Bisphenol F (BPF) and bisphenol S (BPS) are increasingly used as substitutes for bisphenol A (BPA), an endocrine disrupting chemical (EDC) with obesogenic activity. We investigated the in vitro ...effects of BPS and BPF on the adipogenesis of human adipose-derived stem cells (hASCs) exposed to different doses (0.01, 0.1, 1, 10 and 25 μM), stopping the adipogenic process at 7 or 14 days. Intracellular lipid accumulation was quantified by the Oil Red O assay, gene expression of peroxisome proliferator-activated receptor gamma (PPARγ), CCAT/enhancer-binding protein (C/EBPα), lipoprotein-lipase (LPL) and fatty acid binding protein 4 (FABP4), by quantitative real-time polymerase chain reaction (qRT-PCR) and protein levels by Western Blot. hASCs with BPF or BPS produced a linear dose-response increase in intracellular lipid accumulation and in gene expression of the adipogenic markers, confirmed by protein levels. Co-treatment ICI 182,780 significantly inhibited BPF- but not BPS-induced lipid accumulation. Given the affinity of bisphenols for diverse nuclear receptors, their obesogenic effects may result from a combination of pathways rather than a single mechanism. Further research is warranted on the manner in which chemicals interfere with adipogenic differentiation. To our best knowledge, this report shows for the first time the obesogenic potential of BPF in hASCs.
•BPF and BPS promote intracellular lipid accumulation in hASCs.•Expression of key adipogenic genes increases in response to BPF and BPS.•Our findings suggest that BPF and BPS are not safe alternatives to BPA.•The obesogenic potential of BPF in human adipogenesis is reported for the first time.
To address the issue of bisphenol A (BPA) contamination in wastewater, a novel hydrogel, sodium alginate/cellulose nanofibrils/ZIF-8 composite hydrogel (SCZC), was synthesized for efficient BPA ...removal. The SCZC exhibited an exceptional adsorption capacity of 1696 mg/g, aligning well with both Langmuir and pseudo-second-order models. Furthermore, it exhibited remarkable regeneration properties, maintaining 89.1 % of its adsorption capacity even after undergoing five adsorption-desorption cycles. The synthesized SCZC also acted as a fluorescent sensor for detecting BPA, employing dynamic quenching and offering linear detection ranges of 10–100 mg/L and 0.2–1.0 μg/L, with a low detection limit of 0.06 μg/L. Analysis of adsorption and detection mechanisms revealed that SCZC's exceptional performance could be attributed to the three-dimensional (3D) porous structure formed by sodium alginate and cellulose nanofibrils. Economic analysis indicated that SCZC, in comparison to commercially activated carbon, was relatively inexpensive. This study introduces a novel approach for designing and preparing a sodium alginate-based hydrogel incorporating metal-organic frameworks, offering simultaneous BPA detection and removal capabilities.
Display omitted
•Sodium alginate/cellulose nanofibrils/ZIF-8 composite hydrogel (SCZC) was prepared.•SCZC simultaneously has efficient adsorption and sensitive detection abilities.•Maximum adsorption capacity of the SCZC for BPA was 1696 mg/g.•SCZC had sensitive detection ability with low detection limit of 0.06 μg/L.
Bisphenol A (BPA) is a well-known endocrine disrupter used in several consumer products. Restricted use of BPA has led to increased use of bisphenol F (BPF) and bisphenol S (BPS). While previous ...studies found no associations between prenatal BPA and BPF exposure and bone mineral density (BMD), two recent cohort studies found that prenatal BPS exposure was negatively associated with bone mineral density in the offspring.
To determine possible associations between maternal and child urinary bisphenol concentrations, BMD and bone mineral content (BMC) in 7-year-old healthy children.
Pregnant women were recruited in 2010–2012 to participate in the Odense Child Cohort (OCC), Denmark. Maternal urine samples were collected in gestational week 28 and urinary BPA concentration was measured by isotope diluted LC-MS/MS. The children delivered a urine sample at age 7 years in which BPA, BPF and BPS were measured by an extended LS-MS/MS method based on the original method. At age 7 years DXA scans were performed and BMC and Z-score for BMD calculated. Associations between osmolality adjusted urinary maternal BPA and child BPA, BPF and BPS concentrations and BMC and BMD Z-score were examined by multiple linear regression analysis adjusted for potential confounders. Additionally, a combined effect of the bisphenols were evaluated by including the sum of child urinary BPA, BPF and BPS concentrations in the statistical analyses.
