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Izolacija in čiščenje rekombinantnih proteinov = Isolation and purification of recombinant proteinsGantar, Mateja, farmacija ; Štrukelj, BorutIzolacija in čiščenje rekombinantnih proteinov iz bioprocesne brozge je pomemben del pri pripravi biotehnoloških produktov. Želeni protein ločimo od nečistot postopoma, z več zaporednimi koraki. ... Izbira metod čiščenja in njihovega zaporedja je pomembna naloga. V splošnem se čiščenje začne z bisrtitvijo bioprocesne brozge. Sledi izolacija, koncentriranje in stabilizacija produkta. Z nadaljnimi kromatografskimi koraki odstranimo večinonečistot, tudi DNK, endtoksine in viruse. S končnim čiščenjem pa odstranimo še rezidualne nečistote in različne oblike molekule. Pri načrtovanju procesa čiščenja, moramo definirati glavne nečistote v izhidnem materialu ter poznati fizikalne, kemijske in biokemijske lastnosti produkta.Source: Farmacevtski vestnik : strokovno glasilo slovenske farmacije = pharmaceutical journal of Slovenia. - ISSN 0014-8229 (Let. 53, št. 2, 2002, str. 83-93)Type of material - article, component partPublish date - 2002Language - slovenianCOBISS.SI-ID - 1148017
Author
Gantar, Mateja, farmacija |
Štrukelj, Borut
Topics
biotehnološki produkti |
rekombinatni proteini |
izolacija |
čiščenje |
kromatografija
source: Farmacevtski vestnik : strokovno glasilo slovenske farmacije = pharmaceutical journal of Slovenia. - ISSN 0014-8229 (Let. 53, št. 2, 2002, str. 83-93)
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Gantar, Mateja, farmacija | 20670 |
Štrukelj, Borut | 07849 |
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