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Smith, Brian P; Rigby, Megan; Ma, Roger; Maciag, Anna E
Methods in molecular biology (Clifton, N.J.), 2024, Volume: 2797Journal Article
With recent advances proving that effective inhibition of KRAS is possible, there have been significant efforts made to develop inhibitors of specific mutant alleles. Here we describe a detailed protocol that employs homogeneous time-resolved fluorescence (HTRF) to identify compounds acting on KRAS signaling in malignant cell lines. This method allows for high-throughput, cell-based screens of large compound libraries for the development of RAS-targeted therapeutics.
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