A comparative transcriptomic and metabolomic analysis of Polygonum cuspidatum leaves treated with MeJA was carried out to investigate the regulatory mechanisms of its active compounds. A total of 692 ...metabolites and 77,198 unigenes were obtained, including 200 differentially accumulated metabolites and 6819 differentially expressed genes. We screened potential regulatory transcription factors involved in resveratrol and flavonoids biosynthesis, and successfully identified an MYB transcription factor, PcMYB62, which could significantly decrease the resveratrol content in P. cuspidatum leaves when over-expressed. PcMYB62 could directly bind to the MBS motifs in the promoter region of stilbene synthase (PcSTS) gene and repress its expression. Besides, PcMYB62 could also repress PcSTS expression and resveratrol biosynthesis in transgenic Arabidopsis thaliana. Our results provide abundant candidate genes for further investigation, and the new finding of the inhibitory role of PcMYB62 on the resveratrol biosynthesis could also potentially be used in metabolic engineering of resveratrol in P. cuspidatum.
Tujuan penelitian ini adalah untuk mendapatkan bukti empiris tentang pengaruh kecepatan reaksi tangan, terhadap kemampuan forehand topspin.. Penelitian ini dilakukan pada atlet tenis meja Unsika ...Karawang. Keseluruhan populasi berjumlah 56 orang, sampel dipilih dengan menggunakan teknik pertimbangan/ purposive sampling, dan sampel dalam penelitian ini berjumlah 30 atlet tenis meja. Hasil tes kecepatan reaksi tangan berpengaruh langsung terhadap kemampuan forehand tospin sebesar 0,0957. Penelitian ini menggunakan pendekatan kuantitatif asosiatif dengan teknik analyisis jalur. Hasil mengungkapkan bahwa; terdapat pengaruh positif secara langsung kecepatan reaksi tangan terhadap kemampuan forehand topspin. Kata kunci; Kecepatan Reaksi Tangan, Forehand Topspin
This study aims to determine the results and analyze table tennis service skills in students who take table tennis extracurricular at SMP Negeri 19 Palembang. This research is a quantitative ...descriptive research. The research sample was 15 students who took part in table tennis extracurricular activities. The results of this study are the forehand service skills of students with good classification as many as (3) people (20%), moderate classification (9) people (60%), poor classification as many as (3) people (20%). While the backhand service skills are very good classification students as many as (1) people (7%), good classification as many as (4) people (27%), moderate classification (8) people (53%), poor classification as many as (2) people ( 13%). Thus, it can be concluded that the results of the forehand and backhand service level of table tennis students who participate in table tennis extracurricular activities at SMP Negeri 19 Palembang can be categorized as moderate with an average forehand service of 5.5, while the average result of backhand serving is 4, 8.
The results of this study aim to see the role of multiball how much it increases backhand stroke skills in table tennis and how the role of multiball exercises is to improve backhand stroke skills in ...table tennis games. The research was conducted by random sampling to a population of all Sparta athletes, totaling 45 people. Data collection using the method of test and measurement techniques. The test instrument used is multiball to determine the effect of multiball training. The variables in this study include Multiball as the independent variable, as well as improving backhand skills as the dependent variable. The data analysis technique uses descriptive analysis techniques and inferential analysis through the SPSS.22 program with a significance level of 95% (α = 0.05)The results of the research analysis show that: (1) multiball training is proven to increase backhand shots with a contribution of 64.5%;(2) The multiball training group has a greater effect on increasing backhand strokes than the control with an increase in out shuffle-plyo push ups of 12.85% of the average pretest carried out before treatment with an average posttest, while for the control it is only 3.9 % of the average pretest and posttest scores.
•MeJA treatment improved the cold resistance of green bell pepper.•MeJA treatment changed the membrane lipid component of green bell pepper.•MeJA treatment reduced CI of green bell pepper by ...regulating membrane lipid metabolism.
