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Chen, Shi-Heng; Wu, Cui-Fang; Chen, Ben-Mei; Pei, Qi; Tan, Hong-Yi; Yang, Li; Yang, Guo-Ping
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 28, Issue: 6Journal Article
To establish a simple and rapid HPLC-MS method for determining the contents of olmesartan in human plasma. Plasma were precipitated with trifluoroacetic acid, then analyzed on an HyPurity C(18) column (150 mm 2.1 mm, 5 microm). Samples at 40 degrees celsius;. The mobile phase consisted of water-methanol- acetonitrile(14:60:26) with a flow rate of 0.22 ml/min. The lower limit of qualification was 25 microg/L. The calibration curve was linear over the range of 25-3200 microg/L (r=0.9998), with the intra-day and inter-day RSD less than 15%. The method is sensitive, rapid and suitable for the study of pharmacokinetics and bioavailability of olmesartan.
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