Objective: To observe the relationships between the electrocardiogram (ECG) and quiet biochemical, heart rate variability (HRV) and electroencephalogram (EEG) indicators, to establish the Index system for the risk prediction to graded exercise test (GXT) in men from 40 to 49yr. Materials: 173 men from 40-49 year of age (44.0±3.5yr) without cardiovascular disease and other diseases. Methods: Answered their physical activity (PA) by the international physical activity questionnaire (IPAQ). To test blood press (BP), heart rate (HR), pulse wave velocity (PWV), total cholesterol (TC), total triglyceride (TG), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c), myocardium enzymes, high sensitive C reactive protein (hCRP), observation pressure (OP) and low frequency/high frequency (LF/HF) in the quiet at the same day. The GXT was performed on a cycle ergo-meter with GXT starting with 25 w and adding 25 w at 2 min intervals until reached abnormal change in ST-segment (ST). The values of GXT, ECG and oxygen uptake (VO2) were recorded. To analyze the relationships between ST and other indictors by multiple regression analysis; To build the regression equations by stepwise regression. The significance was p<0.05; The highly significance was p<0.01. Results: 1: 28 men had abnormal change in ST; 2: For the abnormal men, HDL-c, OP of EEG and diastolic blood pressure (DBP) in the quiet state had relationships in ST of ECG, p<0.05. 3: The equation was Y=-0.446+0.004X1-0.042X2+0.002X3. Y was the change of ST, X1 was value of DBP, X2 was value of HDL-c, X3 was value of OP. R=0.733，R2=0.538. Conclusion: 1. The values of HDL-c, DBP and OP of EEG can be used to predict the abnormal change of ST in 40-49yr Chinese men in GXT.