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Chen, Qingqing; Shan, Chaowen; Su, Jie; Chen, Wei; Zhu, Jiaming; Chen, Suhong; Lyu, Guiyuan
Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban, 2020-Dec-25, 20201225, Volume: 49, Issue: 6Journal Article
To investigate the effect of Chinese medicine Wubi Shanyao pills on sexual function of kidney-yang-deficiency mice induced by hydrocortisone. Male Kunming mice were injected with hydrocortisone for 10 days to prepare the kidney-yang-deficiency model, and administrated with Wubi Shanyao pills (0.91, 1.82, 2.73 g/kg) for 9 weeks. The general behaviors of mice (autonomous activity, grasping power) were observed; sexual behaviors (capture, straddle, ejaculation frequency and incubation period) of mice were detected by mating experiment. The serum levels of cortisol, adrenocorticotropic hormone (ACTH), luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E ) and testosterone were measured by ELIZA. The organ indexes of kidney, epididymis, testis and seminal vesicle were calculated. The histomorphology of testis was observed by HE staining. The apoptosis of testis cells was detected by TUNEL; and the expression level of proliferating cell nuclear antigen (PCNA) in testis was detected by immunohi
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