A total of 546 mothers and 453 children aged 7 years participated. BPA was detected in 84% and 96% of the maternal and child urine samples, respectively. We found no significant association between maternal urinary BPA concentration during pregnancy and BMC and BMD Z-score in 7-year-old children. In addition, no association between current bisphenol exposure in tertiles and bone density was found, interestingly, current BPA and summed bisphenol exposure in the highest 10% was associated with lower BMD Z-score at age 7-years, statistically significant for boys.
In these low exposed children we found no association between prenatal or current bisphenol exposure in tertiles and BMD in healthy children, however, the highest 10% exposed children had lower BMD, significant for boys, suggesting a negative impact with high bisphenol exposure. The short half-lives of bisphenols and the cross-sectional nature of the child exposure prompt more longitudinal studies to further clarify this topic.
As a substitute of bisphenol A (BPA), bisphenol S (BPS) has been applied in consumer products present in our daily lives. With a similar chemical structure as BPA, BPS has also been demonstrated as ...an exogenous endocrine disrupting chemical. Compared with a large number of studies on BPA, investigation on BPS has remained limited. In this study, we reviewed the literature of BPS mainly published during 2010–2017, including its environmental distributions, toxicities, and human exposure. The data demonstrated that BPS is now ubiquitous in the environment and found worldwide, but generally with concentration levels lower than BPA in various environment media, including water, sediment, sludge, indoor dust and air, consumer products, and human urine. However, we found that the concentration levels of BPS in aquatic environments, especially water samples, were almost comparable or equal to that of BPA. Our summary also indicated that process speed of substituting BPA with BPS in consumer products in the U.S. was relatively faster than other countries. In addition, we summarized the toxicities of exposure to BPS both in vivo and in vitro experiments. The current data supports that exposure to BPS may have adverse effects on reproductive systems, endocrine systems, and nervous systems in animals and humans, and may trigger oxidative stress. The occurrence of BPS was frequently reported in human urine, but rarely in other human samples. The current research indicates that food is the dominant source for human exposure to BPS, and the contribution of personal care product usage is low. The occurrence of BPS and their metabolites in the human body and the guidelines for BPS exposure merit further investigation.
Display omitted
•BPA was gradually substituted by BPS in consumer products worldwide.•The concentration ratios of BPS to BPA were higher in the aquatic environment than in other environmental media.•Food is the dominant source for general population exposure to BPS.
Bisphenol A (BPA) is a widely produced chemical that is mainly used as raw material for manufacturing plastic products. It is an endocrine disruptor and causes irreversible damage to the human body. ...Bisphenol S (BPS), an alternative to BPA, has low dose effects on toxicology and genotoxicity. Herein, we constructed a highly porous crystalline covalent organic framework (COF, CTpPa-2)-modified glassy carbon electrode (GCE) for the electrochemical sensing of BPA and BPS. The electrochemical properties of the CTpPa-2/GCE were characterized using galvanostatic charge-discharge, cyclic voltammetry and differential pulse voltammetry. The CTpPa-2/GCE exhibited remarkable electrocatalytic activity, and the electrochemical responses for BPA and BPS were found to be linear in the concentration ranges of 0.1–50 μM and 0.5–50 μM with detection limits of 0.02 μM and 0.09 μM (S/N = 3), respectively. Moreover, the fabricated sensor was utilized to determine BPA and BPS in bottle samples with recoveries of 87.0%–92.2% and migration rates of 13.2%–28.0%.
An electrochemical sensing platform based on COF CTpPa-2 has been developed for the determination of BPA and BPS in bottle samples and for the determination of their migration rates. Display omitted
•The covalent organic framework improves the electrocatalytic performance.•An electrochemical sensor for bisphenol A and bisphenol S was developed.•The sensor has potential applications in environmental analysis and food safety.
An electrochemical sensing platform based on COF CTpPa-2 has been constructed for the determination of BPA and BPS in bottle samples and for the determination of their migration rates.
Endocrine disruptors such as bisphenol A (BPA) and some of its analogues, including BPS, BPAF, and BPE, are used extensively in the manufacture of plastics. These synthetic chemicals could seriously ...alter the functionality of the female reproductive system. Although the number of studies conducted on other types of bisphenols is smaller than the number of studies on BPA, the purpose of this review study was to evaluate the effects of bisphenol compounds, particularly BPA, on hormone production and on genes involved in ovarian steroidogenesis in both in vitro (human and animal cell lines) and in vivo (animal models) studies.
