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Dokazovanje protiteles proti patogenim mikroorganizmom v tekočinah kokošjega zarodka = Detection of antibodies to pathogenic microorganisms in the chicken embryo's fluidsHerman, Darja ; Narat, Mojca ; Benčina, DušanPred patogenimi mikroorganizmi, ki se prenašajo prek valilnih jajc, kokošji zarodek ščitijo protitelesa matere v rumenjaku in beljaku. Z uporabo imunoencimskih testov in monoklonskih protiteles (mAb) ... proti kokošjim imunoglobulinom razredov G (IgG), IgA in IgM smo ugotavljali prisotnost specifičnih protiteles proti Mycoplasma synoviae, M. gallisepticum, Ornithobacterium rhinotracheale in virusu atipične kokošje kuge v alantoisni tekočini (ALT), amnionski tekočini (AMT), žolču in krvi kokošjih zarodkov na različnih stopnjah embrionalnega razvoja. Že 7. dan embrionalnega razvoja je večina zarodkov imela v ALT specifična protitelesa IgG proti M. synoviae, nekateri zarodki pa tudi protitelesa IgG proti M. gallisepticum in virusu atipične kokošje kuge. V ALT so bila pri nekaterih zarodkih prisotna tudi protitelesa razreda IgA in IgM. Protitelesa IgA in IgM so se najverjetneje prenesla iz beljaka v ALT. V AMT smo specifična protitelesa IgG proti M. synoviae dokazali 7. dan embrionalnega razvoja. Količina IgA, IgG in IgM v AMT se je močno povečala po 12. dnevu embrionalnega razvoja. Specifična protitelesa lahko pridejo tudi v žolč, kjer smo protitelesa IgA in IgG proti M. synoviae in O. rhinotracheale dokazali pri 13 dni starih zarodkih. Tudi v vzorcih krvi, vzetih iz alantoisnega krvnega obtoka od 13. do 16. dne embrionalnega razvoja, smo dokazali IgG in IgA.Source: Zbornik Biotehniške fakultete Univerze v Ljubljani. Kmetijstvo. Zootehnika = Research reports [of the] Biotechnical Faculty University of Ljubljana. Agricultural issue. Zootechnica. - ISSN 1408-3434 (Letn. 78, št. 2, 2001, str. 127-135)Type of material - article, component partPublish date - 2001Language - slovenianCOBISS.SI-ID - 1226632
Author
Herman, Darja |
Narat, Mojca |
Benčina, Dušan
Topics
perutnina |
kokošji zarodek |
imunologija |
imunoglobulini |
alantoisna tekočina |
amnionska tekočina |
žolč |
poultry |
chicken embryo |
immunology |
immunoglobulins |
allantoic fluid |
amniotic fluid |
bile
Shelf entry
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Herman, Darja | 22458 |
Narat, Mojca | 05008 |
Benčina, Dušan | 07914 |
Source: Personal bibliographies
and: SICRIS
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