Cancer cells overexpress IFsub.1, the endogenous protein that inhibits the hydrolytic activity of ATP synthase when mitochondrial membrane potential (Δμsub.H sup.+) falls, as in ischemia. Other roles ...have been ascribed to IFsub.1, but the associated molecular mechanisms are still under debate. We investigated the ability of IFsub.1 to promote survival and proliferation in osteosarcoma and colon carcinoma cells exposed to conditions mimicking ischemia and reperfusion, as occurs in vivo, particularly in solid tumors. IFsub.1-silenced and parental cells were exposed to the FCCP uncoupler to collapse Δμsub.H sup.+ and the bioenergetics of cell models were validated. All the uncoupled cells preserved mitochondrial mass, but the implemented mechanisms differed in IFsub.1-expressing and IFsub.1-silenced cells. Indeed, the membrane potential collapse and the energy charge preservation allowed an increase in both mitophagy and mitochondrial biogenesis in IFsub.1-expressing cells only. Interestingly, the presence of IFsub.1 also conferred a proliferative advantage to cells highly dependent on oxidative phosphorylation when the uncoupler was washed out, mimicking cell re-oxygenation. Overall, our results indicate that IFsub.1, by allowing energy preservation and promoting mitochondrial renewal, can favor proliferation of anoxic cells and tumor growth. Therefore, hindering the action of IFsub.1 may be promising for the therapy of tumors that rely on oxidative phosphorylation for energy production.
Printed Circuit Boards (PCBs) are the foundational component of electronic devices, and the detection of PCB defects is essential for ensuring the quality control of electronic products. Aiming at ...the problem that the existing PCB plug-in solder defect detection algorithms cannot meet the requirements of high precision, low false alarm rate, and high speed at the same time, this paper proposes a method based on spatial convolution pooling and information fusion. Firstly, on the basis of YOLOv3, an attention-guided pyramid structure is used to fuse context information, and multiple convolutions of different size are used to explore richer high-level semantic information; Secondly, a coordinated attention network structure is introduced to calibrate the fused pyramidal feature information, highlighting the important feature channels, and reducing the adverse impact of redundant parameters generated by feature fusion; Finally, the ASPP (Atrous Spatial Pyramid Pooling) structure is implemented in the original Darknet53 backbone feature extraction network to acquire multi-scale feature information of the detection targets. With these improvements, the average detection accuracy of the enhanced network has been elevated from 94.45 to 96.43%. This experiments shows that the improved network is more suitable for PCB plug-in solder defect detection applications.
Six Sigma is a management program that provides tools that help manufacturers obtain efficient, stream-lined production to coincide with ultimate high quality products. Lean Six Sigma will show how ...the well-regarded analytical tools of Six Sigma quality control can be successfully brought into the well-established models of 'lean manufacturing', bringing efficient, stream-lined production and high quality product readily together. This book offers a thorough, yet concise introduction to the essential mathematics of Six Sigma, with solid case examples from a variety of industrial settings, culminating in an extended case study. Various professionals will find this book immensely useful, whether it be the industrial engineer, the industrial manager, or anyone associated with engineering in a technical or managing role. It will bring about a clear understanding of not only how to implement Six Sigma statistical tools, but also how to do so within the bounds of Lean manufacturing scheme. It will show how Lean Six Sigma can help reinforce the notion of 'less is more', while at the same time preserving minimal error rates in final manufactured products.
Recent studies in the field of laboratory medicine revealed that pre analytical phase is the error prone area and source of 60-65% laboratory errors. The satisfactory fulfillment of requirements of ...ISO 15189:2012 is assuring Internal Quality Control but to assess such activity an external assessment program is necessary. An external assessment program (PRE-EQC) has been started and findings of every performance is being send to the participants along with the results. The PRE-EQC program is a simultaneous Proficiency Testing scheme. Pooled samples are collected, preservative added to the sample. The analytes to be tested are tested by the PRE-EQC Coordinator. Samples are sent to the participants within PT provider's time frame. The method is a 4 step method: A set of questionnaires on sample handling. Measurement of one/two analytes to check integrity of analytes. Simulation of errors. Coordinators feedback along with assessment sheet. The assessment sheets are sent to the participants by e mail. PT provider writes comment so that participant can locate the area requires continuous monitoring. The basic rule of PRE-EQC is to create the program in such a manner which can be monitored without remaining present in the participant laboratory as direct act of monitoring is more audit than external assessment. The questionnaire and simulation of error has been done in such manner so that it reflect the status of the laboratory.
ACBI/CMC EQAS--QUO VADIS? Geethanjali, F.S; Selvakumar, R
Indian journal of clinical biochemistry,
05/2022, Volume:
27, Issue:
S1
Journal Article
Peer reviewed
Objective: The objective of this retrospective study is to evaluate the usefulness of the ACBI/CMC EQAS program in the overall performance of Indian Laboratories as well as individual labs in ...different categories over the years. It is the journey of our National EQAS over the past decade. Method: The awareness of the Indian labs towards quality assurance is evaluated based on the number of participants, the response in submitting results, annual evaluation and the trend in methods selection. Similarly few labs form each category such as lab size (number of parameters done), type (public/private) and few labs at random were selected and their performance is evaluated over the past 10 years. Results: The awareness of Quality assurance in India has tremendously increased and is reflected by a 100% increase in the number of participants over the past ten years. The randomly selected labs showed decreased OMVIS which is indirectly proportional to their performance. Conclusion: It was during this past 10 years, lot of changes took place in the ACBI/CMC EQAS, i.e., change over form bovine sera to human sera, liquid samples to lyophilised samples, introducing immunoassay program etc. This study shows how the Indian laboratories have improved their performance as well as their awareness towards the QA-concept. It is very encouraging to note that the concept of EQAS is well accepted and appreciated by majority of laboratories and thus paving the way towards international standards.
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