The current data show that exposure to bisphenol compounds has adverse effects on ovarian steroidogenesis. For example, BPA, BPS, and BPAF can alter the normal function of the hypothalamic-pituitary-gonadal (HPG) axis by targeting kisspeptin neurons involved in steroid feedback signals to gonadotropin-releasing hormone (GnRH) cells, resulting in abnormal production of LH and FSH. Exposure to BPA, BPS, BPF, and BPB had adverse effects on the release of some hormones, namely 17-β-estradiol (E2), progesterone (P4), and testosterone (T). BPA, BPE, BPS, BPF, and BPAF are also capable of negatively altering the transcription of a number of genes involved in ovarian steroidogenesis, such as the steroidogenic acute regulatory protein (StAR, involved in the transfer of cholesterol from the outer to the inner mitochondrial membrane, where the steroidogenesis process begins), cytochrome P450 family 17 subfamily A member 1 (Cyp17a1, which is involved in the biosynthesis of androgens such as testosterone), 3 beta-hydroxysteroid dehydrogenase enzyme (3β-HSD, involved in the biosynthesis of P4), and cytochrome P450 family 19 subfamily A member 1 (Cyp19a1, involved in the biosynthesis of E2). Exposure to BPA, BPB, BPF, and BPS at prenatal or prepubertal stages could decrease the number of antral follicles by activating apoptosis and autophagy pathways, resulting in decreased production of E2 and P4 by granulosa cells (GCs) and theca cells (TCs), respectively. BPA and BPS impair ovarian steroidogenesis by reducing the function of some important cell receptors such as estrogens (ERs, including ERα and ERβ), progesterone (PgR), the orphan estrogen receptor gamma (ERRγ), the androgen receptor (AR), the G protein-coupled estrogen receptor (GPER), the FSHR (follicle-stimulating hormone receptor), and the LHCGR (luteinizing hormone/choriogonadotropin receptor). In animal models, the effects of bisphenol compounds depend on the type of animals, their age, and the duration and dose of bisphenols, while in cell line studies the duration and doses of bisphenols are the matter.
Bisphenol A (BPA) is a well-known endocrine-disrupting chemical with estrogenic activity. The widespread exposure of individuals to BPA is suspected to affect a variety of physiological functions, ...including reproduction, development, and metabolism. Here we report the mechanisms by which BPA and three of its analogues bisphenol B (BPB), bisphenol F (BPF), and bisphenol S (BPS) cause generation of reactive oxygen species (ROS), sperm DNA damage, and oxidative stress in both in vivo and in vitro rat models. Sperm were incubated with different concentrations (1, 10, and 100 µg/L) of BPA and its analogues BPB, BPF, and BPS for 2 h. BPA and its analogues were observed to increase DNA fragmentation, formation of ROS, and affected levels of superoxide dismutase at higher concentration groups. In an in vivo experiment, rats were exposed to different concentrations (5, 25, and 50 mg/kg/day) of BPA, BPB, BPF, and BPS for 28 days. In the higher dose (50 mg/kg/day) treated groups of BPA and its analogues BPB, BPF, and BPS, DNA damage was observed while the motility of sperm was not affected.
Bisphenol analogues are prevalent globally because of rampant usage and imprecise processing techniques, prompting alerts about environmental and health hazards. The method employed in this study by ...solid phase extraction (SPE) and liquid chromatography-tandem quadrupole mass spectrometer (LC-MS/MS) for both quantification and qualitative analysis of the bisphenol compounds in the surface water samples. The coastal and estuarine surface water of Port Dickson and Lukut ranges from 1.32 ng/L to 1890.51 ng/L of bisphenol analogues. BPF mean concentration at 1143.88 ng/L is the highest, followed by BPA and BPS at 59.01 ng/L and 10.96 ng/L, respectively. Based on RQm for bisphenol analogues, the highest for BPF at 2.49 (RQ > 1, high risk), followed by BPS at 0.12 (0.1 < RQ < 1, medium risk) and BPA at 0.09 (0.1 < RQ < 1, medium risk). The presence and current risk of bisphenols analogues should alert the possible water quality degradation soon.
•Bisphenols analogues present in marine ecosystems.•BPF mean concentration at 1143.88 ng/L is the highest.•BPA is the highest frequency detected in marine ecosystems.•BPF is the most ecologically high risk compared to BPA and BPS.
PDF