The effects of methyl jasmonate (MeJA) treatment on membrane lipid metabolism of green bell pepper during cold storage was studied. The results showed that the chilling injury (CI) index, electrolyte leakage, malondialdehyde (MDA) content, phospholipase D (PLD) activity and gene expression of green bell pepper treated with MeJA were lower than those of the control fruit. MeJA treatment was able to maintain a higher level of ascorbic acid (VC) content, higher levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS) and lower levels of digalactosyldiacylglycerol (DGDG), phosphatidic acid (PA), digalactosyldiacylglycerol/monogalactosyldiacylglycerol (DGDG/MGDG) and PC/PE compared to control fruit. In addition, MeJA treatment can also increase the proline content of green bell pepper fruit. Therefore, the reason that MeJA can improve the cold resistance of green bell pepper fruit may be attributed by the decreased expression and activity of PLD gene. The degradation of membrane lipid was delayed, and the content of proline increased, thereby maintaining cell membrane stability.
Fasilitas dalam bekerja dapat berpengaruh pada efisiensi dan kenyamanan dalam melakukan pekerjaan. Meja QC adalah salah satu fasilitas yang dapat menunjang pekerjaan yang perlu diperbaiki agar sesuai ...dengan kebutuhan dan juga dapat memberikan kenyamanan dalam bekerja. Penelitian ini bertujuan untuk mengembangkan produk meja quality control yang mengintegrasikan metode Kano dan Quality Function Deployment (QFD), serta mempertimbangkan aspek ergonomi. Metode Kano dipilih agar dapat menentukan prioritas dari fitur yang akan dikembangkan, dan metode QFD dipilih agar dapat menentukan hubungan antara kebutuhan responden dengan respon teknis yang dilakukan oleh tim pengembang. Hasil analisis menunjukkan bahwa prioritas pengembangan dari meja QC adalah tinggi meja yang dapat diatur, tahan karat, dan kaki meja dengan tinggi yang sejajar. Dalam proses perancangan, penekanan khusus diberikan pada aspek ergonomi untuk memastikan kenyamanan dan efisiensi penggunaan meja. Hasil dari penelitian ini adalah: pertama, menambahkan hidrolis pada kaki meja, sehingga tinggi meja dapat diatur sesuai kebutuhan pengguna. Selanjutnya, menggunakan keramik granit untuk bagian atas permukaan meja, memberikan keindahan dan tahan karat. Juga menambahkan laci pada meja untuk menyimpan alat-alat QC dengan rapi. Selain itu, menambahkan handle di kedua sisi meja agar mudah dipindahkan dan diposisikan ulang. Pembatas meja juga dilengkapi dengan engsel untuk memudahkan dan memberikan kenyaman saat pengguna menulis. Terakhir, menambahkan kereta dorong untuk membantu memudahkan pekerjaan QC, sehingga pengguna dapat bekerja dengan lebih nyaman.
Hidroponik DFT adalah suatu metode hidroponik di mana akar tanaman berada dalam larutan nutrisi dengan ketinggian 3-4 cm, yang tersirkulasi secara kontinu selama 24 jam. Selama ini siswa SMAN 1 Batur ...belum mengetahui tentang hidroponik, terutama dalam pembuatan konstruksi model hidroponik. Dengan demikian, para siswa belum memiliki keterampilan pada salah satu program double track SMAN 1 Batur, yaitu bidang budidaya tanaman hidroponik. Oleh karena itu perlu dilakukan pelatihan pembuatan hidroponik DFT model meja, yang merupakan model hidroponik sederhana konstruksinya dan mudah dibuat, dengan tujuan memberikan pengetahuan, keterampilan, dan meletakkan dasar jiwa kewirausahaan pada siswa. Metode yang digunakan adalah penyuluhan tentang teori hidroponik dan pelatihan tentang praktik pembuatan hidroponik DFT model meja. Hasilnya yaitu pada akhir kegiatan tingkat pengetahuan siswa meningkat 88%-100% dengan rata-rata 94%, sedang tingkat keterampilan siswa meningkat 82%-100% dengan rata-rata 92,5%. Hal ini menunjukkan bahwa para siswa SMAN 1 Batur mampu menyerap materi penyuluhan dan pelatihan yang diberikan
•Application of MeJA significantly enhanced salt tolerance of okra plants.•H2O2 acted a toxic accumulation of ROS rather than as a second messenger for signal transduction under salt stress of okra ...plants.•Exogenous MeJA increased the accumulation of JA, ABA and proline, which enhances salt tolerance of okra plants, by regulating endogenous hormones metabolism and osmotic adjustment substances, photosynthesis pigment and ROS metabolism.
