-
Vloga katepsinov in njihovih inhibitorjev pri razvoju raka - model celičnih linij z različno tumorigenostjo : magistrsko deloBervar, AlešBiological processes involved in the progression from benign to malignant and invasive tumors are still not sufficiently understood. The availability of molecular studies involving patients (i.e. in ... vivo) is substantially limited by ethic, health and limited-time issues. This is the reason for the use of invitro models immitating in vivo processes as closely as possible. Cell linescan be used as an approximation of tumors of different tumorigenicity. They are, at least in theory, stable over many generations, and can be produced in required quantities over a short period of time. In our study, we adopted an established cell line-model consisting of a parental non-tumorigenic cell line MCF10A, a ras-transfected cell line MCF10A-NeoT withlow tumorigenicity (it forms pre-malignant lesions in nude mice), and of two highly in vivo tumorigenic cell lines - MCF10A-Cala and MCF10A-Cald (they were prepared from tumors formed after inoculation of nude mice with tumor cells). Our work was focused on the role of lysosomal cysteine proteinases (CatB and CatL) and their endogenous inhibitors (StA and StB) in transition from non-tumorigenic to tumorigenic cell lines. The principal role for cathepsins is supposed to be the degradation of extracellular matrix and basalmembrane proteins and activation of other proteases involved in these processes. Increased concentrations of cathepsins and changes in proteinase/inhibitor equilibrium have already been correlated with worse prognosis for different tumor types. Therefore, we speculated that the levels of cathepsins and stefins correlate with cell linetumorigenicity. We compared the expression of cathepsins and stefins at mRNA, protein concentration and activity levels. Their expression and tumorigenicity in vivo were further correlated with in vitro invasiveness - the ability of cells to invade throughcomponents of extracellular matrix. (Abstract truncated at 2000 characters.)Type of material - master's thesis ; adult, seriousPublication and manufacture - Ljubljana : [A. Bervar], 2000Language - slovenianCOBISS.SI-ID - 624975
Author
Bervar, Aleš
Other authors
Lah Turnšek, Tamara
Topics
Cathepsins |
Physiology |
Cathepsins |
Antagonists and inhibitors |
Neoplasms |
Enzymology |
Tumor cells, cultured |
Physiology |
Tumor cells, cultured |
Enzymology |
Katepsini |
Fiziologija |
Katepsini |
Antagonisti in inhibitorji |
Novotvorbe |
Encimologija |
Tumorske celice, kultivirane |
Fiziologija |
Tumorske celice, kultivirane |
Encimologija |
katepsini |
rak (medicina) |
celične linije |
tumorji
Library | Call number – location, accession no. ... | Copy status |
---|---|---|
MF, Central Medical Library, Lj. | magistrske naloge BERVAR Aleš Vloga IN: 020000901 |
available - outside loan, loan period: 1 months |
National Institute of Biology and BF, Department of Biology, Ljubljana | BF oddelek za biologijo mg 616-006 BERVAR A. Vloga IN: 0035427 |
available - reading room |
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Bervar, Aleš | 18466 |
Lah Turnšek, Tamara | 07802 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.