In this paper, we present the first 100-Gb/s/λ-based coherent wavelength division multiplexing passive optical network (WDM-PON) prototype system, highlighting a real-time 100-Gb/s coherent transceiver with a simplified digital signal processing suitable for access spans, and a new automatic-gain-controlled erbium-doped fiber amplifier based preamplifier with an amplified spontaneous emission compensation function for the upstream to improve the minimum receiver sensitivity for coherent detection, especially at very low received signal power. Thanks to our proposed technologies, this first demonstration achieved an increased bidirectional loss budget of more than 39.1 dB, which supports 80 km transmission with eight optical network unit splits, with an improved upstream receiver sensitivity of -38.1 dBm. In addition, to show the feasibility of the proposed 100-Gb/s/ λ-based coherent WDM-PON as a promising candidate for forthcoming 5G mobile fronthaul networks, we further investigate experimentally a 128-kb/s auxiliary management and control channel (AMCC) superimposed on the 100-Gb/s dual polarization quadrature phase-shift keying (DP-QPSK) signals employed by the 100-Gb/s/λ -based coherent WDM-PON system. By setting the modulation index of the AMCC signals between 5% and 40%, we also successfully demonstrated 100-Gb/s DP-QPSK signal transmission with only a small power penalty of 0.2 dB due to the introduction of the AMCC signal.