible application of bee pollen as a useful nutritional, bioactive and anti-foaming ingredient, replacing synthetic products in food industries.
Honey is a naturally sweet product of honeybees (Apis mellifera) made from the nectar of different plants, as well as honeydew. The average mineral content of honey with nectar origin is reported to ...be 0.1–0.2%, while that for honeydew is 1%. Mineral concentration in honey was found to be correlated with geographical and botanical origins, geochemical soil composition and local pollution. Thus, honeys are considered to be excellent indicators of the environmental factors.
In this study, 187 samples were collected from all over Hungary, including 11 different types of honey and special bee products. The country was divided into 7 regions based on the NUTS system and the samples were grouped accordingly. The concentration of 4 macro elements (Na, Ca, Mg, K) and 15 micro elements (Al, B, Ba, Bi, Cd, Co, Cr, Cu, Fe, Li, Mn, Ni, Pb, Sr, Zn) were determined by the relatively new MP-AES technique. Experimental data were evaluated by statistical analysis.
The results of Canonical Discriminant Analysis (CDA) indicated that considering the geological origin, Central Hungary and Western Transdanubia showed some separation from the other sampling regions as well as the differentiation of five honey types was also observed among the collected species. Higher difference was found between the species than the regions indicating that the botanical aspects have a higher effect compared to the geological ones on the elemental composition of the studied honey samples. From the measured elements potassium was present in the highest concentration and chestnut honeys were observed to have outstanding overall mineral level. It can be further concluded that the MP-AES method is proved to be a suitable and cost-effective alternative for the elemental analysis of prepared honey samples.
•The effect of botanical and geographical aspects on the mineral content of honey was investigated•Botanical origin has a higher effect on the elemental concentration•The outstanding overall mineral content of chestnut honey is observed•MP-AES method is proved to be a cost-effective alternative of ICP-OES in routine analysis of honey
Bee Products in Dermatology and Skin Care Kurek-Górecka, Anna; Górecki, Michał; Rzepecka-Stojko, Anna ...
Molecules (Basel, Switzerland),
01/2020, Volume:
25, Issue:
3
Journal Article
Peer reviewed
Open access
Honey, propolis, bee pollen, bee bread, royal jelly, beeswax and bee venom are natural products which have been used in medicine since ancient times. Nowadays, studies indicate that natural bee ...products can be used for skin treatment and care. Biological properties of these products are related to flavonoids they contain like: chrysin, apigenin, kaempferol, quercetin, galangin, pinocembrin or naringenin. Several pharmacological activities of phenolic acids and flavonoids, and also 10-hydroxy-
-2-decenoic acid, which is present in royal jelly, have been reported. Royal jelly has multitude of pharmacological activities: antibiotic, antiinflammatory, antiallergenic, tonic and antiaging. Honey, propolis and pollen are used to heal burn wounds, and they possess numerous functional properties such as: antibacterial, anti-inflammatory, antioxidant, disinfectant, antifungal and antiviral. Beeswax is used for production of cosmetics and ointments in pharmacy. Due to a large number of biological activities, bee products could be considered as important ingredients in medicines and cosmetics applied to skin.
The American stingless bees species have been used in management and breeding practices by local civilizations since the pre-Columbian era. Currently, many of these species are managed commercially ...and maintained in meliponaries. However, divergences exist among authors about the ecological utility of these practices. Some argue that meliponaries could serve to maintain local biodiversity while others argue that they have the opposite effect. Due to pressure from beekeepers and environmentalists there are efforts to draft specific rules that legislate production and market focusing on conservating native bees. In recent years, these norms have become more specific due to the use of empirical data from the scientific community and demands from social groups and producers. This paper presents a revision on Brazilian legislation as well its applicabilities and proposes alterations in the Environmental Crimes Law.
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