Methyl jasmonate (MeJA), as a plant growth regulator, which is involves in many morphological and physiological processes, plays an important role in defense systems of plants under stress conditions. However, the roles of physiological and biochemical mechanisms of enhancing salt tolerance of okra plants based on application of MeJA is poorly understood. In this study, the application of the investigated 50 μM MeJA significantly improved salt tolerance of okra plants based on the germination rates and culture pot experiment under salt stress. Based on optimal concentration of MeJA inducing salt tolerance of okra plants, application of 5 mM diphenyleneiodonium (NADPH oxidase inhibitor, DPI) and 50 μM MeJA + 5 mM DPI, respectively, significantly increased salt tolerance of okra plants. Assays on the content of component matter indicated the significant increases of JA, ABA, proline, soluble protein, soluble sugar, photosynthetic pigment and relative water, glutathione (GSH) and ascorbic acid (AsA) content and the significant decreases of hydrogen peroxide (H2O2), superoxide radicals (O2−) and malondialdehyde (MDA) content and electrolyte leakage rates were observed in okra plants with 50 μM MeJA under salt stress. The activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), glutathione reductase (GR), ascorbate peroxidase (APX) and peroxidase (POD) related to ROS scavenging were also enhanced in okra plants by applied MeJA under salt stress. Furthermore, the marked decreases of H2O2, O2- and MDA content, and the significant increases of CAT, GR and APX activities and GSH and ASA content involved in ASA-GSH cycle were observed in MeJA-okra, DPI-okra and MeJA+DPI-okra plants under salt stress. H2O2 acted a toxic accumulation of ROS rather than as a second messenger for signal transduction in okra plants. These results demonstrate that exogenous MeJA increases the accumulation of JA, ABA and proline, which enhances salt tolerance of okra plants, by regulating osmotic balance and protecting membrane integrity and photosynthesis and activating ROS scavenging system. As a conclusion, treating okra plants with 50 μM MeJA could alleviate the harmful effects of abiotic stresses.
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•The effect of preharvest MeJA treatments on berry growth and ripening was evaluated.•MeJA at 10 mM delayed berry ripening, while 1, 0.1 and 0.01 mM accelerated it.•1, 5 and 10 mM ...MeJA treatments decreased vine total yield, in a dose-dependent way.•Total yield was increased by 0.1 and 0.01 mM MeJA doses.•1, 0.1 and 0.01 mM MeJA treatments increased berry quality and bioactive compounds.
In the present research the effect of preharvest metyil jasmonate (MeJA) treatment on the ripening process and fruit quality parameters at harvest was evaluated, for the first time, in two table grape cultivars, ‘Magenta’ and ‘Crimson’, during two years, 2016 and 2017. MeJA treatments (applied when berry volume was ca. 40% of its final one, at veraison and 3 days before the first harvest date) affected grape ripening process and vine yield differently depending on applied concentration. Thus, MeJA at 5 and 10 mM delayed berry ripening and decreased berry weight and volume as well as vine yield, in a dose-dependent way, in both cultivars, although the effect on ‘Crimson’ was more dramatic than in ‘Magenta’. However, treatments with MeJA at 1, 0.1 and 0.01 mM accelerated ripening and increased total phenolics and individual anthocyanin concentrations, the major effects being obtained with 0.1 mM concentration. In addition, total soluble solids (TSS) and firmness levels were also increased by these MeJA treatments. These results might have a great agronomic and commercial importance since fruit with higher size and harvested earlier would reach higher prizes at markets and berries with higher firmness and TSS would be more appreciated by consumers. Moreover, MeJA treatments increased the content of antioxidant compounds, such as phenolics and individual anthocyanins, leading to enhance the homogeneous pigmentation of the whole cluster, with additional effects on increasing the health beneficial effects of grape consumption.
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