Linguistic labels (e.g., “chair”) seem to activate visual properties of the objects to which they refer. Here we investigated whether language-based activation of visual representations can affect ...the ability to simply detect the presence of an object. We used continuous flash suppression to suppress visual awareness of familiar objects while they were continuously presented to one eye. Participants made simple detection decisions, indicating whether they saw any image. Hearing a verbal label before the simple detection task changed performance relative to an uninformative cue baseline. Valid labels improved performance relative to no-label baseline trials. Invalid labels decreased performance. Labels affected both sensitivity (d ′) and response times. In addition, we found that the effectiveness of labels varied predictably as a function of the match between the shape of the stimulus and the shape denoted by the label. Together, the findings suggest that facilitated detection of invisible objects due to language occurs at a perceptual rather than semantic locus. We hypothesize that when information associated with verbal labels matches stimulus-driven activity, language can provide a boost to perception, propelling an otherwise invisible image into awareness.
Significant efforts have been directed to understanding the factors that influence the lineage commitment of stem cells. This paper demonstrates that cell shape, independent of soluble factors, has a ...strong influence on the differentiation of human mesenchymal stem cells (MSCs) from bone marrow. When exposed to competing soluble differentiation signals, cells cultured in rectangles with increasing aspect ratio and in shapes with pentagonal symmetry but with different subcellular curvature—and with each occupying the same area—display different adipogenesis and osteogenesis profiles. The results reveal that geometric features that increase actomyosin contractility promote osteogenesis and are consistent with in vivo characteristics of the microenvironment of the differentiated cells. Cytoskeletal-disrupting pharmacological agents modulate shape-based trends in lineage commitment verifying the critical role of focal adhesion and myosin-generated contractility during differentiation. Microarray analysis and pathway inhibition studies suggest that contractile cells promote osteogenesis by enhancing c-Jun N-terminal kinase (JNK) and extracellular related kinase (ERK1/2) activation in conjunction with elevated wingless-type (Wnt) signaling. Taken together, this work points to the role that geometric shape cues can play in orchestrating the mechanochemical signals and paracrine/autocrine factors that can direct MSCs to appropriate fates.
A recently published theory has suggested that a cloak of invisibility is in principle possible, at least over a narrow frequency band. We describe here the first practical realization of such a ...cloak; in our demonstration, a copper cylinder was "hidden" inside a cloak constructed according to the previous theoretical prescription. The cloak was constructed with the use of artificially structured metamaterials, designed for operation over a band of microwave frequencies. The cloak decreased scattering from the hidden object while at the same time reducing its shadow, so that the cloak and object combined began to resemble empty space.
Parity sheaves JUTEAU, DANIEL; MAUTNER, CARL; WILLIAMSON, GEORDIE
Journal of the American Mathematical Society,
10/2014, Volume:
27, Issue:
4
Journal Article
Peer reviewed
Open access
Given a stratified variety X If X Our framework applies to many stratified varieties arising in representation theory such as generalised flag varieties, toric varieties, and nilpotent cones. ...Moreover, parity sheaves often correspond to interesting objects in representation theory. For example, on flag varieties we recover in a unified way several well-known complexes of sheaves. For one choice of parity function we obtain the indecomposable tilting perverse sheaves. For another, when using coefficients of characteristic zero, we recover the intersection cohomology sheaves and in arbitrary characteristic the special sheaves of Soergel, which are used by Fiebig in his proof of Lusztig's conjecture.
The miniaturization of droplet manipulation methods has led to drops being proposed as microreactors in many applications of biology and chemistry. In parallel, microfluidic methods have been applied ...to generate monodisperse emulsions for applications in the pharmaceuticals, cosmetics, and food industries. To date, microfluidic droplet production has been dominated by a few designs that use hydrodynamic forces, resulting from the flowing fluids, to break drops at a junction. Here we present a platform for droplet generation and manipulation that does not depend on the fluid flows. Instead, we use devices that incorporate height variations to subject the immiscible interfaces to gradients of confinement. The resulting curvature imbalance along the interface causes the detachment of monodisperse droplets, without the need for a flow of the external phase. Once detached, the drops are self-propelled due to the gradient of surface energy. We show that the size of the drops is determined by the device geometry; it is insensitive to the physical fluid properties and depends very weakly on the flow rate of the dispersed phase. This allows us to propose a geometric theoretical model that predicts the dependence of droplet size on the geometric parameters, which is in agreement with experimental measurements. The approach presented here can be applied in a wide range of standard applications, while simplifying the device operations. We demonstrate examples for single-droplet operations and high-throughput generation of emulsions, all of which are performed in simple and inexpensive devices.
Let V be a finite dimensional complex vector space and W ⊆ GL(V) be a finite complex reflection group. Let Vreg be the complement in V of the reflecting hyperplanes. We prove that Vreg is a K(π, 1) ...space. This was predicted by a classical conjecture, originally stated by Brieskorn for complexified real reflection groups. The complexified real case follows from a theorem of Deligne and, after contributions by Nakamura and Orlik-Solomon, only six exceptional cases remained open. In addition to solving these six cases, our approach is applicable to most previously known cases, including complexified real groups for which we obtain a new proof, based on new geometric objects. We also address a number of questions about π1(W\Vreg), the braid group of W. This includes a description of periodic elements in terms of a braid analog of Springer's theory of regular elements.
A light-sensitive, externally powered microchip was surgically implanted subretinally near the macular region of volunteers blind from hereditary retinal dystrophy. The implant contains an array of ...1500 active microphotodiodes (‘chip’), each with its own amplifier and local stimulation electrode. At the implant's tip, another array of 16 wire-connected electrodes allows light-independent direct stimulation and testing of the neuron–electrode interface. Visual scenes are projected naturally through the eye's lens onto the chip under the transparent retina. The chip generates a corresponding pattern of 38 × 40 pixels, each releasing light-intensity-dependent electric stimulation pulses. Subsequently, three previously blind persons could locate bright objects on a dark table, two of whom could discern grating patterns. One of these patients was able to correctly describe and name objects like a fork or knife on a table, geometric patterns, different kinds of fruit and discern shades of grey with only 15 per cent contrast. Without a training period, the regained visual functions enabled him to localize and approach persons in a room freely and to read large letters as complete words after several years of blindness. These results demonstrate for the first time that subretinal micro-electrode arrays with 1500 photodiodes can create detailed meaningful visual perception in previously blind individuals.
Cryo-EM structure of a 3D DNA-origami object Bai, Xiao-chen; Martin, Thomas G; Scheres, Sjors H. W ...
Proceedings of the National Academy of Sciences - PNAS,
12/2012, Volume:
109, Issue:
49
Journal Article
Peer reviewed
Open access
A key goal for nanotechnology is to design synthetic objects that may ultimately achieve functionalities known today only from natural macromolecular complexes. Molecular self-assembly with DNA has ...shown potential for creating user-defined 3D scaffolds, but the level of attainable positional accuracy has been unclear. Here we report the cryo-EM structure and a full pseudoatomic model of a discrete DNA object that is almost twice the size of a prokaryotic ribosome. The structure provides a variety of stable, previously undescribed DNA topologies for future use in nanotechnology and experimental evidence that discrete 3D DNA scaffolds allow the positioning of user-defined structural motifs with an accuracy that is similar to that observed in natural macromolecules. Thereby, our results indicate an attractive route to fabricate nanoscale devices that achieve complex functionalities by DNA-templated design steered by structural feedback.
A Perinuclear Actin Cap Regulates Nuclear Shape Khatau, Shyam B.; Hale, Christopher M.; Stewart-Hutchinson, P. J. ...
Proceedings of the National Academy of Sciences - PNAS,
11/2009, Volume:
106, Issue:
45
Journal Article
Peer reviewed
Open access
Defects in nuclear morphology often correlate with the onset of disease, including cancer, progeria, cardiomyopathy, and muscular dystrophy. However, the mechanism by which a cell controls its ...nuclear shape is unknown. Here, we use adhesive micropatterned surfaces to control the overall shape of fibroblasts and find that the shape of the nucleus is tightly regulated by the underlying cell adhesion geometry. We found that this regulation occurs through a dome-like actin cap that covers the top of the nucleus. This cap is composed of contractile actin filament bundles containing phosphorylated myosin, which form a highly organized, dynamic, and oriented structure in a wide variety of cells. The perinuclear actin cap is specifically disorganized or eliminated by inhibition of actomyosin contractility and rupture of the LINC complexes, which connect the nucleus to the actin cap. The organization of this actin cap and its nuclear shape-determining function are disrupted in cells from mouse models of accelerated aging (progeria) and muscular dystrophy with distorted nuclei caused by alterations of A-type lamins. These results highlight the interplay between cell shape, nuclear shape, and cell adhesion mediated by the perinuclear actin cap.
Neuronal code for extended time in the hippocampus Mankin, Emily A; Sparks, Fraser T; Slayyeh, Begum ...
Proceedings of the National Academy of Sciences,
11/2012, Volume:
109, Issue:
47
Journal Article
Peer reviewed
Open access
The time when an event occurs can become part of autobiographical memories. In brain structures that support such memories, a neural code should exist that represents when or how long ago events ...occurred. Here we describe a neuronal coding mechanism in hippocampus that can be used to represent the recency of an experience over intervals of hours to days. When the same event is repeated after such time periods, the activity patterns of hippocampal CA1 cell populations progressively differ with increasing temporal distances. Coding for space and context is nonetheless preserved. Compared with CA1, the firing patterns of hippocampal CA3 cell populations are highly reproducible, irrespective of the time interval, and thus provide a stable memory code over time. Therefore, the neuronal activity patterns in CA1 but not CA3 include a code that can be used to distinguish between time intervals on an extended scale, consistent with behavioral studies showing that the CA1 area is selectively required for temporal coding over such periods.
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